2019-03-20 REL RCSB RCSB RCSB 2018-11-23 2018-12-19 2019-03-20 2019-03-20 Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus Marcandalli J Fiala B Ols S Perotti M van der Schueren W Snijder S Hodge E Benhaim M Ravichandran R Carter L Sheffler W Brunner L Lawrenz M Dubois P Lanzavecchia A Sallusto F Lee KK Veesler D Correnti CE Stewart LJ Baker D Lore K Perez L King NP Marcandalli J Fiala B Ols S Perotti M de van der Schueren W Snijder J Hodge E Benhaim M Ravichandran R Carter L Sheffler W Brunner L Lawrenz M Dubois P Lanzavecchia A Sallusto F Lee KK Veesler D Correnti CE Stewart LJ Baker D Lore K Perez L King NP Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus. Cell 176 1420 1431.e17 2019 30849373 doi:10.1016/j.cell.2019.01.046 1097-4172 EMD-0350 associated EM volume DS-Cav1-I53-50 nanoparticle DS-Cav1-I53-50 nanoparticle 0 1 2 Respiratory syncytial virus Homo sapiens Engineered respiratory syncytial virus F protein (DS-Cav-1) fused to the foldon and to the trimeric component of I53-50 (DS-Cav1-I53-50A) Respiratory syncytial virus LEVO MELLILKANAITTILTAVTFCFASGQNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDAKVKLIKQELDKYKNAVTELQLLMQSTPATNNRARRELPRFMNYTLNNAKKTNVTLSKKRKRRFLGFLLGVGSAIASGVAVCKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTFKVLDLKNYIDKQLLPILNKQSCSISNIETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMCIIKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPSEVNLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRGYIPEAPRDGQAYVRKDGEWVLLSTFLGSGSHHHHHHHHGGSGGSGSEKAAKAEEAARKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIEITFTVPDADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAVESGAEFIVSPHLDEEISQFCKEKGVFYMPGVMTPTELVKAMKLGHTILKLFPGEVVGPQFVKAMKGPFPNVKFVPTGGVNLDNVCEWFKAGVLAVGVGSALVKGTPDEVREKAKAFVEKIRGCTE Pentameric component of I53-50 (I53-50B) Respiratory syncytial virus LEVO MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDIGGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEFVASAVINGMMNVQLNTGVPVLSAVLTPHNYDKSKAHTLLFLALFAVKGMEAARACVEILAAREKIAAGSLEHHHHHH singleParticle particle 8.0
unspecified
 ETHANE
FEI TECNAI 20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 GATAN K2 SUMMIT (4k x 4k) 45.0 1 Computationally designed model I 6.3 FSC 0.143 CUT-OFF 1200 PROJECTION MATCHING PROJECTION MATCHING emd_0350.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 600.0 600.0 600.0 90.0 90.0 90.0 X Y Z -0.038507614 0.17572236 0.0013413099 0.01018412 2.5 2.5 2.5 0.06 AUTHOR DS-Cav1-I53-50 nanoparticle