Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete mitoribosome

2024-11-13 REL PDBe PDBe PDBe 2018-08-31 2018-10-03 2018-10-03 2024-11-13 Swiss National Science Foundation Switzerland Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete mitoribosome Ramrath DJF Niemann M mitoribosome, translation, Trypanosoma, large ribosomal subunit, small ribosomal subunit, 12S rRNA, 9S rRNA, ribosomal protein, RIBOSOME Ramrath DJF Niemann M Leibundgut M Bieri P Prange C Horn EK Leitner A Boehringer D Schneider A Ban N Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes. Science US 362 2018 30213880 doi:10.1126/science.aau7735 1095-9203 0038 SCIEAS 6hiv FULLOVERLAP the complete T. brucei mitoribosome with its small and large subunits the complete T. brucei mitoribosome with its small and large subunits 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 Trypanosoma brucei brucei Lister 427 4.5 ms48 Trypanosoma brucei brucei 927/4 GUTat10.1 0.20181118799999997

residues 1470-1487 built as polyalanines

1 Trypanosoma brucei brucei LEVO MMRRVASTGSNGGHCYVGSSLWADSVYMEVSSITLQKRFSFKYATKLQHDEMRQPYYIHEKRYGIFSNERNIAKARRGLP FITPLYTKHMNLWDTDTDASNNRFFRGYYYGQRELHQLLGRPHSLEATNADGSNDLSTYEANTSQLYKGIPRPAITNLHY EPAWRYTLYQAGAHGAQLSNPRSPFTAKVLGDELMQVRDIKSVEHCKAWFDRLQYLINLHYEAVGDIGEFKSRHTRHVHE FFVAFHDALSSLDFRDTYLFDQFKAVRPPELSDLFGIFLEMEANYVHEDYCPRCSLPYSTTRYCGEGDVDTPFRKHRGRW APHQKWGREWYDVVARRAEALWYRATEDPYFGTPQHTQRQAEALLKVYVQAKQRGKAIDFMNKLRGSVEYLTGDITITTV MQESYDALLDTTPHPHLLTNGFTLESDAAKYTGEVNKAPLSPLQFRIDMEMNKYRRQQKEEGVVRVPPALWRLDTSAIVP YKVDSKTRRIVNWREVKEGIEKSFLSVGLPKEAYTGNEWREMLYLHDVIANREAKAAVLEQQQKLDREKLKARERASLKG EKSSNSASNGIGIIFPDKDGYQVFFVDEASLRPFGIGASGTLFKAVARVYPSPAAVPYDDPVHGKQFLIDTTDETCHLFG GFEHGDRLLLRAKSSGGDVDGGNNSNCRNTGSFTDEEEEVIVMGVSTEGSDAERMLCAMHVDTGKQRERGIVFLGTDCID IRERWESVRYAASAYVKGRVTLLESQRTAREEFMGQVVGIRDGVLFVQWRLLKGGGSVLDRSVAVPIGDSTQVRELFVET KVLGVEPLQSPPSWRTPFRNDFAEERLKELQRAPFKREKYVSLIQGKYTPKVKKFGYTQHTTVDDFETKEYKDRLLSKQF FQNPQAFEVVPDRHERSVQFGGKWEYQRTHGLPTVDRNELENGWSEVEPISDGEMKVIEQALRDISGPRPGNFVKPPSKT KSLQLSESWWEPLGYGWEQHNEEQKALCDVTEQRLIDGSNLPFGGKLPPFGTSYGMGERMRAIIEDYSKGFGLGPHGHSP THDTIHYNTLNAEGERVRDLGYTDALGRLFSEKLGDRDVHQWAVESCADGEADVRQLLLSLHEWRERGRPPSLLLANVLS KYLEEEIAAFNKGIPENAPKLQLETSDGTLAHSGSGGSRSGTMWADVDPTTFALHQASQSTRSTRCDEPFILHLVKRAKL GECVTNFTDTAYIAHLESSVINEFHLALTKLVGKGISPTLLAQKTGQLHRGSVRVGGNVVPFVKSRELSRLLERMGLTSE SIAVITRGLANCPEQESVGDDFAVPVSLILSWDGPGSSGGSGSGDRRLNTTAARNEQQRKGSAALSSAIRQLSQHQKQRG SASGRGWQNEDKMVVHVLEELSLRNDGLVMDIQYAIRENKKNRRLRWEFVTTLLPVFGGNEAKVEQLYSDYCDGKYVPNI TVAVEAFIAFLHNATQHPETYSASDYFDIDDGKSSTASGTGDQPSAGQYTTLKLLDPLEGPFVFDNVKVEYIQTVERFRR HGIRAGPVMAPATGFIAANCKSLNYFTRRQEEVVYVTNDSDQGLRRSLENSAYHKTIAANPALQYLLKARRGAALVETFN RFFYRTMPMLNFYQNVLKHYSETIQPLRQAAKSSTRGLARALESERSAAMEEFRRNSERYWRNIIEGRSVEQVVLANGES NRRTVGGGAGEQLQKPQGNRDAVRTNFTGEATDGSRKGQRQHQQPPLSQQQQHSRGEGERSAVHTSPRQGGSRGMADLLS KLNTPKTGSGVKGSTAKNTGNNRRGPKS Q57UJ2 ms51 Trypanosoma brucei brucei 927/4 GUTat10.1 0.09508099199999999 1 Trypanosoma brucei brucei LEVO MLRCTVVGHHRSGKARAFVFRDPSLRMMRAGSGYQQLRRMGMPMQVGMGWRKVDSFHANTQYQHAWPLLSHDDLGNSDQS NNTKNIMYSMYMPKRNKGTAPWFRGADTYSVKYCEQGRYEYQRYLMINRFPSEYKKHFLSFLSNIRMSSGSATIPQEALH WLLRMIVDNFNPQHVHYIAAMKTLQSAGELDMARDVWKIMERQQTWPCTATICAYLDVCVEAGEKTWAMEAWNRYCTELK FLEPGEVDPKPISRVPFSLTREELLYLPKWKKHFDHDPNLDVMDLNRFNRTREVYLRMAQVMLAGGERNAFQHFFTKLEE AMLNKPTPVPEPPNPHLVRRPRWAPYEHCKSVHHSPWRLQNNGRALALGPPVTIEDEMQSRFFSNDQFLVHSVKEVLRIV LQEHKRAHPTECTRCKTEAFFYKTKDADETLKFCDDLIERLFASLGVRLSNLNTSSLLSTILEVFRVVGKESGAALLQRA NEFLERKASLGDAEGSRENLTASNYLQVLSGFADESAFVYNTKKDGTCQYKTGFDPRTTMRHLADVVQEIAGNPHVTWAA DMHLQVVETMVGCGTMKANDYFVRNVLRQFSWDSRFLEALYVEYRRQDDVDMWAELTKRALVWTARYNAPASERLRRLIE DDYDTIRVQTRTFRELAVFQFRDVEERRHSRDVVNELPNPWYDYVAHALPFPDRDAGYPDEYGDLGQWRAPGGPGSPVRG PGYYAPPMEGEHQRGYTAEWRDLRNPMRPPEFPTPWERKYRQYARGQHPSYDMVYAGPMPEIFPMRRDFRKPTRWDFHDI EKQGKYRTSGPY Q385L8 ms56 Trypanosoma brucei brucei 927/4 GUTat10.1 0.047306836 1 Trypanosoma brucei brucei LEVO MRKFCHFMANCWNSARSHATYGAVPLTHSQVTSVYATDGGKVDELGLLELVEERIFSWKLNKWEMRIPPNLPNDQKELIR QEQENLKQILSEWRKCFGALNADILQISSLTGVPKDVVREKNRTWLQEEVAKLRWMGEVNKAALLRDAFMRLEAFGSRDF MFMERLCCIYGLARQGTFDEAFTNYITEDPVTNDIFVDERNPFKELVAHIVRNYSQIDIIYDFLGFNYSEGYRSSLRRYM EYLQCKTAENVRASGRLVTGDKGEHNILFDYCVSRESLVSGDSCQGIIDFLYINGNDVTLIIIASDNPWLRNRQLPHRRQ MEGIARRVCFVLGIPPSEVRIRNLLLPPTYLDKGSIVRLNDIVFRLSNEQSNLLIPWLTNYNKELDPKDVDYTALAKTTN EEEWLTL Q587C2 ms59 Trypanosoma brucei brucei 927/4 GUTat10.1 0.035395676 1 Trypanosoma brucei brucei LEVO MRCSCAFLDKSVFAAKRRVIVPIHPTPNFPAHFIKSAFTTDPLKEKQKARFSSGGEAMREVQDIPKNLEGERSRRDLASR GDTEFQALVEFIEGASYDQLISGRRFKKVYDVLSENDDMFIWLCHTAMSVLNPGDMRSRLIYNHLRILAESVASGEMTQR TAFRFFESAVRSPAYREIAKRQLEGGAATRLAGISAAADVMRRMGLTRRPMSSYFELYQRIVERSEAMTPWGFPPLFQFE ERLSLEPRLKFFSRASQQSLERRRRGHVMTPHTTLHGRRIFWIPPTWNRAGRFLGPHVTLYPGMTPD Q38BS2 ms60 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.034546641 1 Trypanosoma brucei brucei LEVO MEVYRRITERVRGALWAHHGRLMSERQFHRILAFVYNNKYEYQIRFDRMEVVGREWGGRVEVVTTPAGFLKRVRVNPCVE DLTLYQQQQLILSAYANACADGRKLMEQAEMNIYKQFLKDLKPIVMGIRDNPEFYTVSENSIETIGGVLQTGDSNNLCHT HRTIPAAKARQPADEVRARQEWEQKWINSPRGQSWALTLRGKTYFALHGPQYRPRGAPGAKKETLPLDIPAPYTAMDEKR LLKKNWMAYLDNKHVAEVMWTRVKIADREKRQRLLQETGRAWHRPINEEAINRW C9ZSF8 ms61 Trypanosoma brucei brucei 927/4 GUTat10.1 0.03348233999999999 1 Trypanosoma brucei brucei LEVO MLCRTFLRQFRMSGGDMFVEYKVLSRDHRRSIRVEDAIVDPTFKRTVLPLGWLELLRSPSLRLPTGYFVEETVHVSLPNA TSNGGKKEARPQKGGFASGSPSVGRNEANAIIAGPVVLYITGQSVPVVLNPYFVPEGTWDMRTRDGELDLRLGMDAIEQC TLFSELRPGGLLYGKLPENPNVRRNESLRATLGRYGMKCDLAESPLVPRPWTRMRYMFIDELQRGPKLTEFVGHNPRNGT PWRFSQNTKYFRIGIWRDTIRRNDMNEGLHAHSSWQKSPQQSVPEVRFLAPYP Q38D60 ms62 Trypanosoma brucei brucei 927/4 GUTat10.1 0.032068099999999995 1 Trypanosoma brucei brucei LEVO MRRFCTARVSSYVKRAPLLLPRGGRRWRSGAGTTADGGGEKEYIADSFESTAGSNAYAAMELAAEARREIHELWLSAETA LEREKRVQQVAALIEKYKLDPSTPREADVSRGLGDAFDRLLLLCLPLGKTDAKGTDNLERLMHLAGRNGRELSVRTIQHL FARTDSFAEALAVFYTMRRCHVAMNMEAYYSMLYSLQRLEEEGWGQHFRNEYEENGAPSEQAMDFIVKGISNALLPENKP WLGRVMFQDRNVPDRRYDTRDFDELDTAWTQRYKSGTPAGAH Q383D1 ms63 Trypanosoma brucei brucei 927/4 GUTat10.1 0.031922177999999996 1 Trypanosoma brucei brucei LEVO MLHGTPVRRASLRYRRPYWMMFLKGVDNWKIYTVIQQPDHQRTEMLYQAWLGGLDRPYTRPKCMANQPLWLSKKRHMLRK ERLDGPETPLEKYVLEWHKKFHSFQGTERPTPDDLHTALDLVERPLDLSYALQLLGQCRNLNNIRFAKETFLVFLEACLR VGRRDCAEYALEHAEPLGFWFIDEDHRRYLQGEQTWYKLSPLDNLYYPVEENAKLNEGRKPITRLSPATESPGSGTAISD GEPSTDVEGETTVDDEIAQLEAELAALEREGGGK Q38F25 ms64 Trypanosoma brucei brucei 927/4 GUTat10.1 0.030742206999999997 1 Trypanosoma brucei brucei LEVO MLRRVPRVLQKGERGLYFPINHRIVDHRIAPGVTVEETDVQSRYRRELRTNFATGETRQTIAPPWSARERPTHFFSLRLP SRNSVTLRVKEMHNHLLFSHQQCAPMLVSPTKLHITLGVMTIPENSREELVKNIQQSMSETFACVQPLQLRFRGLGTFDF GRVLFIRVVPEADFGRLERAVCDIRRRVGGVLGVDLKGNPHDSYVPHVTVAKIRPNQRQQLGDTIPQSLWADYQHHDFGD VTLSTVDLCRMKGSPDGYYHTEGAVHLS Q382E8 ms65 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.030617381 1 Trypanosoma brucei brucei LEVO MFSESLCILSRRFRYNTKFPALVSYNKLPWEVVNHETPQFHMHVAPHYEQLLTLAASSPVPHIIGSKHIDVPREHRLRLL PGMLYLLDGDTLPGEFTINRVLDPTALQYYGRLSSQIVTVEAVRMLVPDDLRLLCNCITFKGPLHLPVAPYASLASLRGA SQGGTTGSETGSNCFTLYHFVRPNRPPKELQLEKYYIHAPCVAPLSEFASNSDERGNWRPRLQAPKRTRRATPLPAYRPP QSYLMGLAERLAVVPGGCFGRRSLMWGHWF C9ZPP1 ms66 Trypanosoma brucei brucei 927/4 GUTat10.1 0.029960182 1 Trypanosoma brucei brucei LEVO MFNRNFILRAAEFKQKSRWSSVWPNMRYGSMFLSYSVGRKLPMKGVNWVTRDSNRLLNFSNRYSAVISDIDVKRTEEELN VALSDIRWNDHRRIYWKCSFCGSSYRKNVSVRTKYHAGCNFCKKKYPSEVRGEQHGSPPVSQAAPELTEQLVDNGKRDNL ATLAVTSKFYAEWTCRGCGGSYRSTIRSRTGQVEAGQCTLHPDIVAWSAYCPSCSWRPNMVPIAEEVSRSGQFLGLERSM EELGGQRGKQLPIPRRKRLAC Q388L7 ms68 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.026472807 1 Trypanosoma brucei brucei LEVO MRRGALSLLKAGLLGHYQQEAFEARKRFEESTTYPGPIRAATPGDTRFYSGSLESILHDTDRHYWRAVTDDPRVQHLIPL RIRFKIFTWVTSGWEQRMQVVQIMAPKDSTIAQVKDLVIVENQSPYLCVSSFHLAIDGKELDPQKTLGEYGITEQSQIDA IEQNDHLLHRDDERPRDWTVDEITAEDVKRSPYKEMEMQPLQNLAPRYEARPKGYFGRTYYSGMKQSS C9ZKC1 ms73 Trypanosoma brucei brucei 927/4 GUTat10.1 0.011360885 1 Trypanosoma brucei brucei LEVO MLRRSILFRMKYADLELTTRGEFPHGMKEPAFVKKLDQNIPWYFSTYRSMYHWPITGDNWSDLNEAEKHHDLHMFYTLAW WKLGEGIFGVDEDS Q587C4 ms74 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.008203407999999999 1 Trypanosoma brucei LEVO MFSLTQTWLIAHWYCGHKFRHRFMRDKRFHPSLQASHDARNRFSKRRHFKTNRWNYQQAYRDMP