Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit

2024-05-15 REL PDBe PDBe PDBe 2018-08-31 2018-09-26 2018-09-26 2024-05-15 Swiss National Science Foundation Switzerland Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit Ramrath DJF Niemann M mitoribosome, translation, Trypanosoma, small ribosomal subunit, 9S rRNA, ribosomal protein, RIBOSOME Ramrath DJF Niemann M Leibundgut M Bieri P Prange C Horn EK Leitner A Boehringer D Schneider A Ban N Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes. Science US 362 2018 30213880 doi:10.1126/science.aau7735 1095-9203 0038 SCIEAS 6hiz FULLOVERLAP head of the T. brucei mitoribosome small subunit head of the T. brucei mitoribosome small subunit 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Trypanosoma brucei brucei Lister 427 1.25 mS48 Trypanosoma brucei brucei 0.20181118799999997 1 LEVO MMRRVASTGSNGGHCYVGSSLWADSVYMEVSSITLQKRFSFKYATKLQHDEMRQPYYIHEKRYGIFSNERNIAKARRGLP FITPLYTKHMNLWDTDTDASNNRFFRGYYYGQRELHQLLGRPHSLEATNADGSNDLSTYEANTSQLYKGIPRPAITNLHY EPAWRYTLYQAGAHGAQLSNPRSPFTAKVLGDELMQVRDIKSVEHCKAWFDRLQYLINLHYEAVGDIGEFKSRHTRHVHE FFVAFHDALSSLDFRDTYLFDQFKAVRPPELSDLFGIFLEMEANYVHEDYCPRCSLPYSTTRYCGEGDVDTPFRKHRGRW APHQKWGREWYDVVARRAEALWYRATEDPYFGTPQHTQRQAEALLKVYVQAKQRGKAIDFMNKLRGSVEYLTGDITITTV MQESYDALLDTTPHPHLLTNGFTLESDAAKYTGEVNKAPLSPLQFRIDMEMNKYRRQQKEEGVVRVPPALWRLDTSAIVP YKVDSKTRRIVNWREVKEGIEKSFLSVGLPKEAYTGNEWREMLYLHDVIANREAKAAVLEQQQKLDREKLKARERASLKG EKSSNSASNGIGIIFPDKDGYQVFFVDEASLRPFGIGASGTLFKAVARVYPSPAAVPYDDPVHGKQFLIDTTDETCHLFG GFEHGDRLLLRAKSSGGDVDGGNNSNCRNTGSFTDEEEEVIVMGVSTEGSDAERMLCAMHVDTGKQRERGIVFLGTDCID IRERWESVRYAASAYVKGRVTLLESQRTAREEFMGQVVGIRDGVLFVQWRLLKGGGSVLDRSVAVPIGDSTQVRELFVET KVLGVEPLQSPPSWRTPFRNDFAEERLKELQRAPFKREKYVSLIQGKYTPKVKKFGYTQHTTVDDFETKEYKDRLLSKQF FQNPQAFEVVPDRHERSVQFGGKWEYQRTHGLPTVDRNELENGWSEVEPISDGEMKVIEQALRDISGPRPGNFVKPPSKT KSLQLSESWWEPLGYGWEQHNEEQKALCDVTEQRLIDGSNLPFGGKLPPFGTSYGMGERMRAIIEDYSKGFGLGPHGHSP THDTIHYNTLNAEGERVRDLGYTDALGRLFSEKLGDRDVHQWAVESCADGEADVRQLLLSLHEWRERGRPPSLLLANVLS KYLEEEIAAFNKGIPENAPKLQLETSDGTLAHSGSGGSRSGTMWADVDPTTFALHQASQSTRSTRCDEPFILHLVKRAKL GECVTNFTDTAYIAHLESSVINEFHLALTKLVGKGISPTLLAQKTGQLHRGSVRVGGNVVPFVKSRELSRLLERMGLTSE