2024-10-30 REL RCSB RCSB RCSB 2018-11-29 2019-02-13 2019-03-13 2024-10-30 Cancer Prevention and Research Institute of Texas (CPRIT) RR160088 United States Robert A. Welch Foundation F-1938 United States Army Research Office W911NF-15-0120 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM53655 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM127127 United States Zhou Y Musalgaonkar S ribosome biogenesis, Nmd3, peptidyl transferase center, RIBOSOME Zhou Y Musalgaonkar S Johnson AW Taylor DW Nat Commun UK 10 958 958 2019 30814529 doi:10.1038/s41467-019-08880-0 2041-1723 EMD-0369 other EM volume EMD-0370 other EM volume EMD-0371 other EM volume EMD-0372 other EM volume EMD-0374 other EM volume 6n8n FULLOVERLAP Lsg1-engaged (LE) pre-60S ribosomal subunit Lsg1-engaged (LE) pre-60S ribosomal subunit 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA Saccharomyces cerevisiae S288C ATCC 204508 / S288c 1.0974938749999998 1 GUUUGACCUCAAAUCAGGUAGGAGUACCCGCUGAACUUAAGCAUAUCAAUAAGCGGAGGAAAAGAAACCAACCGGGAUUG CCUUAGUAACGGCGAGUGAAGCGGCAAAAGCUCAAAUUUGAAAUCUGGUACCUUCGGUGCCCGAGUUGUAAUUUGGAGAG GGCAACUUUGGGGCCGUUCCUUGUCUAUGUUCCUUGGAACAGGACGUCAUAGAGGGUGAGAAUCCCGUGUGGCGAGGAGU GCGGUUCUUUGUAAAGUGCCUUCGAAGAGUCGAGUUGUUUGGGAAUGCAGCUCUAAGUGGGUGGUAAAUUCCAUCUAAAG CUAAAUAUUGGCGAGAGACCGAUAGCGAACAAGUACAGUGAUGGAAAGAUGAAAAGAACUUUGAAAAGAGAGUGAAAAAG UACGUGAAAUUGUUGAAAGGGAAGGGCAUUUGAUCAGACAUGGUGUUUUGUGCCCUCUGCUCCUUGUGGGUAGGGGAAUC UCGCAUUUCACUGGGCCAGCAUCAGUUUUGGUGGCAGGAUAAAUCCAUAGGAAUGUAGCUUGCCUCGGUAAGUAUUAUAG CCUGUGGGAAUACUGCCAGCUGGGACUGAGGACUGCGACGUAAGUCAAGGAUGCUGGCAUAAUGGUUAUAUGCCGCCCGU CUUGAAACACGGACCAAGGAGUCUAACGUCUAUGCGAGUGUUUGGGUGUAAAACCCAUACGCGUAAUGAAAGUGAACGUA GGUUGGGGCCUCGCAAGAGGUGCACAAUCGACCGAUCCUGAUGUCUUCGGAUGGAUUUGAGUAAGAGCAUAGCUGUUGGG ACCCGAAAGAUGGUGAACUAUGCCUGAAUAGGGUGAAGCCAGAGGAAACUCUGGUGGAGGCUCGUAGCGGUUCUGACGUG CAAAUCGAUCGUCGAAUUUGGGUAUAGGGGCGAAAGACUAAUCGAACCAUCUAGUAGCUGGUUCCUGCCGAAGUUUCCCU CAGGAUAGCAGAAGCUCGUAUCAGUUUUAUGAGGUAAAGCGAAUGAUUAGAGGUUCCGGGGUCGAAAUGACCUUGACCUA UUCUCAAACUUUAAAUAUGUAAGAAGUCCUUGUUACUUAAUUGAACGUGGACAUUUGAAUGAAGAGCUUUUAGUGGGCCA UUUUUGGUAAGCAGAACUGGCGAUGCGGGAUGAACCGAACGUAGAGUUAAGGUGCCGGAAUACACGCUCAUCAGACACCA CAAAAGGUGUUAGUUCAUCUAGACAGCCGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGAGUGUGUAACAACUCACC GGCCGAAUGAACUAGCCCUGAAAAUGGAUGGCGCUCAAGCGUGUUACCUAUACUCUACCGUCAGGGUUGAUAUGAUGCCC UGACGAGUAGGCAGGCGUGGAGGUCAGUGACGAAGCCUAGACCGUAAGGUCGGGUCGAACGGCCUCUAGUGCAGAUCUUG GUGGUAGUAGCAAAUAUUCAAAUGAGAACUUUGAAGACUGAAGUGGGGAAAGGUUCCACGUCAACAGCAGUUGGACGUGG GUUAGUCGAUCCUAAGAGAUGGGGAAGCUCCGUUUCAAAGGCCUGAUUUUAUGCAGGCCACCAUCGAAAGGGAAUCCGGU UAAGAUUCCGGAACCUGGAUAUGGAUUCUUCACGGUAACGUAACUGAAUGUGGAGACGUCGGCGCGAGCCCUGGGAGGAG