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    <admin>
        <current_status>
            <date>2024-11-06</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-02-11</deposition>
            <header_release>2019-03-06</header_release>
            <map_release>2019-03-27</map_release>
            <update>2024-11-06</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)</funding_body>
                <code>P41GM103832</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)</funding_body>
                <code>R01GM079429</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)</funding_body>
                <code>S10OD021600</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2b (OA-2b)</title>
        <authors_list>
            <author>Zhang K</author>
            <author>Zhang H</author>
        </authors_list>
        <keywords>Helicobacter pylori, vacuolating cytotoxin A, pore-forming toxin, TOXIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Zhang K</author>
                    <author order="2">Zhang H</author>
                    <author order="3">Li S</author>
                    <author order="4">Pintilie GD</author>
                    <author order="5">Mou TC</author>
                    <author order="6">Gao Y</author>
                    <author order="7">Zhang Q</author>
                    <author order="8">van den Bedem H</author>
                    <author order="9">Schmid MF</author>
                    <author order="10">Au SWN</author>
                    <author order="11">Chiu W</author>
                    <title>Cryo-EM structures ofHelicobacter pylorivacuolating cytotoxin A oligomeric assemblies at near-atomic resolution.</title>
                    <journal_abbreviation>Proc. Natl. Acad. Sci. U.S.A.</journal_abbreviation>
                    <country>US</country>
                    <volume>116</volume>
                    <first_page>6800</first_page>
                    <last_page>6805</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">30894496</external_references>
                    <external_references type="DOI">doi:10.1073/pnas.1821959116</external_references>
                    <external_references type="ISSN">1091-6490</external_references>
                    <external_references type="CSD">0040</external_references>
                    <external_references type="ASTM">PNASA6</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <emdb_id>EMD-0542</emdb_id>
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    <sample>
        <name>Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2b (OA-2b)</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2b (OA-2b)</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="210">Helicobacter pylori</organism>
                    <strain>60190</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.088</theoretical>
                </molecular_weight>
            </complex_supramolecule>
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            <protein_or_peptide macromolecule_id="1">
                <name>Vacuolating cytotoxin autotransporter</name>
                <natural_source database="NCBI">
                    <organism ncbi="210">Helicobacter pylori</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.08846321099999999</theoretical>
                </molecular_weight>
                <number_of_copies>12</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>AFFTTVIIPAIVGGIATGTAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAGKGFNEFPNKEYDLYKSLLSSKIDGGW
DWGNAATHYWIKGGQWNKLEVDMKDAVGTYKLSGLRNFTGGDLDVNMQKATLRLGQFNGNSFTSYKDSADRTTRVDFNAK
NILIDNFLEINNRVGSGAGRKASSTVLTLQASEGITSSKNAEISLYDGATLNLASNSVKLNGNVWMGRLQYVGAYLAPSY
STINTSKVTGEVNFNHLTVGDHNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPNNTPSQSGAKNDKQESSQ
NNSNTQVINPPNSTQKTEVQPTQVIDGPFAGGKDTVVNIDRINTKADGTIKVGGFKASLTTNAAHLNIGKGGVNLSNQAS
GRTLLVENLTGNITVDGPLRVNNQVGGYALAGSSANFEFKAGVDTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGN
GGFNTLDFSGVTNKVNINKLITASTNVAVKNFNINELIVKTNGVSVGEYTHFSEDIGSQSRINTVRLETGTRSIFSGGVK
FKSGEKLVIDEFYYSPWNYFDARNIKNVEITRKFASSTPENPWGTSKLMFNNLTLGQNAVMDYSQFSNLTIQGDFINNQG
TINYLVRGGKVATLNVGNAAAMMFNNDIDSATGFYKPLIKINSAQDLIKNTEHVLLKAKIIGYGNVSTGTNGISNVNLEE
QFKERLALYNNNNRMDTCVVRNTDDIKACGMAIGNQSMVNNPDNYKYLIGKAWKNIGISKTANGSKISVYYLGNSTPTEN
GGNTTNLPTNTTNNARFASYA</string>
                    <external_references type="UNIPROTKB">Q48245</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.2</concentration>
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <grid>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>CONTINUOUS</film_topology>
                        </support_film>
                        <details>unspecified</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                    <details>VacA OA-2b</details>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">70.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.3</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <nominal_magnification>130000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <coma_free/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Quantum LS</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <digitization_details>
                                <frames_per_image>1-30</frames_per_image>
                            </digitization_details>
                            <number_grids_imaged>3</number_grids_imaged>
                            <number_real_images>13708</number_real_images>
                            <average_exposure_time units="s">6.0</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">7.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>540214</number_selected>
                </particle_selection>
                <startup_model type_of_model="OTHER"/>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>D6</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.2</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                            <version>2</version>
                        </software>
                    </software_list>
                    <number_images_used>47009</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                            <version>2</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
                <final_three_d_classification>
                    <number_classes>10</number_classes>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2</version>
                        </software>
                    </software_list>
                </final_three_d_classification>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>384</col>
            <row>384</row>
            <sec>384</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
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        <spacing>
            <x>384</x>
            <y>384</y>
            <z>384</z>
        </spacing>
        <cell>
            <a units="Å">407.03998</a>
            <b units="Å">407.03998</b>
            <c units="Å">407.03998</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-1.7319936</minimum>
            <maximum>3.0986722</maximum>
            <average>0.008191676</average>
            <std>0.0902079</std>
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        <pixel_spacing>
            <x units="Å">1.06</x>
            <y units="Å">1.06</y>
            <z units="Å">1.06</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.36</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0544::::</label>
        <annotation_details>Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2b (OA-2b)</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>BACKBONE TRACE</refinement_protocol>
            </modelling>
        </modelling_list>
    </interpretation>
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