2019-05-01 REL RCSB RCSB RCSB 2018-12-13 2019-01-16 2019-02-13 2019-05-01 Type III-A CRISPR Effector Subcomplex from Staphylococcus epidermidis RP62a Dorsey BW Mondragon A Dorsey BW Huang L Mondragon A Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM. Nucleic Acids Res. UK 47 3765 3783 2019 30759237 doi:10.1093/nar/gkz079 1362-4962 0389 NARHAD EMD-0442 associated EM volume Type III-A CRISPR Effector Subcomplex from Staphylococcus epidermidis RP62a Type III-A CRISPR Effector Subcomplex from Staphylococcus epidermidis RP62a 0 1 2 3 4 5 Staphylococcus epidermidis RP62A 0.255 singleParticle particle 0.20 8.0 20.0 C4H11NO3 Tris 500.0 NaCl Sodium chloride C-flat-1.2/1.3 4C COPPER 400 GLOW DISCHARGE
Glow discharged with 15mA.
 ETHANE 99 277 FEI VITROBOT MARK IV
Blot for 4 seconds with +5 force before plunging..
JEOL 3200FS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.0 2.0 3.5 30000. 40323. GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER NITROGEN 100.0 100.0 In-column Omega Filter 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 3710 3838 5.0 1-40 7 1037 8.0 37.5 1 277413 CTFFIND 4.1 1 C1 FOURIER SPACE 5.2 FSC 0.143 CUT-OFF RELION 3.0 107620 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 4 RELION 3.0 emd_0442.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 244 244 244 0 0 0 244 244 244 302.56 302.56 302.56 90.0 90.0 90.0 X Y Z -0.027626785 0.0711628 0.00000088811134 0.0019536428 1.24 1.24 1.24 0.0185 AUTHOR Type III-A CRISPR Effector Subcomplex from Staphylococcus epidermidis RP62a, primary map