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            <date>2025-06-04</date>
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            <revision version="1.0" date="2019-08-28">
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                            <category>chem_comp_bond</category>
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                            <item>_pdbx_entry_details.has_protein_modification</item>
                            <item>_struct_conn.pdbx_dist_value</item>
                            <item>_struct_conn.ptnr2_auth_seq_id</item>
                            <item>_struct_conn.ptnr2_label_asym_id</item>
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                    </model>
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            <revision version="1.3" date="2025-06-04">
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                    <model>
                        <revision_group>DATA_COLLECTION</revision_group>
                        <categories>
                            <category>em_admin</category>
                            <category>em_software</category>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-02-14</deposition>
            <header_release>2019-07-17</header_release>
            <map_release>2019-08-28</map_release>
            <update>2025-06-04</update>
        </key_dates>
        <title>Set2 bound to nucleosome</title>
        <authors_list>
            <author>Halic M</author>
            <author>Bilokapic S</author>
        </authors_list>
        <keywords>Set2, nucleosome, chromatin, KMT, GENE REGULATION</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-2199-2929" order="1">Bilokapic S</author>
                    <author ORCID="0000-0002-0061-7372" order="2">Halic M</author>
                    <title>Nucleosome and ubiquitin position Set2 to methylate H3K36.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>10</volume>
                    <first_page>3795</first_page>
                    <last_page>3795</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">31439846</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-019-11726-4</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6nzo</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Set2 nucleosome complex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Set2 nucleosome complex</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <molecular_weight>
                    <theoretical units="MDa">0.24</theoretical>
                </molecular_weight>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>Histone-lysine N-methyltransferase</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="759272">Chaetomium thermophilum</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>DNA</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="4">
                <name>Histone H3, Histone H4, Ubiquitin-60S ribosomal protein L40,Histone H2A, Histone H2B 1.1</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Histone H3</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.015437144</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVMKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK
TDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</string>
                    <external_references type="UNIPROTKB">A0A310TTQ1</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Histone H4</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.011394426</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRK
TVTAMDVVYALKRQGRTLYGFGG</string>
                    <external_references type="UNIPROTKB">P62799</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Ubiquitin-60S ribosomal protein L40,Histone H2A</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.024045436</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MDSPDLHHHHHHGTLVPRGSMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGTSSGGSGGSGGSGRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGN
AARDNKKTRIIPRHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK</string>
                    <external_references type="UNIPROTKB">P0CH09</external_references>
                    <external_references type="UNIPROTKB">Q6AZJ8</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>Histone H2B 1.1</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.013655948</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAH
YNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK</string>
                    <external_references type="UNIPROTKB">P02281</external_references>
                </sequence>
            </protein_or_peptide>
            <dna macromolecule_id="5">
                <name>DNA (149-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.045764141</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)
(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)
(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)
(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)
(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)
(DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)
(DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)
(DA)(DT)(DC)(DC)(DT)(DG)(DT)(DG)(DC)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <dna macromolecule_id="6">
                <name>DNA (149-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.046222434</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>(DG)(DC)(DA)(DC)(DA)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)
(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)
(DG)(DA)(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)
(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)
(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)
(DT)(DA)(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)
(DT)(DG)(DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)
(DA)(DT)(DT)(DC)(DT)(DC)(DC)(DA)(DG)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <protein_or_peptide macromolecule_id="7">
                <name>Histone-lysine N-methyltransferase</name>
                <natural_source database="NCBI">
                    <organism ncbi="759272">Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)</organism>
                    <strain>DSM 1495 / CBS 144.50 / IMI 039719</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.105425906</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MEASDRASGPTAGSKSEERTKQNGPRSKKEDASDSSTSTGSKSASRSSSATTMSPDDAKPADDSALAQENGVAPKPSRKS
SKLPPRNPPQLFNHLPDATEEACRTFQVISDCLYGSRNMGSSDHDALDCDCAEDWRDGKNHACGEDSDCINRATKIECVI
GDCNCGEGCQNQRFQRKQYAKVSVIKTEKKGYGLRADTDLQPNDFIYEYVGEVINEPTFRSRMLKYDKEGIKHFYFMSLT
KNEFVDATKKGNLGRFCNHSCNPNCYVDKWVVGDKLRMGIFAARYIKAGEELVFNYNVDRYGADPQPCYCGEPNCVGFIG
GKTQTERATKLPLATIEALGIEDGDSWDTTVAKKPRKKKASETDEEYVNSLQPKALDEDGVNKVMATLMQCKEKWIAVKL
LSRLQATQDDHLRHRVVRMHGYQILKSTLNAFKHDNNVVLQVLDILYNLPRITKNKISDSNIEAVVQPLASSSDERVAFE
AKRLLEEWDKLETAYRIPRKKDGRVVSAIANSFEEERRSNREEPTKPADPLANVVIPTGPRSNIPQRNANYYNGVRPRKP
PTNLPEGWFVTVDKKTGKYYFYDVNGKVQWQRPTAPAITTPKPSVKAQQDQKALQDIIDSLTKEPTPRQSAGHTPQRSST
PATEPKKEKWRSLPIEKQMKIYENTLFPHVKYVMDKFHGKLPREDLKKFARDVNKKLVASDYKHNRVQDPTVPLSSKQAK
KVRKYVYDFFDRALQKYLEYQKRKAQYTSKEGMQSSQTEQNTATPTGTLGKDVDEVMSDVEAPNSSPQSTSSAGRKRKRD
DDQDGDHDMRSPADEDAATSSDPPSVKRIKEDDGTGNDVIPSPPPPPPPPAEIPMTEEERSMREQEEALMRENEEAQRLE
DEAKRKQLELQNGLAAAKNAGIELGSASFTVSGEPMDVDNDGPPPQEQAQQQKQAVMSH</string>
                    <external_references type="UNIPROTKB">G0S676</external_references>
                </sequence>
                <ec_number>2.1.1.43</ec_number>
            </protein_or_peptide>
            <ligand macromolecule_id="8">
                <name>ZINC ION</name>
                <molecular_weight>
                    <theoretical units="MDa">6.5409e-05</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <formula>ZN</formula>
            </ligand>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <grid>
                        <details>unspecified</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">75.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <startup_model type_of_model="EMDB MAP"/>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.8</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>65000</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="55297">
        <file>emd_0559.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>240</col>
            <row>240</row>
            <sec>240</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>240</x>
            <y>240</y>
            <z>240</z>
        </spacing>
        <cell>
            <a units="Å">249.59999</a>
            <b units="Å">249.59999</b>
            <c units="Å">249.59999</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.049233016</minimum>
            <maximum>0.07451724</maximum>
            <average>0.00013642217</average>
            <std>0.002860413</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.04</x>
            <y units="Å">1.04</y>
            <z units="Å">1.04</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.012</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0559::::</label>
        <annotation_details>Set2 bound to the nucleosome</annotation_details>
    </map>
</emd>
