2025-12-10 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY STRUCTURE_SUMMARY em_admin em_entity_assembly em_imaging em_software entity entity_poly entity_src_gen struct_ref struct_ref_seq struct_ref_seq_dif _em_admin.last_update _em_entity_assembly.entity_id_list _em_imaging.microscope_model _em_software.category _em_software.fitting_id _em_software.image_processing_id _em_software.imaging_id _em_software.name _entity.formula_weight _entity.pdbx_description _entity.pdbx_ec _entity.pdbx_mutation _entity_poly.pdbx_seq_one_letter_code _entity_poly.pdbx_seq_one_letter_code_can _entity_poly.pdbx_strand_id _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name _entity_src_gen.pdbx_beg_seq_num _entity_src_gen.pdbx_end_seq_num _entity_src_gen.pdbx_gene_src_gene _entity_src_gen.pdbx_host_org_ncbi_taxonomy_id _entity_src_gen.pdbx_host_org_scientific_name _entity_src_gen.pdbx_src_id _struct_ref.db_code _struct_ref.entity_id _struct_ref.pdbx_align_begin _struct_ref.pdbx_db_accession _struct_ref.pdbx_seq_one_letter_code _struct_ref_seq.db_align_beg _struct_ref_seq.db_align_end _struct_ref_seq.pdbx_auth_seq_align_beg _struct_ref_seq.pdbx_auth_seq_align_end _struct_ref_seq.pdbx_db_accession _struct_ref_seq.pdbx_strand_id _struct_ref_seq.seq_align_beg _struct_ref_seq.seq_align_end _struct_ref_seq_dif.align_id _struct_ref_seq_dif.db_mon_id _struct_ref_seq_dif.details _struct_ref_seq_dif.mon_id _struct_ref_seq_dif.pdbx_auth_seq_num _struct_ref_seq_dif.pdbx_pdb_strand_id _struct_ref_seq_dif.pdbx_seq_db_accession_code _struct_ref_seq_dif.pdbx_seq_db_seq_num _struct_ref_seq_dif.seq_num DATABASE_REFERENCES citation _citation.journal_volume _citation.page_first _citation.page_last STRUCTURE_SUMMARY atom_site entity entity_name_com entity_poly entity_poly_seq entity_src_gen pdbx_poly_seq_scheme pdbx_struct_sheet_hbond pdbx_unobs_or_zero_occ_atoms pdbx_unobs_or_zero_occ_residues struct_conf struct_ref struct_ref_seq struct_ref_seq_dif struct_sheet_range _atom_site.label_seq_id _entity.formula_weight _entity.pdbx_description _entity.pdbx_mutation _entity_name_com.name _entity_poly.pdbx_seq_one_letter_code _entity_poly.pdbx_seq_one_letter_code_can _pdbx_struct_sheet_hbond.range_1_label_seq_id _pdbx_struct_sheet_hbond.range_2_label_seq_id _pdbx_unobs_or_zero_occ_atoms.label_seq_id _struct_conf.beg_label_seq_id _struct_conf.end_label_seq_id _struct_sheet_range.beg_label_seq_id _struct_sheet_range.end_label_seq_id AUTHOR_SUPPORTING_EVIDENCE pdbx_audit_support _pdbx_audit_support.funding_organization DATABASE_REFERENCES chem_comp_atom chem_comp_bond database_2 _database_2.pdbx_DOI _database_2.pdbx_database_accession COORDINATE_REPLACEMENT AUTHOR Model completeness STRUCTURE_SUMMARY atom_site em_admin em_entity_assembly em_imaging em_software entity entity_name_com entity_poly entity_poly_seq entity_src_gen pdbx_contact_author pdbx_entity_instance_feature pdbx_entry_details pdbx_nonpoly_scheme pdbx_poly_seq_scheme pdbx_struct_assembly pdbx_struct_oper_list pdbx_struct_sheet_hbond pdbx_unobs_or_zero_occ_atoms pdbx_unobs_or_zero_occ_residues pdbx_validate_close_contact pdbx_validate_rmsd_angle pdbx_validate_rmsd_bond pdbx_validate_torsion refine refine_ls_restr software struct_conf struct_ref struct_ref_seq struct_ref_seq_dif struct_sheet struct_sheet_order struct_sheet_range struct_site struct_site_gen _em_admin.last_update _em_entity_assembly.entity_id_list _em_imaging.microscope_model _em_software.category _em_software.fitting_id _em_software.image_processing_id _em_software.imaging_id _em_software.name _entity.formula_weight _entity.pdbx_description _entity.pdbx_ec _entity.pdbx_mutation _entity_name_com.name _entity_poly.pdbx_seq_one_letter_code _entity_poly.pdbx_seq_one_letter_code_can _entity_poly.pdbx_strand_id _entity_poly_seq.entity_id _entity_poly_seq.mon_id _entity_poly_seq.num _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name _entity_src_gen.pdbx_beg_seq_num _entity_src_gen.pdbx_end_seq_num _entity_src_gen.pdbx_gene_src_gene _entity_src_gen.pdbx_host_org_ncbi_taxonomy_id _entity_src_gen.pdbx_host_org_scientific_name _entity_src_gen.pdbx_src_id _pdbx_entry_details.has_ligand_of_interest _pdbx_entry_details.has_protein_modification _pdbx_nonpoly_scheme.auth_seq_num _pdbx_poly_seq_scheme.asym_id _pdbx_poly_seq_scheme.auth_mon_id _pdbx_poly_seq_scheme.auth_seq_num _pdbx_poly_seq_scheme.entity_id _pdbx_poly_seq_scheme.mon_id _pdbx_poly_seq_scheme.ndb_seq_num _pdbx_poly_seq_scheme.pdb_mon_id _pdbx_poly_seq_scheme.pdb_seq_num _pdbx_poly_seq_scheme.pdb_strand_id _pdbx_poly_seq_scheme.seq_id _pdbx_struct_assembly.details _pdbx_struct_oper_list.symmetry_operation _struct_conf.beg_auth_asym_id _struct_conf.beg_auth_comp_id _struct_conf.beg_auth_seq_id _struct_conf.beg_label_asym_id _struct_conf.beg_label_comp_id _struct_conf.beg_label_seq_id _struct_conf.end_auth_asym_id _struct_conf.end_auth_comp_id _struct_conf.end_auth_seq_id _struct_conf.end_label_asym_id _struct_conf.end_label_comp_id _struct_conf.end_label_seq_id _struct_conf.pdbx_PDB_helix_class _struct_conf.pdbx_PDB_helix_length _struct_ref.db_code _struct_ref.entity_id _struct_ref.pdbx_align_begin _struct_ref.pdbx_db_accession _struct_ref.pdbx_seq_one_letter_code _struct_ref_seq.db_align_beg _struct_ref_seq.db_align_end _struct_ref_seq.pdbx_auth_seq_align_beg _struct_ref_seq.pdbx_auth_seq_align_end _struct_ref_seq.pdbx_db_accession _struct_ref_seq.pdbx_strand_id _struct_ref_seq.seq_align_beg _struct_ref_seq.seq_align_end _struct_ref_seq_dif.align_id _struct_ref_seq_dif.db_mon_id _struct_ref_seq_dif.details _struct_ref_seq_dif.mon_id _struct_ref_seq_dif.pdbx_auth_seq_num _struct_ref_seq_dif.pdbx_pdb_strand_id _struct_ref_seq_dif.pdbx_seq_db_accession_code _struct_ref_seq_dif.pdbx_seq_db_seq_num _struct_ref_seq_dif.seq_num RCSB RCSB 2019-01-18 2019-06-19 2019-06-19 2025-12-10 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) 1R01 Al124491 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) 1DP1 HD087988 United States Sharif H Wang L Wu H Inflammasome, Activator, Biological process Immunity, Inflammatory response, Innate immunity, Transcription, Transcription regulation Ligand, ATP binding, Nucleotide binding, IMMUNE SYSTEM Sharif H Wang L Wang WL Magupalli VG Andreeva L Qiao Q Hauenstein AV Wu Z Nunez G Mao Y Wu H Nature UK 570 338 343 2019 31189953 doi:10.1038/s41586-019-1295-z 1476-4687 0006 NATUAS 6npy FULLOVERLAP EMDB EMD-0476 associated EM volume

