<?xml version='1.0' encoding='utf-8'?>
<emd emdb_id="EMD-0517" version="3.0.1.3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_3/emdb.xsd">
    <admin>
        <current_status>
            <date>2019-04-17</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-01-31</deposition>
            <header_release>2019-02-27</header_release>
            <map_release>2019-03-13</map_release>
            <update>2019-04-17</update>
        </key_dates>
        <title>Big conformation of ssRNA-bound CRISPR_Csm complex</title>
        <authors_list>
            <author>Zhang K</author>
            <author>Pintilie G</author>
            <author>Li S</author>
            <author>Zhu Y</author>
            <author>Chiu W</author>
            <author>Huang Z</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Guo M</author>
                    <author order="2">Zhang K</author>
                    <author order="3">Zhu Y</author>
                    <author order="4">Pintilie GD</author>
                    <author order="5">Guan X</author>
                    <author order="6">Li S</author>
                    <author order="7">Schmid MF</author>
                    <author order="8">Ma Z</author>
                    <author order="9">Chiu W</author>
                    <author order="10">Huang Z</author>
                    <title>Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.</title>
                    <journal_abbreviation>Cell Res.</journal_abbreviation>
                    <country>CN</country>
                    <volume>29</volume>
                    <first_page>305</first_page>
                    <last_page>312</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">30814678</external_references>
                    <external_references type="DOI">doi:10.1038/s41422-019-0151-x</external_references>
                    <external_references type="ISSN">1748-7838</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-0517</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
                <details>Big conformation of ssRNA-bound CRISPR_Csm complex</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0516</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Small conformation of ssRNA-bound CRISPR_Csm complex</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0518</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Big conformation of apo CRISPR_Csm complex</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0519</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Small conformation of apo CRISPR_Csm complex</details>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>Big conformation of ssRNA-bound CRISPR_Csm complex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Big conformation of ssRNA-bound CRISPR_Csm complex</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="1308">Streptococcus thermophilus</organism>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="866768">Escherichia coli 'BL21-Gold(DE3)pLysS AG'</recombinant_organism>
                </recombinant_expression>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.4</concentration>
                    <buffer>
                        <ph>7.6</ph>
                    </buffer>
                    <grid>
                        <details>unspecified</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                        <details />
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">7.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <resolution res_type="BY AUTHOR" units="Å">3.4</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                            <version>0.65</version>
                        </software>
                    </software_list>
                    <number_images_used>73422</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="226493">
        <file>emd_0517.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>384</col>
            <row>384</row>
            <sec>384</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>384</x>
            <y>384</y>
            <z>384</z>
        </spacing>
        <cell>
            <a units="Å">407.03998</a>
            <b units="Å">407.03998</b>
            <c units="Å">407.03998</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-2.4075117</minimum>
            <maximum>5.0636206</maximum>
            <average>0.0015945727</average>
            <std>0.13433234</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.06</x>
            <y units="Å">1.06</y>
            <z units="Å">1.06</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.75</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::D_1000239462::::</label>
        <annotation_details>Big conformation of ssRNA-bound CRISPR_Csm complex</annotation_details>
    </map>
</emd>