2024-11-06 REL RCSB RCSB RCSB 2018-11-20 2019-02-06 2019-02-06 2024-11-06 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) R35 GM122588 United States MicroED structure of Proteinase K at 2.75A resolution from a single milled crystal. Martynowycz MW Zhao W broad-spectrum serum proteinase, HYDROLASE Martynowycz MW Zhao W Hattne J Jensen GJ Gonen T Collection of Continuous Rotation MicroED Data from Ion Beam-Milled Crystals of Any Size. Structure UK 27 545 548.e2 2019 30661853 doi:10.1016/j.str.2018.12.003 1878-4186 2005 STRUE6 6n4u FULLOVERLAP Proteinase K Proteinase K 0 1 Engyodontium album 0.02893 Proteinase K Engyodontium album 0.028958791 1 Engyodontium album LEVO AAQTNAPWGLARISSTSPGTSTYYYDESAGQGSCVYVIDTGIEASHPEFEGRAQMVKTYYYSSRDGNGHGTHCAGTVGSR TYGVAKKTQLFGVKVLDDNGSGQYSTIIAGMDFVASDKNNRNCPKGVVASLSLGGGYSSSVNSAAARLQSSGVMVAVAAG NNNADARNYSPASEPSVCTVGASDRYDRRSSFSNYGSVLDIFGPGTDILSTWIGGSTRSISGTSMATPHVAGLAAYLMTL GKTTAASACRYIADTANKGDLSNIPFGTVNLLAYNNYQA P06873 3.4.21.64 CALCIUM ION 4.0078e-05 2 CA SULFATE ION 9.606299999999999e-05 1 SO4 water 1.8015e-05 23 HOH electronCrystallography threeDArray 20 7.5 Quantifoil R2/2 COPPER 200 CARBON HOLEY 12.0 GLOW DISCHARGE OTHER ETHANE 100 273.0 FEI VITROBOT MARK IV
Proteinase K purchased from Sigma.
 FEI TALOS ARCTICA FLOOD BEAM DIFFRACTION FIELD EMISSION GUN 200 100.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 77.0 90.0 FEI CETA (4k x 4k) 4096 4096 1 100 2.0 0.04
Continuous rotation from -30 to +30 with a rotation rate of 0.2 degrees per second and a readout every 3 seconds.
 1700 1
This was the new CetaD.
2.75 DIFFRACTION PATTERN/LAYERLINES PHENIX 2.8.2 31013 10807 88.400000000000006 0.39 0.47 22.41 22.41 0 2.75 2.75 3.1483 1831 28.109999999999999 88.230000000000004 2.9 emd_0343.map.gz 96 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 75 79 72 -38 -63 -16 100 100 160 67.46 67.46 106.67 90.0 90.0 90.0 Z Y X -3.9357493 5.0805335 0.00043542887 0.97957665 0.6746 0.6746 0.6666875 1.46937 AUTHOR MicroED 2Fo-Fc map of proteinase K at 2.75A resolution from a single milled crystal. 5I9S PDB experimental model RIGID BODY FIT R RECIPROCAL 21.100000000000001