D0A3P2 ms49 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.13723412499999998 1 Trypanosoma brucei brucei LEVO MIRRRVCDGARLAPNIRATFSAVRYQSGLHTFIRDSKPSNFSSVRRSENANGDATASPGATAGENPASSGDWASHMQREL FGEVDPLGGQAHKDYYRDVTRGYSPQYAPRNFANGGAVAYPHIQSPYEYEEAAHRRVWLDHDVDRMREEFTQHRASLRSL ASAQEREELLRSRAAEYQVANTVHESESVHPIQQLYNSGGTSRSALKQQAVADRYSIAEQHSPLPLTTGVDRDALDEAQR TKDRILNDSFTAENLLITHGLREKEKHDFTILQRTVRIPFQGYDMDRFLAQQKGTPYGAQQLPPNVVPSSMEEAQRTLRG SSATATPLVDAVAQKVYARNTVVDRPAIGEQLTEQIINIMRASRTTAEQQREEERAQRFGLGRQGALVQDGGPDQRTLKK HTNDERIVDAMLFQQNAYRKTPTDEHWNPYIRRSTENGVGHLLQNKFDIMRREDRLSKGEQDLTERNTIHYGVPIQQIVD EFVFRHRNARGERPLDYFKPFPNFRALRLNRMYRDVEGFSLMKQRPEFLEWELFTRYRQHHQQRRRLALLHGLEPVANET AQERDTRRHRLDEICERTPFDEREMRVNDDEMRVSVETLRSWFGVYMLPSPTVVNAVLGGSASVNLHLYHLADEMGTADT REHVLSSRYLNRLLLLESYQNRVGRGFMNHVVGRAPEPVVPHEQPQEVLRHFSAEERAMYEQHVKEQTSRQLGEWERAMK RRRWLTDHQQYGHVVSHGLETSVVDLSHTETGAVLTVSTKAYEQEIEAVRMKTNATIKVDGMVYNLLPNSERRVVPLTVQ LDSGEKIDMTSEDFDRCELEAFPRNLNHALNYGIANYAYNRGNYVETQDSIWEEQTASGQEGWSPATHADGLREGLPVRA RRPIFSSSAEQRIAGGPQRAVIIQYHHQPFFNPEPRLVKVAFQCDGTIMEVPISDVMIWQRRYHGPERTVGDESRRYNPA AMRRYVDVTDPFNEKTSNTEHFLDKYEPKRNADTVADKYRTTKQITEIDKWTRYDSARADNYRPLSISHRRDYIRMGYIP RYTPWEWIAIQEADQPLIAEQIRQDNIGTSYFFSLNRYWRYKASPHGYIRHFENEVRDLLQYVDGVTPWKQAQKIRTYWE VRSHHPMPQFNRPEVAMHRNTVGLLPAHMWETDKKTGKVKSVKDSVRDYQTKTPYPKWVQL C9ZJE4 ms50 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.13171924999999998 1 Trypanosoma brucei brucei LEVO MFRVRRLAGFIQCSPCGKMGPLKQQTRRYTPIWKSDPAVDNVAPLRDEDERRALWAEVGPISDVGSAVTAWIRFGNDPVL HTAVPTMLGGKFRNQQREKESLLPNSSSPFAYVEDYMGTNLVFGSPVHAKESAAVWATYFERRYASRLRLSRRTVANYVG LINSPEVFDDESDRPETRWSQDTFFRECAYLSEKFLKEKVSNMQQFEAALKRASPEAYLAFFDAFQQQTQTQIPLPSPSV WHYEGERRKQWAEKFISISHKAQAFFKDVLSEDVKKYQEVPGKLLQKVKPVLADVGKILVKRHERWLKGRVWTSLTEEER EAYCMKEVKRQQMQVEDGEFDPMMEDDVDDTELEEWQREHDAIMKLMNSPIDGLHFTTLELWLHTMRCEELETEHIYTSA RVRAIQVAARKKLYDTTSYEEVIQAVVESIARGTLDLGAGVLRPHFNEVWCQLNYAKFGSSTITQHTTTSRRQLLFFHAG SLKDIAATATLYYATKPLSNSLDYASPYKYRRSLITLCSNYGVETAYTTQRPLLRSAANLARAEDLIHAVVTAAAQPFGE RRRAATRDLHMEFQRLAVPVERVIVANPVSALLESGADPDEKPVEGEKVNMWPLGAKRVVLYKWSAPNVEKLKAMESDAA SAVSGSSLTAKRLREIQELKRRGFLEVSLWRRVTAQERKQRNEIVEAKKKQVEEVVRTVPSLAHLHQYATSLYSRIEERV AFPTETSTVTETTNMKEETNKPLEDSEWEFAVLLDDRVLLNKEESVELYLPYRDANGELLAQGEYRALVRAFDLEANPNL HPAYCSVGYSESFQVFDALPQLIAQFFRVKDATAEAAGVTHIPAADFTPFCAFLRDAGLDVPLRCEFEAGQAVTTDGDVY MDYFLQLLRGEAFHQSHAQAGLTEAQRAIEPLCRAHWVVHHPGADESEWATARRSVLDHAMQHEREWWFPNEMLDVKDVV TGSTNGLTPQMYPAAVRYGVELCTVLTAEGKFVDERGSGLSARCVVNGTGAAESVVFDTANCNGTNTTSVEDALRVAHGA LRSAQDRHNTLAAFRLGPLSKQSQVLLFCGVNAYEFGGKYARTYAYAFEKAKKELEATAASGFMAPSLSHEDTERLSDQP TTSPSVDRFASTTHPEQRKAQFVPRVGPGSTPLEDPAADQKSEWS C9ZSK8 mS52 Trypanosoma brucei brucei Lister 427 0.08485108599999999 1 Trypanosoma brucei brucei LEVO MRRCSFASTCRYRSADDVPLGLGSAYVWTCRNITSRGQFNPIHNFSYAMERGVRARDVKAFEKLITNPGPLRVAYTPDYL DWLHRCYKAKGTYMDARAVAEKKFNGNIVSSELSAAVNRREGKRGDTRGDTVDNDHHNLPGAPPPGMFLRPAHSFRRLAG ELKRRRAQSILDEVARAQGMLDLFERQPHFPAIHIDRCSRFHLVELFKEMVLERSLDSNMIWEKALLYRAILSERKPSYP TSFHYIFTAVEDTVFAPTISTPMEMAGSGGEGHDRSSHPLAAKCPTLEAYYYYVYLVKKYYIDNAVEAHVVLRCHREPNA ADLLFSNPPPKDDTEIMKAVELLRNADIQRGVAAAAAVSDPTLPPGGEGSVIGNSDN(UNK)KNSEK(UNK)SEGSRGRP ARPPVLPGAYPPIDMLWRCEENLPLLKVLLFGEFNLIVSENPFVKFPSAHGFLTRPYSTDSSRTLADGMSLANVMAEKRG HLLPSLPRNTATSIDARAQDIRRLQQKHHRDDIVSFQKLLRTHAEDSPSAFSSYSDWSYFNPRAVRAEERDRLTRKAVEA LKLYDSATNDIYRHSFEDVQACHTQRVTERDRTMPPYLPTLPHFVAIIKKDPHISFLLHIGLPDRNSSEEGSAKHKELEK RIYYLARALYHTALEYHNETVRRVNRQKVNVAASLLDNFVEQEWTTILRDKHDVTDVTKTLNDTQNDKKQLARRLGRYML FANRSLDDTGFPTDARADDYTRWMAPPSVGKVSL Q386Q7 ms53 Trypanosoma brucei brucei 927/4 GUTat10.1 0.075338914 1 Trypanosoma brucei brucei LEVO MSVSGVFSKGRGIGHEATTSILRYIPRARVPWQPSRFGRENLTAADMARLWSRGRYRDGPGNYNSGYCTERTHVLEENTV SIIPRRELEKYMPDITIGPKALVTPVSLMNARNGHRVTHDLLHSYDPHIGRLGKPAVVDHDNITVEDPNRVGLNAATLDC RGRIYRWLRRGPFFQVDNYFRRSVKLNRDGTLPTDFVHEAPLMRKIIRLAHRGHLKAACEEYRRVTTVPPVEVYRALTAC CVPGAKLADAVSIFEDGDSKLFYVSRDGEVLHNLMRCAIAARHRARIMWVYNVMRGRFYENVVVRAEVDLIWRYRIAMIA LEYLLDHECAEEAAAIYSYLVEEELLRCDVHVRVGLHMREAIAAGKPITLNNDVMNATSLVRDATAVAPEVARELQRRHA QTLQNNAVEAVGAENVREGSAPWSILGPLTAIGPTAEDTMVWLQQHYGDVDVMSIMRWARFRKGKDLMAKDRPQYLARAA AWIELLSKRNREMEEVPLTYMRKSKPLVLDTNSNVRVAWQTPLMRSGGPPRLLAREEGYVFHHSNSSRFVEETYRHPGES LQSRYLALQPLHTEVSAKEDFQRLYYQAQKHHKQQERLLPVSTAAIPSRIVHHSVMSALHGVSGKGEPANRSLFT(UNK) NKSNDSHSNTGVASGTSACTDGAGSATPEF Q38ET1 ms54 Trypanosoma brucei brucei 927/4 GUTat10.1 0.072046031 1 Trypanosoma brucei brucei LEVO MFRRAIPLLSANIPRSVWDPAQHNPNWSDSYGHDITNRRAWPARKWTVGLEPCTPREWLQFSHRNLAYAYNGALRACHSL PSMLLLYKEMKQRGVKVDVDTMNVLLTRAARHEHIQVDDVFLLFDELVALGARPDLAAAETLHTVLSHSASMPEEWREAR RLQLVELYNNLAMEEVERLAPHRADRLLKEQMKRFRGNLQQLGSGLRPTVYCRYLHTTHTAAVLLEEVHNFLWELVPNDH PAMEIPALQLRVPFVASVLRRPSVNPGVSLASVSRAEFGDTDVCAVFLAAAERMVDADFDDQRPVSERRLFLSLLTMISY SGVLYTSDLMAQLMEMVKYSNNDETRDSDAQRVLRYALRGSSAAQDSASRTLWHSVEKVADCRVVGRYIGARNPWNPIRV CFDEQGVFKAYPISTTTTTREVSPPEGNGAVTQEQRASCVEGRTLEALNMRWDDVRRLIECTGVLVTPPSERCPQQQKME VFTGMAVYLRTVATGRRYEGGEDVLSDGAVATSSCEQRRRGTLFAEGYDFDVWVRLFSLVQEVRHDMEKFMADHTLQCVE PEFECWEALLVTLRCALDFCVVQMQGGGARGTEREVVERLFRDVVALREELIEESRTRFGGRMRVLWLQEA Q57ZP8 ms55 Trypanosoma brucei brucei 927/4 GUTat10.1 0.06667667999999999 1 Trypanosoma brucei brucei LEVO MLSQNVAKTTVPSYYMIRTNLPHRKPQNQWEGVYYYSGITKRQRHLILLHRKREREAHMRSFNISRASVLQRLEQLSGDR KQESLPPHVRLDLAVRLAQHGLYQQATPIVDELHHQKALHAGHYALLINALACPRLGQRILHCDAQCDPALTYKLLGDEN GEERAQEAYRWFDLALTSLAVDCGGRTQPSHFVPYLPQGTAAASHITNALMRTLLTCGYTHVAAIPDSVYDRMGSMGISP TISTYELVMLALSLQGNMVEAESILSFLRSHHSEHITVESFNALLLGHREARQFDCCDAIWQELVDRRWPRASPLTAELY LRSIMDHANTPTSEPLQSFANINVVEKKKVPLVLAQMDELGVPRTHLSRVLMDEVEDSLRKFQTYRSRFYEWGRAVKQFD FIEFRRRNGWLYDLHLMKCTTKQVGPLRDFNDPDAVQGAVATAEIPAFFNERPAWERPPLEETLYVTTNKERYDDVRGGD IYYDDTRGLHDRSPTWMNEVPETRYDRLYGVNHPDIAKIGIRRHLNVEYVNRKEVVERDAALMKKTLSSGRRLRHRVESS RTHRNAGSLSGISSTAGGGSR Q580V1 ms57 Trypanosoma brucei brucei 927/4 GUTat10.1 0.045956292999999995 1 Trypanosoma brucei brucei LEVO MFRSTRPRSVGYTPVNPDTSPMVAYSQYHWHYNLPQGMERPHSVNRTFAAPFQSNHSLVNKYRGVWIEFDMHPAFSVALE PQLRKLPRGRTLPKTPAEEVIADYTALAPLVDDEKTRDLWLAKVFQHCAFQRCGGAMELWERYCHQRFTAEGATAKPPLS LVKSVLFYCNKTDNSGWRALFDRCLKDGWNYTPLFDTAQWSFMLKSIGRMGDEDGVRAVLEEMLDVQADLDRVEARSVVI ALNAVTNADVYEFVKKYLFNFGERKVKFLRTTYSDLRGHGAGKLRIPLKENDNMYYHVCWHSSIRSPRQFSPRQLYFDYT PSTLGSSSHNPNAKIDDIVKDKIEKWKAEGLLPEDYVHEDRVYDRSAAFKNVARQEKWKKMPKILKSKRMGYTGDP Q584U8 ms58 Trypanosoma brucei brucei 927/4 GUTat10.1 0.03562391 1 Trypanosoma brucei brucei LEVO MSFRYTNNLIGALKHRLLLESSYREIASRKFIGNCRGVEVVCSGYGTVLAVQLTDKAVWESFYRKGGRPTVSGGGDVSGD AETGTQGSATTTGATGSLDLDKLAESIKTALWDATRKIRSAKEAALHRSLSHNTRMRASADLKHWYEEDANTLRPLAFEA LKHEAATPWMQLVQHGKKEEAAALLKEFEQKGDAAEATPTRVKDDRVKGTRTELSNREQPPLASTLKAEDSNPATIPIGS VHPLFTPALVQIEEAGGGSVSNEAVCRAQLWELSRDEQLFWERVELIRKGQVASIGSSHKRGYADEAAFAKDDTEEKVQL RFTQ Q38BP1 ms67 Trypanosoma brucei brucei 927/4 GUTat10.1 0.030096051 1 Trypanosoma brucei brucei LEVO MLVSYLRLQADRRTRHHMMVDGQINPREWFNWHPKEYQPWYFDRHKFLYEQRQYAQFHHLLCHQVYLKDMLHLMRYPEPY THIIDCRTSTQKMHRWVPHSLWLPRDEVEYALQLSADEFLDMYGFRKPDKSDDVILLSHNGIYSEQAGWEWKKQFFQHVY NYRGGTNELFGESYSDMNVSDVLSPWKGPFPQSGVFVDKWSKRKVLTRTGPFDRQYEMQDFALPDLELEKARHPEEGPRQ NMPFGLQ Q586G5 ms69 Trypanosoma brucei brucei 927/4 GUTat10.1 0.021589732 1 Trypanosoma brucei brucei LEVO MRRVRDGLFLSHPSSALQCSVAVVSTGEFDHPPFQFRQRHTFNTTPLHDANRFGGRTAYLREIGPVNIKKQGRRFKKDPR TVQFNVDVWCAQQTLRKRWKQRDWEVIEIPFRLVPREQQRVIPELYTDIPQMTDPARNDFSNIRNKVYDREELQGVLFPA AGAMLYPPLQRVDKQAMTLDKYL Q57UZ6 ms70 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.021247398 1 Trypanosoma brucei brucei LEVO MLRFTHRALTATPERFSVLGTTHPKPKRTGFGRNNKMRSKPSDNVAWYDKGPVEWLPRPVRLTYDHLDQLQQWTMRATLD GRTEEFNRIRDLHREWSQHPLMPVLGDVEPKFPLNLFKQNHRAKKRFLVRWHKANTPANWLWMPRGPTVVTPLHRTNPTQ YPENWKQMVQRKSGTGTPS D0A8P6 ms71 Trypanosoma brucei brucei 927/4 GUTat10.1 0.020093226999999998 1 Trypanosoma brucei brucei LEVO MFHRAFVSSSDLTGCTIALSSVCTQKRYWAKPKKRPKVGQGFHEKAQKWREEYLLDRHRALADSLRAYVEFSTSKRVEPW DARFKPFDRIEKDGVYVLMRYMMEEKLQLCNYHHRPVKRLFCNIGLMGPQITTKARWKPYRFATNPAGTSKAERMYQRDK TVYTHGHND Q383G5 ms72 Trypanosoma brucei brucei 927/4 GUTat10.1 0.019317293 1 Trypanosoma brucei brucei LEVO MLRSTLTSLNTFLTSSVATPPISVIRTGPKWWADPERMVRQKLMYFTLGVDQLPLRRTAVIQRDLQRFHMCKPPPRVGDS TGYKRSRAAQLNTWYRRIQYQEYHMQHLFTRHVWGLLRVYPGNTTKIQGKADDGYVGYDSVPFHRYNRAPLPFPARELYE RRK Q57YD4 uS3m Trypanosoma brucei brucei 0.00907094