SIAVITRGLANCPEQESVGDDFAVPVSLILSWDGPGSSGGSGSGDRRLNTTAARNEQQRKGSAALSSAIRQLSQHQKQRG SASGRGWQNEDKMVVHVLEELSLRNDGLVMDIQYAIRENKKNRRLRWEFVTTLLPVFGGNEAKVEQLYSDYCDGKYVPNI TVAVEAFIAFLHNATQHPETYSASDYFDIDDGKSSTASGTGDQPSAGQYTTLKLLDPLEGPFVFDNVKVEYIQTVERFRR HGIRAGPVMAPATGFIAANCKSLNYFTRRQEEVVYVTNDSDQGLRRSLENSAYHKTIAANPALQYLLKARRGAALVETFN RFFYRTMPMLNFYQNVLKHYSETIQPLRQAAKSSTRGLARALESERSAAMEEFRRNSERYWRNIIEGRSVEQVVLANGES NRRTVGGGAGEQLQKPQGNRDAVRTNFTGEATDGSRKGQRQHQQPPLSQQQQHSRGEGERSAVHTSPRQGGSRGMADLLS KLNTPKTGSGVKGSTAKNTGNNRRGPKS Q57UJ2 mS59 Trypanosoma brucei brucei 0.035395676 1 LEVO MRCSCAFLDKSVFAAKRRVIVPIHPTPNFPAHFIKSAFTTDPLKEKQKARFSSGGEAMREVQDIPKNLEGERSRRDLASR GDTEFQALVEFIEGASYDQLISGRRFKKVYDVLSENDDMFIWLCHTAMSVLNPGDMRSRLIYNHLRILAESVASGEMTQR TAFRFFESAVRSPAYREIAKRQLEGGAATRLAGISAAADVMRRMGLTRRPMSSYFELYQRIVERSEAMTPWGFPPLFQFE ERLSLEPRLKFFSRASQQSLERRRRGHVMTPHTTLHGRRIFWIPPTWNRAGRFLGPHVTLYPGMTPD Q38BS2 mS49 Trypanosoma brucei brucei 0.13723412499999998 1 LEVO MIRRRVCDGARLAPNIRATFSAVRYQSGLHTFIRDSKPSNFSSVRRSENANGDATASPGATAGENPASSGDWASHMQREL FGEVDPLGGQAHKDYYRDVTRGYSPQYAPRNFANGGAVAYPHIQSPYEYEEAAHRRVWLDHDVDRMREEFTQHRASLRSL ASAQEREELLRSRAAEYQVANTVHESESVHPIQQLYNSGGTSRSALKQQAVADRYSIAEQHSPLPLTTGVDRDALDEAQR TKDRILNDSFTAENLLITHGLREKEKHDFTILQRTVRIPFQGYDMDRFLAQQKGTPYGAQQLPPNVVPSSMEEAQRTLRG SSATATPLVDAVAQKVYARNTVVDRPAIGEQLTEQIINIMRASRTTAEQQREEERAQRFGLGRQGALVQDGGPDQRTLKK HTNDERIVDAMLFQQNAYRKTPTDEHWNPYIRRSTENGVGHLLQNKFDIMRREDRLSKGEQDLTERNTIHYGVPIQQIVD EFVFRHRNARGERPLDYFKPFPNFRALRLNRMYRDVEGFSLMKQRPEFLEWELFTRYRQHHQQRRRLALLHGLEPVANET AQERDTRRHRLDEICERTPFDEREMRVNDDEMRVSVETLRSWFGVYMLPSPTVVNAVLGGSASVNLHLYHLADEMGTADT REHVLSSRYLNRLLLLESYQNRVGRGFMNHVVGRAPEPVVPHEQPQEVLRHFSAEERAMYEQHVKEQTSRQLGEWERAMK RRRWLTDHQQYGHVVSHGLETSVVDLSHTETGAVLTVSTKAYEQEIEAVRMKTNATIKVDGMVYNLLPNSERRVVPLTVQ LDSGEKIDMTSEDFDRCELEAFPRNLNHALNYGIANYAYNRGNYVETQDSIWEEQTASGQEGWSPATHADGLREGLPVRA RRPIFSSSAEQRIAGGPQRAVIIQYHHQPFFNPEPRLVKVAFQCDGTIMEVPISDVMIWQRRYHGPERTVGDESRRYNPA AMRRYVDVTDPFNEKTSNTEHFLDKYEPKRNADTVADKYRTTKQITEIDKWTRYDSARADNYRPLSISHRRDYIRMGYIP RYTPWEWIAIQEADQPLIAEQIRQDNIGTSYFFSLNRYWRYKASPHGYIRHFENEVRDLLQYVDGVTPWKQAQKIRTYWE VRSHHPMPQFNRPEVAMHRNTVGLLPAHMWETDKKTGKVKSVKDSVRDYQTKTPYPKWVQL Q586P5 mS50 Trypanosoma brucei brucei 