Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit

2025-12-17 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin _em_admin.last_update DERIVED_CALCULATIONS atom_site ndb_struct_na_base_pair ndb_struct_na_base_pair_step pdbx_nonpoly_scheme pdbx_struct_conn_angle pdbx_struct_sheet_hbond struct_asym struct_conf struct_conn struct_conn_type struct_sheet struct_sheet_order struct_sheet_range struct_site struct_site_gen _atom_site.B_iso_or_equiv _atom_site.Cartn_x _atom_site.Cartn_y _atom_site.Cartn_z _atom_site.auth_asym_id _atom_site.auth_atom_id _atom_site.auth_comp_id _atom_site.auth_seq_id _atom_site.label_atom_id _atom_site.label_comp_id _atom_site.label_entity_id _atom_site.type_symbol _pdbx_nonpoly_scheme.auth_mon_id _pdbx_nonpoly_scheme.auth_seq_num _pdbx_nonpoly_scheme.entity_id _pdbx_nonpoly_scheme.mon_id _pdbx_nonpoly_scheme.pdb_mon_id _pdbx_nonpoly_scheme.pdb_seq_num _pdbx_nonpoly_scheme.pdb_strand_id _pdbx_struct_conn_angle.ptnr2_auth_asym_id _pdbx_struct_conn_angle.ptnr2_auth_seq_id _pdbx_struct_conn_angle.ptnr2_label_asym_id _struct_asym.entity_id STRUCTURE_SUMMARY chem_comp_atom chem_comp_bond database_2 em_admin pdbx_entry_details pdbx_modification_feature _database_2.pdbx_DOI _database_2.pdbx_database_accession _em_admin.last_update DATA_COLLECTION em_admin _em_admin.last_update PDBe PDBe 2018-08-31 2018-09-26 2018-09-26 2025-12-17 Swiss National Science Foundation Switzerland Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit Ramrath DJF Niemann M mitoribosome, translation, Trypanosoma, large ribosomal subunit, 12S rRNA, ribosomal protein, RIBOSOME Ramrath DJF Niemann M Leibundgut M Bieri P Prange C Horn EK Leitner A Boehringer D Schneider A Ban N Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes. Science US 362 2018 30213880 doi:10.1126/science.aau7735 1095-9203 0038 SCIEAS 6hix FULLOVERLAP EMDB EMD-0231 associated EM volume

large subunit of the T. brucei mitoribosome large subunit of the T. brucei mitoribosome 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 Trypanosoma brucei brucei Lister 427 1.9 bl27m Trypanosoma brucei brucei Lister 427 0.021793092 1 Trypanosoma brucei brucei LEVO MLKCSLCYLASKVTAGNAKNQAGHPRRKAKLFHVVPGTPVTPFEKLKEQRRRFGQDRHSRLPEYRPGNNVRMDPNTYTLY ATKKGVMTIRESRINPKYKWLDVEPDIQKVYRSRELRRALQEREMASMAVGENSNYRVELDLLLEPDWRERVMHVPKATE RFKDPNLFTRGVVNELSPLDRYSYT A0A1G4I8T3 bl28m Trypanosoma brucei brucei Lister 427 0.028051372999999998 1 Trypanosoma brucei brucei LEVO MLRNTDVRLFSTFALNPETSVAPHGPPRGLVNRYVSMGLPPWAAWCNKVNRYSLYRMSGVTQRSFLPKPPQEMDVIWLNE RVRERVRTSRQVQNVYRQLKYPYVKTGIHYSDVLDHWVQVPMVEAAMFEVEKDGGFDNFILKRSGPELRSTYGERIRRHI LVRQKEIQKNFVLQKQAQMLVESMEKEILPMEDGKKVEEVLEKYGIDKEQLLRDIARAAVAKKQQLASAVPQPSHVEVST N Q586A2 ul29m Trypanosoma brucei brucei Lister 427 0.054045367 1 Trypanosoma brucei brucei LEVO MQRNLTKLSAAAFYEFVDNNFLNNKRPPVPGGSWTVEVLRNKSLADLQHIWFLLLKERNMLKSMKEHYLRHQEELGAMPA PSRLKMIDESMRNIKRVVKERDEEATARAVEIFKERLKRGIYRYPPGPPPPPGAHDKTSVVKVELSCYVEEERLRELFGR YDVFEPHKGIVRVELKLPDEVLKQKEEAEQLWTQYMAECSDVKAYHQWSTAAPSAYDYTEVELAPGIFANDAISDKGGKH SGDTETHEGVIVAARVPVPPPKEKQPPPKNPLERLKAERRSYLARTTIQLGYFPNVTLPPPRYETVEAVPRPVHPDEIEG PWEAYITYDREDGLSYAQSLGITTIGVATVLGLTEHVREPQPYAVVDPVYCEALRRERAREETLMKWPHVPEWKYEYSTY TRKHLADIVQYNYTNVVDYVDREVLLTGKSVWECPIHIDHTCGGSKTVPPHAKKPVRYMDAGIANVGVTDI