2025-10-01 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin _em_admin.last_update DATABASE_REFERENCES citation _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last OTHER atom_sites _atom_sites.fract_transf_matrix[1][1] _atom_sites.fract_transf_matrix[2][2] _atom_sites.fract_transf_matrix[3][3] REMEDIATION REPOSITORY Carbohydrate remediation STRUCTURE_SUMMARY atom_site chem_comp em_entity_assembly em_imaging_optics entity pdbx_branch_scheme pdbx_chem_comp_identifier pdbx_entity_branch pdbx_entity_branch_descriptor pdbx_entity_branch_link pdbx_entity_branch_list pdbx_entity_nonpoly pdbx_nonpoly_scheme pdbx_struct_assembly_gen struct_asym struct_conn struct_site struct_site_gen _atom_site.B_iso_or_equiv _atom_site.Cartn_x _atom_site.Cartn_y _atom_site.Cartn_z _atom_site.auth_asym_id _atom_site.auth_atom_id _atom_site.auth_comp_id _atom_site.auth_seq_id _atom_site.label_asym_id _atom_site.label_atom_id _atom_site.label_comp_id _atom_site.label_entity_id _atom_site.type_symbol _chem_comp.name _chem_comp.pdbx_synonyms _chem_comp.type _em_entity_assembly.entity_id_list _em_imaging_optics.phase_plate _pdbx_struct_assembly_gen.asym_id_list _struct_conn.pdbx_dist_value _struct_conn.pdbx_leaving_atom_flag _struct_conn.pdbx_role _struct_conn.ptnr1_auth_asym_id _struct_conn.ptnr1_auth_seq_id _struct_conn.ptnr1_label_asym_id _struct_conn.ptnr1_label_atom_id _struct_conn.ptnr2_auth_asym_id _struct_conn.ptnr2_auth_seq_id _struct_conn.ptnr2_label_asym_id DATABASE_REFERENCES database_2 pdbx_audit_support _database_2.pdbx_DOI _database_2.pdbx_database_accession _pdbx_audit_support.funding_organization STRUCTURE_SUMMARY chem_comp_atom chem_comp_bond em_admin pdbx_entry_details pdbx_modification_feature pdbx_validate_close_contact struct_conn _em_admin.last_update PDBe PDBe 2018-10-08 2018-11-07 2019-01-02 2025-10-01 Medical Research Council (United Kingdom) MR/L009609/1 United Kingdom Medical Research Council (United Kingdom) MC_UP_1201/15 United Kingdom Medical Research Council (United Kingdom) MC_UP_A025_1013 United Kingdom Biotechnology and Biological Sciences Research Council BB/M024709/1 United Kingdom Cancer Research UK C20724/A14414 United Kingdom Human Frontier Science Program RGP0065/2014 United Kingdom Swiss National Science Foundation 168735 Switzerland National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM 58448 United States CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Masiulis S Desai R GABAAR, Membrane, Channel, Nanobody, Megabody, Cys-loop, PLGIC, Inhibition, Signalling, CNS, Neurons, Chloride, Ion, GABA, BCC, Bicuculline, MEMBRANE PROTEIN, Antagonist Masiulis S Desai R Uchanski T Serna Martin I Laverty D Karia D Malinauskas T Zivanov J Pardon E Kotecha A Steyaert J Miller KW Aricescu AR GABAAreceptor signalling mechanisms revealed by structural pharmacology. Nature UK 565 454 459 2019 30602790 doi:10.1038/s41586-018-0832-5 0028-0836 0006 NATUAS 6huk FULLOVERLAP EMDB EMD-0280 associated EM volume
CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38.