0.13171924999999998 1 LEVO MFRVRRLAGFIQCSPCGKMGPLKQQTRRYTPIWKSDPAVDNVAPLRDEDERRALWAEVGPISDVGSAVTAWIRFGNDPVL HTAVPTMLGGKFRNQQREKESLLPNSSSPFAYVEDYMGTNLVFGSPVHAKESAAVWATYFERRYASRLRLSRRTVANYVG LINSPEVFDDESDRPETRWSQDTFFRECAYLSEKFLKEKVSNMQQFEAALKRASPEAYLAFFDAFQQQTQTQIPLPSPSV WHYEGERRKQWAEKFISISHKAQAFFKDVLSEDVKKYQEVPGKLLQKVKPVLADVGKILVKRHERWLKGRVWTSLTEEER EAYCMKEVKRQQMQVEDGEFDPMMEDDVDDTELEEWQREHDAIMKLMNSPIDGLHFTTLELWLHTMRCEELETEHIYTSA RVRAIQVAARKKLYDTTSYEEVIQAVVESIARGTLDLGAGVLRPHFNEVWCQLNYAKFGSSTITQHTTTSRRQLLFFHAG SLKDIAATATLYYATKPLSNSLDYASPYKYRRSLITLCSNYGVETAYTTQRPLLRSAANLARAEDLIHAVVTAAAQPFGE RRRAATRDLHMEFQRLAVPVERVIVANPVSALLESGADPDEKPVEGEKVNMWPLGAKRVVLYKWSAPNVEKLKAMESDAA SAVSGSSLTAKRLREIQELKRRGFLEVSLWRRVTAQERKQRNEIVEAKKKQVEEVVRTVPSLAHLHQYATSLYSRIEERV AFPTETSTVTETTNMKEETNKPLEDSEWEFAVLLDDRVLLNKEESVELYLPYRDANGELLAQGEYRALVRAFDLEANPNL HPAYCSVGYSESFQVFDALPQLIAQFFRVKDATAEAAGVTHIPAADFTPFCAFLRDAGLDVPLRCEFEAGQAVTTDGDVY MDYFLQLLRGEAFHQSHAQAGLTEAQRAIEPLCRAHWVVHHPGADESEWATARRSVLDHAMQHEREWWFPNEMLDVKDVV TGSTNGLTPQMYPAAVRYGVELCTVLTAEGKFVDERGSGLSARCVVNGTGAAESVVFDTANCNGTNTTSVEDALRVAHGA LRSAQDRHNTLAAFRLGPLSKQSQVLLFCGVNAYEFGGKYARTYAYAFEKAKKELEATAASGFMAPSLSHEDTERLSDQP TTSPSVDRFASTTHPEQRKAQFVPRVGPGSTPLEDPAADQKSEWS Q57YB5 mS52 Trypanosoma brucei brucei 0.085012258 1 LEVO MRRCSFASTCRYRSADDVPLGLGSAYVWTCRNITSRGQFNPIHNFSYAMERGVRARDVKAFEKLITNPGPLRVAYTPDYL DWLHRCYKAKGTYMDARAVAEKKFNGNIVSSELSAAVNRREGKRGDTRGDTVDNDHHNLPGAPPPGMFLRPAHSFRRLAG ELKRRRAQSILDEVARAQGMLDLFERQPHFPAIHIDRCSRFHLVELFKEMVLERSLDSNMIWEKALLYRAILSERKPSYP TSFHYIFTAVEDTVFAPTISTPMEMAGSGGEGHDRSSHPLAAKCPTLEAYYYYVYLVKKYYIDNAVEAHVVLRCHREPNA ADLLFSNPPPKDDTEIMKAVELLRNADIQRGVAAAAAVSDPTLPPGGEGSVIGNSDNKKNSEKTSEGSRGRPARPPVLPG AYPPIDMLWRCEENLPLLKVLLFGEFNLIVSENPFVKFPSAHGFLTRPYSTDSSRTLADGMSLANVMAEKRGHLLPSLPR NTATSIDARAQDIRRLQQKHHRDDIVSFQKLLRTTHAEDSPSAFSSYSDWSYFNPRAVRAEERDRLTRKAVEALKLYDSA TNDIYRHSFEDVQACHTQRVTERDRTMPPYLPTLPHFVAIIKKDPHISFLLHIGLPDRNSSEEGSAKHKELEKRIYYLAR ALYHTALEYHNETVRRVNRQKVNVAASLLDNFVEQEWTTILRDKHDVTDVTKTLNDTQNDKKQLARRLGRYMLFANRSLD DTGFPTDARADDYTRWMAPPSVGKVSL Q386Q7 mS53 Trypanosoma brucei brucei 0.075338914 1 LEVO MSVSGVFSKGRGIGHEATTSILRYIPRARVPWQPSRFGRENLTAADMARLWSRGRYRDGPGNYNSGYCTERTHVLEENTV