Q38EM7 ul30m Trypanosoma brucei brucei Lister 427 0.025204334 1 Trypanosoma brucei brucei LEVO MMRRMWVPTFSAQQQHLHHAMVLKMSTYASTPSGNGVTSAASNEEMNLHDAAAIAPGPYRRVGNIFIVHCDDHPFKHSWE VNRMLRELRLEFKGQTTIVPDIPQVRKRIWRVRHIVKVDVLDLDEAKALIGVPEHISFTDLASQLPPSFGRVKAVPSPVI RSKMNFMKLRRMRLRDVLHRDALELRLLELKRSAMKNAEQQKHAQKDERTDNKEGEKC C9ZY77 bl31m Trypanosoma brucei brucei Lister 427 0.021083229 1 Trypanosoma brucei brucei LEVO MVLHKWAVVSRSAPPPRGLRPIARTIPTHPRLRPVDYKIPYVLRTFIKDRHTSEVQHLENRGMFAEELSIERSRFPRFHS TFTIQTDGSLNEREFEFAVPPIVTLFHDRLSAHRERQLELAKIGKLRKERNWETEQKGEESVSMACNALAFPYCIPKNML KRSRVVDPLNSKSSTQGVTSGGG Q38AB0 bl32m Trypanosoma brucei brucei Lister 427 0.00974571 1 Trypanosoma brucei brucei LEVO MFRRTFFTPMIAQPTLLMLGNKGGTPKRKKNPMQLRRKVYGLHFKEKYLKMEEWYYCPLCAEPKKPGEWCRREDCRQIKP Q584F4 bl33m Trypanosoma brucei brucei Lister 427 0.013042739999999999 1 Trypanosoma brucei brucei LEVO MLSSTALLLRGQYKTRLKKRMVGFIPKVIPRKIKNNMVALRSEANTGHMEGYLKTETERLDATGRKVQKVLWDPVLQRHC LFKETKIKGPFMTKSAIAKKVDFPIGGAEKMLKK Q581Q0 bl35m Trypanosoma brucei brucei Lister 427 0.021661453 1 Trypanosoma brucei brucei LEVO MGSEESNNICAYKRTISLAKIYIVLLVKTAMLRYSRLCFPKMGCEEITRKARRVQLQPTEYLAQHRMQVWQLRFKEMGPP FSRVWVALGGKMRRRRVGRQVDVKDMRYYWRPIEPQYQRLYMSRLRIRDHSNKLRQPMRLRATNADIGSGSSSIEWERAS NRKYGAMLAPPKRQDFEFRVV D0A1K1 bl36m Trypanosoma brucei brucei Lister 427 0.021795226999999997 1 Trypanosoma brucei brucei LEVO MRGTTKPMLFLCSTAVRLNFFPLGLSAGPLNSHILLPRNNFDGRTTHGMKKIQGQSKDPQMVMSRDELKLRCEYCRFEWI HDTLCVRCPAQPSHDQREMWLHSTWMWGKQQPYKYYKYMPAVRNPRTGMPMAREDARSMNNERRGQGLTTRTLNLEKERR GISRDVSRIGQYNTRWKTRFPFPT C9ZMA9 Ribosomal protein L3 mitochondrial, putative Trypanosoma brucei brucei Lister 427 0.055073699 1 Trypanosoma brucei brucei LEVO MRSLIAMRRRFVSGTWTIAVSTQQRHRSGSGPGDKRIRTDWYRCYPSLMREKDRDMYHCYYPYLFDHGDKMSLYPKIPEN PREWQPEQLQTTYDAIREDKYDAFIRLREKFPELYQDTRAWDNPPPFGEFNMFYSVRFGMVGVKAFTCKDYDELGNQFDC TAFWFPDNQVVKHSTRNGEVGTDKVYVGAMNVPVEFHKPHVAAFYKAAGVPVKHVCAGFPITPDAYAPVGTKLDVRHFKP GQEVTITFQNTDYGFRGVMFRHGFDGGYVWLGDSRWQRRPGAMGTEGQKRIYPGHRMAGQTGAAAETYQGVPVWRIDYKN SLIYLPTLLDADVGTYVRFSDTINTKGLTLWNEHRGLPAFPTFIPPEDEDLSKLATDECQLKSPPLYMYFRDEFPATQLV SQADVEDAKSAKPATAPPKKKVYDMKKYYEARKKYRQSMQKARKYKLMGLRTKAHEKQEEARRAKILKYKRMK Q580R4 Ribosomal protein L4/L1 family, putative Trypanosoma brucei brucei Lister 427 0.05315666399999999 1 Trypanosoma brucei brucei LEVO MRRVSFSVVLATFGKRHSSIFSPRYDWRTSGVHDIAPRDEGDFLYQGPQHVLPGAHPLPLHHPHNTITRPVISPYIPSPQ RSHPYFTAPLPELPHFSTTKPIVYTYGTMKERIIAPVFNLKNEVIYTRELDPFIFGMYPEVEELSKNLTYWMVRCQNFAS KWDYETREIWRKAKKNWPNTGMGMPRVGNRKNHLYTWGGRTKPSKPWNMLMPTMDVKTWSKSNRMMLTLKMLQGRLQVVD RLTLEEPTQECYLELCRNMSWDVRHTGGGVLFMDGGSRITPSSEFDRAFFFGSFFNGRNKIVRPTVLCDEQYDYNKTAAK QRMKGPKGAKNPIPINRFNAYDAMKHDRLVITEGALMQLEDELYEHKLQILPPHIRNQLPEYGYLDSEALGDCVPSLKTI QMEAAARTEEAESDMYKSFIDNPYNPWKDNMDASYAVDGADGTVQKFVDGKKVSWSMLS A0A1G4HYD1 bl9m Trypanosoma brucei brucei Lister 427 0.031032696999999998 1 Trypanosoma brucei brucei LEVO MRRTVVQRVAPWVPPPRHDIKVTMPPPPGGEVGGRFGVSQGYSDRLARTPYWKRMALSTYKLRMMENATRYPMSEHRPGE YDIRYLPTPYPCTIRNRPLLEVGEPRQIPSIRIPVIFLVNLFDEAKGCWFGRRYETVYVERQFMREELMPQRYAIYATPE AYKLLGLPVVNHHTHEEIPKTPREYEKLLERQRYDEERWKYTIEYLFRKYEDGPPELLDRPEDGWDGSEEIALSSVAGAG RDGVVSQRKGPVKQRKARKIKLF Q57UC5 ul10m Trypanosoma brucei brucei Lister 427 0.020283566

