2024-03-20 REL RCSB RCSB RCSB 2019-01-31 2019-02-27 2019-03-13 2024-03-20 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) P41GM103832 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) U54GM103297 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) R01GM079429 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) S10OD021600 United States National Natural Science Foundation of China (NSFC) 31825008 China National Natural Science Foundation of China (NSFC) 31422014 China Small conformation of apo CRISPR_Csm complex Zhang K Pintilie G CRISPR, Type III-A, ssRNAase, ssDNase, HYDROLASE, TRANSFERASE-RNA complex Guo M Zhang K Zhu Y Pintilie GD Guan X Li S Schmid MF Ma Z Chiu W Huang Z Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry. Cell Res. CN 29 305 312 2019 30814678 doi:10.1038/s41422-019-0151-x 1748-7838 EMD-0516 other EM volume
Small conformation of ssRNA-bound CRISPR_Csm complex
 EMD-0517 other EM volume
Big conformation of ssRNA-bound CRISPR_Csm complex
 EMD-0518 other EM volume
Big conformation of apo CRISPR_Csm complex
 6nue FULLOVERLAP Small conformation of apo CRISPR_Csm complex Small conformation of apo CRISPR_Csm complex 0 1 2 3 4 5 Streptococcus thermophilus CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) Streptococcus thermophilus 0.086930672 1 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO MKKEKIDLFYGALLHDIGKVIQRATGERKKHALVGADWFDEIADNQVISDQIRYHMANYQSDKLGNDHLAYITYIADNIA SGVDRRQSNEESDEDASAKIWDTYTNQADIFNVFGAQTDKRYFKPTVLNLKSKPNFASATYEPFSKGDYAAIATRIKNEL AEFEFNQAQIDSLLNLFEAILSFVPSSTNSKEIADISLAEHSRLTAAFALAIYDYLEDKGRHNYKEDLFTKASAFYEEEA FLLASFDLSGIQDFIYNIATSGAAKQLKARSLYLDFMSEYIADSLLDKLGLNRANLLYVGGGHAYFVLANTEKTVETLVQ FEKDFNQFLLANFQTRLYVAFGWGSFAAKDIMSELNSPESYRQIYQKASRMISEKKISRYDYRTLMLLNRGGKSSERECE ICHSVENLVSYHDQKVCDICRGLYQFSKEIAHDHFIITENEGLPIGPNACLKGVAFEKLSQESFSRVYVKNDYKAGTIKA THVFVGDYQCDEIHKYAALSKNEDGLGIKRLAVVRLDVDDLGAAFMAGFSRQGNGQYSTLSRSATFSRSMSLFFKVYINQ FASDKKLSIIYAGGDDVFAIGSWQDIIAFTVELRQNFIKWTNGKLTLSAGIGLFADKTPISLMAHQTGELEEAAKGNEKD SISLFSSDYTFKFDRFITNVYDDKLEQIRYFFNHQDERGKNFIYKLIELLRNYESEEKMNVARLAYYLTRLEELTDKDER DKFKQFKKLFFKWYTNNESDRKEAELALLLYVYEIRKD A0A0A7HFE1 3.1.-.- CRISPR system Cms protein Csm2 Streptococcus thermophilus 0.014238391 3 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO MAILTDENYVDKAERAISLLEKDNKGNYLLTTSQIRKLLSLCSSLYDRSKERKFDELINDVSYLRVQFVYQAGREIAVKD LIEKAQILEALKEIKDRETLQRFCRYMEALVAYFKFYGGKD A0A0A7HIX1 CRISPR type III-associated RAMP protein Csm3 Streptococcus thermophilus 0.024600918 5 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO MTFAKIKFSAQIRLETGLHIGGSDAFAAIGAIDSPVIKDPITNIPIIPGSSLKGKMRTLLAKVYNEKVAEKPSDDSDILS RLFGNSKDKRFKMGRLIFRDAFLSNADELDSLGVRSYTEVKFENTIDRITAEANPRQIERAIRNSTFDFELIYEITDENE NQVEEDFKVIRDGLKLLELDYLGGSGSRGYGKVAFEKLKATTVFGNYDVKTLNELLTAEV A0A0A7HIF0 crRNA Streptococcus thermophilus 0.022935552999999997 1 ACGGAAACUUUCGUAACUGUUUAAUUCUGUUCACUUAUUCCACCGAUAUAAACCUAAUUACCUCGAGAGGGG CRISPR type III-associated RAMP protein Csm4 Streptococcus thermophilus 0.033786949 1 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO MTYKLYIMTFQNAHFGSGTLDSSKLTFSADRIFSALVLESLKMGKLDAFLAEANQDKFTLTDAFPFQFGPFLPKPIGYPK HDQIDQSVDVKEVRRQAKLSKKLQFLALENVDDYLNGELFENEEHAVIDTVTKNQPHKDGNLYQVATTRFSNDTSLYVIA NESDLLNELMSSLQYSGLGGKRSSGFGRFELDIQNIPLELSDRLTKNHSDKVMSLTTALPVDADLEEAMEDGHYLLTKSS GFAFSHATNENYRKQDLYKFASGSTFSKTFEGQIVDVRPLDFPHAVLNYAKPLFFKLEV A0A0A7HGA1 ADENOSINE-5'-TRIPHOSPHATE 0.000507181 1 ATP singleParticle particle 0.4 7.6
unspecified
 ETHANE 100 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 7.0 1 3.3 FSC 0.143 CUT-OFF cryoSPARC 0.65 81540 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0519.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 407.03998 407.03998 407.03998 90.0 90.0 90.0 X Y Z -2.724599 5.119803 -0.00011027274 0.1352678 1.06 1.06 1.06 0.8 AUTHOR Small conformation of apo CRISPR_Csm complex