SIIPRRELEKYMPDITIGPKALVTPVSLMNARNGHRVTHDLLHSYDPHIGRLGKPAVVDHDNITVEDPNRVGLNAATLDC RGRIYRWLRRGPFFQVDNYFRRSVKLNRDGTLPTDFVHEAPLMRKIIRLAHRGHLKAACEEYRRVTTVPPVEVYRALTAC CVPGAKLADAVSIFEDGDSKLFYVSRDGEVLHNLMRCAIAARHRARIMWVYNVMRGRFYENVVVRAEVDLIWRYRIAMIA LEYLLDHECAEEAAAIYSYLVEEELLRCDVHVRVGLHMREAIAAGKPITLNNDVMNATSLVRDATAVAPEVARELQRRHA QTLQNNAVEAVGAENVREGSAPWSILGPLTAIGPTAEDTMVWLQQHYGDVDVMSIMRWARFRKGKDLMAKDRPQYLARAA AWIELLSKRNREMEEVPLTYMRKSKPLVLDTNSNVRVAWQTPLMRSGGPPRLLAREEGYVFHHSNSSRFVEETYRHPGES LQSRYLALQPLHTEVSAKEDFQRLYYQAQKHHKQQERLLPVSTAAIPSRIVHHSVMSALHGVSGKGEPANRSLFT(UNK) NKSNDSHSNTGVASGTSACTDGAGSATPEF Q38ET1 mS54 Trypanosoma brucei brucei 0.072046031 1 LEVO MFRRAIPLLSANIPRSVWDPAQHNPNWSDSYGHDITNRRAWPARKWTVGLEPCTPREWLQFSHRNLAYAYNGALRACHSL PSMLLLYKEMKQRGVKVDVDTMNVLLTRAARHEHIQVDDVFLLFDELVALGARPDLAAAETLHTVLSHSASMPEEWREAR RLQLVELYNNLAMEEVERLAPHRADRLLKEQMKRFRGNLQQLGSGLRPTVYCRYLHTTHTAAVLLEEVHNFLWELVPNDH PAMEIPALQLRVPFVASVLRRPSVNPGVSLASVSRAEFGDTDVCAVFLAAAERMVDADFDDQRPVSERRLFLSLLTMISY SGVLYTSDLMAQLMEMVKYSNNDETRDSDAQRVLRYALRGSSAAQDSASRTLWHSVEKVADCRVVGRYIGARNPWNPIRV CFDEQGVFKAYPISTTTTTREVSPPEGNGAVTQEQRASCVEGRTLEALNMRWDDVRRLIECTGVLVTPPSERCPQQQKME VFTGMAVYLRTVATGRRYEGGEDVLSDGAVATSSCEQRRRGTLFAEGYDFDVWVRLFSLVQEVRHDMEKFMADHTLQCVE PEFECWEALLVTLRCALDFCVVQMQGGGARGTEREVVERLFRDVVALREELIEESRTRFGGRMRVLWLQEA Q57ZP8 mS55 Trypanosoma brucei brucei 0.06667667999999999 1 LEVO MLSQNVAKTTVPSYYMIRTNLPHRKPQNQWEGVYYYSGITKRQRHLILLHRKREREAHMRSFNISRASVLQRLEQLSGDR KQESLPPHVRLDLAVRLAQHGLYQQATPIVDELHHQKALHAGHYALLINALACPRLGQRILHCDAQCDPALTYKLLGDEN GEERAQEAYRWFDLALTSLAVDCGGRTQPSHFVPYLPQGTAAASHITNALMRTLLTCGYTHVAAIPDSVYDRMGSMGISP TISTYELVMLALSLQGNMVEAESILSFLRSHHSEHITVESFNALLLGHREARQFDCCDAIWQELVDRRWPRASPLTAELY LRSIMDHANTPTSEPLQSFANINVVEKKKVPLVLAQMDELGVPRTHLSRVLMDEVEDSLRKFQTYRSRFYEWGRAVKQFD FIEFRRRNGWLYDLHLMKCTTKQVGPLRDFNDPDAVQGAVATAEIPAFFNERPAWERPPLEETLYVTTNKERYDDVRGGD IYYDDTRGLHDRSPTWMNEVPETRYDRLYGVNHPDIAKIGIRRHLNVEYVNRKEVVERDAALMKKTLSSGRRLRHRVESS RTHRNAGSLSGISSTAGGGSR Q580V1 mS57 Trypanosoma brucei brucei 0.045956292999999995 1 LEVO MFRSTRPRSVGYTPVNPDTSPMVAYSQYHWHYNLPQGMERPHSVNRTFAAPFQSNHSLVNKYRGVWIEFDMHPAFSVALE PQLRKLPRGRTLPKTPAEEVIADYTALAPLVDDEKTRDLWLAKVFQHCAFQRCGGAMELWERYCHQRFTAEGATAKPPLS