residues 106-129 built as polyalanines

1 Trypanosoma brucei brucei LEVO MSPASPLPVAALSRLRITHRSFLTRSRGRHVCRSAVGVEYRPEQQKKVLDHSYARVINAEVVHGDEQKFWGERRTFYTQR NIFFPMWDRCAQALILITREVPRVPQEMAFRLMAVFLKLMLLPRLMMNTELMLPMWIASNAEGAMAAAKDGSKGKEQSSK QQGESKDDAKKEGDNTK C9ZPD1 Ribosomal protein L11, putative Trypanosoma brucei brucei Lister 427 0.04025275 1 Trypanosoma brucei brucei LEVO MLRACGVLQLRARPKTVCVEPGSNRLPEALVVEKARDIFGRPEFPGKRVLHNWRFFIKAGKAATGPPVGQEFSKLGLKAM DFAKVFNDRTKPHFKEDVELIVRIQVYFDKSYLFTIEPPPTAWFILRALRKKRRETGPVPLRGHYCALMTLEMAYEIAKM KPLCWGRPEYPLLETRVRRVVGQARRMGVCFIGVDTPYSSPVKDMTEQQYTEECERYRRIHMEQYTTLRQRELEEAPLIE RLHRPNMSPLTDEQIEEGLRDPCLLDTLWRASHPLSPYHRDLRERELARRYLNARGWVKDMTPEEMRIVFMNYRLPEGEK RKQMDEAAMSGEVYWTRDGAQL Q586R9 50S ribosomal protein L13, putative Trypanosoma brucei brucei Lister 427 0.024341309 1 Trypanosoma brucei brucei LEVO MQRSTRIVMAPFKSVLRRHKHHPEGLPLYNDMSKQWLCREGERWWLLDARGEQLPRVAAVAAQYITGQHRPDFTPGMITG DHVVIVNIKDVVMVGDQWIRVPITWQTAYPGGKYRIRLTDMYERDPCMLMWWFLKDEVNRHFVRKLKTRIAPLEKAWLYE DSIHPHADKNPRPLCWTDPEVKGWKYRDPQFIRRWKPNEFMH Q580D5 ul15m Trypanosoma brucei brucei Lister 427 0.043780016 1 Trypanosoma brucei brucei LEVO MLSVSPPLRRYRLFHPRREAIPMHMCPAKTIFPLINSNNLLVKTRNSWEDFTGRKEFDEDHPLPVVGSRLNGRTTQHKWN HWDQYLNPQITQSIKDLTPTPEYVGMRCGHNMIKMGWMKIGGSWKYSRGYNDRRRVFARGQWQERKMTPRFMLAPRVSAG GPRNRYEGKLVFSPLRLSKLLWAIDTGRINPNEVITLYHLRQANVVGEREIVWPGFVLISNGVRRVPYPIHIELQNASAE SIRLIEEAGGSFTCVYMTHEGLYQELHPEEYPIFMDQELPERRGLESLATNPSKRGWLTRWYEDSSKYAHPAAGRRYSHY LKPPTERDFPATVEEYEMVKHHQKWHLNQPGTGTLLPWHSYNTADLVKRAAGRL Q57U68 50S ribosomal protein L16, putative Trypanosoma brucei brucei Lister 427 0.019158144999999998 1 Trypanosoma brucei brucei LEVO MPSPFQQTLLARAYHYVHPKTGRPIRQFRRYGDPRYINNEAALIRGQWGLITADFGMVTQSQMENARLAILRRLPRGSFT LTMHTDYEEFPVVKKSPESRMGAGKANIHHFAFKFTTGVPLFEIMPISARRLNQAEAEGIFLAGRPFIPLQTVVVPQGRV DEYHVFK Q57UM6 50S ribosomal protein L17, putative Trypanosoma brucei brucei Lister 427 0.035117824 1 Trypanosoma brucei brucei LEVO MLRITLLVVGRAPPVIGCTRKARYAGIDDNPTITYKPWDTTEPLMADYGWTRGKLPKFRARSPFHRQQIARRMVTEMIRK DYCIVGGARAPALRILADHVVELAKAGDTDSRQQLAYFLHDPLMVDKAFDEYPRRFKDMNAKYAMMTRLKSRRRTDAVAM YFVEYKNRDMSDNHKGEDYSAGPERFFLPPRIVETEKGIQRPPHMQMAFDRWASKFKTEEFHHWWRLRHAKLRYWGVRNV PHPSDVDPLWTEKEEEEWHNEMLANTGEYEDLDLDDESYTAAGEGGEPSPAGDGPQPQRVG Q57YI7 bl19m Trypanosoma brucei brucei Lister 427 0.016931455 1 Trypanosoma brucei brucei LEVO MGYTRERTNRHFFVARANAFFSRLPIARVQRSLAMEAVREGRMRPWKYTKEQILGAPVTCNFEYNPRPVRLIGTVMDAHT EETSIKGGLKVYARNEETNMMLWIPPGNPKLRHEVTSTKGSFQHYLDERDKWDEAWITGRARMK Q4GZ98 bl20m Trypanosoma brucei brucei Lister 427 0.024393942999999998 1 Trypanosoma brucei brucei LEVO MLRRTVCVQHYRAKLELDRIRSMLRGRARLERKVGLKRLFFLMRTQTRYRVEQQAHWERAIVRKNVDSAAREHGTGWQHL RNELGRQNVMLLPRSQQLLAQYEPLAFRAVVELCASRIPPPPPPVVASVPEESYTLWPPASHDNSECASTDGSDAPHGQQ QSLSHPAARVELRCGVERVLRRGPSGLGNNVNELIDAWKEFDVSPLRKGEVNK Q383R2 bl21m Trypanosoma brucei brucei Lister 427 0.020895267999999998 1 Trypanosoma brucei brucei LEVO MPSLANGLRCPDALITRTLNPLLNGPKFAVIYCGNHQYKVAVGDVIAVQRLRVEIGSHIALKKVLMVGGPRFTAIGRPLL EGVRVTAQVEEQKRMRNVVSLFATPGRRHVRWVDAPHAATIIRICEIFYSPNVVGELDKYKGELLETFTPGQHTNPVYPV DDGYDVFRKKDKEAMENATTFLDLLGGV C9ZT11 ul22m Trypanosoma brucei brucei Lister 427 0.032083284999999996 1 Trypanosoma brucei brucei LEVO MPRPAPRFAMGHMLPHRSNVGSQFLHTQRHGSRSTWYKKHYFSLRPFAIQRHHGTTPRILLDRSLWKSLWITKLQLPDIN RWERVVNSRRVTEDRYAFVEEEGVMHKVNWGLYCERLETELTVTQERLPQHTLLMKAVPSSWKKLDIDISVIRGLSLREA MAQCKLSLRKGHQIVFRALEMAQQGAEAKGLDKEHLRVAHISCYPGPTDKQIDIRSKGYYAWKTKKSSHLVLTLAEDPEM VLPDRTCLPYSSLMSMKRAGLSAQPTVIDVPAITADGI C9ZSI8 ul23m Trypanosoma brucei brucei Lister 427 0.028822936 1 Trypanosoma brucei brucei LEVO MVVSLRRFHSYANSSRGGATTCLITGGGKGHFALTVSTRGRFYRPLVDDGINLWRRRMGRIHKGWRTWEYQHTRPDPRPF PDPPVNDYFGRSRIWNPIACKLGYVRKKADEWGWPNKPPPPTGLRHSQEFFPFFFERYFPDAEVRLLLDSVLNNETKRPV FQIPCSMSKVELVNYLKNIYGIDNVVRVEVRNRRGRRYKNEVGEIKMMDDYKVAVVELDTPVSVELKQIKGTEDTSDNRP QERITQ Q387G3 ul24m Trypanosoma brucei brucei Lister 427 0.043999047

residues 313-340 built as polyalanines

1 Trypanosoma brucei brucei LEVO MYARFRTRSKFYFRPARPALAYNVDPNVMRRPKVKRGLLKGTYSDETVDLRDRERLELLESMRHPRERDFYQDHTYHNQW LRRDLEKHQKQQLAARYKYFAPDFEISPWIWYPGDIVEVVSGEGIGQRGTIIAVIKYKNEIVVQNINVQDVVIPASESRP EQIVQREHPISVTRVRHVDPSTNEICNIEMVKVRNKETGEMEEKRMSLESGILMSIPPVNDELEVGDPLKDTPIQDADEA TYDREAEQAVLVDKRLEAMEEHFVQSLKQSYEFHEPLRRKNAEDMRQFQTDVIDMACAMLGERLLDTVNASDTSSFPAEW QEAIAMHVEEIEAEMEEVAAKEMAEAKEGGAAENEQHDDDLDDDEDELDTSEESVKHV C9ZK52 ml38 Trypanosoma brucei brucei Lister 427 0.058437895

