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            <date>2020-08-12</date>
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            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-02-08</deposition>
            <header_release>2019-02-20</header_release>
            <map_release>2019-08-14</map_release>
            <update>2020-08-12</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>R01GM08139</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institute of Health Midwest Consortium for High Resolution Cryo-electron Microscopy</funding_body>
                <code>U24 GM116789-01A1</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Science Foundation (NSF, United States)</funding_body>
                <code>Eager 1558128</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Averaged structure of Photosystem II holocomplex on thylakoid membrane from diatom</title>
        <authors_list>
            <author>Chen M</author>
            <author>Kuang X</author>
            <author>Levitan O</author>
            <author>Falkowski P</author>
            <author>Dai W</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Levitan O</author>
                    <author order="2">Chen M</author>
                    <author order="3">Kuang X</author>
                    <author order="4">Cheong KY</author>
                    <author order="5">Jiang J</author>
                    <author order="6">Banal M</author>
                    <author order="7">Nambiar N</author>
                    <author order="8">Gorbunov MY</author>
                    <author order="9">Ludtke SJ</author>
                    <author order="10">Falkowski PG</author>
                    <author order="11">Dai W</author>
                    <title>Structural and functional analyses of photosystem II in the marine diatomPhaeodactylum tricornutum.</title>
                    <journal_abbreviation>Proc.Natl.Acad.Sci.USA</journal_abbreviation>
                    <country>US</country>
                    <volume>116</volume>
                    <first_page>17316</first_page>
                    <last_page>17322</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">31409711</external_references>
                    <external_references type="DOI">doi:10.1073/pnas.1906726116</external_references>
                    <external_references type="ISSN">1091-6490</external_references>
                    <external_references type="CSD">0040</external_references>
                    <external_references type="ASTM">PNASA6</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <emdb_id>EMD-0539</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
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            <emdb_reference>
                <emdb_id>EMD-0540</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
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        </emdb_list>
    </crossreferences>
    <sample>
        <name>Thylakoid membrane purified from diatom</name>
        <supramolecule_list>
            <organelle_or_cellular_component_supramolecule supramolecule_id="1">
                <name>Thylakoid membrane purified from diatom</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="2850">Phaeodactylum tricornutum</organism>
                    <organelle>Thylakoid membrane</organelle>
                </natural_source>
            </organelle_or_cellular_component_supramolecule>
        </supramolecule_list>
    </sample>
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            <method>subtomogramAveraging</method>
            <aggregation_state>cell</aggregation_state>
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                <subtomogram_averaging_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <grid>
                        <details>unspecified</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <instrument>LEICA EM GP</instrument>
                        <details></details>
                    </vitrification>
                    <details>Thylakoid membrane purified from diatom.</details>
                </subtomogram_averaging_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <subtomogram_averaging_microscopy microscopy_id="1">
                    <microscope>FEI TALOS ARCTICA</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <specialist_optics>
                        <phase_plate>VOLTA PHASE PLATE</phase_plate>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 BASE (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">2.0</average_electron_dose_per_image>
                        </image_recording>
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                        <point_group>C2</point_group>
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                    <algorithm>FOURIER SPACE</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">26.0</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
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                        <software>
                            <name>EMAN2</name>
                            <version>2.3</version>
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                    <number_subtomograms_used>382</number_subtomograms_used>
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                    <number_tomograms>173</number_tomograms>
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                            <name>EMAN2</name>
                            <version>2.3</version>
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                    <software_list>
                        <software>
                            <name>EMAN2</name>
                            <version>2.3</version>
                        </software>
                    </software_list>
                </ctf_correction>
                <final_three_d_classification>
                    <number_classes>1</number_classes>
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                        <software>
                            <name>EMAN2</name>
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                            <name>EMAN2</name>
                            <version>2.3</version>
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                    <details>subtomogram refinement</details>
                </final_angle_assignment>
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            <space_group>1</space_group>
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            <row>128</row>
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        <label>::::EMDATABANK.org::::EMD-0539::::</label>
        <annotation_details>Averaged structure of photosystem II holocomplex on thylakoid membrane from diatom</annotation_details>
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                <initial_model>
                    <access_code>4YUU</access_code>
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                <refinement_protocol>RIGID BODY FIT</refinement_protocol>
                <target_criteria>correlation coefficient</target_criteria>
                <refinement_space>REAL</refinement_space>
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