Cryo-EM structure of human TPC2 channel in the ligand-bound closed state

2025-06-04 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name STRUCTURE_SUMMARY chem_comp_atom chem_comp_bond database_2 em_admin pdbx_entry_details pdbx_modification_feature _database_2.pdbx_DOI _database_2.pdbx_database_accession _em_admin.last_update DATA_COLLECTION em_admin em_software _em_admin.last_update _em_software.name RCSB RCSB 2019-01-18 2019-03-06 2019-03-27 2025-06-04 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM079179 United States Howard Hughes Medical Institute (HHMI) United States Welch Foundation Grant I-1578 Cancer Prevention and Research Institute of Texas (CPRIT) United States Cryo-EM structure of human TPC2 channel in the ligand-bound closed state She J Zeng W channel, lysosome, TRANSPORT PROTEIN She J Zeng W Guo J Chen Q Bai X Jiang Y Structural mechanisms of phospholipid activation of the human TPC2 channel. Elife US 8 2019 30860481 doi:10.7554/eLife.45222 2050-084X EMD-0477 other EM volume EMD-0478 other EM volume 6nq2 FULLOVERLAP The complex of two-pore channel 2 with PI(3,5)P2 The complex of two-pore channel 2 with PI(3,5)P2 0 1 Homo sapiens Two pore calcium channel protein 2 Homo sapiens 0.08567182799999999 2 Homo sapiens LEVO GSLEMAEPQAESEPAAGGARGGGGDWPAGLTTYRSIQVGPGAAARWDLCIDQAVVFIEDAIQYRSINHRVDASSMWLYRR YYSNVCQRTLSFTIFLILFLAFIETPSSLTSTADVRYRAAPWEPPCGLTESVEVLCLLVFAADLSVKGYLFGWAHFQKNL WLLGYLVVLVVSLVDWTVSLSLVCHEPLRIRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMASVGLLLAIHLCLFTMFGML LFAGGKQDDGQDRERLTYFQNLPESLTSLLVLLTTANNPDVMIPAYSKNRAYAIFFIVFTVIGSLFLMNLLTAIIYSQFR GYLMKSLQTSLFRRRLGTRAAFEVLSSMVGEGGAFPQAVGVKPQNLLQVLQKVQLDSSHKQAMMEKVRSYGSVLLSAEEF QKLFNELDRSVVKEHPPRPEYQSPFLQSAQFLFGHYYFDYLGNLIALANLVSICVFLVLDADVLPAERDDFILGILNCVF IVYYLLEMLLKVFALGLRGYLSYPSNVFDGLLTVVLLVLEISTLAVYRLPHPGWRPEMVGLLSLWDMTRMLNMLIVFRFL RIIPSMKPMAVVASTVLGLVQNMRAFGGILVVVYYVFAIIGINLFRGVIVALPGNSSLAPANGSAPCGSFEQLEYWANNF DDFAAALVTLWNLMVVNNWQVFLDAYRRYSGPWSKIYFVLWWLVSSVIWVNLFLALILENFLHKWDPRSHLQPLAGTPEA TYQMTVELLFRDILEEPEEDELTERLSQHPHLWLCR Q8NHX9 (2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate 0.000746566 2 EUJ singleParticle particle 8.0

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ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 1.6 1 NONE 3.4 FSC 0.143 CUT-OFF 56548 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0479.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 256.80002 256.80002 256.80002 90.0 90.0 90.0 X Y Z -0.13506703 0.23433577 -0.000010255095 0.008334627 1.07 1.07 1.07 0.05 AUTHOR ::::EMDATABANK.org::::EMD-0479:::: Human TPC2 channel in the ligand-bound closed state