residues 41-53 and 294-311 built as polyalanines

1 Trypanosoma brucei brucei LEVO MVMRRCSYNVHPTWYMMPLRAYHMPGWKTKVSTKDVRTREQREADRKVRQIEKLPRPQALHDFQYPYEKTVLSDTMFQYP VYENELDTPHLFNITEPPDFFVYWNVSDFERTSYPPLPEEDHRLLLPLSGSAPWKQHRKRSLKYLRKHEILPNYLPHVRQ DVNLSVVFPGVYATRARLCEETGEPLPPPPPVSRMTHRNFWMTAHCGNYIELADLQHPPSIFFLDTVSATGQGKAVEKDA TEEGSDEVWYTLIIASPDYPFRVPASVDASTQRGFFLNYMMSNLKGGGNSTVLEEYESEQRQNNQKRQHLEELVRNPAPI AKEGDVIVSYTPPLPTEDAGTTRHICMLFKQRSYVSGASCALDDSKASFAARANFRLHAQHRDGIPSSSVEMLSRIEQVL PPDPSAVTFFQTKWDIQVQEFYESRGMLEPAAPLDEEIEAILAYHARKPSELRVRARHRPDGSTNVGDDPNFWAQAEPTR MMDGSMLSLWSRRTTLGANGVPITYRR Q381T7 ml40 Trypanosoma brucei brucei Lister 427 0.033646065999999995 1 Trypanosoma brucei brucei LEVO MVLPRKLVGQCCLLLQKKQTAASYMANAGKVGNEEKWAQAAMEYLHEKRHCNDSRKRQHDVDNERRMAFAFDRYCSVNEK IFTERLSRLSDRMTEALETIKQLGMDHALEEALMLSSEQPPLNFRRPTLTPPVAGYEPGFGLDVPQLRSRQAEYPPVGRP TDAMEFGEEKDPSFPLVESFRVEDLTTQCLNELEERHGEIREAAPTTGVEGEAWEAYVALQKKALARQQLIFELCNNGEL RERYDSDVAFRQRVWEERGMLPLEIERERLHEEPRHYAQEPAYHPFRKM D0A511 ml41 Trypanosoma brucei brucei Lister 427 0.022556877 1 Trypanosoma brucei brucei LEVO MLRCSCACRRGVYHNAPSVYPFVKPFHDTPYDQDRGRHDSVGQRYRKNSWPEWMDNGADGTGYGIGLHRTHPLSRLRGNL KRSPSHVPRVLGMMIQGVWHKSGVKLYFRGGKPPNPSVHPYLTGEPCPLYGWKVTDESVIRQFNMPSIDGTNFRYKPYVA LQERKIMGVQVPSDSVSLASGRTTESKPLAKRLFFWR D0A8I6 ml42 Trypanosoma brucei brucei Lister 427 0.021602639 1 Trypanosoma brucei brucei LEVO MLRLCRVSLRVQSHQKKRAQHPNAGTRFGRVYNRGFIRYGFGGFGMSVYTPKKDRRFRVQPLPSLHANSLADDTPLVTTT RTLSPNFRAFALQDGGVFFTHPSHEQVMRVGQNILAEETKATGMTSMDTYVNSRIQSIIAENTVENVALSHWRRRHMWNL VRTHGKLQRHWGVSDATKGARSNIYGRPS Q383S1 ml43 Trypanosoma brucei brucei Lister 427 0.031300405999999996 1 Trypanosoma brucei brucei LEVO MSRNGELCLKKVIISYCPSNGAPNTRQFLATHLPHFHAKYPSVSIDLRPRLWPEMAITGVYRDGSERSYNTKNLSPMGIF LRLNNLVSTANDYDQPFCASHLHFQRRSVQGTWNPWLWNYETERRRTEAPQWRRKLSEKEWDYYVGQYSAQMKQEEDEIQ RRVADRTCVQEQSTREVQERWKRHVVPRMQTDLEFNLSHFKRQHARGQLQQRPVTMGEYRLFSVPDPRELGQDAVDTMRR RESHNMEVWWRKRKEQLKPP Q583E5 ml46 Trypanosoma brucei brucei Lister 427 0.033984199

residues 149-159 built as polyalanines

1 Trypanosoma brucei brucei LEVO MRPVARLPLSRVLSGCRGFHSQHRTPVLPQVPAAEVQQAASESKVRVAYLLHRQPVVKPTPHPLEMEMAFLLQREHQRYS RHESSESATHFMAQRGQSIDALNRTDPRQIQSNFFGLELYQDAMRVVLQRYKPERRVTPRDLWDPATYGSSNANSNASSP PTRHSLHRKLDDYLHLIVRDEASGKWTVPQTELRGRETLRMAAERAIATDNGEGLDCYVWSNAPQATVPNANDGSWLFIY VATYLSGRPKFSEFRPKTIDHAWVTRHEMMQYEENFQSPELVRVLLDISADSTFES C9ZT91 ml49 Trypanosoma brucei brucei Lister 427 0.024501486

residues 102-109 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLNRSRLRRVWLGGTAANTSLGRDVMPSTGKYINIVSPTDVAANISAGAMPPEANVAAALPVPRIQYSKWNRALRSDVYD ELLKLPLRYKLHDFAKLCPPQRCQEEPEAVGVVGRDSAVGYLPPLGPADPLDTIPFFVHRSSNGVLPGKVFSMHPRNLMP AFYLRIQQVEGDMFRFEEELLKIFPTKKIFVRSHSIYVYNVALDGRMVLHHWLLGLGF Q57Z82 ml52 Trypanosoma brucei brucei Lister 427 0.029175984 1 Trypanosoma brucei brucei LEVO MRLHTVRAPIITRAAMRGYSEARSNYDGTSLPAWPAPGKKPTYPAALSELRLPQPRMRKTRTEWMYYHGHGGCPGKYGPS REIADFEYADGTPASISGRRFAFKHHQDHLLVQLIRAAATVERYDASGLLPRIPGTAEQRNWDPAIPLFLDDVDEQGRPA PLRTAGDAPGTMVSHVCSRVVDERMGTPTHTPNELANRHEGETLEANTMFATNDPSAFVSDTVKLRDDKRPYWSRRRWAL TDKFLVPKSPKPKNTIKDE Q385V2 ml53 Trypanosoma brucei brucei Lister 427 0.035656383 1 Trypanosoma brucei brucei LEVO MLNPPKHYSVESLRTVGLLPAQLALSRKPRLRPHVGNLKGLVYPLPYYAMWRGNHNKYTYNKSTVCLWGEGDTRSMYHQH YAHAKCPTDYGRGGREFEYLTVKRGKMLQKPLPRVQYVAEGSKPVWLFKSWHTPLSSPSMWEREVQYAEHTPEHIGAKRP LAVVAPRTMHRYLFLMHMEKVTITVSPLLFGYGHTIQKAVLDFYRRAISARSPFPKDKVFLFYAIDHITPRIEVTWLDGT SYVPPVLEGASSQDLIQMVMEEAWLAADRMAAEGRVLNPLAIDDYKWDQLVVFKKVRDKEASKGGGRKK Q57YA9 ml63 Trypanosoma brucei brucei Lister 427 0.018213924

residues 64-73 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLRHCTAHRRYRTAWRELLHPLPVRARKMEWLKRDAVEENEEILRRPYYTIKSYALPPAVGRQESIHNSNNIRGGMHSSH SLDLIMRQPRRVKTPEQLRALRDRLRFIGVTGPMPQATSVSTKSYTDTYGSRLRPRYPESWDTVPPHQPSRELL C9ZU82 ml64 Trypanosoma brucei brucei Lister 427 0.028545976999999997 1 Trypanosoma brucei brucei LEVO MLRGTRGFLAVSPGVGIAPETTPVKYTPMMLNIQNMMWWNGKRNLYRATYREKTWYEISRTGAFTKGRRPVMRQKYSREA LQAALAMVPPGFEVADVPRPPQRILAQSEGIVGRWYSNYWTLHSMRYQCLLAGVEWPLGERQRPRTNYDEPFFFADFEES KAIRDYRSRWINVNRSLVGMTKRMKEAEEEARYMQFRKLQDTFWSNRKVLVNRVKSMYNQGARTSAKDMPIKTINIKAFL SE Q383B7 bL12m Trypanosoma brucei brucei Lister 427 0.003998369999999999

residues built as UNK

1 Trypanosoma brucei brucei LEVO AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA bL12m Trypanosoma brucei brucei Lister 427 0.002008194

residues built as UNK

2 Trypanosoma brucei brucei LEVO AAAAAAAAAAAAAAAAAAAAAAAAAAAA bL12m Trypanosoma brucei brucei Lister 427 0.0019371159999999998