UUAUCUUUUCUUCUUAACAGCUUAUCACCCCGGAAUUGGUUUAUCCGGAGAUGGGGUCUUAUGGCUGGAAGAGGCCAGCA CCUUUGCUGGCUCCGGUGCGCUUGUGACGGCCCGUGAAAAUCCACAGGAAGGAAUAGUUUUCAUGCCAGGUCGUACUGAU AACCGCAGCAGGUCUCCAAGGUGAACAGCCUCUAGUUGAUAGAAUAAUGUAGAUAAGGGAAGUCGGCAAAAUAGAUCCGU AACUUCGGGAUAAGGAUUGGCUCUAAGGGUCGGGUAGUGAGGGCCUUGGUCAGACGCAGCGGGCGUGCUUGUGGACUGCU UGGUGGGGCUUGCUCUGCUAGGCGGACUACUUGCGUGCCUUGUUGUAGACGGCCUUGGUAGGUCUCUUGUAGACCGUCGC UUGCUACAAUUAACGAUCAACUUAGAACUGGUACGGACAAGGGGAAUCUGACUGUCUAAUUAAAACAUAGCAUUGCGAUG GUCAGAAAGUGAUGUUGACGCAAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAAAGUGAAGAAAUUCAACCAAGCGCGGG UAAACGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAUUA ACGAGAUUCCCACUGUCCCUAUCUACUAUCUAGCGAAACCACAGCCAAGGGAACGGGCUUGGCAGAAUCAGCGGGGAAAG AAGACCCUGUUGAGCUUGACUCUAGUUUGACAUUGUGAAGAGACAUAGAGGGUGUAGAAUAAGUGGGAGCUUCGGCGCCA GUGAAAUACCACUACCUUUAUAGUUUCUUUACUUAUUCAAUGAAGCGGAGCUGGAAUUCAUUUUCCACGUUCUAGCAUUC AAGGUCCCAUUCGGGGCUGAUCCGGGUUGAAGACAUUGUCAGGUGGGGAGUUUGGCUGGGGCGGCACAUCUGUUAAACGA UAACGCAGAUGUCCUAAGGGGGGCUCAUGGAGAACAGAAAUCUCCAGUAGAACAAAAGGGUAAAAGCCCCCUUGAUUUUG AUUUUCAGUGUGAAUACAAACCAUGAAAGUGUGGCCUAUCGAUCCUUUAGUCCCUCGGAAUUUGAGGCUAGAGGUGCCAG AAAAGUUACCACAGGGAUAACUGGCUUGUGGCAGUCAAGCGUUCAUAGCGACAUUGCUUUUUGAUUCUUCGAUGUCGGCU CUUCCUAUCAUACCGAAGCAGAAUUCGGUAAGCGUUGGAUUGUUCACCCACUAAUAGGGAACGUGAGCUGGGUUUAGACC GUCGUGAGACAGGUUAGUUUUACCCUACUGAUGAAUGUUACCGCAAUAGUAAUUGAACUUAGUACGAGAGGAACAGUUCA UUCGGAUAAUUGGUUUUUGCGGCUGUCUGAUCAGGCAUUGCCGCGAAGCUACCAUCCGCUGGAUUAUGGCUGAACGCCUC UAAGUCAGAAUCCAUGCUAGAACGCGGUGAUUUCUUUGCUCCACACAAUAUAGAUGGAUACGAAUAAGGCGUCCUUGUGG CGUCGCUGAACCAUAGCAGGCUAGCAACGGUGCACUUGGCGGAAAGGCCUUGGGUGCUUGCUGGCGAAUUGCAAUGUCAU UUUGCGUGGGGAUAAAUCAUUUGUAUACGACUUAGAUGUACAACGGGGUAUUGUAAGCAGUAGAGUAGCCUUGUUGUUAC GAUCUGCUGAGAUUAAGCCUUUGUUGUCUGAUUUGU NR_132207.1 5S rRNA Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.038951105 1 GGUUGCGGCCAUAUCUACCAGAAAGCACCGUUUCCCGUCCGAUCAACUGUAGUUAAGCUGGUAAGAGCCUGACCGAGUAG UGUAGUGGGUGACCAUACGCGAAACUCAGGUGCUGCAAUCU CP022977.1 5.8S rRNA Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.050682922 1 AAACUUUCAACAACGGAUCUCUUGGUUCUCGCAUCGAUGAAGAACGCAGCGAAAUGCGAUACGUAAUGUGAAUUGCAGAA UUCCGUGAAUCAUCGAAUCUUUGAACGCACAUUGCGCCCCUUGGUAUUCCAGGGGGCAUGCCUGUUUGAGCGUCAUUU MK044066.1 Tyrosine-protein phosphatase YVH1 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.041242121 1 LEVO MAGNANSVDEEVTRILGGIYLGGIRPIIDHRPLGAEFNITHILSVIKFQVIPEYLIRKGYTLKNIPIDDDDVTDVLQYFD ETNRFIDQCLFPNEVEYSPRLVDFKKKPQRGAVFAHCQAGLSRSVTFIVAYLMYRYGLSLSMAMHAVKRKKPSVEPNENF MEQLHLFEKMGGDFVDFDNPAYKQWKLKQSIKLDPSGSELVSNSGMFKDSESSQDLDKLTEAEKSKVTAVRCKKCRTKLA LSTSFIAHDPPSKESSEGHFIKRAANSHRIIDIQESQANCSHFFIEPLKWMQPELQGKQELEGKFSCPGCSSKVGGYNWK GSRCSCGKWVIPAIHLQTSKVDQFPLQSTALPNMVNFESEKVNR Q02256 