2023-10-25 REL RCSB RCSB RCSB 2018-11-16 2018-12-12 2018-12-12 2023-10-25 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) R01 GM066087 United States National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) R01 GM128507 United States Howard Hughes Medical Institute (HHMI) United States MicroED Structure of the CTD-SP1 fragment of HIV-1 Gag with bound maturation inhibitor Bevirimat. Purdy MD Shi D Bevirimat, HIV-1 Gag, MicroED, Immature Hexagonal Lattice, VIRAL PROTEIN Purdy MD Shi D Chrustowicz J Hattne J Gonen T Yeager M MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat. Proc. Natl. Acad. Sci. U.S.A. US 115 13258 13263 2018 30530702 doi:10.1073/pnas.1806806115 1091-6490 0040 PNASA6 6n3u FULLOVERLAP CTD-SP1 fragment of HIV-1 Gag with bound maturation inhibitor Bevirimat CTD-SP1 fragment of HIV-1 Gag with bound maturation inhibitor Bevirimat 0 1 Human immunodeficiency virus 1 NL4-3 .01217387 CTD-SP1 fragment of HIV-1 Gag Human immunodeficiency virus 1 0.012192895 6 Escherichia coli LEVO HMHHHHHHGGSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGATLEEMM TACQGVGGPGHKARVLAEAMSQVTNTATIM A0A248SMC7 electronCrystallography threeDArray 0.4 7.0 0.1 Bis-Tris Propane 1.1 LiSO4 Lithium sulfate Quantifoil R2/4 COPPER 300 CARBON HOLEY ARRAY GLOW DISCHARGE
30 seconds each side
 ETHANE FEI VITROBOT MARK IV
10 microliters of 0.8 mg/ml protein was mixed with 10 microliters of a solution of 0.64 mM Bevirimat in DMSO/water.
 FEI TECNAI F20 FLOOD BEAM DIFFRACTION FIELD EMISSION GUN 200 GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER NITROGEN TVIPS TEMCAM-F416 (4k x 4k) 5 5.0 0.05
Data from 5 crystals was merged for structure determination.
 2000 1 DIFFRACTION PATTERN/LAYERLINES PHENIX dev-2747 AIMLESS 98015 13021 83.400000000000006 0.544 0.509 0 1.0 0 2.9 2.9 19.9 11635 1.0 83.400000000000006 7.5 2.9 3.0 1137 1.0 82.299999999999997 7.5 emd_0337.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 389 514 528 -98 -266 -153 528 514 389 100.4784 97.8142 74.0267 90.0 95.042 90.0 Z Y X -4.809102 7.1038866 -0.0016254015 0.9474982 0.1903 0.1903 0.1903 1.3 AUTHOR MicroED map of the CTD-SP1 fragment of HIV-1 Gag with bound maturation inhibitor Bevirimat. 5I4T PDB experimental model FLEXIBLE FIT RECIPROCAL 52.600000000000001