residues built as UNK

1 Trypanosoma brucei brucei LEVO AAAAAAAAAAAAAAAAAAAAAAAAAAA 12S rRNA Trypanosoma brucei brucei Lister 427 0.3767485 1 AUUUUACCAAUUAAGAAGAAUAUUAUAAUAAUGGGUGUCUUAUAUUUUAAAUAAAUAUUUAAAUUCCGUGUAGUAAAUUU AUUAUUUGUAUUAUUUAUAUAAUAGGUGUAUUAUAUUUAAAUUUUAAAUUUGUUGUUUUAUAUUUAGAUACAUAUUUAUA GAUUAAUAUAUUUAAAUAAUAUUUUAAAAUUUAUUGAACUGUAAUUAUUAGUUUAAUAUUUUUAGUUUGAUGUUGAAAUA UUUAAUUAAAGAUGUUACAGUUGUUCUAUAUGUACCAAAUAAAUAUAGUAAGAUUAUUUUAGUUGAAUUAAUAAAUAAAU AUUUAUUUUUCUUUGUAAAUAUUAUGAACAAUUUAAAAAUUAAUCUGUUUAACUAAAAUGUUAUAUAUAAUAAUCUAAGU UAAUUUGAAUAUUAAAAGUACAAGUAUAAUUUGUAAUUCUAAAGUAUAUUAAUGGUAUAUUUUUAGUAGGUAAAUGAAAA GUAUAAAUGGAUAUAACUUAAUAUUUAAUAUUUGUUUAAUGAAAAGUAUUUUAUUAUUAUAUUGUAUAGUAUUAUUAUAG UGUAUAGUUUUUUAAAAAUAUAAAAAUAUUGUUAAUAAAAUUAUCGUAUUUUAAGUGCGUUAAUUAAAUGCGUUUAUCUA AGAUAAUUAUUUAAGAUUAUUCUUGUAAAUAUAUUUAAAUAUUAAUAAUUCUUAAAAUAAAGAAACAUCCUCAAUUGCAA UAUUAUUGUAGCAUAGUAAUUUCUUAACUAAGUAUUUAAUUUUUCCAUAGAAAAUUUUUAAAUUACAAGAAAGAAAAUAA AGUAUGAAUUAAUAUCAAAAUUUUAAUAAAAAUUAAAAAAUUAAAAUAGGGCAAGUCCUACUCUCCUUUACAAAAGAAAC AUUAUGAUAUGUAAUUGUAUGUUUGAUUGGGGCAAUACUAUAUUUAUUUAUAUAGCAUAAGAACUAUAUUCUUUGAAAUU AUAAAAGGUUCGAGCAGGUUAACAAGCAUUAAAAAUAAAUGUGUUUCAUCGUCUACUUAUUACCAUGAUUGAUUGUUCAU CAAAAUAGUAAUUCGUUAGUUGGGUUAAAAUCGUUGUAAAGCAGAUUUGUUUAUAUAUUUAAUUUUUAUAAUUAAUAAUA AUUAAUAUAAGUACGCAAGGAUUGAUUAUUGAAAAAAGAAAGAAGAAUAUAAUUUAUA M94286.1 ml67 Trypanosoma brucei brucei Lister 427 0.093569273 1 Trypanosoma brucei brucei LEVO MLRRFALTSSVALRLRFERDSGHNTVRYRPIPESMQPKHLEDNFTPFPLPKFDESLEYGPVRLRNIPDIEAAKERRRGSR LAATEVLLQETLQEENQFATSGKGDGNMAIAITERHTEDVTTPAADSRFPSQTMSPCSHEEEMRGYVVSRDYPLIDRLHC TRSIEELVAQFEDRPQIESRVAALADMASTVSFRSDEELLRMFTAISAPFSVDGRGLNFLTVKVSKFGRPYYVPNSLLPA YVNLVDATTIALVREQPWRLSASPALFIQVLQFMALIKVFEPNKWFTFSDHAPSNRADYRHAIGVNHSTAFWGTGEELYD FMVELLRVEDDGRIPTMLDLCTREQMVDLLSGFCGVMPCGKAVGDVFKTITDAFLRRVRNDISGPWSAHDWAIVERMYLV TVLCDAGNNEILQLLLSDTASPRGPDFFAAVSRTKDTPTKKRALCLLQEAIDNASAKADKVTLLGLLESGSEFLLSLVDK GVAHTFATQNLFDYRILNSFLHCSLVADRLRVEQSVITSLIPSSLRDVQVQMLMSNERNALNPLTSSLPGNSGAIATAPK LKRPLMTMLSQLEYLNSIDSVFILHSSLMATSTDQLVSAVRRLPSGKDSLIVTMSCLRALSVKSLTSPSMKERIACARAL EIVSYELEKGRAVLLPFSEEILLHDAGAYCDEDLMLWTVAAFLARELPLVKVHTLMHSNCTARTPYRFLKGGHNLLVSSR SLYDKGAPLLSSLHSKELRLVTHNVRLRTPVRDRKCTLQYYNPIRARFVYRRDKPLFDKYHVTARNLAPGFSRGALKHDW RALGVYTPDHPQVPYHPLQTWMLGETTVEAE D0A5V6 ml68 Trypanosoma brucei brucei Lister 427 0.062806293

residues 264-343 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLYTRRLMTTGGSATADGAVSYSKGSYHIVPKKYTVGKRIAVRSYLDRNRTELSDRTYMPQKAWFEPYTPKKFDMEHQRI SHNFYNLETKLIWTAFDTPELIGILLHDETIKGAPHLYDAEFLESAVHWTRESRYWRCIGITKPFYNKTTLRAQCWHDRG LQVGTLVFSQAMRDALMDLERAVRRKELGLEPNYVWDRWGPVGFIDGARTDHLPRFAHNPYVDPDGVEVTEVDIAPFNTH EQIKERYGAFIDPDLRPFEGVFRAPSHGALTLDDVPHQEAVRLYRDLMEKADMPVMLGNGAEIPPMDMRALFHLSANPER MKAASELSSWREVRGMLAPVQEVCDEKVEALRLMENTRHDAARVRTFYEEKCGFSDFMRTPDKVITAAVLCYLQELQRIC TETDWGKPLARCLTDLERVNVMGKDAFLVYRHIEDAILDKKRRVWATRFAGEANEESTLDYLLENFGRRTEQTRNVGTTG TEFDREQEPIGRQVQRRVLDSDKASKLAEVRQKRGKMWSNKKSVFDSLHQKQLQNVTYGVH D0A135 ml69 Trypanosoma brucei brucei Lister 427 0.059010855 1 Trypanosoma brucei brucei LEVO MLRRLAGCVAFRKNVVRPFMNSTALLSSPPAAVSAPVIPKSGNEGKDYSVASGSVDTPVLDQLVGYYVNIQSRKMYYKDF LRIEHQAVRWAFADIRKNSEALAHGLLQTGLRPGQRVLAIQPCNCETFVLQLACAKIGALLAVVPHHNISADKLRFYLNE FQPHHLIAREWIVVPEVKQGVLVERNMHFWDMIYNVIPELGLSYPGQRFQWVHSQEFFFLKKVVITDHNMNLLGVTPMRK MLVWGPFSYYEQRLRRLSALLHPDDPILALEDPHPALEDKLHITYSHRNCINAGFLFGNLMGLKAESRFGVMPNHHVDPV GAIVAPYAALTTGAVLVHIHSDMFTDDHAINGIEKLCVEEVVGMLGKKSDFDLLLRHAGNFDADQYEHLKWVVLFEDASE PTVGDEYLQKLAKELGLEDVFVFRGPLESAYMVMWRSLKRARWEMVPHVEAKVVGDRGTADARILAANTRGNLKLKGPHI SAGYYNNAGLLTELVDERGFCSTSREAVMDERGQLTLHGTQLY Q584V5 mL70 Trypanosoma brucei brucei Lister 427 0.062442410000000004

