<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_2_0/emdb.xsd" emdb_id="EMD-0307" version="3.0.2.0">
    <admin>
        <current_status>
            <date>2020-12-02</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2018-10-19</deposition>
            <header_release>2018-11-07</header_release>
            <map_release>2018-11-07</map_release>
            <update>2020-12-02</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Wellcome Trust</funding_body>
                <code>109161/Z/15/A</code>
                <country>United Kingdom</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Royal Society</funding_body>
                <code>DH130048</code>
                <country>United Kingdom</country>
            </grant_reference>
        </grant_support>
        <title>Representative tomogram of the COPII in vitro reconstitution used for subtomogram averaging</title>
        <authors_list>
            <author>Zanetti G</author>
            <author>Hutchings J</author>
            <author>Hagen WJH</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Hutchings J</author>
                    <author order="2">Stancheva V</author>
                    <author order="3">Miller EA</author>
                    <author order="4">Zanetti G</author>
                    <title>Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>9</volume>
                    <first_page>4154</first_page>
                    <last_page>4154</last_page>
                    <year>2018</year>
                    <external_references type="PUBMED">30297805</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-018-06577-4</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-0044</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Subtomogram average of membrane-assembled COPII inner coat</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0307</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>In vitro reconstitution of COPII membrane deformation with giant unilamellar vesicles (GUVs).</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>In vitro reconstitution of COPII membrane deformation with giant unilamellar vesicles (GUVs).</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="7108">Spodoptera frugiperda</recombinant_organism>
                </recombinant_expression>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>helicalArray</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>6.8</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">277</chamber_temperature>
                        <instrument>FEI VITROBOT MARK II</instrument>
                        <details></details>
                    </vitrification>
                    <fiducial_markers_list>
                        <fiducial_marker>
                            <manufacturer>BBI</manufacturer>
                            <diameter units="nanometer">5</diameter>
                        </fiducial_marker>
                    </fiducial_markers_list>
                    <sectioning>
                        <other_sectioning>NO SECTIONING</other_sectioning>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>OTHER</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">0.0015</nominal_defocus_min>
                    <nominal_defocus_max units="µm">0.0035</nominal_defocus_max>
                    <nominal_magnification>105000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">3.6</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <number_images_used>40</number_images_used>
                </final_reconstruction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="5">
        <file>emd_0307.map.gz</file>
        <symmetry>
            <space_group>0</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS SIGNED INTEGER (2 BYTES)</data_type>
        <dimensions>
            <col>3708</col>
            <row>3708</row>
            <sec>1666</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>3708</x>
            <y>3708</y>
            <z>1666</z>
        </spacing>
        <cell>
            <a units="Å">1.0</a>
            <b units="Å">1.0</b>
            <c units="Å">1666.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-32768</minimum>
            <maximum>32767</maximum>
            <average>-584.7793</average>
            <std>-1</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">0.00026968715</x>
            <y units="Å">0.00026968715</y>
            <z units="Å">1.0</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0307::::</label>
        <annotation_details>*** This is a dummy file ***
Representative unbinned 3D-CTF corrected tomogram used for subtomogram averaging of COPII assembled on membranes.</annotation_details>
    </map>
</emd>
