2024-10-30 REL RCSB RCSB RCSB 2018-12-07 2018-12-19 2019-04-17 2024-10-30 National Science Foundation (NSF, China) Shanghai 18ZR1447800 China National Natural Science Foundation of China (NSFC) 31300245 China National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM127710 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) DK102495, DK111427, DK116780 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 008424 United States Zhao L-H Ma S Parathyroid hormone receptor, GPCR, Class B GPCR, GPCR-G protein complex, SIGNALING PROTEIN Zhao LH Ma S Sutkeviciute I Shen DD Zhou XE de Waal PW Li CY Kang Y Clark LJ Jean-Alphonse FG White AD Yang D Dai A Cai X Chen J Li C Jiang Y Watanabe T Gardella TJ Melcher K Wang MW Vilardaga JP Xu HE Zhang Y Science US 364 148 153 2019 30975883 doi:10.1126/science.aav7942 1095-9203 0038 SCIEAS 6nbf FULLOVERLAP Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein 0 1 2 3 4 5 6 Homo sapiens Parathyroid hormone/parathyroid hormone-related peptide receptor Homo sapiens 0.054755003999999996 1 Spodoptera aff. frugiperda 2 RZ-2014 LEVO DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGR PCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDR LAMIYTVGYSVSLASLTVAVLILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQ APPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTVFGWGLPAVFVAVWVSVRATL ANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMA TPYTEVSGTLWQVQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGPMVSHEF Q03431 Long-acting parathyroid hormone analog Homo sapiens 0.004274027 1 LEVO AVAEIQLMHQRAKWIQDARRRAFLHKLIAEIHTAEI Gs protein alpha subunit Bos taurus 0.043867762 1 Spodoptera aff. frugiperda 2 RZ-2014 LEVO MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGYSEEECKQYKAVV YSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAA YYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMV IREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTR AKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Rattus norvegicus 0.037915496 1 Spodoptera aff. frugiperda 2 RZ-2014 LEVO MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQ DGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDN QIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRV SCLGVTDDGMAVATGSWDSFLKIWN P54311 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Bos taurus 0.007861143 1 Spodoptera aff. frugiperda 2 RZ-2014 LEVO MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL P63212 Nanobody-35 synthetic construct 0.013711283999999999 1 Escherichia coli LEVO QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLY LQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTV CHOLESTEROL 0.000386654 6 CLR PALMITIC ACID 0.00025642399999999994 4 PLM singleParticle particle 0.01 7.4 ETHANE 100 295 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 BASE (4k x 4k) 60.0 1 6B3J 3.0 FSC 0.143 CUT-OFF 211618 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RIGID BODY FIT