Structure of a MAPK pathway complex

2025-05-14 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name DATABASE_REFERENCES citation citation_author _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year STRUCTURE_SUMMARY chem_comp_atom chem_comp_bond database_2 em_admin pdbx_entry_details pdbx_modification_feature struct_conn struct_conn_type _database_2.pdbx_DOI _database_2.pdbx_database_accession _em_admin.last_update _struct_conn.conn_type_id _struct_conn.id _struct_conn.pdbx_dist_value _struct_conn.pdbx_leaving_atom_flag _struct_conn.ptnr1_auth_comp_id _struct_conn.ptnr1_auth_seq_id _struct_conn.ptnr1_label_atom_id _struct_conn.ptnr1_label_comp_id _struct_conn.ptnr1_label_seq_id _struct_conn.ptnr2_auth_comp_id _struct_conn.ptnr2_auth_seq_id _struct_conn.ptnr2_label_asym_id _struct_conn.ptnr2_label_atom_id _struct_conn.ptnr2_label_comp_id _struct_conn.ptnr2_label_seq_id _struct_conn_type.id DATA_COLLECTION em_admin em_software _em_admin.last_update _em_software.name RCSB RCSB 2019-02-11 2019-03-27 2019-10-09 2025-05-14 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) P50CA165962 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R50CA221830 United States Structure of a MAPK pathway complex Park E Rawson S TRANSFERASE Park E Rawson S Li K Kim BW Ficarro SB Pino GG Sharif H Marto JA Jeon H Eck MJ Architecture of autoinhibited and active BRAF-MEK1-14-3-3 complexes. Nature UK 575 545 550 2019 31581174 doi:10.1038/s41586-019-1660-y 1476-4687 0006 NATUAS 6nyb FULLOVERLAP Ternary complex of BRAF/MEK1/14-3-3 with MEK inhibitor Ternary complex of BRAF/MEK1/14-3-3 with MEK inhibitor 0 1 2 3

14-3-3 is heterodimer of Insect epsilon and zeta. model was generated by Insect zeta sequence as a homo-dimer.

Homo sapiens 0.19 Serine/threonine-protein kinase B-raf Homo sapiens 0.089402789 1 Spodoptera frugiperda LEVO MSYYHHHHHHHHDIPTTENLYFQGAMDMAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQM IKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPS SLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTD ISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSK FFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSS(SEP)APNV HINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDD WEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVT QWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQ FEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAM KRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSA(SEP)EPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPV HGTSAWSHPQFEK P15056 2.7.11.1 Dual specificity mitogen-activated protein kinase kinase 1 Homo sapiens 0.045934542999999994

GDC-0623

1 Spodoptera frugiperda LEVO MGSSHHHHHHSAVDENLYFQGGMPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQ KVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGE ISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDA MANAFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLS SYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCST IGLNQPSTPTHAAGV Q02750 2.7.12.2 14-3-3 protein zeta Spodoptera exigua 0.028108513999999998 2 LEVO MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQM AKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRNSVVEDSQKAYQDAFEISKA KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGDGDE PAEGGDN V9P4T4 PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER 0.0005232469999999999 1 AGS ZINC ION 6.5409e-05 2 ZN ADENOSINE-5'-DIPHOSPHATE 0.000427201 1 ADP MAGNESIUM ION 2.4305e-05 1 MG 5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide 0.00045620999999999997 1 LCJ singleParticle particle 0.05 7.4

unspecified

ETHANE FEI TITAN KRIOS OTHER OTHER FIELD EMISSION GUN 300 70.0 2.7 GATAN K2 SUMMIT (4k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 4.1 FSC 0.143 CUT-OFF 165298 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0541.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 211.99998 211.99998 211.99998 90.0 90.0 90.0 X Y Z -0.15667647 0.21985821 0.00056338793 0.0067066685 1.06 1.06 1.06 0.048 AUTHOR ::::EMDATABANK.org::::EMD-0541:::: Structure of a MAPK pathway complex