3.1.3.48 Eukaryotic translation initiation factor 6 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.026476604999999997 1 LEVO MATRTQFENSNEIGVFSKLTNTYCLVAVGGSENFYSAFEAELGDAIPIVHTTIAGTRIIGRMTAGNRRGLLVPTQTTDQE LQHLRNSLPDSVKIQRVEERLSALGNVICCNDYVALVHPDIDRETEELISDVLGVEVFRQTISGNILVGSYCSLSNQGGL VHPQTSVQDQEELSSLLQVPLVAGTVNRGSSVVGAGMVVNDYLAVTGLDTTAPELSVIESIFRLQDAQPESISGNLRDTL IETYS Q12522 Large subunit GTPase 1 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.072838445 1 LEVO MPPKEAPKKWKAPKGPKPTHRKNKNKLELGRAIKYARQKENAIEYLPDGEMRFTTDKHEANWVKLRSVTQESALDEFLST AALADKDFTADRHSNVKIIRMDSGNDSATSQGFSMTNEQRGNLNAKQRALAKDLIVPRRPEWNEGMSKFQLDRQEKEAFL EWRRKLAHLQESNEDLLLTPFERNIEVWKQLWRVVERSDLVVQIVDARNPLLFRSVDLERYVKESDDRKANLLLVNKADL LTKKQRIAWAKYFISKNISFTFYSALRANQLLEKQKEMGEDYREQDFEEADKEGFDADEKVMEKVKILSIDQLEELFLSK APNEPLLPPLPGQPPLINIGLVGYPNVGKSSTINSLVGAKKVSVSSTPGKTKHFQTIKLSDSVMLCDCPGLVFPNFAYNK GELVCNGVLPIDQLRDYIGPAGLVAERIPKYYIEAIYGIHIQTKSRDEGGNGDIPTAQELLVAYARARGYMTQGYGSADE PRASRYILKDYVNGKLLYVNPPPHLEDDTPYTREECEEFNKDLYVFDRLPDTRKEQVQNAAKAKGIDIVDLARDLNQLTF SAHTGGDTQKEAKSVTHGGKQAALYNAAEDLDRDFFKMNNVEGRLSTPFHKVQNSSAGKRHNKKNKSKNAKSKVFSIENN P53145 3.6.1.- 60S ribosomal export protein NMD3 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.05916704699999999 1 LEVO MEFTPIDPHQHQNAATLLCCNCGTPIDGSTGLVMCYDCIKLTVDITQGIPREANISFCRNCERFLQPPGQWIRAELESRE LLAICLRRLKGLTKVRLVDASFIWTEPHSRRIRIKLTVQGEAMTNTIIQQTFEVEYIVIAMQCPDCARSYTTNTWRATVQ IRQKVPHKRTFLFLEQLILKHNAHVDTISISEAKDGLDFFYAQKNHAVKMIDFLNAVVPIKHKKSEELISQDTHTGASTY KFSYSVEIVPICKDDLVVLPKKLAKSMGNISQFVLCSKISNTVQFMDPTTLQTADLSPSVYWRAPFNALADVTQLVEFIV LDVDSTGISRGNRVLADITVARTSDLGVNDQVYYVRSHLGGICHAGDSVMGYFIANSNYNSDLFDGLNIDYVPDVVLVKK LYQRKSKKSRHWKLKRMAKEHKDIDASLDYNSRAQKQEMERAEKDYELFLQELEEDAELRQSVNLYKNREANVPPEEHEM DEDEDEDAPQINIDELLDELDEMTLEDGVENTPVESQQ P38861 60S ribosomal protein L2-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.027463574 1 LEVO MGRVIRNQRKGAGSIFTSHTRLRQGAAKLRTLDYAERHGYIRGIVKQIVHDSGRGAPLAKVVFRDPYKYRLREEIFIANE GVHTGQFIYAGKKASLNVGNVLPLGSVPEGTIVSNVEEKPGDRGALARASGNYVIIIGHNPDENKTRVRLPSGAKKVISS DARGVIGVIAGGGRVDKPLLKAGRAFHKYRLKRNSWPKTRGVAMNPVDHPHGGGNHQHIGKASTISRGAVSGQKAGLIAA RRTGLLRGSQKTQD P0CX45 60S ribosomal protein L3 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.043850793 1 LEVO MSHRKYEAPRHGHLGFLPRKRAASIRARVKAFPKDDRSKPVALTSFLGYKAGMTTIVRDLDRPGSKFHKREVVEAVTVVD TPPVVVVGVVGYVETPRGLRSLTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQDGAGIERELARIKKYASVVRV LVHTQIRKTPLAQKKAHLAEIQLNGGSISEKVDWAREHFEKTVAVDSVFEQNEMIDAIAVTKGHGFEGVTHRWGTKKLPR KTHRGLRKVACIGAWHPAHVMWSVARAGQRGYHSRTSINHKIYRVGKGDDEANGATSFDRTKKTITPMGGFVHYGEIKND FIMVKGCIPGNRKRIVTLRKSLYTNTSRKALEEVSLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL