2023-09-06 REL RCSB RCSB RCSB 2019-01-12 2019-02-20 2019-07-10 2023-09-06 Howard Hughes Medical Institute (HHMI) United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) P01GM051487 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) F32GM122334 United States Life Sciences Research Foundation United States Negative stain EM structure of T. thermophilus Csm with a transcription elongation complex Liu J-J Liu TY Csm, RNA substrate, DNA, RNA BINDING PROTEIN, RNA polymerase Liu TY Liu J-J Aditham AJ Nogales E Doudna JA Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble Nat Commun UK 2019 doi:10.1038/s41467-019-10780-2 2041-1723 Complex of Thermus thermophilus Csm with the Thermus thermophilus transcription elongation complex Complex of Thermus thermophilus Csm with the Thermus thermophilus transcription elongation complex 0
T. thermophilus Csm was recombinantly expressed in E. coli, and T. thermophilus RNA polymerase was isolated from the natural source.
Thermus thermophilus singleParticle particle 7.9 NEGATIVE UA ETHANE FEI TECNAI 12 FLOOD BEAM BRIGHT FIELD LAB6 120 FEI CETA (4k x 4k) 58.0 1 17.0 FSC 0.5 CUT-OFF 8351 ANGULAR RECONSTITUTION MAXIMUM LIKELIHOOD emd_0455.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 -64 -64 -64 128 128 128 558.08 558.08 558.08 90.0 90.0 90.0 X Y Z -0.03912864 0.112419434 0.0001628129 0.006225997 4.36 4.36 4.36 0.03 AUTHOR Csm-RNAP