 Human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. 0 1 2 3 4 0.33 Human full-length heteromeric alpha1beta3gamma2L GABA(A)R 1 1 2 3 Homo sapiens Brain Plasma membrane megabody Mb38 1 4 Lama glama Gamma-aminobutyric acid receptor subunit alpha-1,Gamma-aminobutyric acid receptor subunit alpha-1 Homo sapiens 0.05291660199999999
Potential signal peptide: MKKSPGLSDY LWAWTLFLST LTGRSYG FLAG tag: DYKDDDDK,Potential signal peptide: MKKSPGLSDY LWAWTLFLST LTGRSYG FLAG tag: DYKDDDDK
2 Homo sapiens LEVO MKKSPGLSDYLWAWTLFLSTLTGRSYGDYKDDDDKQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDI FVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDG TLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQS STGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATA MDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKS ATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ P08219 P14867 Gamma-aminobutyric acid receptor subunit beta-3 Homo sapiens 0.054444578 2 Homo sapiens LEVO MCSGLLELLLPIWLSWTLGTRGSEPRSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNM DYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAA CMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLK RNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLA LLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGI QYRKQSMPREGHGRFLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN P28472 Gamma-aminobutyric acid receptor subunit gamma-2 Homo sapiens 0.056922055
Linker sequence: GGSGGSGGSGK 1D4 tag: TETSQVAPA
1 Homo sapiens LEVO MSSPNIWSTGSSVYSTPVFSQKMTVWILLLLSLYPGFTSQKSDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNK LRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADA HWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQF SFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTYIPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLST IARKSLPKVSYVTAMDLFVSVCFIFVFSALVEYGTLHYFVSNRKPSKDKDKKKKNPLLRMFSFKAPTIDIRPRSATIQMN NATHLQERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYLGGSGG SGGSGKTETSQVAPA P18507 Megabody Mb38 Lama glama 0.057784300999999996
6His tag: HHHHHH EPEA tag: EPEA
1 Escherichia coli LEVO QVQLQESGGGLVQTKTTTSVIDTTNDAQNLLTQAQTIVNTLKDYCPILIAKSSSSNGGTNNANTPSWQTAGGGKNSCATF GAEFSAASDMINNAQKIVQETQQLSANQPKNITQPHNLNLNSPSSLTALAQKMLKNAQSQAEILKLANQVESDFNKLSSG HLKDYIGKCDASAISSANMTMQNQKNNWGNGCAGVEETQSLLKTSAADFNNQTPQINQAQNLANTLIQELGNNPFRASGG GSGGGGSGKLSDTYEQLSRLLTNDNGTNSKTSAQAINQAVNNLNERAKTLAGGTTNSPAYQATLLALRSVLGLWNSMGYA VICGGYTKSPGENNQKDFHYTDENGNGTTINCGGSTNSNGTHSYNGTNTLKADKNVSLSIEQYEKIHEAYQILSKALKQA GLAPLNSKGEKLEAHVTTSKYGSLRVSCAASGRTFTTYIMAWFRQAPGKEREFLAAMDQGRIQYYGDSVRGRFTISRDYA KNSVDLQLDGLRPEDTAVYYCAAGAGFWGLRTASSYHYWGQGTQVTVSSHHHHHHEPEA bicuculline methochloride 0.00036735199999999993 2 H0Z [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate 0.000746566 2 PIO singleParticle particle 0.1 7.6 Quantifoil, UltrAuFoil, R1.2/1.3 GOLD GLOW DISCHARGE ETHANE FEI VITROBOT MARK IV
Monodisperse sample
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 0.7000000000000001 75000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE FEI FALCON III (4k x 4k) COUNTING 30.0 1 489434 Gctf 1.08 PHASE FLIPPING AND AMPLITUDE CORRECTION
Relion 3D ab-initio
 C1 3.69 FSC 0.143 CUT-OFF RELION 3.0 Refine3D 30536 ANGULAR RECONSTITUTION RELION 3.0 Refine3D ANGULAR RECONSTITUTION RELION 3.0 Refine3D RELION 3.0 Class3D emd_0280.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 239.68001 239.68001 239.68001 90.0 90.0 90.0 X Y Z -0.32086563 0.4944583 0.0012150024 0.015643317 1.07 1.07 1.07 0.07 AUTHOR Synaptic human full-length a1b3g2L GABAAR in complex with bicuculline and megabody Mb38. Sharpened and filtered map (generated with Relion post-processing). FLEXIBLE FIT emd_0280_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 239.68001 239.68001 239.68001 90.0 90.0 90.0 X Y Z -0.03653343 0.081069745 0.00063416065 0.004324136 1.07 1.07 1.07 AUTHOR Un-sharpened combined map emd_0280_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 239.68001 239.68001 239.68001 90.0 90.0 90.0 X Y Z -0.04450547 0.0880118 0.0005448445 0.0049319845 1.07 1.07 1.07 AUTHOR Half-map 2 emd_0280_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 239.68001 239.68001 239.68001 90.0 90.0 90.0 X Y Z -0.043033913 0.09043549 0.0005497989 0.0049489946 1.07 1.07 1.07 AUTHOR Half-map 1