2019-06-05 REL RCSB RCSB RCSB 2018-12-03 2018-12-12 2019-04-24 2019-06-05 p53 dimer assembly Kelly DF Dearnaley WJ Varano AC Alden NA Varano AC Dearnaley WJ Solares MJ Luqiu WY Liang Y Sheng Z McDonald SM Damiano J McConnell J Dukes MJ Kelly DF Cryo-EM-On-a-Chip: Custom-Designed Substrates for the 3D Analysis of Macromolecules. Small DE 15 e1900918 e1900918 2019 30963664 doi:10.1002/smll.201900918 1613-6829 EMD-0378 associated EM volume p53 dimer assembly p53 dimer assembly 0 1 Homo sapiens Brain Glioblastoma 0.116 p53 Human Brain Glioblastoma LEVO MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAK SVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE RCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPG GSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD singleParticle particle 0.02 7.2 HEPES buffer
20 mM HEPES, pH 7.2, 150 mM NaCl, 10 mM CaCl2, 10 mM MgCl2, 60 mM Imidazole
 SILICON NITRIDE ETHANE 90 298 FEI VITROBOT MARK III
Blot for 6-10 seconds before plunging, dwell time was 1 second, grid platform was SiN chip..
FEI TECNAI SPIRIT FLOOD BEAM BRIGHT FIELD LAB6 120 2.0 -1.5 68000. SIDE ENTRY, EUCENTRIC NITROGEN FEI EAGLE (2k x 2k) 30.0 1 100 5.0 1 12144 RELION 2.1 2AC0
Resolution filtered to ~50 Angstrom
 C2 10.0 FSC 0.5 CUT-OFF RELION 2.1 12144 OTHER RELION 2.1
Empirical Bayesian approach
 OTHER RELION 2.1
Empirical Bayesian approach
 1 12144.0 RELION 2.1 emd_0378.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 0 0 0 100 100 100 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -0.55717945 1.3238852 0.0038478214 0.03233218 4.2 4.2 4.2 0.569 AUTHOR p53 dimer assembly 2AC0 RIGID BODY FIT REAL