2024-03-20 REL RCSB RCSB RCSB 2019-01-24 2019-02-06 2019-04-03 2024-03-20 National Institutes of Health/National Center for Research Resources (NIH/NCRR) R01GM071940/AI094386/DE025567 United States National Institutes of Health/National Center for Research Resources (NIH/NCRR) R01DK46917/DK53462/DK58333 United States CryoEM structure of Helicobacter pylori urea channel in closed state Cui YX Zhou K Helicobacter pylori, urea channel, closed state, TRANSPORT PROTEIN Cui Y Zhou K Strugatsky D Wen Y Sachs G Zhou ZH Munson K pH-dependent gating mechanism of theHelicobacter pyloriurea channel revealed by cryo-EM. Sci Adv US 5 eaav8423 eaav8423 2019 30906870 doi:10.1126/sciadv.aav8423 2375-2548 6nsj FULLOVERLAP urea channel of Helicobacter pylori urea channel of Helicobacter pylori 0 1 Helicobacter pylori J99 0.02 Acid-activated urea channel Helicobacter pylori (strain J99 / ATCC 700824) J99 / ATCC 700824 0.022533259999999996 6 Escherichia coli LEVO MLGLVLLYVGIVLISNGICGLTKVDPKSTAVMNFFVGGLSIVCNVVVITYSALHPTAPVEHHHHHHGAEDIVQVSHHLTS FYGPATGLLFGFTYLYAAINHTFGLDWRPYSWYSLFVAINTVPAAILSHYSDMLDDHKVLGITEGDWWAIIWLAWGVLWL TAFIENILKIPLGKFTPWLAIIEGILTAWIPAWLLFIQHWV P56874 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHATE 0.000591777 18 XP4 singleParticle twoDArray 2 7.0 10.0 BisTris 150.0 NaCl Quantifoil R2/1 COPPER 300 FORMVAR HOLEY GLOW DISCHARGE ETHANE 100 277.15 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 1.4000000000000001 1.2 2.4 2.6 130000.0 46730.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 98.0 100.0 GIF Quantum LS 20 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 3710 3838 2-99 1 4026 15.0 72.0 1 1221897 3 C6 FOURIER SPACE 2.7 FSC 0.143 CUT-OFF RELION 2.0 393930 MAXIMUM LIKELIHOOD RELION 2.0 MAXIMUM LIKELIHOOD RELION 2.0 3 RELION 2.0 emd_0498.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 171.20001 171.20001 171.20001 90.0 90.0 90.0 X Y Z -0.08286664 0.20162645 -0.00047695517 0.010737364 1.07 1.07 1.07 0.043 AUTHOR em-volume_P1 3UX4 PDB experimental model RIGID BODY FIT REAL 71.900000000000006 emd_0498_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 171.20001 171.20001 171.20001 90.0 90.0 90.0 X Y Z -0.011061661 0.04782223 -0.00023829403 0.0037193245 1.07 1.07 1.07 AUTHOR em-half-volume_P2 emd_0498_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 171.20001 171.20001 171.20001 90.0 90.0 90.0 X Y Z -0.010247017 0.047589753 -0.00023866114 0.0037232249 1.07 1.07 1.07 AUTHOR em-half-volume_P1