residues built as polyalanines

1 Trypanosoma brucei brucei LEVO MCLKRKAPHLFCFCLWSIFLSFRCFCFRSYAIMLPLLSFPTIQISIFLSFKLPITTFLLSPCFVFVFVFAIRYCGELTLN AQLVLFLLYHCAQTQRGPLKEGEMPICPGLCGELAAVPFRVFLGTLPTLAVEERFLRQLQPVFAWYSSRKRVKEQANEFI EIDLASCDAELLLRYSHIYYVRRQLFDELIERQMTLLDSGKAPKMAEPSLLQCLAGCNMTIADRLQLEIRQLGAAKRAAS VPGRRELDPVARLEVYDYACMMRLVEEDAGAVGDAEMKARAYLPREVIESKLGHLTQLLLGSDARAALDKKDVKLLNRMI PPDYTRVGCVEKLRPFDVTAYFRFYGERINNVKVENYFKRALWGHVYRRFATTPSFLSGVSTYWARHSGLDASFTTTTMP QEVAVAVCDQQIQFPAIKFRAQYVYTSPETARQLWRTDAAVPLMRLFPLMGSRTAEDLAAGVLTDAFWMHLGLSEEENLL QDSLLLKVRRFVDEVGDMYETNIDSVLKRVDDNFKQVVPQLKAEDLQVDAPLQDGEGEDTVRETVAA ml71 Trypanosoma brucei brucei Lister 427 0.05031379699999999 1 Trypanosoma brucei brucei LEVO MLFRSVSCKNYQRGGWSPGSKHQKHMTLNPTLYLYRFPGPHGPGPYTMKYWWTLGCFPTGMEVPFRLHEFLSTYQQEHVP VEVEEWLRCYIKDPLSELVNASNDFFKAVEVYPEVESARGYKTLQPSIAPLLVPMKKFEEQLGVKISPVGLRSVLSNPVL KDRFLDDLFDYKSYVEKGGSTPHRRLARSRFEGSLSVLGECEKCLPEQHQVEISESLGTFIGATVSPAETTADDERSLIL LLTTISEGCINAGNYSDAASVLADALMFCHDPDSQATTHANISFASLLNADFKGAEYNGREAALLQPQVKPTSTACARGY VGWAAAAAYQDDFEKAEAIVKDGLTLYVGNEHLEKLANKLQALREEQPSVYKQVPRSLRESRSHLPSQQSRGLLSGSGKG FSNEFDWVEFKNKLYPSKMDPRNNEMGSVFRRVGDLGSFISTSRSMERL Q57WG1 ml72 Trypanosoma brucei brucei Lister 427 0.049407 1 Trypanosoma brucei brucei LEVO MSRSAFSSRGALLFSSAPLQCQRRWVTRLYTSYYTGVLYPNQLVQPKQRLPADVSVSAILQKRSEPEAAAERGSNKGDKV DVSKGEKTPRVSWDDADSTDVMRVDNTQQLKEAPQVERPYVPLGEVAKLELQGDYYMEGGMFQEALEHYGVVAKAYNYAY PENHAQRIGIRIKLSAAFRQTGRLESSLANIEEVLRMLDASTRPSLELICEALLELGITREALGMKREATEAYEEALEVV NSFHNWGESHRMLRLLPRLGRRFNYNFEEKFVYFSPFDYDRTFALVDQCLERAETIFNEIGDVEGAIRVLQQRKEMIDKK FFNMRDFAGRIHTMRGHWKRRAQHLTNAPTPDELLRYSPTIHQVHRDFKYELTAPIGREKEVMPGVNRLVLDMGNPYRRR GRLSNKMLKDADHKFANYVRQKEYNE C9ZR63 ml73 Trypanosoma brucei brucei Lister 427 0.041857874999999996 1 Trypanosoma brucei brucei LEVO MRLARTLHHVASATTGGGQMLEGLVNDGPEVAHKQHASFTPFSIQPWQARCVGASRRKLLPQMLMYHGARLGPRPLIILD HSTKGEAGVAEAARKYESILSQLSWDYGAVYIPLHAQCTDSSKDLLEQSCQRICAVMDALDVRWTHFLTYSYGALVAVRM ASSQEFPHRVGTLMSLDTPLVTREFLRNMEQREDIAKAERDINVPEDGLAFAKQALLSSLEGPLPCPAAEDESLYRDYLF DPNRIFGAGGLVRDESRYVPLKSLLGVRHPVQLIVPSANPLSDAAAHSEVFGHRRPAVVKCCQRHEDLFKESAAKEVAGV LGAWMRRFEPDCFISKRYEQAANEMGQLMLSTAQVSSESAGKGGGEPRKKKEKKKSKA Q57Y49 ml74 Trypanosoma brucei brucei Lister 427 0.039765176 1 Trypanosoma brucei brucei LEVO MLVPGLSLTRRAVTSSCCRPLHVVRGFSTTCTLFGLEQLQDVPTSTSRRPTGLHRGPGKRQTSEREAAQYKFIRRWELQM RDEWDQLEPFKGLPKPKRQFGNEAAEVIWPYALLLERVVKVHPFTKSIYVYYAQRQSTARGKLAAEIARSFAREFLIPIT FHNSQVYTEAEMLLEYSETPWVVLHSLDNGQKPRILPVAPVEGTPAHTAVEQLLAEVVQGCEALGASVADPVTATRVLNE RPLQNQYVRVDYQWFGDTPDERASHLVRWEFEPEQIEPKIRHRTRHVLDWLNYDGNLPTHRAVHVNAMREKARQKAPRTV AGPRTFYNSAGSRANARSSRFGGQAAVGK Q38AM5 ml75 Trypanosoma brucei brucei Lister 427 0.039841616999999996 1 Trypanosoma brucei brucei LEVO MLRRSDLLLKKGWTHNPGRTRRGGKNLAWRPKMSERTLEQFVPLHLAFPRRHPNSWQERQFHLLGYVKWPKEIGFYNAGD NFELTPQAAYRIYKQNCDETFWTRLHNEKTIIHLLPLVEQDPGTNMVLVDDIFRHHLKRFGADHYIYNAVMQAAAFAKDF PRCEQLLAEMRGLGLEPNAQSYVNMMLGARLTGKPRDQAEAFFREGIKTGAISAVMRLDTEFQMWMNQLERLGSFKAKVG YLSVNEEGASPMPRDMWALWGWHRTEAKFISRKQMISEQVQNRVRSGKELVGTVYQKARRQPWAKYNGMFPYDYNGPARR PAASFVDAPTPTHNAEVCGTAYA Q38CK0 ml76 Trypanosoma brucei brucei Lister 427 0.039638914

residues 168-176 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLRLSSWNLKSQHHNVLRRSRPHIHKYRELNRWQRQAQGISKWDQSHSHRPLPYVERFNPESVGLTRGTSAFAWKWWHTQ YPWLPNVPPEAAQIDEAQKQERRSHRPPAWDDEFAKVVLNMNDAEIREYLMSKLTDVIFLETQRDGYELRRLDFEGKPLT SLPEPRIIENFVLEEETIRERVIYQVVEGVFRLSPTSADRRELRSVANIIDYVLTHVRAARPTDRERRQERPITSAALAV MQKCPIQPQLGFVHALPHDTRDALLQEWERMHHLDWQFGKAVYTPRSKENVRGNLTWLREDRHYDQRMKFMQEVESGEAR AKHMKLIAEAAGN Q383M2 Chaperone protein DNAj, putative Trypanosoma brucei brucei Lister 427 0.043819699

