<?xml version="1.0" encoding="UTF-8"?>
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    <admin>
        <current_status>
            <date>2024-03-20</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-02-06</deposition>
            <header_release>2019-04-24</header_release>
            <map_release>2019-05-29</map_release>
            <update>2024-03-20</update>
        </key_dates>
        <title>The structure of the photosystem I IsiA super-complex</title>
        <authors_list>
            <author>Toporik H</author>
            <author>Li J</author>
        </authors_list>
        <keywords>Photosystem, Antenna, Chlorophyll, Membrane, PHOTOSYNTHESIS</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Toporik H</author>
                    <author order="2">Li J</author>
                    <author order="3">Williams D</author>
                    <author order="4">Chiu PL</author>
                    <author order="5">Mazor Y</author>
                    <title>The structure of the stress-induced photosystem I-IsiA antenna supercomplex.</title>
                    <journal_abbreviation>Nat.Struct.Mol.Biol.</journal_abbreviation>
                    <country>US</country>
                    <volume>26</volume>
                    <first_page>443</first_page>
                    <last_page>449</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">31133699</external_references>
                    <external_references type="DOI">doi:10.1038/s41594-019-0228-8</external_references>
                    <external_references type="ISSN">1545-9985</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6nwa</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>PSI-IsiA</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>PSI-IsiA</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>8</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>10</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <details>PSI-IsiA from cyanobacteria</details>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">2</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Photosystem I P700 chlorophyll a apoprotein A1</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.083022375</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTISPPEREAKAKVSVDNNPVPTSFEKWGKPGHFDRTLARGPKTTTWIWNLHANAHDFDSQTSDLEDVSRKIFSAHFGHL
AVVFVWLSGMYFHGAKFSNYEGWLADPTHIKPSAQVVWPIVGQGILNGDVGGGFHGIQITSGLFYLWRASGFTDSYQLYC
TAIGGLVMAALMLFAGWFHYHVKAPKLEWFQNVESMMNHHLAGLLGLGSLGWAGHQIHVSMPINKLLDAGVAPKDIPLPH
EFILEPSKMAELYPSFAQGLTPFFTLNWGVYSDFLTFKGGLNPVTGGLWLSDTAHHHLAIAVLFIIAGHMYRTNWGIGHS
MKEILEAHKGPFTGEGHKGLYEILTTSWHAQLAINLALLGSLTIIVAQHMYAMPPYPYQAIDYATQLSLFTHHMWIGGFL
IVGAGAHGAIFMVRDYDPAKNVNNLLDRMLRHRDAIISHLNWVCIFLGFHSFGLYIHNDTMRALGRPQDMFSDTAIQLQP
IFAQWVQHLHTLAPGATAPNALATASYAFGGETIAVAGKVAMMPITLGTADFMVHHIHAFTIHVTALILLKGVLYARSSR
LVPDKANLGFRFPCDGPGRGGTCQVSGWDHVFLGLFWMYNSLSVVIFHFSWKMQSDVWGTVSPDGSVTHVTLGNFAQSAI
TINGWLRDFLWAQAANVINSYGSALSAYGIMFLAGHFVFAFSLMFLFSGRGYWQELIESIVWAHNKLNVAPAIQPRALSI
IQGRAVGVAHYLLGGIVTTWAFFLARSLSIG</string>
                    <external_references type="UNIPROTKB">P29254</external_references>
                </sequence>
                <ec_number>1.97.1.12</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Photosystem I P700 chlorophyll a apoprotein A2</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.081369531</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MATKFPKFSQDLAQDPTTRRIWYGIATAHDFETHDGMTEENLYQKIFASHFGHIAIIFLWTSGTLFHVAWQGNFEQWIKD
PLNIRPIAHAIWDPHFGEGAVNAFTQAGASNPVNIAYSGVYHWFYTIGMTTNQELYSGAVFLLVLASLFLFAGWLHLQPK
FRPSLAWFKNAESRLNHHLAGLFGVSSLAWAGHLVHVAIPEARGQHVGWDNFLSTPPHPAGLMPFFTGNWGVYAADPDTA
GHIFGTSEGAGTAILTFLGGFHPQTESLWLTDIAHHHLAIAVIFIIAGHMYRTNWGIGHSIKEILNAHKGPLTGAGHTNL
YDTINNSLHFQLGLALASLGVITSLVAQHMYSLPSYAFIAQDHTTQAALYTHHQYIAGFLMVGAFAHGAIFFVRDYDPVA
NKDNVLARMLEHKEALISHLSWVSLFLGFHTLGLYVHNDVVVAFGTPEKQILIEPVFAQWIQATSGKALYGFDVLLSNPD
SIASTTGAAWLPGWLDAINSGTNSLFLTIGPGDFLVHHAIALGLHTTALILIKGALDARGSKLMPDKKDFGYSFPCDGPG
RGGTCDISAWDAFYLAMFWMLNTLGWLTFYWHWKHLGVWSGNVAQFNENSTYLMGWFRDYLWANSAQLINGYNPYGVNNL
SVWAWMFLFGHLVWATGFMFLISWRGYWQELIETIVWAHERTPLANLVRWKDKPVALSIVQARLVGLAHFTVGYVLTYAA
FLIASTAGKFG</string>
                    <external_references type="UNIPROTKB">P29255</external_references>
                </sequence>
                <ec_number>1.97.1.12</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Photosystem I iron-sulfur center</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.008837261</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSHSVKIYDTCIGCTQCVRACPLDVLEMVPWDGCKAAQIASSPRTEDCVGCKRCETACPTDFLSIRVYLGAETTRSMGLA
Y</string>
                    <external_references type="UNIPROTKB">P32422</external_references>