Mitochondrial gene MURF-5

1 Trypanosoma brucei brucei LEVO MFLIHFVHYKTILQKYTFKFKHIFLSIDKYNSLFFNISGILIWLNIIHINIILIKYSFFILINNFEYLIILIST E0A3K1 uS55m Trypanosoma brucei brucei 927/4 GUTat10.1 0.050147355 1 Trypanosoma brucei brucei LEVO MFRLTAASQKNLNSWYTKGTMRGGVPRIYYAWMRPGSFTRRRFEKMRNPFVDLETGTSLYFRDTRDSAEAIAHAADSKGI KGMDNAIDLYNEYRIVPDLYPEGFQWKHKLNTEYNQWRSNTWLTPDLIPKEHRGRFLCNFQLNIVAYDMRVVKFSPKDHR QWIYCVLYVGSGKGIAGWGRAVAPSTQEAKKEAIREAFSNIIAVDLEQEGPMYPVRVNADGVRVLLYPARRIVANFRVAD ILCAFGFQHAGCRINLKATNNPKSPTHTVEGVFEAVKALRSVSEIAASRGKVPHSLIYNIYPYLEEIRRRKGMMAMHPPG KDGLLMPDRVVDNRLPDHLKRGYYDDVYWKDFFAGSDEHLNEPRMGLRGDEMRRRLEEAQTSPAPTTAKDTRRRTLEDVL KRLGKTTRDLGSIPIVNPRVDVGLPTHIKRNYQLH Q38AX6 bS6m Trypanosoma brucei brucei 927/4 GUTat10.1 0.018861543

