2024-03-20 REL RCSB RCSB RCSB 2019-01-14 2019-02-06 2019-02-13 2024-03-20 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) T32GM008570 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) ZIC ES103326 United States Anderson CJ Baird MR chromatin, structural biology, single particle, cryo-EM, histone methyltransferase, nucleosome, Dot1L, GENE REGULATION Anderson CJ Baird MR Hsu A Barbour EH Koyama Y Borgnia MJ McGinty RK Cell Rep US 26 1681 1690.e5 2019 30759380 doi:10.1016/j.celrep.2019.01.058 2211-1247 EMD-0459 other EM volume EMD-0460 other EM volume 6nn6 FULLOVERLAP Dot1L-H2BK120ub nucleosome complex (Class 2A) Dot1L-H2BK120ub nucleosome complex (Class 2A) 0 1 2 3 4 5 6 7 8 Homo sapiens 0.27 Histone H3.2 Xenopus laevis 0.01530393 2 Escherichia coli LEVO ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKT DLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA P84233 Histone H4 Xenopus laevis 0.011263230999999999 2 Escherichia coli LEVO SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKT VTAMDVVYALKRQGRTLYGFGG P62799 Histone H2A type 1 Xenopus laevis 0.013978240999999999 2 Escherichia coli LEVO SGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIP RHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK P06897 Histone H2B 1.1 Xenopus laevis 0.013642845999999998 2 Escherichia coli LEVO GSAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLA HYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTCYTSAK P02281 DNA (145-MER) synthetic construct 0.044520383 1 (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DA) (DT)(DC)(DG)(DA)(DT) DNA DNA (145-MER) synthetic construct 0.04499166 1 (DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA) (DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG) (DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA) (DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC) (DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG) (DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA) (DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT) (DC)(DT)(DG)(DA)(DT) DNA Histone-lysine N-methyltransferase, H3 lysine-79 specific Homo sapiens 0.047462039 1 Escherichia coli LEVO GSEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNR AIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFV DLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFV NNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRT ILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLN KKGRKMAGRKRGRPKK Q8TEK3 2.1.1.43 Ubiquitin Homo sapiens 0.00891118 1 Escherichia coli LEVO GSGSMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGC F5H388 singleParticle particle 0.93 7.5 Quantifoil R1.2/1.3 COPPER 300 PLASMA CLEANING 60 ETHANE 100 277.15 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) COUNTING 1 1000 60.0 48.0 1 408526 6ESF

low-pass filtered

1 C1 3.9 FSC 0.143 CUT-OFF RELION 2.1 39558 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 4 39558.0 RELION 2.1 1NW3 A 1-332 PDB experimental model 1UBQ A 1-74 PDB experimental model 3LZ0 A PDB experimental model 3LZ0 B PDB experimental model 3LZ0 C PDB experimental model 3LZ0 D PDB experimental model 3LZ0 E PDB experimental model 3LZ0 F PDB experimental model 3LZ0 G PDB experimental model 3LZ0 H PDB experimental model 3LZ0 I PDB experimental model 3LZ0 J PDB experimental model RIGID BODY FIT REAL