HS01

NL3-NEK7 NL3-NEK7 0 1 NLRP3 1 2

The sequence is according to NP_001230062.1 isoform which lacks 2 residues from N-terminus. Total residues are 1034aa as compared to 1036 in UNP Q96P20. There are mutations in YRKKYRKY instead its IYCAKYRAY mutations were intended to introduce so that the class of MBP and NLRP3 is avoided

Homo sapiens NEK7 1 1 Homo sapiens Interferon-induced, double-stranded RNA-activated protein kinase,Serine/threonine-protein kinase Nek7 Homo sapiens 0.03201515 1 Escherichia coli LEVO GSTDKRFGMDFRIEKKIGRGQFSEVYRAACRLDGKTVALKKVQIFDLMDAKARADCIKEIDLLAQLNHPNVIKYYVCFIT GNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLG RFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHY SEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS P19525 Q8TDX7 2.7.11.1 Isoform 2 of NACHT, LRR and PYD domains-containing protein 3 Homo sapiens 0.11789098399999999 1 Spodoptera frugiperda LEVO MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRD LYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKIYCAKYRAYVRSRFQCIEDRNARLGESV SLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLD WASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDW QKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQEN EVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKI LFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLL ENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDF VQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVN SHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVE LDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNP QCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLS TLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW Q96P20 3.6.4.- ADENOSINE-5'-DIPHOSPHATE 0.000427201 1 ADP singleParticle particle 7.5 Quantifoil R1.2/1.3 COPPER 400 CARBON HOLEY GLOW DISCHARGE 60 ETHANE 100 277.1 FEI VITROBOT MARK II TFS TITAN THEMIS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 -1.0 -3.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 55.0 1 Gctf PHASE FLIPPING AND AMPLITUDE CORRECTION C1 3.8 FSC 0.143 CUT-OFF RELION 108771 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION RIGID BODY FIT REAL