LVKSVLFYCNKTDNSGWRALFDRCLKDGWNYTPLFDTAQWSFMLKSIGRMGDEDGVRAVLEEMLDVQADLDRVEARSVVI ALNAVTNADVYEFVKKYLFNFGERKVKFLRTTYSDLRGHGAGKLRIPLKENDNMYYHVCWHSSIRSPRQFSPRQLYFDYT PSTLGSSSHNPNAKIDDIVKDKIEKWKAEGLLPEDYVHEDRVYDRSAAFKNVARQEKWKKMPKILKSKRMGYTGDP Q584U8 mS58 Trypanosoma brucei brucei 0.03562391 1 LEVO MSFRYTNNLIGALKHRLLLESSYREIASRKFIGNCRGVEVVCSGYGTVLAVQLTDKAVWESFYRKGGRPTVSGGGDVSGD AETGTQGSATTTGATGSLDLDKLAESIKTALWDATRKIRSAKEAALHRSLSHNTRMRASADLKHWYEEDANTLRPLAFEA LKHEAATPWMQLVQHGKKEEAAALLKEFEQKGDAAEATPTRVKDDRVKGTRTELSNREQPPLASTLKAEDSNPATIPIGS VHPLFTPALVQIEEAGGGSVSNEAVCRAQLWELSRDEQLFWERVELIRKGQVASIGSSHKRGYADEAAFAKDDTEEKVQL RFTQ Q38BP1 mS67 Trypanosoma brucei brucei 0.030096051 1 LEVO MLVSYLRLQADRRTRHHMMVDGQINPREWFNWHPKEYQPWYFDRHKFLYEQRQYAQFHHLLCHQVYLKDMLHLMRYPEPY THIIDCRTSTQKMHRWVPHSLWLPRDEVEYALQLSADEFLDMYGFRKPDKSDDVILLSHNGIYSEQAGWEWKKQFFQHVY NYRGGTNELFGESYSDMNVSDVLSPWKGPFPQSGVFVDKWSKRKVLTRTGPFDRQYEMQDFALPDLELEKARHPEEGPRQ NMPFGLQ Q586G5 mS69 Trypanosoma brucei brucei 0.021589732 1 LEVO MRRVRDGLFLSHPSSALQCSVAVVSTGEFDHPPFQFRQRHTFNTTPLHDANRFGGRTAYLREIGPVNIKKQGRRFKKDPR TVQFNVDVWCAQQTLRKRWKQRDWEVIEIPFRLVPREQQRVIPELYTDIPQMTDPARNDFSNIRNKVYDREELQGVLFPA AGAMLYPPLQRVDKQAMTLDKYL Q57UZ6 mS70 Trypanosoma brucei brucei 0.021247398 1 LEVO MLRFTHRALTATPERFSVLGTTHPKPKRTGFGRNNKMRSKPSDNVAWYDKGPVEWLPRPVRLTYDHLDQLQQWTMRATLD GRTEEFNRIRDLHREWSQHPLMPVLGDVEPKFPLNLFKQNHRAKKRFLVRWHKANTPANWLWMPRGPTVVTPLHRTNPTQ YPENWKQMVQRKSGTGTPS Q383N9 mS71 Trypanosoma brucei brucei 0.020093226999999998 1 LEVO MFHRAFVSSSDLTGCTIALSSVCTQKRYWAKPKKRPKVGQGFHEKAQKWREEYLLDRHRALADSLRAYVEFSTSKRVEPW DARFKPFDRIEKDGVYVLMRYMMEEKLQLCNYHHRPVKRLFCNIGLMGPQITTKARWKPYRFATNPAGTSKAERMYQRDK TVYTHGHND Q383G5 mS72 Trypanosoma brucei brucei 0.019317293 1 LEVO MLRSTLTSLNTFLTSSVATPPISVIRTGPKWWADPERMVRQKLMYFTLGVDQLPLRRTAVIQRDLQRFHMCKPPPRVGDS TGYKRSRAAQLNTWYRRIQYQEYHMQHLFTRHVWGLLRVYPGNTTKIQGKADDGYVGYDSVPFHRYNRAPLPFPARELYE RRK Q57YD4 uS3m Trypanosoma brucei brucei 0.00907094

Mitochondrial gene uS3m (MURF-5)

