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    <admin>
        <current_status>
            <date>2024-03-20</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-01-24</deposition>
            <header_release>2019-02-06</header_release>
            <map_release>2019-09-11</map_release>
            <update>2024-03-20</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>R35GM122510</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-EM reconstruction of CFA/I pili</title>
        <authors_list>
            <author>Zheng W</author>
            <author>Andersson M</author>
        </authors_list>
        <keywords>Enterotoxigenic Escherichia coli (ETEC), CFA/I pili, helical reconstruction, PROTEIN FIBRIL</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Zheng W</author>
                    <author order="2">Andersson M</author>
                    <author order="3">Mortezaei N</author>
                    <author ORCID="0000-0003-2447-6209" order="4">Bullitt E</author>
                    <author ORCID="0000-0003-4844-5212" order="5">Egelman E</author>
                    <title>Cryo-EM structure of the CFA/I pilus rod.</title>
                    <journal_abbreviation>Iucrj</journal_abbreviation>
                    <country>UK</country>
                    <volume>6</volume>
                    <first_page>815</first_page>
                    <last_page>821</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">31576215</external_references>
                    <external_references type="DOI">doi:10.1107/S2052252519007966</external_references>
                    <external_references type="ISSN">2052-2525</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6nrv</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Enterotoxigenic Escherichia coli (ETEC) CFA/I pili</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Enterotoxigenic Escherichia coli (ETEC) CFA/I pili</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="316401">Escherichia coli ETEC H10407</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>CFA/I fimbrial subunit B</name>
                <natural_source database="NCBI">
                    <organism ncbi="562">ESCHERICHIA COLI ETEC O78:H12</organism>
                    <strain>E7473</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.014968839</theoretical>
                </molecular_weight>
                <number_of_copies>13</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>VEKNITVTASVDPAIDLLQADGNALPSAVKLAYSPASKTFESYRVMTQVHTNDATKKVIVKLADTPQLTDVLNSTVQMPI
SVSWGGQVLSTTAKEFEAAALGYSASGVNGVSSSQELVISAAPKTAGTAPTAGNYSGVVSLVMTLG</string>
                    <external_references type="UNIPROTKB">P0CK93</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>helical</method>
            <aggregation_state>filament</aggregation_state>
            <specimen_preparation_list>
                <helical_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                    </vitrification>
                </helical_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <helical_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON II (4k x 4k)</film_or_detector_model>
                            <detector_mode>INTEGRATING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">20.0</average_electron_dose_per_image>
                        </image_recording>
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                <image_recording_id>1</image_recording_id>
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                    <applied_symmetry>
                        <helical_parameters>
                            <delta_z units="Å">8.6</delta_z>
                            <delta_phi units="deg">113.3</delta_phi>
                            <axial_symmetry>C1</axial_symmetry>
                        </helical_parameters>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">4.0</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>SPIDER</name>
                        </software>
                    </software_list>
                    <number_images_used>97295</number_images_used>
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                <segment_selection>
                    <number_selected>117011</number_selected>
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                        <software>
                            <name>EMAN2</name>
                        </software>
                    </software_list>
                </segment_selection>
                <startup_model type_of_model="NONE">
                    <details>Featureless cylinder</details>
                </startup_model>
                <final_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </final_angle_assignment>
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        <file>emd_0497.map.gz</file>
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            <b units="Å">139.52</b>
            <c units="Å">139.52</c>
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            <beta units="deg">90.0</beta>
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