residues 248-281 built as polyalanines

1 Trypanosoma brucei brucei LEVO MYVFRFHISSFHSFLVNFLQPSRGVGEPSRLLHHLETHPSYMRRVTSTAVHRTVSSVLSPALLPSFNGTLLLSVCFASCS AAPVRNLYKRLGLDHKATSEEVKAAYRQRALECHPDVVDDNQKAQAEVDFRAVSEAYDVLIDPQKRKEHDKALGLERTVP PAKKQQEGEGVGCDSKGSFNRGVTRPRNRKPFVRGDADRNFREAFHGMSLDQVLFRERLRQRRMQKQMEEKAKEGDDGSP AGREESIRRVAAAAAERFAEKVRRQYGPGMLRHARVYTSLSRDPQPPPSDYMPFRPFHGWTVPNGVRTPPEPTLGPTAKV EDVKDVQLAEPAVGDASHQRKLPKHFPVVQASDGSSLLREETIACMERERRLPHNMGKLYSYHRPY C9ZQR6 ml78 Trypanosoma brucei brucei Lister 427 0.035104535 1 Trypanosoma brucei brucei LEVO MGASAGLIRRGGGVFPDAVSLTLTPSRRVYGGSGRGDLLYENPDARRHSGRALGVLNGVRHSSQATMPESGQLYYRKLIL HSRPPNGSCAGLQRHCHDTCNWSYLIPSLHRCAESAISAKLWEKMCQLGLEDRSKAWVNLTQYERQRVRDGQNLYRYEVH QRLPLLEESIGWAQLDDLLGWFRSARRAWVRLPTSVTLSRESSEAGVASVVSPSSAMSCRLEGHADSRDTTPGRNQVFDT PERVEQLTEATVHRIREELQRLNRSERSDCEGSAAMRASARRLARDEELSRCVEEELGWHGVALQHRIPVPK D0A4P5 ml79 Trypanosoma brucei brucei Lister 427 0.033079617 1 Trypanosoma brucei brucei LEVO MRPTFPALGSRAKGYENRVMVYAHRRHRAWYLPPKLAHARSPLANKSPDEYGNTWDPRTGVEWYHRLRRRGAYRHWPWAR WNDDPVRQHQELSCRRTFSAAVTGANEGVPLWNYYAEVGQEYGLPSHFPLSFMAPFIHQYTSRAWSRKEIERHLKVVEER TGLRTIQQACDATSELLEWGEEEMGVVPHGLLQHVVMLAEDIVLQNKKKAYRKAAHERGILRTTTMERYYALPHLRTGPP MPTTLEQPSGEFPWGKFSTMVGGTRIHPLYRPDGFFKDNMYPA Q381N5 ml80 Trypanosoma brucei brucei Lister 427 0.034883273

residues 145-174 built as polyalanines

1 Trypanosoma brucei brucei LEVO MCCLYTSDVFFWSSLVVSPLPRIVRCVSAPQIRSIPCGSGDFSVMKRSLIARWQSGVHTPHGVVYRGAKMKNWPEQRIPE NFKFTEEQRFRTKAIPRDVGTIPRNFVLGVLYRHQPCEVGGLWEHCTNDPEIVLDSKRHLREVLKQAREEGFVTFERDAI SNEWLCFLTRERYEEVQRIVTAKSEAVDTHSGLRGAAATETSTYAEKFREMNVEAKEAHARRLEEEVANTTRYLRRFQQR EIDYLPYTDLNGKVNFMWWYETRDVQQRADEVLTDSSSSKALAGEEEHKAGSQLEATTASTS Q585A3 ml81 Trypanosoma brucei brucei Lister 427 0.029284466999999998 1 Trypanosoma brucei brucei LEVO MKRLFPSAGVSVVLTSSSIVMSCPCNHIFTSRRAYYWPYNEDFVPEGAETSRFQSSGSPGTRRRVLQEYALSPLFGARVP CCVVGTLRTAKEIIVLKRDIQSLLCELMELPQGSVTLGPLQQLREMMLYRHGTPLSPRLGDERQLNMDAYARRPIAARTM MDVFLAEDMSLDEIVNFGRLSLGKLQIALNNLRKENAEKSSADCEDGSAVEPAQLLVDRMGISYCEIPSLDESDYVFAEV GGVAVTDEEAERVAQRWAERCE D0A755 ml82 Trypanosoma brucei brucei Lister 427 0.029283675999999998