residues 105-114 built as polyalanines

1 Trypanosoma brucei brucei LEVO MVFYSFVLVMKPRQRRFTSQALREIGVAVYSNGGLIRSITNEGIMRPYSRFRDADNTPLTYARYIILQLDMGEEEMGKVD KIIREHQDVLMALKLNNLERPVGIRSGNKELQAAYFPLDTFTRLEEEINWSPQTSADIYTQLEMNWKEFSRTRWSSFLRN Q38BW5 uS8m Trypanosoma brucei brucei 927/4 GUTat10.1 0.032766459 1 Trypanosoma brucei brucei LEVO MLRQSLLSLAKVYGAVPPLGAAQGHRLLHGKREREGSLFAVANDVKRDERLLRQQLNALLEEERMPMQARLQRNEAVSSH EAVPQTPLVDLPGVERRRDLPADPITRLFFQHKGDHALYYGTYDKPSVQDDDRVQIEKRVPRRREENLYTPIYDFCHRIR EATEQRKRFVVVPSTIETRGCARVMHDHGLVAGFRDFHNDRAFAVELKYFQGDSTINVIEPCSYDGRTEFEWSPKMMRRL LNTHGIHNRLVVYICRTADNRIIDHIHAVKENIGGRGLMMVH Q388R7 uS9m Trypanosoma brucei brucei 927/4 GUTat10.1 0.050201016 1 Trypanosoma brucei brucei LEVO MQKLLSPRTARHARLFRLAGKLADSGSPGVPKSDGERLVWVNSHVRRDKDISLSQEEERIRELMMPLEVGENSFAANGQA THGNLFYFREYPMYPGEYVPAEHNTLSSLRDELRLDLTAQSLKEAWMRVSGGVYFQSVDEYYASVDGLDAEQIGEVLAAL FPELNCYEAQALVQRTLECISRPVSAASRQLSRTITAEAVGLDNAPGHYTNFLEWMGRLTETRAFKTEHALFEFSRRKFN RDDVRVMFENYRLMSKATLLADSADSYSHFYTVLKDFARKVAGEDSRHQIGVRIDEAEVDPETGIAVGRGCADGEKYHFT ALLRENRDHNGIITVMGKPLSLVLDNKAWLMEMVLMPFDEANLDYRDFDAHIVSEGHAMPSIANEIAAFALRMAVANALV KLIPLTRIPLKKSGLLSVDRRRERGQFPGYLDGKKVKRRFAKR Q57W62 uS10m Trypanosoma brucei brucei 927/4 GUTat10.1 0.094351039 1 Trypanosoma brucei brucei LEVO MVRRSHVVAYYWSRYRMPTQMPKFDGPAPVAAPQSMNSTKTNEFIDPIDDKFPMSIRGPLVRPDVPEDQYVDSWYICTSM THHMGDYRPWSASAPPNAFRFRPFNEFDAKGREYVQYMREFARFDPRKSRGNGQKGFPFRDAYLTKMNEANQKTPPPTLE TIMDRAVREHHQHARILSPLEVQRDVGRLEPIPSYAGKINADRSVFPFQWKTEDWYEYEVAKVRNRRFVFENTEEDGIRG SEVTYKIVLEGFWDHHVMKLAEDVCMFLKDVGRQIVEEKLVAVRRLLQGGAVDPELLAAFNCARAGPFGGLDEYDKEEVA NFLRSDLRRLEEQCLSVINRCNVPVPGATNIYDPHTSWPHVEKLEPWVRMAEFWTSSSDTSFTELEMSTAHYEFRKFFRV IICKLPFQSTEFEKRMYDIRHWLHRQTSCEFHTIYRRNVIHDSAVFPTEHDPATPTTHEHHRMFSFALDWQSAPVNRLST DTVHEGESWDAVAQRLGCSVGELKDANAERETIEAGVVINVPVTATRRLTSFGATPLVLPLKTTSAKDGERIRTWEEAAA ILDCTVEELQQCNGHAALTYQKKESEAGEFDSSETELVAPLSCWTSTSESEFSPVERVHANDTLVAIARRLQCSEEALRA VNDGITDVSGLDFVRVPPEARRPRRLVEPQLRPQAATDALLARTIAEEETFKLKSIPHLPQNAERFPHEYHTPTSRFPPT PSETPATQDWMAYTAKYLDKQFTISAEPAPVYNVNKLWPMQQIPGKVDQTPFEEDQTWLLHSIPVQQLEMHHHEKDLQDL PFINHEQFPRSLEWNAP Q57Z45 uS11m Trypanosoma brucei brucei 927/4 GUTat10.1 0.03792591 1 Trypanosoma brucei brucei LEVO MMRKSVTFLRRGKPRPRAGMFPDKYRRVPMLLKPQQGGQQYFNHFLIRSTNDRLTQQDVDNASGQAHSFISPQLPQMDWR NMSARSSEESIREEMRQLAENDVMQHQRVFNERMWYEKEEEHRMKARSCPEETSEHA(UNK)SNDGAVPPPRVLGGDYFK TRFGYSLVKNSEMTQGPVDYSQLDMWGEMPRYTSDMVFLYLVSRRRNTYAVAYTYEGKRILNTYTAGNRGLKGGDRGFRS EGSTDNGHQVTSMYLNDLLPKLREMRASEGRPMGRGEKVELVVRVMGFYNGRQGAVRAVQDRANEFHVRYFEDITPFPLN GPKMPRGVFK Q389T7 uS12 Trypanosoma brucei brucei 0.010421635

Mitochondrial gene subject to RNA editing alternative start codon

1 Trypanosoma brucei brucei LEVO LFFLRMWFLYGCCLRFVLFVLCYYMSPRLPSSGNRRVLYAVFYLYNFVWMLRCFFCCFIGLVMSLFIIEGGGFVDLPGVK YYTRIVS A0A0E3J9R6 uS14m Trypanosoma brucei brucei 927/4 GUTat10.1 0.019900013999999997 1 Trypanosoma brucei brucei LEVO MFCFSRGLWMRHIGQDVPKRHTHFVLESRLMYEKSFRDCWLHSVCRAISQLDEPLSKTVVGTHQKMLQRKVTCFQYNQYG LFKTPYYRLANVDRYHAVQGVAGTREWVPYVNVSYWTMNKMVRGGNLLVHRVHYTGWGTDSHLKKGGWEHRWNKVLQRNV LQYSRI Q580I0 uS15m Trypanosoma brucei brucei 927/4 GUTat10.1 0.050401191 1 Trypanosoma brucei brucei LEVO MFVRSFCFLRKYGSKSKTPDLKTSFTKTTNFRKAEKISITTTDGEIVKGRGKIRRRTHSALKEAIGAMEHPAIWLWYPWR MNPEPPTPHMPQRRALKNVHGAVFNDLTPVQKKRQEQMLYGVNIPETRQMKFEEQHPLLAGALRKLEGQPKGFPFWYRKY PTRRHAYEYRFSIPVEMLDGYNDDVKKALSKGMMSIQEKQFAQEAMYMERYAEHDFDTTSPAVLAVKRALKCRVLRNHLL TNPHNNIIKTVLANTERKLNHALRRLRKVDFKKYWEIIRDHDVQDILQPPNLVTYRQGSYWKYDWNAGLAISTNLADVMD PRGLNGCVETGRSRSEVARDLGLSYTRPLHENEKKQLSHQAVYYERLAKFKMEQPEAARAMERERFVRKFSGMFVKMDIR SGAPDFPSTYRRLLGTKVVRWASKRHGPN Q4GZ99 bS16m Trypanosoma brucei brucei 927/4 GUTat10.1 0.022077805 1 Trypanosoma brucei brucei LEVO MQVTETVLARAVIKRRSPQLWGAPGAPIIRMRGHHVVWKFQSYDLVVEHTHKRRNSDIRLLHYLGKHCPHPQKSLWSPDT PVAQDRHLFMLTTVDIDAFKYWFGVKRCRLSMKPWALLAKAGLLPPSLTQNSKIMPKPLFDKESLMRYYLANRKDEDVMA REKYLNYENSMVKTEEERAAERPVAPYL Q384N9 uS17m Trypanosoma brucei brucei 927/4 GUTat10.1 0.035632726999999996 1 Trypanosoma brucei brucei LEVO MLRRSLLAFPWWNFQTEHRQRCVLMYGGARTKNTHNANHRVFIKKYKRNAFPNRTRHHWAVSMTGVLSQRPRRMPWPYDL TSLIFNQPRQGSDKIGYVVGTSMLKTAVVATNHMVYYPKFNQRVSRTKRFFAHDEDLACVEGDLVHIKQCRKISKYKHYY VFSILEPNVEGRERLKLGLKAVPPPLFGYPVSRRIVKLNLTSTEGTQEKLAAAIQEHVQDAYRFSGPTPDQPRNRLADPV TFEDANNMIAPNAPAAAALDASDSPPLLDRGEYTEVEQDTRNKKGDDYWMNLQPKEKYDFKSFKKSP Q38DP8 bS18m Trypanosoma brucei brucei 927/4 GUTat10.1 0.036988691 1 Trypanosoma brucei brucei LEVO MNRTGGSIYAHALSQFAVCRQPWNEYIGLLTKQDSTPYHTEPQEKPAYRGRKQGREGWLFGQQVQLHYHRFPDEQLITNL SRWRTGETVGDIAMQQFRNAQPFDIEDKDPQGVQRPSPEVYMKLNYKNPATISRFLTRTGHMYPADILPLNPEAVVKLRV AKAQAIRIGLYPRFGNPFWFRSQKFRPKAYQENYDPTTYSTKRTVEHFAYNWVQTDRIRRYFRELEAVHTSGSASARGSG GGTTAEHKQQDQFYSPENQPISLHRNNISYMEDVGRSVKNPTVPGLMSTKGMKKKFHNLYSSTSTKRMGFSNPTLGIKKV Q38AS2 uS19m Trypanosoma brucei brucei 927/4 GUTat10.1 0.028364603999999998 1 Trypanosoma brucei brucei LEVO MAFRNTFTTPGKFSTVSKNIVLLLIWRVKVFLRAEGFAHSLVMLPVSLYSKILLCDVKKKIVYFHCCTRKKSMLRRCPCV WPDGPTKSSVSIGTAFTLQKRFLKIAKSAFGFYLARRGQRKYPFLRRPHIKNTHSMNPSAPYFWSFMTAKSQMAFLPEEN YITGDWTGKFFVSKRQVYTLQHATSGAKVRVKSFPSIFEFNSPSRWNIGKEMNTLTKPRMDLIDEQMLTKKQRLDYVRAG LLPK Q584T8 bS12m Trypanosoma brucei brucei 927/4 GUTat10.1 0.023087888999999997 1 Trypanosoma brucei brucei LEVO MLHTTRLWLGGYMMYHRKAMGTMKYSKWKGAHGGISHFYGRTPMVEEVRPNEPITLVDRRIMHYVHHSRLRHFQLFRSYQ EKSNSTECKLREGEMLRRRWHRRLQKSFIAFMQFKTMKVLEDQARLVNTYGQAAVNAALGDPWNATDNVARERKSAAVRR QVRALPMVNVVPKHVATMKQIHNDRFNYRWRVN Q580M9 mt-IF-3 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.041086285

residues 294-304 built as polyalanines

1 Trypanosoma brucei brucei LEVO MMQRCSTSLSRLCFRRLLRTPLLVYSIPPTRDVPSGIAHCPLSCSMRMVTSSNDDEFVFDPTLSIQKDAAIHTAKKSFET IVLEYVPAHAPEEARQKVKSYLTQHPIDILITQPKVQITHLEDAESGAETKVSLSPCDLPEALQQARERGMNLVQMGARG DVAYCRIRRESTRILSLIHTELEALREQEEKQQGKGRGGVQAAAKMGELIDHTFRDAVDAHFVGWRSKKIVEDIRRRHPV KLTIKEFQSPECAIGKLREMCQAMQHYAQEKVIYHHFTSIVANDREASITFAPALPMAKSDSWKHIKYPGEKEWTNALRR MEDACRKSGRYGTYAKSNKLKLRSLGQTSYRVDKYGRKMD C9ZRZ4 mS22 Trypanosoma brucei brucei 927/4 GUTat10.1 0.073064141

