2023-10-11 REL RCSB RCSB RCSB 2018-11-15 2018-12-12 2018-12-12 2023-10-11 National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) 5 P50 GM082545-10 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01 GM066087 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) P50 GM082545 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01 GM12850 United States MicroED Structure of the CTD-SP1 fragment of HIV-1 Gag Purdy MD Shi D Bevirimat, HIV-1 Gag, MicroED, Immature Hexagonal Lattice, VIRAL PROTEIN Purdy MD Shi D Chrustowicz J Hattne J Gonen T Yeager M MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat. Proc. Natl. Acad. Sci. U.S.A. US 115 13258 13263 2018 30530702 doi:10.1073/pnas.1806806115 1091-6490 0040 PNASA6 6n3j FULLOVERLAP CTD-SP1 fragment of HIV-1 Gag CTD-SP1 fragment of HIV-1 Gag 0 1 Human immunodeficiency virus 1 NL4-3 .01217387 CTD-SP1 fragment of HIV-1 Gag Human immunodeficiency virus 1 0.012192895 6 Escherichia coli LEVO HMHHHHHHGGSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGATLEEMM TACQGVGGPGHKARVLAEAMSQVTNTATIM A0A248SMC7 electronCrystallography threeDArray 0.8 7.0 0.1 Bis-Tris Propane 1.1 LiSO4 Lithium sulfate Quantifoil R2/4 COPPER 300 CARBON HOLEY ARRAY GLOW DISCHARGE AIR
Glow discharge 30s each side
 ETHANE FEI VITROBOT MARK IV FEI TECNAI F20 FLOOD BEAM DIFFRACTION FIELD EMISSION GUN 200 TVIPS TEMCAM-F416 (4k x 4k) 6 8.0 0.05
Data from 6 crystals was merged for structure determination.
 2000 1 3.0 DIFFRACTION PATTERN/LAYERLINES PHENIX dev-2747 AIMLESS 10480 10480 79.799999999999997 0.255 0.564 0 1.0 0 3.0 3.0 19.9 10480 1.0 79.799999999999997 5.7 3.0 3.2 1006 1.0 78.700000000000003 5.7 emd_0335.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 151 217 218 -63 112 -125 218 217 151 99.65216 97.49376 71.66913 90.0 90.0 90.0 Z Y X -4.150162 3.6646461 0.0021039585 0.6012653 0.45712 0.44928 0.47463 1.1 AUTHOR Electron crystallographic map from 3D crystals (MicroED) of a CTD-SP1 fragment of HIV-1 Gag. 5I4T PDB experimental model FLEXIBLE FIT RECIPROCAL 41.100000000000001