2019-11-27 REL RCSB RCSB RCSB 2019-02-07 2019-03-06 2019-10-30 2019-11-27 National Institutes of Health/National Institute of General Medical Sciences GM080139 United States AcrAB-TolC from cellular tomogram - a complete data processing workflow for CryoET and subtomogram averaging Chen M Bell JM Shi X Wang Z Ludtke SJ Chen M Bell JM Shi X Sun SY Wang Z Ludtke SJ A complete data processing workflow for cryo-ET and subtomogram averaging. Nat.Methods US 16 1161 1168 2019 31611690 doi:10.1038/s41592-019-0591-8 1548-7105 EMD-0530 associated EM volume EMD-0529 other EM volume AcrAB-TolC multidrug efflux pump AcrAB-TolC multidrug efflux pump 0 Escherichia coli BL21(DE3) 0.74 tomography cell 7 Quantifoil R3.5/1 GOLD 200 ETHANE FEI VITROBOT MARK III
E. coli cell overexpressing AcrAB-TolC
Aurion 10 NO SECTIONING JEOL 3200FSC FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 3.0 1 FOURIER SPACE 15.0 FSC 0.143 CUT-OFF EMAN2 2.3 1321 EMAN2 2.3 emd_0530.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 140 140 140 -70 -70 -70 140 140 140 471.24 471.24 471.24 90.0 90.0 90.0 X Y Z -1.7491268 3.6039977 0.015334747 0.14911146 3.366 3.366 3.366 AUTHOR Averaged structure of AcrAB-TolC pump from E. coli cellular tomograms