P14126 60S ribosomal protein L4-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.039159124999999996 1 LEVO MSRPQVTVHSLTGEATANALPLPAVFSAPIRPDIVHTVFTSVNKNKRQAYAVSEKAGHQTSAESWGTGRAVARIPRVGGG GTGRSGQGAFGNMCRGGRMFAPTKTWRKWNVKVNHNEKRYATASAIAATAVASLVLARGHRVEKIPEIPLVVSTDLESIQ KTKEAVAALKAVGAHSDLLKVLKSKKLRAGKGKYRNRRWTQRRGPLVVYAEDNGIVKALRNVPGVETANVASLNLLQLAP GAHLGRFVIWTEAAFTKLDQVWGSETVASSKVGYTLPSHIISTSDVTRIINSSEIQSAIRPAGQATQKRTHVLKKNPLKN KQVLLRLNPYAKVFAAEKLGSKKAEKTGTKPAAVFTETLKHD P10664 60S ribosomal protein L5 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.033764828 1 LEVO MAFQKDAKSSAYSSRFQTPFRRRREGKTDYYQRKRLVTQHKAKYNTPKYRLVVRFTNKDIICQIISSTITGDVVLAAAYS HELPRYGITHGLTNWAAAYATGLLIARRTLQKLGLDETYKGVEEVEGEYELTEAVEDGPRPFKVFLDIGLQRTTTGARVF GALKGASDGGLYVPHSENRFPGWDFETEEIDPELLRSYIFGGHVSQYMEELADDDEERFSELFKGYLADDIDADSLEDIY TSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALAGQQ P26321 60S ribosomal protein L6-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.020000564 1 LEVO MSAQKAPKWYPSEDVAALKKTRKAARPQKLRASLVPGTVLILLAGRFRGKRVVYLKHLEDNTLLISGPFKVNGVPLRRVN ARYVIATSTKVSVEGVNVEKFNVEYFAKEKLTKKEKKEANLFPEQQNKEIKAERVEDQKVVDKALIAEIKKTPLLKQYLS ASFSLKNGDKPHMLKF Q02326 60S ribosomal protein L7-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.027686280999999997 1 LEVO MAAEKILTPESQLKKSKAQQKTAEQVAAERAARKAANKEKRAIILERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQ HKLVFVVRIKGINKIPPKPRKVLQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQR VPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPRKFKHFIQGGSFGNREEFINKLV KSMN P05737 60S ribosomal protein L8-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.028175819999999997 1 LEVO MAPGKKVAPAPFGAKSTKSNKTRNPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPPTIAQFQY TLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQDASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPI ELVVFLPALCKKMGVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFADKYDEVKKHWGGGILGN KAQAKMDKRAKNSDSA P17076 60S ribosomal protein L9-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.021605061000000002 1 LEVO MKYIQTEQQIEVPEGVTVSIKSRIVKVVGPRGTLTKNLKHIDVTFTKVNNQLIKVAVHNGGRKHVAALRTVKSLVDNMIT GVTKGYKYKMRYVYAHFPINVNIVEKDGAKFIEVRNFLGDKKIRNVPVRDGVTIEFSTNVKDEIVLSGNSVEDVSQNAAD LQQICRVRNKDIRKFLDGIYVSHKGFITEDL P05738 60S ribosomal protein L11-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.019755691 1 LEVO MSAKAQNPMRDLKIEKLVLNISVGESGDRLTRASKVLEQLSGQTPVQSKARYTVRTFGIRRNEKIAVHVTVRGPKAEEIL ERGLKVKEYQLRDRNFSATGNFGFGIDEHIDLGIKYDPSIGIFGMDFYVVMNRPGARVTRRKRCKGTVGNSHKTTKEDTV SWFKQKYDADVLDK P0C0W9 60S ribosomal protein L13-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.022604164 1 LEVO