1 LEVO MFLIHFVHYKTILQKYTFKFKHIFLSIDKYNSLFFNISGILIWLNIIHINIILIKYSFFILINNFEYLIILIST uS9m Trypanosoma brucei brucei 0.050128926 1 LEVO MQKLLSPRTARHARLFRLAGKLADSGSPGVPKSDGERLVWVNSHVRRDKDISLSQEEERIRELMMPLEVGENSFAANGQA THGNLFYFREYPMYPGEYVPAEHNTLSSLRDELRLDLTAQSLKEAWMRVSGGVYFQSVDEYYASVDGLDAEQIGEVLAAL FPELNCYEAQALVQRTLECISRPVSAASRQLSRTITAEAVGLDNAPGHYTNFLEWMGRLTETRAFKTEHALFEFSRRKFN RDDVRVMFENYRLMSKATLLADSADSYSHFYTVLKDFA(UNK)KVAGEDSRHQIGVRIDEAEVDPETGIAVGRGCADGEK YHFTALLRENRDHNGIITVMGKPLSLVLDNKAWLMEMVLMPFDEANLDYRDFDAHIVSEGHAMPSIANEIAAFALRMAVA NALVKLIPLTRIPLKKSGLLSVDRRRERGQFPGYLDGKKVKRRFAKR Q57W62 uS10m Trypanosoma brucei brucei 0.094351039 1 LEVO MVRRSHVVAYYWSRYRMPTQMPKFDGPAPVAAPQSMNSTKTNEFIDPIDDKFPMSIRGPLVRPDVPEDQYVDSWYICTSM THHMGDYRPWSASAPPNAFRFRPFNEFDAKGREYVQYMREFARFDPRKSRGNGQKGFPFRDAYLTKMNEANQKTPPPTLE TIMDRAVREHHQHARILSPLEVQRDVGRLEPIPSYAGKINADRSVFPFQWKTEDWYEYEVAKVRNRRFVFENTEEDGIRG SEVTYKIVLEGFWDHHVMKLAEDVCMFLKDVGRQIVEEKLVAVRRLLQGGAVDPELLAAFNCARAGPFGGLDEYDKEEVA NFLRSDLRRLEEQCLSVINRCNVPVPGATNIYDPHTSWPHVEKLEPWVRMAEFWTSSSDTSFTELEMSTAHYEFRKFFRV IICKLPFQSTEFEKRMYDIRHWLHRQTSCEFHTIYRRNVIHDSAVFPTEHDPATPTTHEHHRMFSFALDWQSAPVNRLST DTVHEGESWDAVAQRLGCSVGELKDANAERETIEAGVVINVPVTATRRLTSFGATPLVLPLKTTSAKDGERIRTWEEAAA ILDCTVEELQQCNGHAALTYQKKESEAGEFDSSETELVAPLSCWTSTSESEFSPVERVHANDTLVAIARRLQCSEEALRA VNDGITDVSGLDFVRVPPEARRPRRLVEPQLRPQAATDALLARTIAEEETFKLKSIPHLPQNAERFPHEYHTPTSRFPPT PSETPATQDWMAYTAKYLDKQFTISAEPAPVYNVNKLWPMQQIPGKVDQTPFEEDQTWLLHSIPVQQLEMHHHEKDLQDL PFINHEQFPRSLEWNAP Q57Z45 uS11m Trypanosoma brucei brucei 0.03792591 1 LEVO MMRKSVTFLRRGKPRPRAGMFPDKYRRVPMLLKPQQGGQQYFNHFLIRSTNDRLTQQDVDNASGQAHSFISPQLPQMDWR NMSARSSEESIREEMRQLAENDVMQHQRVFNERMWYEKEEEHRMKARSCPEETSEHA(UNK)SNDGAVPPPRVLGGDYFK TRFGYSLVKNSEMTQGPVDYSQLDMWGEMPRYTSDMVFLYLVSRRRNTYAVAYTYEGKRILNTYTAGNRGLKGGDRGFRS EGSTDNGHQVTSMYLNDLLPKLREMRASEGRPMGRGEKVELVVRVMGFYNGRQGAVRAVQDRANEFHVRYFEDITPFPLN GPKMPRGVFK Q389T7 uS14m Trypanosoma brucei brucei 0.019900013999999997 1 LEVO MFCFSRGLWMRHIGQDVPKRHTHFVLESRLMYEKSFRDCWLHSVCRAISQLDEPLSKTVVGTHQKMLQRKVTCFQYNQYG LFKTPYYRLANVDRYHAVQGVAGTREWVPYVNVSYWTMNKMVRGGNLLVHRVHYTGWGTDSHLKKGGWEHRWNKVLQRNV LQYSRI Q580I0 uS18m Trypanosoma brucei brucei 0.036988691 1 LEVO MNRTGGSIYAHALSQFAVCRQPWNEYIGLLTKQDSTPYHTEPQEKPAYRGRKQGREGWLFGQQVQLHYHRFPDEQLITNL SRWRTGETVGDIAMQQFRNAQPFDIEDKDPQGVQRPSPEVYMKLNYKNPATISRFLTRTGHMYPADILPLNPEAVVKLRV AKAQAIRIGLYPRFGNPFWFRSQKFRPKAYQENYDPTTYSTKRTVEHFAYNWVQTDRIRRYFRELEAVHTSGSASARGSG