Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)

2025-06-04 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name DERIVED_CALCULATIONS atom_site chem_comp_atom chem_comp_bond database_2 struct_conn struct_conn_type _atom_site.auth_atom_id _atom_site.label_atom_id _database_2.pdbx_DOI _database_2.pdbx_database_accession _struct_conn.conn_type_id _struct_conn.id _struct_conn.pdbx_dist_value _struct_conn.pdbx_leaving_atom_flag _struct_conn.ptnr1_auth_comp_id _struct_conn.ptnr1_auth_seq_id _struct_conn.ptnr1_label_atom_id _struct_conn.ptnr1_label_comp_id _struct_conn.ptnr1_label_seq_id _struct_conn.ptnr2_auth_comp_id _struct_conn.ptnr2_auth_seq_id _struct_conn.ptnr2_label_asym_id _struct_conn.ptnr2_label_atom_id _struct_conn.ptnr2_label_comp_id _struct_conn.ptnr2_label_seq_id _struct_conn_type.id STRUCTURE_SUMMARY em_admin pdbx_entry_details pdbx_modification_feature _em_admin.last_update _pdbx_entry_details.has_protein_modification DATA_COLLECTION em_admin em_software _em_admin.last_update _em_software.name RCSB RCSB 2019-01-30 2019-02-20 2020-05-06 2025-06-04 National Institutes of Health/National Cancer Institute (NIH/NCI) DP2CA186571 United States Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP) Huang W Taylor D Holoenzyme complex, Phosphatase, Activator, HYDROLASE-ACTIVATOR complex Leonard D Huang W Izadmehr S O'Connor CM Wiredja DD Wang Z Zaware N Chen Y Schlatzer DM Kiselar J Vasireddi N Schuchner S Perl AL Galsky MD Xu W Brautigan DL Ogris E Taylor DJ Narla G Selective PP2A Enhancement through Biased Heterotrimer Stabilization. Cell 181 688 701.e16 2020 32315618 doi:10.1016/j.cell.2020.03.038 1097-4172 6nts FULLOVERLAP PP2A Aalpha-B56alpha-Calpha holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP) PP2A Aalpha-B56alpha-Calpha holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP) 0 1 2 Homo sapiens Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Homo sapiens 0.06537834399999999 1 Escherichia coli LEVO MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLV GGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSS AVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDL EALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCREN VIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQL SQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHA TIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEV KPILEKLTQDQDVDVKYFAQEALTVLSLA P30153 Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform Homo sapiens 0.056266554999999996 1 Escherichia coli LEVO MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCI LFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQ LVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYET EHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPK TCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLY KISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSIL SNTSAE Q15172 Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Homo sapiens 0.0350215 1 Spodoptera frugiperda LEVO MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNY LFMGDYVNRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVD GQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRA HQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTYF(MLL) P67775 3.1.3.16 N-[(1R,2R,3S)-2-hydroxy-3-(10H-phenoxazin-10-yl)cyclohexyl]-4-(trifluoromethoxy)benzene-1-sulfonamide 0.0005205209999999999 1 L2J MANGANESE (II) ION 5.4938e-05 2 MN singleParticle particle 7.02

unspecified

ETHANE FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 40.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION

ab initio model generated by cryoSparc

C1 3.63 FSC 0.143 CUT-OFF 83784 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0510.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 357.504 357.504 357.504 90.0 90.0 90.0 X Y Z -0.18912628 1.3795784 -0.0009118488 0.032503888 1.064 1.064 1.064 0.3 AUTHOR ::::EMDATABANK.org::::EMD-0510:::: emd_0510_msk_1.map emd_0510_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 357.504 357.504 357.504 90.0 90.0 90.0 X Y Z -0.97515506 1.642666 0.0006642311 0.08312227 1.064 1.064 1.064 AUTHOR ::::EMDATABANK.org::::EMD-0510:::: emd_0510_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 357.504 357.504 357.504 90.0 90.0 90.0 X Y Z -0.87705684 1.9927876 0.0006814376 0.113026865 1.064 1.064 1.064 AUTHOR ::::EMDATABANK.org::::EMD-0510::::