MAISKNLPILKNHFRKHWQERVKVHFDQAGKKVSRRNARATRAAKIAPRPLDLLRPVVRAPTVKYNRKVRAGRGFTLAEV KAAGLTAAYARTIGIAVDHRRQNRNQEIFDANVQRLKEYQSKIIVFPRNGKAPEAEQVLSAAATFPIAQPATDVEARAVQ DNGESAFRTLRLARSEKKFRGIREKRAREKAEAEAEKKK Q12690 60S ribosomal protein L14-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.015195066 1 LEVO MSTDSIVKASNWRLVEVGRVVLIKKGQSAGKLAAIVEIIDQKKVLIDGPKAGVPRQAINLGQVVLTPLTFALPRGARTAT VSKKWAAAAVCEKWAASSWAKKIAQRERRAALTDFERFQVMVLRKQKRYTVKKALAKA P36105 60S ribosomal protein L42-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.012246657999999999 1 LEVO MVNVPKTRKTYCKGKTCRKHTQHKVTQYKAGKASLFAQGKRRYDRKQSGFGGQTKPVFHKKAKTTKKVVLRLECVKCKTR AQLTLKRCKHFELGGEKKQKGQALQF P0CX27 60S ribosomal protein L43-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.010112952 1 LEVO MAKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHARYDCSFCGKKTVKRGAAGIWTCSCCKKTVAGGAYTVSTAAAATVR STIRRLREMVEA P0CX25 Ribosomal Protein uL1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ATCC 204508 / S288c 0.018485723000000003 1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) 60S ribosomal protein L15-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.024482357 1 LEVO MGAYKYLEELQRKKQSDVLRFLQRVRVWEYRQKNVIHRAARPTRPDKARRLGYKAKQGFVIYRVRVRRGNRKRPVPKGAT YGKPTNQGVNELKYQRSLRATAEERVGRRAANLRVLNSYWVNQDSTYKYFEVILVDPQHKAIRRDARYNWICDPVHKHRE ARGLTATGKKSRGINKGHKFNNTKAGRRKTWKRQNTLSLWRYRK P05748 60S ribosomal protein L16-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.022247226999999998 1 LEVO MSVEPVVVIDGKGHLVGRLASVVAKQLLNGQKIVVVRAEELNISGEFFRNKLKYHDFLRKATAFNKTRGPFHFRAPSRIF YKALRGMVSHKTARGKAALERLKVFEGIPPPYDKKKRVVVPQALRVLRLKPGRKYTTLGKLSTSVGWKYEDVVAKLEAKR KVSSAEYYAKKRAFTKKVASANATAAESDVAKQLAALGY P26784 60S ribosomal protein L17-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.020589517999999998 1 LEVO MARYGATSTNPAKSASARGSYLRVSFKNTRETAQAINGWELTKAQKYLEQVLDHQRAIPFRRFNSSIGRTAQGKEFGVTK ARWPAKSVKFVQGLLQNAAANAEAKGLDATKLYVSHIQVNQAPKQRRRTYRAHGRINKYESSPSHIELVVTEKEEAVAKA AEKKVVRLTSRQRGRIAAQKRIAA P05740 60S ribosomal protein L18-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.020609251999999998 1 LEVO MGIDHTSKQHKRSGHRTAPKSDNVYLKLLVKLYTFLARRTDAPFNKVVLKALFLSKINRPPVSVSRIARALKQEGAANKT VVVVGTVTDDARIFEFPKTTVAALRFTAGARAKIVKAGGECITLDQLAVRAPKGQNTLILRGPRNSREAVRHFGMGPHKG KAPRILSTGRKFERARGRRRSKGFKV P0CX49 60S ribosomal protein L19-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.021762315999999997 1 LEVO MANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIVKKAVTVHSKSRTRAHAQSKREGRHSGYGK RKGTREARLPSQVVWIRRLRVLRRLLAKYRDAGKIDKHLYHVLYKESKGNAFKHKRALVEHIIQAKADAQREKALNEEAE ARRLKNRAARDRRAQRVAEKRDALLKEDA P0CX82 60S ribosomal protein L20-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.020478852 