GGTTAEHKQQDQFYSPENQPISLHRNNISYMEDVGRSVKNPTVPGLMSTKGMKKKFHNLYSSTSTKRMGFSNPTLGIKKV Q38AS2 uS19m Trypanosoma brucei brucei 0.028364603999999998 1 LEVO MAFRNTFTTPGKFSTVSKNIVLLLIWRVKVFLRAEGFAHSLVMLPVSLYSKILLCDVKKKIVYFHCCTRKKSMLRRCPCV WPDGPTKSSVSIGTAFTLQKRFLKIAKSAFGFYLARRGQRKYPFLRRPHIKNTHSMNPSAPYFWSFMTAKSQMAFLPEEN YITGDWTGKFFVSKRQVYTLQHATSGAKVRVKSFPSIFEFNSPSRWNIGKEMNTLTKPRMDLIDEQMLTKKQRLDYVRAG LLPK Q584T8 mS29 Trypanosoma brucei brucei 0.057048836 1 LEVO MRSKTLKKSLAAMYMRPPVTCYTDACEAPVAMWDGAIPLKETRKLKNGVPVRTVSRTYSHPPQLTPTQLSFNDINSMYCV GNDELIQFFPEGLGGRVFQTMPPGHPRGFLYRKETHLLNLFVDKVQHWHTKRSVLSSLTNGRTGFIVDGPTGCGKSALMC QVVHFARSRNIVTLYVPDAKVWTHGEWCWPSTILPGFFDAPDAARSFLKYFAVANRATLTSWKLRCTPKDLPTEQGERQP QNLYELCEWGHRAVAPASIDRQSVCVKFLMDELSEEKKLPVVIVVDGWNLFSHETHFRYPHPDFLRGLASFNESSTDIDL YPQELPRIPASRLSFVRGLNKMILSGDDPNKFFITCTTRDFKPFDGISGFPNVETDRFANSLDEYAPYDPEKDSHFHPIQ IGNFDEYEYRSFLRFLINSGELAGLGWGPLWHASSDFERKLYKIGFLSGRNPQGVVDHYHQELVWRYDYQRTRQKQYLLK RRMEGMSRGASSGAVGVR Q585C2 mS33 Trypanosoma brucei brucei 0.021244104 1 LEVO MVLRRWFPLLGVHRVGYTHPSTLPVPCAQRWDLRLARARIFQEYIEEKAPGAWQLEDERSMSPEFKTFTGYPMRDMRPGY GQNLPDYIMKKRLPNNTHYELFARRDIPNEDNAMYGKYLYDMTVHGTSLPSTYRMHKDINKAQRNDRKLSGNRFKVLCSS GAKNPPSGWEPIPDATEEEED Q57WW0 mS35 Trypanosoma brucei brucei 0.098377773 1 LEVO MRRWHRSLNPALFGERGSGHYFSTTRARLQLQAQDRIICGTHGAGIVAHQTAVNSSDNPMLSSVTTAAPAADLSRTKPHE GTGTSERDPYIRTLHNQRSAAPESSVSQSHTVNAPTVDECEMLAERWGTMNYWHNDTFPRLVVFLKKLLVPDVSPLSPTA ESLLSMFEKVVIPKLTSDEEDRRKLVSLWSETTLQAEAAVTKFLFQRGSFESMLHRIITDALEKMSTLALGGQEGNLALE ALKRQTLFKRNDYIQKRLIDVVSNSAYLGYGDSVWQVFFAAVEANEENLLSDRATTDAIRAAWEGVMREDVVRLPDVTGV VALYLTLVCIRESGRLVPEELKELSSGLEDGVRPGVRKLQQYPLIFLHPTVKRRFVVKAVAEILHNSSSNAFSNMLRENG LHDTAREVALCEAMNRNKELAEGDVGDAVGRFVSKGEVKTLLSSLVSGTDAVVRDAVAGIFGIGTTITIDWDAVMQNVDW SNNWQRLATALLSNSAVLSAIVKLVKNAIGAKGMSKHLFTDEYADQLQLILDAREERAASRKQRIENIAQELSSFERVDL SCDLLRKLGVDMTELDTAAAATRNMNVVQRPCIEDGLLSLVLEAVTKRHPNWVKAGVIQTTLKDPFDALRWMMHIFIRLS YVPHAGAATIARLSRRRIGPIGLEPHQFNVPAELGFVEQYDNLQYKRYDWQGWYQRMLDVHNRNVSLRCRICDLQRLDGN GVQFVDMQTERRLRILAQHRVGMGVLKLDADKYEDQADNVTFGTTKLSELLADARKAQLGEEYWPSVELKVRKPSGQSKA HYSLIDNERIEKRSRELYEKYRDAKKRSLFVTPMETWLEVKGMQVRKSVDNADEDGYTLDALQDMMDGDDGDKV