residues 153-162 built as polyalanines

1 Trypanosoma brucei brucei LEVO MRRCAACLTSLPVSSPTGAAVGAPAAPLKTQAPSNRSLLGYPLRRAAAMEMLYGGICIQHLAQPPFPLRKIQSESLPPPS LQGERDDLELEVKDSTGNVMGYRLFPVNIGIRARTESVRVRSEDCYKRFLAQKHCAAAGVPLQFPAPSSITNSNCLATPR AASHFHPPSSSLSLFTRPADSQGGDVGRTTPADVAAYHPRAWRPYQMLKPMPHNWGPAVRSSGVRGPHMQLLQERIDKKG FGWKRKSRSLWQQDISTAGFRPKRYF D0A0S6 Peptidyl-prolyl cis-trans isomerase Trypanosoma brucei brucei Lister 427 0.025579004 1 Trypanosoma brucei brucei LEVO MLHRTRLAQLINGRILQYFVPYERDEVNPVVFMDVTVEGDALGRVSVELFHDIVPKTTENFRSLCTGERGYSQCPLFYKG IPFHRVIPGFIVQGGDILLKDGRGNVSVFGFPFPDESFEGKAGKHLRGTVAMAHSAPNQNGSQFFFNLARNDHLDGKFVV CGQVIDGWEVVDRVAVLSGSSCGTPVSRAWITECGQSSGDKLEDTERALSGERALHTMPGKEVLDILSPRY Q57WF8 5.2.1.8 ml84 Trypanosoma brucei brucei Lister 427 0.024773642999999998 1 Trypanosoma brucei brucei LEVO MLRFTRLFREMAPELQLEYMPVLFTRTILGPQGGFAGEERLVKLEVARKYMEAGHAVTPTEELRRGLWCYNPDTDKYDCF IERNEEFLDFAARKRQWLDVYWRVNTGYLLFGRQSWGQGFLINCPLRKRDVAQKLWEQYKVRIDPRLIEFREKDRRTGIQ ELGHNWCWLYLPGAEELGINREVYDNKRVKVRIHVRKMNSMFALY Q586A6 ml85 Trypanosoma brucei brucei Lister 427 0.022657951 1 Trypanosoma brucei brucei LEVO MRRLGVFCRGQRNSPLLTRGIATGGRVTNEDRRWWLVHLECAPDITPGTFIAWLDCCGTHTCKKLIERNIWTIEQVAALD SDQVDELKYREGCLKMDVVWEHARTIITPLRQREVTGGVESELQGRIMELRKKRELERRREEILKERANVSEQREETLRK LREAIAAKKAAM(UNK)QKKQAASEAYGGSSDGGARKEGAEE Q38FG8 ml86 Trypanosoma brucei brucei Lister 427 0.022376190999999997 1 Trypanosoma brucei brucei LEVO MRRCIPARGGFTMKYKKGTGLWDEDHVNDYKTNRYLSARATMRWYQEMERHQTRNSLNARRATQSHNNNRGLHHTGRGAF ERELERRGVQVEKYPLTTTTGTMRVAELVILRRMELEKRAEEALAEQRGELQKKNPTPSEWYDESKGPLNPNFLRSMRSH YEVDIANLPDTPLIRGQREFFIGEERGNGAA C9ZPU8 ml87 Trypanosoma brucei brucei Lister 427 0.022337521 1 Trypanosoma brucei brucei LEVO MLNPTFSLYRKTLQSYPVPPKIRHYDRRWSGSRTNPYNRQYWRVIMNENYSRPSFWVSDFRHRYLMRTGTDYQGQVPSSP QPGLYQGFSDVHKLLANHPKPQRESRHLPVLPMTPRIVFEHANEKRIDTAKRMRRDRRRIEELKTLEFWGWYMKLQRVRG RWCREQGVSSRGVYGPAVDAAELWG D0A7Z9 ml88 Trypanosoma brucei brucei Lister 427 0.02225559 1 Trypanosoma brucei brucei LEVO MLQINAFKLVRATPFLLKRTGKPADTPDYKQVYLPYDAAPTERELERERRRFKQAYHGRMEHRKLVEVKEVPLNVYTYGK EGMSLPIAIFKDQKDPVIGPEWTYPGIYENKIAAQHWYTEELFDKESKEAFESPWQQQILDNQVKRRMAKVMFRMRQVNM KAVDLFQKERGSSRRSGGAGEKGKDGGGKK C9ZUW3 ml89 Trypanosoma brucei brucei Lister 427 0.022213405999999998 1 Trypanosoma brucei brucei LEVO MSGAFGCGSYRSVVAGTQNVPRRMTFYPSAYELIQLHKAHREVIRHFYVRDKIFDNKFPGNALANGLFKFVPNRRENYHM RELMESIRRRSIWMHRIKQQREINAKVVENMEVKYGKKAAASMLCFTTPDSNAYFAPHRYQDVANSWPNYWQHPSVNHVV PKPRWRRHRELGGITRVEDPFAVQASDY Q57WW5 ml90 Trypanosoma brucei brucei Lister 427 0.022123414 1 Trypanosoma brucei brucei LEVO MLRSRIPCVVVTLVQCRAMHGKPTQSHKVRTQHSRRWWTQSKARHLTAMPHDECKSRPHFPAYNEDVDRPMVVPPDAICF NCDKPIDAGDVNSYVWIPAGNATVPTPQGYFFHFGCFKCNRCKYRLGHNKFYSKDGKAWCIPCALGRDVRVPTRRWHTSY VNTHRTGSRLTGHFFPRHSHQMEFLFDPNS Q585P1 ml91 Trypanosoma brucei brucei Lister 427 0.020618519999999998 1 Trypanosoma brucei brucei LEVO MLFTRCLLAVTTINSSTASAAGRLIRIRKKSKWIDRRSKRIPHNGKDVWQFGEQPSCALCHVRFRFKQDYEAHKESELHQ NRLRWVETMKWWEEIGEPHHQQHAASEWEWFRQRVLPAKAAAMGLSEEDAARELRRAVMHETPRWYSRIQPPNARSEIKE PRDQRWPSSPKW Q580U5 Peptidyl-prolyl cis-trans isomerase Trypanosoma brucei brucei Lister 427 0.020542291

residues 46-64 built as polyalanines

1 Trypanosoma brucei brucei LEVO MRISERAFMDIAIGTKAPRRIVFKLFPRKCPSAVKNFIELCSGNVSTDTYESGNRDKLISESALPQLTYKNSTFHRVEKG YLIQGGDIVTGRGTEQLSIYGGTFSAPEEVRASVFDKPGLVGTASSSPNAHGSQFFILTAKEANHLNGTCICFGQVADGL DVVQEIEQVPIDPSGFPSLKVSIVDCGVLE Q383Y4 5.2.1.8 ml93 Trypanosoma brucei brucei Lister 427 0.018929691 1 Trypanosoma brucei brucei LEVO MFRVTGLQLKNPVVFKQGQGMFSHQLKRLLQKKSIHRYNWDPLPMYDPRKLVHASRHMDVETWREVPDPHWDERSYLVPD QMFYNIPVPPEYKDAYWWRELQARRVQCPVEWVSHRMYNKGDRQRYDFQDLAFRKKFEFSYEEVVKNAKDMRS D0A4T0 ml94 Trypanosoma brucei brucei Lister 427 0.017830958999999997 1 Trypanosoma brucei brucei LEVO MAQWVPKTAWKVSNLNKRYGPGYITSGFSSENTLGYTHMVHDSHKLREKLSSRKGIFVIDVRDPGERKLNPVPRSVALHH HDLLSGASCPILPQQKSAAELFVLASNESRGLNSAAALRRWGYDSVVQVDYNTLVEAGYVSRCDYATAGTKDSAEQAVSG ED Q38EP7 ml95 Trypanosoma brucei brucei Lister 427 0.017803377 1 Trypanosoma brucei brucei LEVO MFRPTTAIADSFKEHYHRVHLPRRLALQRYARQQSLRNAAKGNVKAEEVPYKYNRWWVNEEHEFVHQYAFVEDPEVTKAK RETLPPVTRENIWKEPQQTFFLPFAPYVRVVDYPKDPDAKFLKPVNIPRWKDYMQRTKPVIPRTWY Q389K3 ml96 Trypanosoma brucei brucei Lister 427 0.016387653999999998 1 Trypanosoma brucei brucei LEVO MNDIYARRLAQATMFHQLMRCHGTLWAATQVTKEQMDYNFIREEFMRVNGRRAMPLLLGAAANENLHQLHLSHLSEHCAW GESARALAVQRQTPLSQRVAALGRMAETIHQVKTASTVQNLFNEQISCMEGISSFEEEPLIEGE Q381W9 ml97 Trypanosoma brucei brucei Lister 427 0.012974784999999999 1 Trypanosoma brucei brucei LEVO MSSRFFQKYFIRCGNCQTIQRYAKGYKPIPNPILFDSDAHCRSYHRERRDCTGLTGTLVTCRCDKCARVHSHWTVMDFQE FLDAKLVMTPEERTALLWPGAGSRAEPSSGTSN Q388L8 ml98 Trypanosoma brucei brucei Lister 427 0.012968644 1 Trypanosoma brucei brucei LEVO MVLRGVRLRSVAVSCYGSSLTAATRCLSVRTEDFFSKEAISHARRVSWAPHTTEKKQGAFAKLARSNFGDPLPSSFAQEP YFEEEIEAHRKHHRPDVYIYKYNVSPTHFSLRE Q388M2 ml99 Trypanosoma brucei brucei Lister 427 0.012334969 1 Trypanosoma brucei brucei LEVO MYQRTRFLWSSWRDYPLGSRDRRGRFNMDEAAAALQLNPAYAAALYRPLNYTFHIRGQLYPAQKGRPSRPGSLAASQGRM FPLYQRNDRLDKELFRLNSRGLTTE Q587H8 ml100 Trypanosoma brucei brucei Lister 427 0.010600361999999999 1 Trypanosoma brucei brucei LEVO MALFSCFRCGYMYEFAVSNSYCRKLTLRNDHCPRCDQLTLFRFMSVSGMVGNMPFKPIGVPGPSYATLWWRKTREGKEAS APLDAVCKSDRW A0A1G4HYZ0 UNK Trypanosoma brucei brucei Lister 427 0.003932839