residues 20-37 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLRRAYIQRRYPFNKRGPREHKSWKHHVLTEPPKPLQWRDPKVWTRDLSVMKSFDAPQWDLWQSRPRSEDMDEALQPFMD MPKSLKDRRYDIPWWANPFGAWYLQNILSLELLKLKSKTNAEKIATYRSYMRSLASGKDNTMSDDDVIRNIIKERWKTLE FGDRNAGYPCTFGDYIQFLNEWFKSLDEEGMQRLREHFDRRIRPLLAVMSPVDILWLEALTQNSPHNKEQLQRKIAFQTS LGTPEFFDMSKRLRYEINEDYKVRDELGPELFALWSKAPERWPPERLSKMYGLDFTLVRKILVWHHFKACYDACVEPDWS LPKRLFALEWIRDVRARKHGLFYGKMRFAEQKITFYSDRFLFRDLVNRREASYANVWEMDDPYRFLQTEQDYEDYWGDNY DVYRRMFPEMIGRTGEPVQQYGQMPIWAGPHRQHANKSEHNWMFAEIGVNVGHEALKKLELDPTNEKRRRFVIRQPDGTL RSAKMSEMRAWYWKEEWADFRFWAPQMEWGIENTPSQEQYQEHVPDTTDADFRKQRRIQSRPVKWFYESHYTRTGSFAGF QPLRFMQRRTEREVRWPDVINAAVQIQKRKPAAYIFKAIPEL Q38DR3 mS23 Trypanosoma brucei brucei 927/4 GUTat10.1 0.0367845 1 Trypanosoma brucei brucei LEVO MRQCVVRRYKMPKNMGVAPRFDTWNEKYEPWEHMKRMGRLVGTGFYIPPEWYNHFRMFPPINHNFQQEKTLNPHNASEPT QDDTSTLSPERVALRDELARKSRLVASEGMRYYNIFWVRKPLDTMEKEYYELKRRGVDHGEAIRKVLQGFYSGLAVKKRV AAIQAEEAKLTGRFITMREATVVLGVLAKLHKEQLTPHQVSLLAKEQGETTQSGAKLTAIVSRTQPHVNKEASSPATSEA VGSSTEESLSADALASMLSEDGEQSAVGTRYQVEVKETANDSVRQLREKAEDQTGFPDWYTGESPTYSGTSCSRDGFALM KANK Q57VB2 mS26 Trypanosoma brucei brucei 927/4 GUTat10.1 0.051136684

residues 221-248 built as polyalanines

1 Trypanosoma brucei brucei LEVO MMRSSSFCRRQIRPYYNLPSKSEHGRKMTGFLTPYRHWMWKQNELWRNVHEAQFEHLRRVYKRQWLESFRVNADEYIYKY NITKAAQLAQWECEMKEQEKKRIEARQMMDGRQALKKKHLDLLREFHERQFFFWYERASERLQNMNLINYVPHAQLREHI DKELDKYVAGKNEPYPLNFVGQMPFLEDGDGNIVEVPESLLSNHMAEHPDSTAKPHEPHTSSSISEAAAFEERMLRAMVS AKEEDLKEWLGDDSRALSETIDDISREEEEREADIRVARSMEETDAEREVSRRAYIERGKTGSRSIFRPPTVSEGAGGTP SAPAGDANTPMRRRKKGKLDRVHALQAHQDELLAKLSSQGLKEGVDASSVPERGKIVQSRGRIRDKAVIPTHEVLMQKPE LAAGSTPGARIQTKDMVDKMYHRGKYKKSGSGDKSDGEDL Q38DK6 mS29 Trypanosoma brucei brucei 927/4 GUTat10.1 0.057048836 1 Trypanosoma brucei brucei LEVO MRSKTLKKSLAAMYMRPPVTCYTDACEAPVAMWDGAIPLKETRKLKNGVPVRTVSRTYSHPPQLTPTQLSFNDINSMYCV GNDELIQFFPEGLGGRVFQTMPPGHPRGFLYRKETHLLNLFVDKVQHWHTKRSVLSSLTNGRTGFIVDGPTGCGKSALMC QVVHFARSRNIVTLYVPDAKVWTHGEWCWPSTILPGFFDAPDAARSFLKYFAVANRATLTSWKLRCTPKDLPTEQGERQP QNLYELCEWGHRAVAPASIDRQSVCVKFLMDELSEEKKLPVVIVVDGWNLFSHETHFRYPHPDFLRGLASFNESSTDIDL YPQELPRIPASRLSFVRGLNKMILSGDDPNKFFITCTTRDFKPFDGISGFPNVETDRFANSLDEYAPYDPEKDSHFHPIQ IGNFDEYEYRSFLRFLINSGELAGLGWGPLWHASSDFERKLYKIGFLSGRNPQGVVDHYHQELVWRYDYQRTRQKQYLLK RRMEGMSRGASSGAVGVR Q585C2 mS33 Trypanosoma brucei brucei 927/4 GUTat10.1 0.021244104 1 Trypanosoma brucei brucei LEVO MVLRRWFPLLGVHRVGYTHPSTLPVPCAQRWDLRLARARIFQEYIEEKAPGAWQLEDERSMSPEFKTFTGYPMRDMRPGY GQNLPDYIMKKRLPNNTHYELFARRDIPNEDNAMYGKYLYDMTVHGTSLPSTYRMHKDINKAQRNDRKLSGNRFKVLCSS GAKNPPSGWEPIPDATEEEED Q57WW0 mS34 Trypanosoma brucei brucei 927/4 GUTat10.1 0.02872818 1 Trypanosoma brucei brucei LEVO MLRCARVALRADPLNGGSSMTLGSKGSKLSPEPHRRRMPWTAAKEYVPGVVLNARDKMVLDGVQLLDIESIDRASQLDPL EVLRAVVATREYNISTGKNIFQLASQATYNGRGQRFYRKEWQEGTYDKYVTLSAIDFDRDGNKGTAYGYITFHGETTTRP VQVDFADVPGWYMDFVEERAVPFTGIVPPPPSIGTDVPVDPHSYRLKAYPYYDAPNPPEFVERLLKDRGVLPDTPTETAD VDKDPTTSDGSVHYDGK Q57UK0 mS35 Trypanosoma brucei brucei 927/4 GUTat10.1 0.098377773 1 Trypanosoma brucei brucei LEVO MRRWHRSLNPALFGERGSGHYFSTTRARLQLQAQDRIICGTHGAGIVAHQTAVNSSDNPMLSSVTTAAPAADLSRTKPHE GTGTSERDPYIRTLHNQRSAAPESSVSQSHTVNAPTVDECEMLAERWGTMNYWHNDTFPRLVVFLKKLLVPDVSPLSPTA ESLLSMFEKVVIPKLTSDEEDRRKLVSLWSETTLQAEAAVTKFLFQRGSFESMLHRIITDALEKMSTLALGGQEGNLALE ALKRQTLFKRNDYIQKRLIDVVSNSAYLGYGDSVWQVFFAAVEANEENLLSDRATTDAIRAAWEGVMREDVVRLPDVTGV VALYLTLVCIRESGRLVPEELKELSSGLEDGVRPGVRKLQQYPLIFLHPTVKRRFVVKAVAEILHNSSSNAFSNMLRENG LHDTAREVALCEAMNRNKELAEGDVGDAVGRFVSKGEVKTLLSSLVSGTDAVVRDAVAGIFGIGTTITIDWDAVMQNVDW SNNWQRLATALLSNSAVLSAIVKLVKNAIGAKGMSKHLFTDEYADQLQLILDAREERAASRKQRIENIAQELSSFERVDL SCDLLRKLGVDMTELDTAAAATRNMNVVQRPCIEDGLLSLVLEAVTKRHPNWVKAGVIQTTLKDPFDALRWMMHIFIRLS YVPHAGAATIARLSRRRIGPIGLEPHQFNVPAELGFVEQYDNLQYKRYDWQGWYQRMLDVHNRNVSLRCRICDLQRLDGN GVQFVDMQTERRLRILAQHRVGMGVLKLDADKYEDQADNVTFGTTKLSELLADARKAQLGEEYWPSVELKVRKPSGQSKA HYSLIDNERIEKRSRELYEKYRDAKKRSLFVTPMETWLEVKGMQVRKSVDNADEDGYTLDALQDMMDGDDGDKV Q387C7 mS37 Trypanosoma brucei brucei 927/4 GUTat10.1 0.024757895999999998 1 Trypanosoma brucei brucei LEVO MKSSDIFFAYRLTPVVFKSRQHDSGVNQYGLKPTNAYDYINPTNLINFGRGTTFDNLGVRRAGRGEIDSSPSHSGSPVFT QAKLIGLSGEEQLTMCQSETMALRLCMAKAGKETCERESRALDSCLGRVGHLRRAMSEACWEFNDWFIQNVSDNHTKPFQ HRPHDWRHFYAQEKLVRERQQNGHAYGRRPKQFSFGARYVKTDGYGKRPRLPYNK Q38C96 mS38 Trypanosoma brucei brucei 927/4 GUTat10.1 0.028240599999999998 1 Trypanosoma brucei brucei LEVO MRAGCVACSRIPKLGIAALGSTTTSMQATCQETQELRCATTQMAPAVIFPLPPPLRGSYIDRKPTAASFNEHHATASFRH HMIASADVSHRPRHFYFASGTRNDTGTRSVKEGERKQLKQQTNVEVDSVAYGDQLDELSARWAAKFYGQVTFGPRNYPYP SSRWLARRFQMKKHRIIKRFRFRRYKLAAVANLPFAKMIRVGMLPELKSSKTKRGDVVDPTLSGQLVSAVKNTGEKRKGQ RTRPKSKYQV Q57VQ9 ms34 Trypanosoma brucei brucei 927/4 GUTat10.1 0.022421469 1 Trypanosoma brucei brucei LEVO MLKRTLTVLDQTYGPHKSYKYTYMPDPRKLAPIETTQRSEIVPQSIRPPTSYVPNHETFLERADIHRLRPTSDFKASFKD WNDLFTCDKRQLRVRGIPRMTRDAIRTAVQAFQNGNPPERFDTKEEWLYYKQFKTVDYSYRVIPELPEKYRPHQSGIDQA PLPDYREINKMPQWAECEERRQSKKTV Q389L3 ms42 Trypanosoma brucei brucei 0.029748666 1 Trypanosoma brucei brucei LEVO MRLCRRLSYHAYVPKGASVQMGETFMPSNIATAVFRETEAAAARADKTKEETGFFSQPRLNYPVSSGIPALFSKDQFDMQ YSLFHRDVVETLNRHTLGTPLEGHNLETVIRKTSFDATQAVAHTAASEHFNYCFFYKSLRPWGTAPPPRLREALQLQYGT NGSADAMDEVRRIVWVTAASHQERCGWVYLVWSGVHFDVVEFPHGSCPIASDLIPLLALNVHESAYILDYGTSGLKQYVE NYFKACNWPLAERYYLMAIGQVV A0A1G4I0W4 1.15.1.1 mS43 Trypanosoma brucei brucei 927/4 GUTat10.1 0.04859041