Q387C7 RNA (143-MER) Trypanosoma brucei brucei 0.195379688 1 UAAAUUAUGGUCAAUUGUUAGUAUUCAUAUUAAUUUUUUUAAAUGUUUUAUCAUUUUAUAAAGGUUUAUUUUUGAAAGAU UUUUUGUAUAAAAUUUUAGGAAUAGUUAAUAAUAAUUUAUAAUUUUGAUUAGAUUGUUUUGUUAAUGCUAUUAGAUGGGU GUGGAAAAAUAAAAAAAAUAAUUAAUAUAUAUCAAUAAUAAAUUAAAUUAAUCUAUUAGUCAGAAAUGGAUGCCAGCCGU UGCGGUAAUUUCUAUGCUUUUAAAUAUUAUACAAUUAUCAUAUUAAAUUGUUAAGUGCUGAUUUAACCAAUAAAAAUAUA AAUAAUUUUUAUUUGUUUUUAAACACCAUUAGGUAUAUGCAAAUAUAAAAUUAUAGUAAUUAUAAAUUAUAUUAUAUUAU AUUUAUUCAUAUAAUUAAUAGGAUAAUAUUUGUAGUUUUUGAUACCAUGAUAAGGAUUAUAAAUUGAAAGUGUUAAUAUC AUAAUCAAAAUUUAUUAUUUAUAUUAAAUAUGUAUGUGUAGAUAAAAUAAGAAAUUAAAAAGGUAUUGUUGCCCACCAAU UUUUAUAAUAAAAAUAACGUGCAGUAAUUAAUAUAUUUAUAAAAAUAUAUU M94286.1 Unknown protein Trypanosoma brucei brucei 0.00044353899999999996 1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK) Unknown protein Trypanosoma brucei brucei 0.000613749 1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) SPERMIDINE 0.000145246 2 SPD URIDINE 5'-TRIPHOSPHATE 0.000484141 1 UTP GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP MAGNESIUM ION 2.4305e-05 3 MG water 1.8015e-05 3 HOH singleParticle particle 7.4 ETHANE-PROPANE 98 278 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) INTEGRATING 40.0 1

initial model from 2D class averages in EMAN

FOURIER SPACE 3.08 FSC 0.143 CUT-OFF RELION 101308 OTHER MAXIMUM LIKELIHOOD emd_0233.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.2582923 0.5873829 0.0010537558 0.015823135 1.39 1.39 1.39 0.01 AUTHOR ::::EMDATABANK.org::::EMD-0233:::: map of the head of the T. brucei mitoribosome small subunit AB INITIO MODEL

We used Coot and O for initial model building and refined the structure using Phenix

45.0 emd_0233_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.03645227 0.11290482 0.0008065612 0.0067006936 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-0233:::: half map (odd) of the head of the T. brucei mitoribosome small subunit emd_0233_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.03550182 0.11861558 0.00076657766 0.006699278 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-0233:::: half map (even) of the head of the T. brucei mitoribosome small subunit