2019-05-01 REL RCSB RCSB RCSB 2019-01-15 2019-01-30 2019-05-01 2019-05-01 HIV-1 Env State 2A Alsahafi N Bakouche N Kazemi M Richard J Ding S Bhattacharyya S Das D Anand SP Prevost J Tolbert WD Lu H Medjahed H Delgado GGO Kirk S Melillo B Mothes W Sodroski J Smith III AB Kaufmann DE Wu X Pazgier M Rouiller I Finzi A Munro JB Alsahafi N Bakouche N Kazemi M Richard J Ding S Bhattacharyya S Das D Anand SP Prevost J Tolbert WD Lu H Medjahed H Gendron-Lepage G Ortega Delgado GG Kirk S Melillo B Mothes W Sodroski J Kaufmann DE Wu X Pazgier M Rouiller I Finzi A Munro JB An Asymmetric Opening of HIV-1 Envelope Mediates Antibody-Dependent Cellular Cytotoxicity. Cell Host Microbe US 25 578 587.e5 2019 30974085 doi:10.1016/j.chom.2019.03.002 1934-6069 EMD-0466 associated EM volume HIV-1 Env in complex with BNM-III-170, A32 and 17b antibodies HIV-1 Env in complex with BNM-III-170, A32 and 17b antibodies 0
HIV-1 Env present on the surface of Aldrithiol-2 (AT-2)-inactivated HIV-1 particles incubated with BNM-III-170, A32 and 17b antibodies.
Human immunodeficiency virus 1 HIV-1 BAL/SuPT1-CCR5 CL.30 Human immunodeficiency virus 1 HIV-1 BAL/SuPT1-CCR5 CL.30 singleParticle particle 0.335 7.2 0.01 Tris-HCl 0.1 NaCl 1.0 EDTA Quantifoil R2/2 COPPER CARBON HOLEY 2000.0 GLOW DISCHARGE ETHANE 100 277.15 FEI VITROBOT MARK IV
Lot#P4311
 FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.7 1.1559 3.2076 79000. NITROGEN GATAN K2 SUMMIT (4k x 4k) 3838 3710 1033 1.4
1033 was the number of initial images (movies) that 1032 were selected for particle picking
 1
Collected movies aligned using motioncorr protocol
14076
The initial number of extracted particles (200*200)
 CTFFIND 4 RELION 2
Using CTFFind4 to estimate the CTF and corrected for CTF in refinement with Relion-auto-refine
The initial model obtained firstly by Relion-initial-map protocol and 3000 particles. Then using 4000 particles a more accurate map achieved by "highres protocol" (Scipion).
 1 C1 FOURIER SPACE 13.08 FSC 0.143 CUT-OFF RELION 2
The resolution obtained using gold-standard method by Relion-auto-refine
10597 MAXIMUM LIKELIHOOD RELION 2
Initial angular assignment achieved by Relion 2D classification and alignment protocol.
 MAXIMUM LIKELIHOOD RELION 2
After processing the data by Relion-auto-refine
 emd_0466.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 340.0 340.0 340.0 90.0 90.0 90.0 X Y Z -0.6560266 0.623866 -0.00458397 0.022168187 1.7 1.7 1.7 0.0246 AUTHOR Envelope