2024-10-09 REL PDBe PDBe PDBe 2018-09-04 2018-09-26 2018-09-26 2024-10-09 Cancer Research UK FC001078 United Kingdom Cancer Research UK FC001143 United Kingdom Medical Research Council (United Kingdom) FC001078 United Kingdom Medical Research Council (United Kingdom) FC001143 United Kingdom Wellcome Trust FC001078 United Kingdom Wellcome Trust FC001143 United Kingdom European Research Council 629829 United Kingdom Structure of Full-length Influenza Hemagglutinin (A/duck/Alberta/35/76) in complex with FISW84 Fab Fragment Benton DJ Rosenthal PB Influenza virus, Hemagglutinin, Membrane protein, Membrane fusion, VIRAL PROTEIN Benton DJ Nans A Calder LJ Turner J Neu U Lin YP Ketelaars E Kallewaard NL Corti D Lanzavecchia A Gamblin SJ Rosenthal PB Skehel JJ Influenza hemagglutinin membrane anchor. Proc. Natl. Acad. Sci. U.S.A. US 115 10112 10117 2018 30224494 doi:10.1073/pnas.1810927115 1091-6490 0040 PNASA6 6hjq FULLOVERLAP Complex of Full-length A/duck/Alberta/35/76 Hemagglutinin and FISW84 Fab Fragment Complex of Full-length A/duck/Alberta/35/76 Hemagglutinin and FISW84 Fab Fragment 0 1 2 3 4 0.3 A/duck/Alberta/35/76 Hemagglutinin 1 1 2 Influenza A virus (strain A/Duck/Alberta/35/1976 H1N1) FISW84 Fab Fragment 1 3 4 Homo sapiens Hemagglutinin Influenza A virus (strain A/Duck/Alberta/35/1976 H1N1) 0.035684851999999996 3 LEVO DTICVGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCSLNGIAPLQLGKCNVAGWLLGNPECDLLLTANSWSYII ETSNSENGTCYPGEFIDYEELREQLSSVSSFEKFEIFPKASSWPNHETTKGVTAACSYSGASSFYRNLLWITKKGTSYPK LSKSYTNNKGKEVLVLWGVHHPPSVSEQQSLYQNADAYVSVGSSKYNRRFAPEIAARPKVRGQAGRMNYYWTLLDQGDTI TFEATGNLIAPWYAFALNKGSDSGIITSDAPVHNCDTRCQTPHGALNSSLPFQNVHPITIGECPKYVKSTKLRMATGLRN VPSI Q9WCE0 Hemagglutinin Influenza A virus (strain A/Duck/Alberta/35/1976 H1N1) 0.022991572 3 LEVO GLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAIDGITSKVNSVIEKMNTQFTAVGKEFNNLERRIENL NKKVDDGFLDVWTYNAELLVLLENERTLDFHDSNVRNLYEKVKSQLRNNAKEIGNGCFEFYHKCDDECMESVKNGTYDYP KYSEESKLNREEIDGVKLESMGVYQILAIYSTVASSLVLLVSL Q9WCE0 Heavy chain of FISW84 Fab Homo sapiens 0.022925875 3 Homo sapiens LEVO EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMAWVRQAPGKGLEWVSFISATGLSTYFADSVKGRFTISRDTTKNTLY LQMNSLRADDTAVYFCARMRRTMIAFGGNDFWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSG ALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEP Light chain of FISW84 Fab Homo sapiens 0.023277766 3 Homo sapiens LEVO EVVMTQSPATLSVSPGEGATLSCRASQSVNTNVAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISTLQS EDFAVYYCQQYSNWPPITFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNS QESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE singleParticle particle 1.5 8.0 25.0 Tris 150.0 NaCl 1.0 TCEP 0.01 Azide 0.002 MNA-C12 0.1 octyl-b-glucoside ETHANE FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 43.0 1 C3 4.1 FSC 0.143 CUT-OFF 23286 MAXIMUM LIKELIHOOD NOT APPLICABLE emd_0236.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 551.936 551.936 551.936 90.0 90.0 90.0 X Y Z -2.234049 4.7277756 0.0032642235 0.17477667 1.078 1.078 1.078 1.0 AUTHOR Sharpened map of full-length Hemagglutinin of A/duck/Alberta/35/76(H1N1) in complex with FISW84 Fab fragment emd_0236_msk_1.map emd_0236_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 551.936 551.936 551.936 90.0 90.0 90.0 X Y Z -0.8879864 3.6798515 0.003264223 0.10987863 1.078 1.078 1.078 AUTHOR Unsharpened map of full-length Hemagglutinin of A/duck/Alberta/35/76(H1N1) in complex with FISW84 Fab fragment emd_0236_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 551.936 551.936 551.936 90.0 90.0 90.0 X Y Z -2.1590474 4.751162 0.0031178312 0.1312701 1.078 1.078 1.078 AUTHOR Sharpened map used to build linker region of full-length Hemagglutinin of A/duck/Alberta/35/76(H1N1) in complex with FISW84 Fab fragment emd_0236_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 551.936 551.936 551.936 90.0 90.0 90.0 X Y Z -1.4822797 1.9672213 0.0016271995 0.16696353 1.078 1.078 1.078 AUTHOR Half-map 2 of full-length Hemagglutinin of A/duck/Alberta/35/76(H1N1) in complex with FISW84 Fab fragment emd_0236_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 551.936 551.936 551.936 90.0 90.0 90.0 X Y Z -1.3434412 2.0703993 0.001637024 0.16683005 1.078 1.078 1.078 AUTHOR Half-map 1 of full-length Hemagglutinin of A/duck/Alberta/35/76(H1N1) in complex with FISW84 Fab fragment