                </sequence>
                <ec_number>1.97.1.12</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>Photosystem I reaction center subunit II</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.015663748999999998</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTELSGQPPKFGGSTGGLLSKANREEKYAITWTSASEQVFEMPTGGAAIMNEGENLLYLARKEQCLALGTQLRTKFKPKI
QDYKIYRVYPSGEVQYLHPADGVFPEKVNEGREAQGTKTRRIGQNPEPVTIKFSGKAPYEV</string>
                    <external_references type="UNIPROTKB">P19569</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="5">
                <name>Photosystem I reaction center subunit IV</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.008154086</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MALNRGDKVRIKRTESYWYGDVGTVASVEKSGILYPVIVRFDRVNYNGFSGSASGVNTNNFAENELELVQAAAK</string>
                    <external_references type="UNIPROTKB">P12975</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="6">
                <name>Photosystem I reaction center subunit III</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.018267081999999997</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MKHLLALLLAFTLWFNFAPSASADDFANLTPCSENPAYLAKSKNFLNTTNDPNSGKIRAERYASALCGPEGYPHLIVDGR
FTHAGDFLIPSILFLYIAGWIGWVGRSYLIEIRESKNPEMQEVVINVPLAIKKMLGGFLWPLAAVGEYTSGKLVMKDSEI
PTSPR</string>
                    <external_references type="UNIPROTKB">P29256</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="7">
                <name>Photosystem I reaction center subunit VIII</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.004414148</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MDGSYAASYLPWILIPMVGWLFPAVTMGLLFIHIESEGEG</string>
                    <external_references type="UNIPROTKB">Q55330</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="8">
                <name>Photosystem I reaction center subunit IX</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.004535415</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MDGLKSFLSTAPVMIMALLTFTAGILIEFNRFYPDLLFHP</string>
                    <external_references type="UNIPROTKB">Q55329</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="9">
                <name>Photosystem I reaction center subunit XI</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.016631794999999998</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAESNQVVQAYNGDPFVGHLSTPISDSAFTRTFIGNLPAYRKGLSPILRGLEVGMAHGYFLIGPWTLLGPLRDSEYQYIG
GLIGALALILVATAALSSYGLVTFQGEQGSGDTLQTADGWSQFAAGFFVGGMGGAFVAYFLLENLSVVDGIFRGLFN</string>
                    <external_references type="UNIPROTKB">P37277</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="10">
                <name>Photosystem I reaction center subunit XII</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.003382063</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MALSDTQILAALVVALLPAFLAFRLSTELYK</string>
                    <external_references type="UNIPROTKB">P72986</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="11">
                <name>Iron stress-induced chlorophyll-binding protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.037250733999999994</theoretical>
                </molecular_weight>
                <number_of_copies>18</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MQTYGNDTVQYEWWAGNARFADQSGLFIAAHVAQAALTAFWAGAFTLFEISRFDPTQAMGDQGLILLPHLATLGWGVGDG
GQIVDTYPYFVIGSIHLIASAVLGAGALFHTLRAPADLSTLKGQGKKFHFTWENPQQLGIILGHHLLFLGAGALLLAGKA
MYWGGLYDATTQTVRLVSQPTLDPLVIYGYQTHFASISSLEDLVGGHIFVGFLLIGGGIWHILVPPLGWAKKVLLFSGEA
ILSYSLGGIALAGFVAAYFCAVNTLAYPPEFYGPPLAIKLGIFPYFADTVELPMHAHTSRAWLANAHFFLAFFFLQGHLW
HALRALGFDFKRVEQAFDSLQT</string>
                    <external_references type="UNIPROTKB">Q55274</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="12">
                <name>Photosystem I reaction center subunit PsaK 1</name>
                <natural_source database="NCBI">
                    <organism ncbi="1148">Synechocystis sp. PCC 6803</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.008649268</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MHSFLLATAVPATLSWSPKVAGVMIACNILAIAFGKLTIKQQNVGTPMPSSNFFGGFGLGAVLGTASFGHILGAGVILGL
ANMGVL</string>
                    <external_references type="UNIPROTKB">P72712</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="13">
                <name>1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.00072297</theoretical>
                </molecular_weight>
                <number_of_copies>9</number_of_copies>
                <formula>LHG</formula>
            </ligand>
            <ligand macromolecule_id="14">
                <name>1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.0007871579999999999</theoretical>
