<?xml version='1.0' encoding='utf-8'?>
<emd emdb_id="EMD-0398" version="3.0.1.3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_3/emdb.xsd">
    <admin>
        <current_status>
            <date>2019-02-13</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2018-12-06</deposition>
            <header_release>2018-12-19</header_release>
            <map_release>2018-12-19</map_release>
            <update>2019-02-13</update>
        </key_dates>
        <title>Electron microscopy snapshots of single particles from single cells</title>
        <authors_list>
            <author>Yi X</author>
            <author>Verbeke EJ</author>
            <author>Yiran C</author>
            <author>Dickinson DJ</author>
            <author>Taylor DW</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Yi X</author>
                    <author order="2">Verbeke EJ</author>
                    <author order="3">Chang Y</author>
                    <author order="4">Dickinson DJ</author>
                    <author order="5">Taylor DW</author>
                    <title>Electron microscopy snapshots of single particles from single cells.</title>
                    <journal_abbreviation>J. Biol. Chem.</journal_abbreviation>
                    <country>US</country>
                    <volume>294</volume>
                    <first_page>1602</first_page>
                    <last_page>1608</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">30541924</external_references>
                    <external_references type="DOI">doi:10.1074/jbc.RA118.006686</external_references>
                    <external_references type="ISSN">1083-351X</external_references>
                    <external_references type="CSD">0071</external_references>
                    <external_references type="ASTM">JBCHA3</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-0398</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0400</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0399</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>40S ribosome from single cells</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>40S ribosome from single cells</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="6239">Caenorhabditis elegans</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">1</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.02</concentration>
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <staining>
                        <type>NEGATIVE</type>
                        <material>Uranyl Acetate</material>
                    </staining>
                    <grid>
                        <details>unspecified</details>
                    </grid>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>JEOL 2010F</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN MULTISCAN</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">30.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>80000</number_selected>
                </particle_selection>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution res_type="BY AUTHOR" units="Å">48.0</resolution>
                    <resolution_method>FSC 0.5 CUT-OFF</resolution_method>
                    <number_images_used>1500</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing />
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing />
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="16385">
        <file>emd_0398.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>160</col>
            <row>160</row>
            <sec>160</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>160</x>
            <y>160</y>
            <z>160</z>
        </spacing>
        <cell>
            <a units="Å">576.0</a>
            <b units="Å">576.0</b>
            <c units="Å">576.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.04331638</minimum>
            <maximum>0.08605843</maximum>
            <average>0.00016707607</average>
            <std>0.0047171037</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">3.6</x>
            <y units="Å">3.6</y>
            <z units="Å">3.6</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.0235</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0398::::</label>
        <annotation_details>Reconstruction of 40S ribosome</annotation_details>
    </map>
</emd>