residues 27-35 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLRRTVTSLRNARELHRLLPALLPLLDPPVPTGASVCAAHLIVATGHVSKQREPHMSNCPVLERKIPLSEIASDDNFLKH PCVPCLKHRINFLASEDFCYDFVERCGCGTDGALPPEWRCIVERQFGSMAALVEELVIHASNRREAGWTWLVYDAQQAGE RPLLVVNMPAQRSPLLLGLWPLAVVNVTEAVLVEAVRAESSGSVAAAPPPPWCRAARASLYDTKAAAEAFAMTAPMSEGV GKKLSEIREKVARDAVSRMNWGFILQQLKKAETYYASSDRGEKIRVHRMKLEREAVTKAMSGLRDSGATFLTHDTVEVTT TPSANSEPSSPERVPGVQAKQPQTEGMDPCKNKASAEPDPKCTQLPDGTWQYSYRNGDVTLLKPDGTRVFRKRELTTTAY PDGSMLFQYANNTSILDRADGVRVTTFEDGTTKEEMIRR Q585I1 mS47 Trypanosoma brucei brucei 927/4 GUTat10.1 0.137935 1 Trypanosoma brucei brucei LEVO MERRRLLHDGRWFTPCVLYGSARRGLKTLDVREYRPLAMPIEFRFYQRYANHPNRQSGIQFLTHYNTHQRFRVNKDYIDY MHWGKEQGQARLPHRHQRVAFDFNDQLHPTRIGTPEDGGNANHCYPEDGEDGSYAWFASQDPTFGHHPDLSSSFDPNYRV FSHPEHWNKMFTKRRPGEGDIDLSVLPSRSLLGPLMAHSDTKGIPYFKVDNRGHSNGRVPGVNAPFFGEFDQKMMQAMSR PLNANRTITGNDGRFSKTIMINEPQCNQALSAKTASELSKEIDKATNAVYSKLSVLEAAQSGLTDYFCGGLNFEIVGFEL HMAAMLRERAAAILERSGAGGLPSASSATLSSSSTTRRCAMLLEKADEREVNKLLRDASRYEDRVDDALRQHASLIWRVY TAPRPLMTLTNGKCRGTGCGLSLFAKYCALKDSSEFIFDGPNVGITPYGGMTRLLARPETSLKYPGLAEFVVLTGASLFA GDALRLGWTDLFTTIPDMTYHIKEWFDTTEHMHNDAVAWQLGHLLETCFKMKESHSSAMERAAITPTRARWVEDAFADQP SVNDIMRTLTEIEQLPFQSTHNTSDESHITPYTLGSVAAGVQRLGEHRLRYTLSPWDITPPEDSVVLKHTSEMFRAYVLE RRGATDVVTHRDAERAAAWSQQRQREYDAYCSLKEAPHPRHVYARLEGCEGKIVSFEFVFEISCRRSGPQGTDEVLQTTT ISRLKSRILDALGMPRDRNIDLGWYLPTLDTCPIHSDEELMQVLHTDPGIEDPKEKLRYPPIYFIVKRCTLYFSEWAYAV KHQLLLQSPFALRAAFGLLQEVRGDGAAENVMPLAETLGTEFKYFTRLMRRPDFYRVGVHTDKSVEKWEEIKEERRHNIH HTHRPTRPLPDFEEVFERDVEIDGHRFVLRPRWNPRTLQDVDDSDILRLRAPLTYDMDSTAALHVPTHCGKASRLAGMLQ DAGGVQVLDGLGEVDDKGEPKVPPLQSNASVPTNVSFYEMARHPWEDRASSWRRDGFTEGSLEYFESQYRSAERAVYDEE GRGVRNYWPSRDAVEGIAQEEENDAQLLQERLFNTLETAGTSVEPWARNLRLNATTGKFGYKTEIATQEEKIYDDEYYRW FIQPGRHPNPSGLTLNGRKGQFEEGHSRDEELERMWRKVVADGNNDFAATTSGMPVYDEDAKNAEDSYAAGDDVDVVTTD ANVPVDGDDSS Q383R4 9s rRNA Trypanosoma brucei brucei 0.198442391 1 UAAAUUAUGGUCAAUUGUUAGUAUUCAUAUUAAUUUUUUUAAAUGUUUUAUCAUUUUAUAAAGGUUUAUUUUUGAAAGAU UUUUUGUAUAAAAUUUUAGGAAUAGUUAAUAAUAAUUUAUAAUUUUGAUUAGAUUGUUUUGUUAAUGCUAUUAGAUGGGU GUGGAAAAAUAAAAAAAAUAAUUAAUAUAUAUCAAUAAUAAAUUAAAUUAAUCUAUUAGUCAGAAAUGGAUGCCAGCCGU UGCGGUAAUUUCUAUGCUUUUAAAUAUUAUACAAUUAUCAUAUUAAAUUGUUAAGUGUUGAUUUAACCAAUAAAAAUAUA AAUAAUUUUUAUUUGUUUUUAAACACCAUUAGGUAUAUGCAAAUAUAAAAUUAUAGUAAUUAUAAAUUAUAUUAUAUUAU AUUUAUUCAUAUAAUUAAUAGGAUAAUAUUUGUAGUUUUUGAUACCAUGAUAAGGAUUAUAAAUUGAAAGUGUUAAUAUC AUAAUCAAAAUUUAUUAUUUAUAUUAAAUAUGUAUGUGUAGAUAAAAUAAGAAAUUAAAAAGGUAUUGUUGCCCACCAAU UUUUAUAAUAAAAAUAACGUGCAGUAAUUAAUAUAUUUAUAAAAAUAUAUUUUUUUUUUUU M94286.1 UNK Trypanosoma brucei brucei Lister 427 0.00044353899999999996

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.000613749

residues built as UNK

2 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.00274137

residues built as UNK

3 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.0046136779999999995

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) bL27m Trypanosoma brucei brucei 0.021793092 1 Trypanosoma brucei brucei LEVO MLKCSLCYLASKVTAGNAKNQAGHPRRKAKLFHVVPGTPVTPFEKLKEQRRRFGQDRHSRLPEYRPGNNVRMDPNTYTLY ATKKGVMTIRESRINPKYKWLDVEPDIQKVYRSRELRRALQEREMASMAVGENSNYRVELDLLLEPDWRERVMHVPKATE RFKDPNLFTRGVVNELSPLDRYSYT A0A1G4I8T3 bL28m Trypanosoma brucei brucei 927/4 GUTat10.1 0.028051372999999998 1 Trypanosoma brucei brucei LEVO MLRNTDVRLFSTFALNPETSVAPHGPPRGLVNRYVSMGLPPWAAWCNKVNRYSLYRMSGVTQRSFLPKPPQEMDVIWLNE RVRERVRTSRQVQNVYRQLKYPYVKTGIHYSDVLDHWVQVPMVEAAMFEVEKDGGFDNFILKRSGPELRSTYGERIRRHI LVRQKEIQKNFVLQKQAQMLVESMEKEILPMEDGKKVEEVLEKYGIDKEQLLRDIARAAVAKKQQLASAVPQPSHVEVST N Q586A2 uL29m Trypanosoma brucei brucei 927/4 GUTat10.1 0.054045367 1 Trypanosoma brucei brucei LEVO MQRNLTKLSAAAFYEFVDNNFLNNKRPPVPGGSWTVEVLRNKSLADLQHIWFLLLKERNMLKSMKEHYLRHQEELGAMPA PSRLKMIDESMRNIKRVVKERDEEATARAVEIFKERLKRGIYRYPPGPPPPPGAHDKTSVVKVELSCYVEEERLRELFGR YDVFEPHKGIVRVELKLPDEVLKQKEEAEQLWTQYMAECSDVKAYHQWSTAAPSAYDYTEVELAPGIFANDAISDKGGKH SGDTETHEGVIVAARVPVPPPKEKQPPPKNPLERLKAERRSYLARTTIQLGYFPNVTLPPPRYETVEAVPRPVHPDEIEG PWEAYITYDREDGLSYAQSLGITTIGVATVLGLTEHVREPQPYAVVDPVYCEALRRERAREETLMKWPHVPEWKYEYSTY TRKHLADIVQYNYTNVVDYVDREVLLTGKSVWECPIHIDHTCGGSKTVPPHAKKPVRYMDAGIANVGVTDI Q38EM7 uL30m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.025204334 1 Trypanosoma brucei brucei LEVO MMRRMWVPTFSAQQQHLHHAMVLKMSTYASTPSGNGVTSAASNEEMNLHDAAAIAPGPYRRVGNIFIVHCDDHPFKHSWE VNRMLRELRLEFKGQTTIVPDIPQVRKRIWRVRHIVKVDVLDLDEAKALIGVPEHISFTDLASQLPPSFGRVKAVPSPVI RSKMNFMKLRRMRLRDVLHRDALELRLLELKRSAMKNAEQQKHAQKDERTDNKEGEKC C9ZY77 bL31m Trypanosoma brucei brucei 927/4 GUTat10.1 0.021083229 1 Trypanosoma brucei brucei LEVO MVLHKWAVVSRSAPPPRGLRPIARTIPTHPRLRPVDYKIPYVLRTFIKDRHTSEVQHLENRGMFAEELSIERSRFPRFHS TFTIQTDGSLNEREFEFAVPPIVTLFHDRLSAHRERQLELAKIGKLRKERNWETEQKGEESVSMACNALAFPYCIPKNML KRSRVVDPLNSKSSTQGVTSGGG Q38AB0 bL32m Trypanosoma brucei brucei 927/4 GUTat10.1 0.00974571 1 Trypanosoma brucei brucei LEVO MFRRTFFTPMIAQPTLLMLGNKGGTPKRKKNPMQLRRKVYGLHFKEKYLKMEEWYYCPLCAEPKKPGEWCRREDCRQIKP Q584F4 bL33m Trypanosoma brucei brucei 927/4 GUTat10.1 0.013042739999999999 1 Trypanosoma brucei brucei LEVO MLSSTALLLRGQYKTRLKKRMVGFIPKVIPRKIKNNMVALRSEANTGHMEGYLKTETERLDATGRKVQKVLWDPVLQRHC LFKETKIKGPFMTKSAIAKKVDFPIGGAEKMLKK Q581Q0 bL35m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.021661453 1 Trypanosoma brucei brucei LEVO MGSEESNNICAYKRTISLAKIYIVLLVKTAMLRYSRLCFPKMGCEEITRKARRVQLQPTEYLAQHRMQVWQLRFKEMGPP FSRVWVALGGKMRRRRVGRQVDVKDMRYYWRPIEPQYQRLYMSRLRIRDHSNKLRQPMRLRATNADIGSGSSSIEWERAS NRKYGAMLAPPKRQDFEFRVV D0A1K1 bL36m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.021795226999999997 1 Trypanosoma brucei brucei LEVO MRGTTKPMLFLCSTAVRLNFFPLGLSAGPLNSHILLPRNNFDGRTTHGMKKIQGQSKDPQMVMSRDELKLRCEYCRFEWI HDTLCVRCPAQPSHDQREMWLHSTWMWGKQQPYKYYKYMPAVRNPRTGMPMAREDARSMNNERRGQGLTTRTLNLEKERR GISRDVSRIGQYNTRWKTRFPFPT C9ZMA9 uL3m Trypanosoma brucei brucei 927/4 GUTat10.1 0.055073699 1 Trypanosoma brucei brucei LEVO MRSLIAMRRRFVSGTWTIAVSTQQRHRSGSGPGDKRIRTDWYRCYPSLMREKDRDMYHCYYPYLFDHGDKMSLYPKIPEN PREWQPEQLQTTYDAIREDKYDAFIRLREKFPELYQDTRAWDNPPPFGEFNMFYSVRFGMVGVKAFTCKDYDELGNQFDC TAFWFPDNQVVKHSTRNGEVGTDKVYVGAMNVPVEFHKPHVAAFYKAAGVPVKHVCAGFPITPDAYAPVGTKLDVRHFKP GQEVTITFQNTDYGFRGVMFRHGFDGGYVWLGDSRWQRRPGAMGTEGQKRIYPGHRMAGQTGAAAETYQGVPVWRIDYKN SLIYLPTLLDADVGTYVRFSDTINTKGLTLWNEHRGLPAFPTFIPPEDEDLSKLATDECQLKSPPLYMYFRDEFPATQLV SQADVEDAKSAKPATAPPKKKVYDMKKYYEARKKYRQSMQKARKYKLMGLRTKAHEKQEEARRAKILKYKRMK Q580R4 uL4m Trypanosoma brucei brucei 0.05315666399999999 1 Trypanosoma brucei brucei LEVO MRRVSFSVVLATFGKRHSSIFSPRYDWRTSGVHDIAPRDEGDFLYQGPQHVLPGAHPLPLHHPHNTITRPVISPYIPSPQ RSHPYFTAPLPELPHFSTTKPIVYTYGTMKERIIAPVFNLKNEVIYTRELDPFIFGMYPEVEELSKNLTYWMVRCQNFAS KWDYETREIWRKAKKNWPNTGMGMPRVGNRKNHLYTWGGRTKPSKPWNMLMPTMDVKTWSKSNRMMLTLKMLQGRLQVVD RLTLEEPTQECYLELCRNMSWDVRHTGGGVLFMDGGSRITPSSEFDRAFFFGSFFNGRNKIVRPTVLCDEQYDYNKTAAK QRMKGPKGAKNPIPINRFNAYDAMKHDRLVITEGALMQLEDELYEHKLQILPPHIRNQLPEYGYLDSEALGDCVPSLKTI QMEAAARTEEAESDMYKSFIDNPYNPWKDNMDASYAVDGADGTVQKFVDGKKVSWSMLS A0A1G4HYD1 bL9m Trypanosoma brucei brucei 927/4 GUTat10.1 0.031032696999999998 1 Trypanosoma brucei brucei LEVO MRRTVVQRVAPWVPPPRHDIKVTMPPPPGGEVGGRFGVSQGYSDRLARTPYWKRMALSTYKLRMMENATRYPMSEHRPGE YDIRYLPTPYPCTIRNRPLLEVGEPRQIPSIRIPVIFLVNLFDEAKGCWFGRRYETVYVERQFMREELMPQRYAIYATPE AYKLLGLPVVNHHTHEEIPKTPREYEKLLERQRYDEERWKYTIEYLFRKYEDGPPELLDRPEDGWDGSEEIALSSVAGAG RDGVVSQRKGPVKQRKARKIKLF Q57UC5 uL10m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.020283566