                </molecular_weight>
                <number_of_copies>15</number_of_copies>
                <formula>LMG</formula>
            </ligand>
            <ligand macromolecule_id="15">
                <name>CHLOROPHYLL A ISOMER</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000893489</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <formula>CL0</formula>
            </ligand>
            <ligand macromolecule_id="16">
                <name>CHLOROPHYLL A</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000893489</theoretical>
                </molecular_weight>
                <number_of_copies>588</number_of_copies>
                <formula>CLA</formula>
            </ligand>
            <ligand macromolecule_id="17">
                <name>PHYLLOQUINONE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000450696</theoretical>
                </molecular_weight>
                <number_of_copies>6</number_of_copies>
                <formula>PQN</formula>
            </ligand>
            <ligand macromolecule_id="18">
                <name>IRON/SULFUR CLUSTER</name>
                <molecular_weight>
                    <theoretical units="MDa">0.00035163999999999996</theoretical>
                </molecular_weight>
                <number_of_copies>9</number_of_copies>
                <formula>SF4</formula>
            </ligand>
            <ligand macromolecule_id="19">
                <name>BETA-CAROTENE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000536873</theoretical>
                </molecular_weight>
                <number_of_copies>135</number_of_copies>
                <formula>BCR</formula>
            </ligand>
            <ligand macromolecule_id="20">
                <name>DODECYL-BETA-D-MALTOSIDE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000510615</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <formula>LMT</formula>
            </ligand>
            <ligand macromolecule_id="21">
                <name>CALCIUM ION</name>
                <molecular_weight>
                    <theoretical units="MDa">4.0078e-05</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <formula>CA</formula>
            </ligand>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">1.6</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>572000</number_selected>
                </particle_selection>
                <startup_model type_of_model="NONE"/>
                <final_reconstruction>
                    <number_classes_used>95</number_classes_used>
                    <applied_symmetry>
                        <point_group>C3</point_group>
                    </applied_symmetry>
                    <algorithm>BACK PROJECTION</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">3.48</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <processing_details>3</processing_details>
                        </software>
                    </software_list>
                    <number_images_used>74000</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3</version>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
                <final_three_d_classification>
                    <software_list>
                        <software>
                            <name>RELION</name>
                        </software>
                    </software_list>
                </final_three_d_classification>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="500001">
        <file>emd_0524.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>500</col>
            <row>500</row>
            <sec>500</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>500</x>
            <y>500</y>
            <z>500</z>
        </spacing>
        <cell>
            <a units="Å">512.0</a>
            <b units="Å">512.0</b>
            <c units="Å">512.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.049302652</minimum>
            <maximum>0.15141094</maximum>
            <average>0.00014315994</average>
            <std>0.0019637637</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.024</x>
            <y units="Å">1.024</y>
            <z units="Å">1.024</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.0125</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::D_1000239395::::</label>
        <annotation_details>PSI-IsiA super-complex from cyanobacteria</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>OTHER</refinement_protocol>
                <refinement_space>REAL</refinement_space>
                <overall_bvalue>16.43</overall_bvalue>
            </modelling>
        </modelling_list>
    </interpretation>
</emd>
