2019-03-06 REL RCSB RCSB RCSB 2018-12-05 2019-02-20 2019-03-06 2019-03-06 Gao Y Fox T Val N Yang W Gao Y Cui Y Fox T Lin S Wang H de Val N Zhou ZH Yang W Science US 363 2019 30679383 doi:10.1126/science.aav7003 1095-9203 0038 SCIEAS EMD-0390 associated EM volume EMD-0364 other EM volume EMD-0365 other EM volume EMD-0379 other EM volume EMD-0380 other EM volume EMD-0381 other EM volume EMD-0382 other EM volume EMD-0386 other EM volume EMD-0387 other EM volume EMD-0388 other EM volume EMD-0389 other EM volume EMD-0391 other EM volume EMD-0392 other EM volume EMD-0393 other EM volume EMD-0394 other EM volume EMD-0395 other EM volume T7 DNA polymerase/Trx interacting with two primase domains (A and B) of T7 helicase (gp4) in complex with a fork DNA substrate and dTTP (from gp4-gp5 lagging-strand complex LagL4) T7 DNA polymerase/Trx interacting with two primase domains (A and B) of T7 helicase (gp4) in complex with a fork DNA substrate and dTTP (from gp4-gp5 lagging-strand complex LagL4) 0 1 2 3 4 5 Enterobacteria phage T7 Escherichia coli gene product 4 helicase-primase Enterobacteria phage T7 LEVO MDNSHDSDSVFLYHIPCDNCGSSDGNSLFSDGHTFCYVCEKWTAGNEDTKERASKRKPSG GKPMTYNVWNFGESNGRYSALTARGISKETCQKAGYWIAKVDGVMYQVADYRDQNGNIVS QKVRDKDKNFKTTGSHKSDALFGKHLWNGGKKIVVTEGEIDMLTVMELQDCKYPVVSLGH GASAAKKTCAANYEYFDQFEQIILMFDMDEAGRKAVEEAAQVLPAGKVRVAVLPCKDANE CHLNGHDREIMEQVWNAGPWIPDGVVSALSLRERIREHLSSEESVGLLFSGCTGINDKTL GARGGEVIMVTSGSGMGKSTFVRQQALQWGTAMGKKVGLAMLQESVEETAEDLIGLHNRV RLRQSDSLKREIIENGKFDQWFDELFGNDTFHLYDSFAEAETDRLLAKLAYMRSGLGCDV IILDHISIVVSASGESDERKMIDNLMTKLKGFAKSTGVVLVVICHLKNPDKGKAHEEGRP VSITDLRGSGALRQLSDTIIALERNQQGDMPNLVLVRILKCRFTGDTGIAGYMEYNKETG WLEPSSYSGEEESHSESTDWSNDTDF gene product 5 DNA polymerase Enterobacteria phage T7 LEVO MIVSAIAANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEVARGGLIVFHN GHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHSNLKDTDMGLLRSGKLPGKRF GSHALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVDGMEWWNFNEEMMDYNVQDVVVTKA LLEKLLSDKHYFPPEIDFTDVGYTTFWSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIE ELYVELAARRSELLRKLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKK PKNKAQREGREPCELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKG APVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSV NPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGITGKPWVQAGIDASGLE LRCLAHFMARFDNGEYAHEILNGDIHTKNQIAAELPTRDNAKTFIYGFLYGAGDEKIGQI VGAGKERGKELKKKFLENTPAIAALRESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRK VHVRSPHAALNTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGC RTEEIAQVVIETAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH Thioredoxin 1 Escherichia coli LEVO MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLN IDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA Primer Enterobacteria phage T7 (DG)(DG)(DT)(DA)(DC)(DA)(DA)(DC)(DT)(DT)(DG)(DA)(DC)(DG)(DA)(DC)(DA)(DT)(DA)(DG)(DC)(DG)(DT)(DG)(DOA) DNA Template Enterobacteria phage T7 (DT)(DT)(DT)(DG)(DG)(DT)(DC)(DA)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DA)(DC)(DG)(DG)(DA)(DG)(DT)(DC)(DG)(DT)(DT)(DT)(DC)(DG)(DA)(DC)(DT)(DC)(DC)(DG)(DT)(DT)(DA)(DT)(DC)(DA)(DC)(DG)(DC)(DT)(DA)(DT)(DG)(DT)(DC)(DG)(DT)(DC)(DA)(DA)(DG)(DT)(DT)(DG)(DT)(DA)(DC)(DC) DNA singleParticle particle 7.5 50.0 Tris 150.0 KCl potassium chloride 3.0 DTT

unspecified

ETHANE 100 295 FEI VITROBOT MARK I

FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) COUNTING 40.0 1 1T8E

1T8E, 1NUI

C1 8.3 FSC 0.143 CUT-OFF RELION 14119 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION 1T8E FLEXIBLE FIT REAL