residues 106-129 built as polyalanines

1 Trypanosoma brucei brucei LEVO MSPASPLPVAALSRLRITHRSFLTRSRGRHVCRSAVGVEYRPEQQKKVLDHSYARVINAEVVHGDEQKFWGERRTFYTQR NIFFPMWDRCAQALILITREVPRVPQEMAFRLMAVFLKLMLLPRLMMNTELMLPMWIASNAEGAMAAAKDGSKGKEQSSK QQGESKDDAKKEGDNTK C9ZPD1 uL11m Trypanosoma brucei brucei 927/4 GUTat10.1 0.04025275 1 Trypanosoma brucei brucei LEVO MLRACGVLQLRARPKTVCVEPGSNRLPEALVVEKARDIFGRPEFPGKRVLHNWRFFIKAGKAATGPPVGQEFSKLGLKAM DFAKVFNDRTKPHFKEDVELIVRIQVYFDKSYLFTIEPPPTAWFILRALRKKRRETGPVPLRGHYCALMTLEMAYEIAKM KPLCWGRPEYPLLETRVRRVVGQARRMGVCFIGVDTPYSSPVKDMTEQQYTEECERYRRIHMEQYTTLRQRELEEAPLIE RLHRPNMSPLTDEQIEEGLRDPCLLDTLWRASHPLSPYHRDLRERELARRYLNARGWVKDMTPEEMRIVFMNYRLPEGEK RKQMDEAAMSGEVYWTRDGAQL Q586R9 50S ribosomal protein L13, putative Trypanosoma brucei brucei 927/4 GUTat10.1 0.024341309 1 Trypanosoma brucei brucei LEVO MQRSTRIVMAPFKSVLRRHKHHPEGLPLYNDMSKQWLCREGERWWLLDARGEQLPRVAAVAAQYITGQHRPDFTPGMITG DHVVIVNIKDVVMVGDQWIRVPITWQTAYPGGKYRIRLTDMYERDPCMLMWWFLKDEVNRHFVRKLKTRIAPLEKAWLYE DSIHPHADKNPRPLCWTDPEVKGWKYRDPQFIRRWKPNEFMH Q580D5 uL15m Trypanosoma brucei brucei 927/4 GUTat10.1 0.043780016 1 Trypanosoma brucei brucei LEVO MLSVSPPLRRYRLFHPRREAIPMHMCPAKTIFPLINSNNLLVKTRNSWEDFTGRKEFDEDHPLPVVGSRLNGRTTQHKWN HWDQYLNPQITQSIKDLTPTPEYVGMRCGHNMIKMGWMKIGGSWKYSRGYNDRRRVFARGQWQERKMTPRFMLAPRVSAG GPRNRYEGKLVFSPLRLSKLLWAIDTGRINPNEVITLYHLRQANVVGEREIVWPGFVLISNGVRRVPYPIHIELQNASAE SIRLIEEAGGSFTCVYMTHEGLYQELHPEEYPIFMDQELPERRGLESLATNPSKRGWLTRWYEDSSKYAHPAAGRRYSHY LKPPTERDFPATVEEYEMVKHHQKWHLNQPGTGTLLPWHSYNTADLVKRAAGRL Q57U68 50S ribosomal protein L16, putative Trypanosoma brucei brucei 927/4 GUTat10.1 0.019158144999999998 1 Trypanosoma brucei brucei LEVO MPSPFQQTLLARAYHYVHPKTGRPIRQFRRYGDPRYINNEAALIRGQWGLITADFGMVTQSQMENARLAILRRLPRGSFT LTMHTDYEEFPVVKKSPESRMGAGKANIHHFAFKFTTGVPLFEIMPISARRLNQAEAEGIFLAGRPFIPLQTVVVPQGRV DEYHVFK Q57UM6 50S ribosomal protein L17, putative Trypanosoma brucei brucei 927/4 GUTat10.1 0.035117824 1 Trypanosoma brucei brucei LEVO MLRITLLVVGRAPPVIGCTRKARYAGIDDNPTITYKPWDTTEPLMADYGWTRGKLPKFRARSPFHRQQIARRMVTEMIRK DYCIVGGARAPALRILADHVVELAKAGDTDSRQQLAYFLHDPLMVDKAFDEYPRRFKDMNAKYAMMTRLKSRRRTDAVAM YFVEYKNRDMSDNHKGEDYSAGPERFFLPPRIVETEKGIQRPPHMQMAFDRWASKFKTEEFHHWWRLRHAKLRYWGVRNV PHPSDVDPLWTEKEEEEWHNEMLANTGEYEDLDLDDESYTAAGEGGEPSPAGDGPQPQRVG Q57YI7 bL19m Trypanosoma brucei brucei 927/4 GUTat10.1 0.016931455 1 Trypanosoma brucei brucei LEVO MGYTRERTNRHFFVARANAFFSRLPIARVQRSLAMEAVREGRMRPWKYTKEQILGAPVTCNFEYNPRPVRLIGTVMDAHT EETSIKGGLKVYARNEETNMMLWIPPGNPKLRHEVTSTKGSFQHYLDERDKWDEAWITGRARMK Q4GZ98 bL20m Trypanosoma brucei brucei 927/4 GUTat10.1 0.024393942999999998 1 Trypanosoma brucei brucei LEVO MLRRTVCVQHYRAKLELDRIRSMLRGRARLERKVGLKRLFFLMRTQTRYRVEQQAHWERAIVRKNVDSAAREHGTGWQHL RNELGRQNVMLLPRSQQLLAQYEPLAFRAVVELCASRIPPPPPPVVASVPEESYTLWPPASHDNSECASTDGSDAPHGQQ QSLSHPAARVELRCGVERVLRRGPSGLGNNVNELIDAWKEFDVSPLRKGEVNK Q383R2 bL21m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.020895267999999998 1 Trypanosoma brucei brucei LEVO MPSLANGLRCPDALITRTLNPLLNGPKFAVIYCGNHQYKVAVGDVIAVQRLRVEIGSHIALKKVLMVGGPRFTAIGRPLL EGVRVTAQVEEQKRMRNVVSLFATPGRRHVRWVDAPHAATIIRICEIFYSPNVVGELDKYKGELLETFTPGQHTNPVYPV DDGYDVFRKKDKEAMENATTFLDLLGGV C9ZT11 uL22m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.032083284999999996 1 Trypanosoma brucei brucei LEVO MPRPAPRFAMGHMLPHRSNVGSQFLHTQRHGSRSTWYKKHYFSLRPFAIQRHHGTTPRILLDRSLWKSLWITKLQLPDIN RWERVVNSRRVTEDRYAFVEEEGVMHKVNWGLYCERLETELTVTQERLPQHTLLMKAVPSSWKKLDIDISVIRGLSLREA MAQCKLSLRKGHQIVFRALEMAQQGAEAKGLDKEHLRVAHISCYPGPTDKQIDIRSKGYYAWKTKKSSHLVLTLAEDPEM VLPDRTCLPYSSLMSMKRAGLSAQPTVIDVPAITADGI C9ZSI8 uL23m Trypanosoma brucei brucei 927/4 GUTat10.1 0.028822936 1 Trypanosoma brucei brucei LEVO MVVSLRRFHSYANSSRGGATTCLITGGGKGHFALTVSTRGRFYRPLVDDGINLWRRRMGRIHKGWRTWEYQHTRPDPRPF PDPPVNDYFGRSRIWNPIACKLGYVRKKADEWGWPNKPPPPTGLRHSQEFFPFFFERYFPDAEVRLLLDSVLNNETKRPV FQIPCSMSKVELVNYLKNIYGIDNVVRVEVRNRRGRRYKNEVGEIKMMDDYKVAVVELDTPVSVELKQIKGTEDTSDNRP QERITQ Q387G3 uL24m Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.043999047

residues 313-340 built as polyalanines

1 Trypanosoma brucei brucei LEVO MYARFRTRSKFYFRPARPALAYNVDPNVMRRPKVKRGLLKGTYSDETVDLRDRERLELLESMRHPRERDFYQDHTYHNQW LRRDLEKHQKQQLAARYKYFAPDFEISPWIWYPGDIVEVVSGEGIGQRGTIIAVIKYKNEIVVQNINVQDVVIPASESRP EQIVQREHPISVTRVRHVDPSTNEICNIEMVKVRNKETGEMEEKRMSLESGILMSIPPVNDELEVGDPLKDTPIQDADEA TYDREAEQAVLVDKRLEAMEEHFVQSLKQSYEFHEPLRRKNAEDMRQFQTDVIDMACAMLGERLLDTVNASDTSSFPAEW QEAIAMHVEEIEAEMEEVAAKEMAEAKEGGAAENEQHDDDLDDDEDELDTSEESVKHV C9ZK52 mL38 Trypanosoma brucei brucei 927/4 GUTat10.1 0.058437895

