Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition

2019-07-17 REL PDBe PDBe PDBe 2018-10-29 2019-07-17 2019-07-17 2019-07-17 Spanish Ministry of Economy and Competitiveness BFU2016-75984 Spain Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition Valpuesta JM Arranz R Martin-Benito J Franco A Arranz R Fernandez-Rivero N Velazquez-Campoy A Martin-Benito J Segura J Prado A Valpuesta JM Muga A Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition. Sci Rep UK 9 9487 9487 2019 31263230 doi:10.1038/s41598-019-45726-7 2045-2322 EMD-0323 associated EM volume Nucleoplasmin-histone octamer complex Nucleoplasmin-histone octamer complex 0 1 2 3 4 5 0.32 Nucleoplasmin 1 1 Xenopus laevis Escherichia coli Histones 1 2 3 4 5 Gallus gallus Nucleoplasmin Xenopus laevis Escherichia coli LEVO MASTVSNTSK LEKPVSLIWG CELNEQDKTF EFKVEDDEEK CEHQLALRTV CLGDKAKDEF NIVEIVTQEE GAEKSVPIAT LKPSILPMAT MVGIELTPPV TFRLKAGSGP LYISGQHVAM EEDYSWAEEE DEGEAEGEEE EEEEEDQESP PKAVKRPAAT KKAGQAKKKK LDKEDESSEE DSPTKKGKGA GRGRKPAAKK Histone H2A Gallus gallus LEVO MSGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK Histone H2B Gallus gallus LEVO MPEPAKSAPA PKKGSKKAVT KTQKKGDKKR KKSRKESYSI YVYKVLKQVH PDTGISSKAM GIMNSFVNDI FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK Histone H3 Gallus gallus LEVO MARTKQTARK FTGGKAPRKQ LATKAARKSA PSTGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSAAI GALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA Histone H4 Gallus gallus LEVO MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG singleParticle particle 0.1 7.5

240 mM NaCl, 2 mM MgCl2, 25 mM Hepes pH 7.5

Quantifoil R1.2/1.3 COPPER/RHODIUM 300 GLOW DISCHARGE ETHANE LEICA EM CPC

FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 80500. FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON II (4k x 4k) INTEGRATING 4096 4096 5 897 2.5 1 166976 CTFFIND BACK PROJECTION 14.7 FSC 0.5 CUT-OFF RELION 11438 MAXIMUM LIKELIHOOD Xmipp MAXIMUM LIKELIHOOD RELION emd_0323.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 52 52 52 0 0 0 52 52 52 226.2 226.2 226.2 90.0 90.0 90.0 X Y Z -0.07121008 0.26907974 0.011250387 0.03724214 4.35 4.35 4.35 0.094 AUTHOR ::::EMDATABANK.org::::EMD-0323:::: Complex between two nucleoplasmin molecules (each one a homopentamer) and an histone octamer 1K5J 1AOI RIGID BODY FIT REAL