1 LEVO MAHFKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYFLQKLHKVKKASGEIVSINQINEAHPTKVKNFGVWVR YDSRSGTHNMYKEIRDVSRVAAVETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKFPLPHRVQKST KTFSYKRPSTFY P0CX23 60S ribosomal protein L21-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.018279266 1 LEVO MGKSHGYRSRTRYMFQRDFRKHGAVHLSTYLKVYKVGDIVDIKANGSIQKGMPHKFYQGKTGVVYNVTKSSVGVIINKMV GNRYLEKRLNLRVEHIKHSKCRQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTLAPVPYETFI Q02753 60S ribosomal protein L22-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.013711359 1 LEVO MAPNTSRKQKIAKTFTVDVSSPTENGVFDPASYAKYLIDHIKVEGAVGNLGNAVTVTEDGTVVTVVSTAKFSGKYLKYLT KKYLKKNQLRDWIRFVSTKTNEYRLAFYQVTPEEDEEEDEE P05749 60S ribosomal protein L23-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.01449395 1 LEVO MSGNGAQGTKFRISLGLPVGAIMNCADNSGARNLYIIAVKGSGSRLNRLPAASLGDMVMATVKKGKPELRKKVMPAIVVR QAKSWRRRDGVFLYFEDNAGVIANPKGEMKGSAITGPVGKECADLWPRVASNSGVVV P0CX41 60S ribosomal protein L24-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.017661717 1 LEVO MKVEIDSFSGAKIYPGRGTLFVRGDSKIFRFQNSKSASLFKQRKNPRRIAWTVLFRKHHKKGITEEVAKKRSRKTVKAQR PITGASLDLIKERRSLKPEVRKANREEKLKANKEKKKAEKAARKAEKAKSAGTQSSKFSKQQAKGAFQKVAATSR P04449 60S ribosomal protein L25 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.015787612 1 LEVO MAPSAKATAAKKAVVKGTNGKKALKVRTSATFRLPKTLKLARAPKYASKAVPHYNRLDSYKVIEQPITSETAMKKVEDGN ILVFQVSMKANKYQIKKAVKELYEVDVLKVNTLVRPNGTKKAYVRLTADYDALDIANRIGYI P04456 60S ribosomal protein L26-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.014265783999999998 1 LEVO MAKQSLDVSSDRRKARKAYFTAPSSQRRVLLSAPLSKELRAQYGIKALPIRRDDEVLVVRGSKKGQEGKISSVYRLKFAV QVDKVTKEKVNGASVPINLHPSKLVITKLHLDKDRKALIQRKGGKLE P05743 60S ribosomal protein L27-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.01556836 1 LEVO MAKFLKAGKVAVVVRGRYAGKKVVIVKPHDEGSKSHPFGHALVAGIERYPLKVTKKHGAKKVAKRTKIKPFIKVVNYNHL LPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF P0C2H6 60S ribosomal protein L28 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.016761666 1 LEVO MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRINMDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWT LIPEDKRDQYLKSASKETAPVIDTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA P02406 60S ribosomal protein L29 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.0066918839999999995 1 LEVO MAKSKNHTAHNQTRKAHRNGIKKPKTYKYPSLKGVDPKFRRNHKHALHGTAKALAAAKK P05747 60S ribosomal protein L30 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.011430363999999998 1 LEVO MAPVKSQESINQKLALVIKSGKYTLGYKSTVKSLRQGKSKLIIIAANTPVLRKSELEYYAMLSKTKVYYFQGGNNELGTA VGKLFRVGVVSILEAGDSDILTTLA P14120 60S ribosomal protein L31-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.012980157999999999 1 LEVO MAGLKDVVTREYTINLHKRLHGVSFKKRAPRAVKEIKKFAKLHMGTDDVRLAPELNQAIWKRGVKGVEYRLRLRISRKRN EEEDAKNPLFSYVEPVLVASAKGLQTVVVEEDA P0C2H8 60S ribosomal protein L32 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.014809441 1 LEVO MASLPHPKIVKKHTKKFKRHHSDRYHRVAENWRKQKGIDSVVRRRFRGNISQPKIGYGSNKKTKFLSPSGHKTFLVANVK DLETLTMHTKTYAAEIAHNISAKNRVVILARAKALGIKVTNPKGRLALEA P38061 60S ribosomal protein L33-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.012177129999999998 1 LEVO MAESHRLYVKGKHLSYQRSKRVNNPNVSLIKIEGVATPQDAQFYLGKRIAYVYRASKEVRGSKIRVMWGKVTRTHGNSGV VRATFRNNLPAKTFGASVRIFLYPSNI P05744 60S ribosomal protein L34-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.013673195999999999 1 LEVO MAQRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSR CANCVKERIIRAFLIEEQKIVKKVVKEQTEAAKKSEKKAKK P87262 60S ribosomal protein L35-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.01394264 1 LEVO MAGVKAYELRTKSKEQLASQLVDLKKELAELKVQKLSRPSLPKIKTVRKSIACVLTVINEQQREAVRQLYKGKKYQPKDL RAKKTRALRRALTKFEASQVTEKQRKKQIAFPQRKYAIKA P0CX84 60S ribosomal protein L36-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.011151259 1 LEVO MTVKTGIAIGLNKGKKVTSMTPAPKISYKKGAASNRTKFVRSLVREIAGLSPYERRLIDLIRNSGEKRARKVAKKRLGSF TRAKAKVEEMNNIIAASRRH P05745 60S ribosomal protein L37-A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.009877395 1 LEVO MGKGTPSFGKRHNKSHTLCNRCGRRSFHVQKKTCSSCGYPAAKTRSYNWGAKAKRRHTTGTGRMRYLKHVSRRFKNGFQT GSASKASA P49166 60S ribosomal protein L38 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.008845561 1 LEVO MAREITDIKQFLELTRRADVKTATVKINKKLNKAGKPFRQTKFKVRGSSSLYTLVINDAGKAKKLIQSLPPTLKVNRL P49167 60S ribosomal protein L39 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.0063586400000000005 1 LEVO MAAQKSFRIKQKMAKAKKQNRPLPQWIRLRTNNTIRYNAKRRNWRRTKMNI P04650 Ubiquitin-60S ribosomal protein L40 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.014583076999999998 1 LEVO MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEP SLKALASKYNCDKSVCRKCYARLPPRATNCRKRKCGHTNQLRPKKKLK P0CH08 Ribosomal protein L12 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ATCC 204508 / S288c 0.015951983 1 LEVO MPPKFDPNE(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)ATKEFKGIKVTVQLKIQNRQAAASVVPSASSLVIT ALKEPPRDRKKDKNVKHSGNIQLDEII(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)EN singleParticle particle 7.5 CARBON HOLEY

unspecified

ETHANE 100 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 1.0 2.5 22500.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 6.0 40.0 1 5T62 C1 3.8 FSC 0.143 CUT-OFF RELION 54188 OTHER OTHER 5T62 PDB experimental model RIGID BODY FIT REAL