residues 41-53 and 294-311 built as polyalanines

1 Trypanosoma brucei brucei LEVO MVMRRCSYNVHPTWYMMPLRAYHMPGWKTKVSTKDVRTREQREADRKVRQIEKLPRPQALHDFQYPYEKTVLSDTMFQYP VYENELDTPHLFNITEPPDFFVYWNVSDFERTSYPPLPEEDHRLLLPLSGSAPWKQHRKRSLKYLRKHEILPNYLPHVRQ DVNLSVVFPGVYATRARLCEETGEPLPPPPPVSRMTHRNFWMTAHCGNYIELADLQHPPSIFFLDTVSATGQGKAVEKDA TEEGSDEVWYTLIIASPDYPFRVPASVDASTQRGFFLNYMMSNLKGGGNSTVLEEYESEQRQNNQKRQHLEELVRNPAPI AKEGDVIVSYTPPLPTEDAGTTRHICMLFKQRSYVSGASCALDDSKASFAARANFRLHAQHRDGIPSSSVEMLSRIEQVL PPDPSAVTFFQTKWDIQVQEFYESRGMLEPAAPLDEEIEAILAYHARKPSELRVRARHRPDGSTNVGDDPNFWAQAEPTR MMDGSMLSLWSRRTTLGANGVPITYRR Q381T7 mL40 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.033646065999999995 1 Trypanosoma brucei brucei LEVO MVLPRKLVGQCCLLLQKKQTAASYMANAGKVGNEEKWAQAAMEYLHEKRHCNDSRKRQHDVDNERRMAFAFDRYCSVNEK IFTERLSRLSDRMTEALETIKQLGMDHALEEALMLSSEQPPLNFRRPTLTPPVAGYEPGFGLDVPQLRSRQAEYPPVGRP TDAMEFGEEKDPSFPLVESFRVEDLTTQCLNELEERHGEIREAAPTTGVEGEAWEAYVALQKKALARQQLIFELCNNGEL RERYDSDVAFRQRVWEERGMLPLEIERERLHEEPRHYAQEPAYHPFRKM D0A511 mL41 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.022556877 1 Trypanosoma brucei brucei LEVO MLRCSCACRRGVYHNAPSVYPFVKPFHDTPYDQDRGRHDSVGQRYRKNSWPEWMDNGADGTGYGIGLHRTHPLSRLRGNL KRSPSHVPRVLGMMIQGVWHKSGVKLYFRGGKPPNPSVHPYLTGEPCPLYGWKVTDESVIRQFNMPSIDGTNFRYKPYVA LQERKIMGVQVPSDSVSLASGRTTESKPLAKRLFFWR D0A8I6 mL42 Trypanosoma brucei brucei 927/4 GUTat10.1 0.021602639 1 Trypanosoma brucei brucei LEVO MLRLCRVSLRVQSHQKKRAQHPNAGTRFGRVYNRGFIRYGFGGFGMSVYTPKKDRRFRVQPLPSLHANSLADDTPLVTTT RTLSPNFRAFALQDGGVFFTHPSHEQVMRVGQNILAEETKATGMTSMDTYVNSRIQSIIAENTVENVALSHWRRRHMWNL VRTHGKLQRHWGVSDATKGARSNIYGRPS Q383S1 mL43 Trypanosoma brucei brucei 927/4 GUTat10.1 0.031300405999999996 1 Trypanosoma brucei brucei LEVO MSRNGELCLKKVIISYCPSNGAPNTRQFLATHLPHFHAKYPSVSIDLRPRLWPEMAITGVYRDGSERSYNTKNLSPMGIF LRLNNLVSTANDYDQPFCASHLHFQRRSVQGTWNPWLWNYETERRRTEAPQWRRKLSEKEWDYYVGQYSAQMKQEEDEIQ RRVADRTCVQEQSTREVQERWKRHVVPRMQTDLEFNLSHFKRQHARGQLQQRPVTMGEYRLFSVPDPRELGQDAVDTMRR RESHNMEVWWRKRKEQLKPP Q583E5 mL46 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.033984199

residues 149-159 built as polyalanines

1 Trypanosoma brucei brucei LEVO MRPVARLPLSRVLSGCRGFHSQHRTPVLPQVPAAEVQQAASESKVRVAYLLHRQPVVKPTPHPLEMEMAFLLQREHQRYS RHESSESATHFMAQRGQSIDALNRTDPRQIQSNFFGLELYQDAMRVVLQRYKPERRVTPRDLWDPATYGSSNANSNASSP PTRHSLHRKLDDYLHLIVRDEASGKWTVPQTELRGRETLRMAAERAIATDNGEGLDCYVWSNAPQATVPNANDGSWLFIY VATYLSGRPKFSEFRPKTIDHAWVTRHEMMQYEENFQSPELVRVLLDISADSTFES C9ZT91 mL49 Trypanosoma brucei brucei 927/4 GUTat10.1 0.024501486

residues 102-109 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLNRSRLRRVWLGGTAANTSLGRDVMPSTGKYINIVSPTDVAANISAGAMPPEANVAAALPVPRIQYSKWNRALRSDVYD ELLKLPLRYKLHDFAKLCPPQRCQEEPEAVGVVGRDSAVGYLPPLGPADPLDTIPFFVHRSSNGVLPGKVFSMHPRNLMP AFYLRIQQVEGDMFRFEEELLKIFPTKKIFVRSHSIYVYNVALDGRMVLHHWLLGLGF Q57Z82 mL52 Trypanosoma brucei brucei 927/4 GUTat10.1 0.029175984 1 Trypanosoma brucei brucei LEVO MRLHTVRAPIITRAAMRGYSEARSNYDGTSLPAWPAPGKKPTYPAALSELRLPQPRMRKTRTEWMYYHGHGGCPGKYGPS REIADFEYADGTPASISGRRFAFKHHQDHLLVQLIRAAATVERYDASGLLPRIPGTAEQRNWDPAIPLFLDDVDEQGRPA PLRTAGDAPGTMVSHVCSRVVDERMGTPTHTPNELANRHEGETLEANTMFATNDPSAFVSDTVKLRDDKRPYWSRRRWAL TDKFLVPKSPKPKNTIKDE Q385V2 mL53 Trypanosoma brucei brucei 927/4 GUTat10.1 0.035656383 1 Trypanosoma brucei brucei LEVO MLNPPKHYSVESLRTVGLLPAQLALSRKPRLRPHVGNLKGLVYPLPYYAMWRGNHNKYTYNKSTVCLWGEGDTRSMYHQH YAHAKCPTDYGRGGREFEYLTVKRGKMLQKPLPRVQYVAEGSKPVWLFKSWHTPLSSPSMWEREVQYAEHTPEHIGAKRP LAVVAPRTMHRYLFLMHMEKVTITVSPLLFGYGHTIQKAVLDFYRRAISARSPFPKDKVFLFYAIDHITPRIEVTWLDGT SYVPPVLEGASSQDLIQMVMEEAWLAADRMAAEGRVLNPLAIDDYKWDQLVVFKKVRDKEASKGGGRKK Q57YA9 mL63 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.018213924

residues 64-73 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLRHCTAHRRYRTAWRELLHPLPVRARKMEWLKRDAVEENEEILRRPYYTIKSYALPPAVGRQESIHNSNNIRGGMHSSH SLDLIMRQPRRVKTPEQLRALRDRLRFIGVTGPMPQATSVSTKSYTDTYGSRLRPRYPESWDTVPPHQPSRELL C9ZU82 mL68 Trypanosoma brucei brucei 927/4 GUTat10.1 0.028545976999999997 1 Trypanosoma brucei brucei LEVO MLRGTRGFLAVSPGVGIAPETTPVKYTPMMLNIQNMMWWNGKRNLYRATYREKTWYEISRTGAFTKGRRPVMRQKYSREA LQAALAMVPPGFEVADVPRPPQRILAQSEGIVGRWYSNYWTLHSMRYQCLLAGVEWPLGERQRPRTNYDEPFFFADFEES KAIRDYRSRWINVNRSLVGMTKRMKEAEEEARYMQFRKLQDTFWSNRKVLVNRVKSMYNQGARTSAKDMPIKTINIKAFL SE Q383B7 bL12m Trypanosoma brucei brucei Lister 427 0.003998369999999999

residues built as UNK

1 Trypanosoma brucei brucei LEVO AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA bL12m Trypanosoma brucei brucei Lister 427 0.002008194

residues built as UNK

2 Trypanosoma brucei brucei LEVO AAAAAAAAAAAAAAAAAAAAAAAAAAAA bL12m Trypanosoma brucei brucei Lister 427 0.0019371159999999998

residues built as UNK

1 Trypanosoma brucei brucei LEVO AAAAAAAAAAAAAAAAAAAAAAAAAAA 12S rRNA Trypanosoma brucei brucei 0.37701562499999997 1 AUUUUACCAAUUAAGAAGAAUAUUAUAAUAAUGGGUGUCUUAUAUUUUAAAUAAAUAUUUAAAUUCCGUGUAGUAAAUUU AUUAUUUGUAUUAUUUAUAUAAUAGGUGUAUUAUAUUUAAAUUUUAAAUUUGUUGUUUUAUAUUUAGAUACAUAUUUAUA GAUUAAUAUAUUUAAAUAAUAUUUUAAAAUUUAUUGAACUGUAAUUAUUAGUUUAAUAUUUUUAGUUUGAUGUUGAAAUA UUUAAUUAAAGAUGUUACAGUUGUUCUAUAUGUACCAAAUAAAUAUAGUAAGAUUAUUUUAGUUGAAUUAAUAAAUAAAU AUUUAUUUUUCUUUGUAAAUAUUAUGAACAAUUUAAAAAUUAAUCUGUUUAACUAAAAUGUUAUAUAUAAUAAUCUAAGU UAAUUUGAAUAUUAAAAGUACAAGUAUAAUUUGUAAUUCUAAAGUAUAUUAAUUUUAUAUUUUUAGUAGGUAAAUGAAAA GUAUAAAUGGAUAUAACUUAAUAUUUAAUAUUUGUUUAAUGAAAAGUAUUUUAUUAUUAUAUUGUAUAGUAUUAUUAUAG UGUAUAGUUUUUUAAAAAUAUAAAAAUAUUGUUAAUAAAAUUAUCGUAUUUUAAGUGCGUUAAUUAAAUGCGUUUAUCUA AGAUAAUUAUUUAAGAUUAUUCUUGUAAAUAUAUUUAAAUAUUAAUAAUUCUUAAAAUAAAGAAACAUCCUCAAUUGCAA UAUUAUUGUAGCAUAGUAAUUUCUUAACUAAGUAUUUAAUUUUUCCAUAGAAAAUUUUUAAAUUACAAGAAAGAAAAUAA AGUAUGAAUUAAUAUCAAAAUUUUAAUAAAAAUUAAAAAAUUAAAAUAGGGCAAGUCCUACUCUCCUUUACAAAGAGAAA CAUUAUGAUAUGUAAUUGUAUGUUUGAUUGGGGCAAUACUAUAUUUAUUUAUAUAGCAUAAGAACUAUAUUCUUUGAAAU UAUAAAAGGUUCGAGCAGGUUAACAAGCAUUAAAAAUAAAUGUGUUUCAUCGUCUACUUAUUACCAUGAUUGAUUGUUCA UCAAAAUAGUAAUUCGUUAGUUGGGUUAAAAUCGUUGUAAAGCAGAUUUGUUUAUAUAUUUAAUUUUUAUAAUUAAUAAU AAUUAAUAUAAGUACGCAAGGAUUGAUUAUUGAAAAAAGAAAGAAGAAUAUAAUUUAUA M94286.1 mL67 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.093569273 1 Trypanosoma brucei brucei LEVO MLRRFALTSSVALRLRFERDSGHNTVRYRPIPESMQPKHLEDNFTPFPLPKFDESLEYGPVRLRNIPDIEAAKERRRGSR LAATEVLLQETLQEENQFATSGKGDGNMAIAITERHTEDVTTPAADSRFPSQTMSPCSHEEEMRGYVVSRDYPLIDRLHC TRSIEELVAQFEDRPQIESRVAALADMASTVSFRSDEELLRMFTAISAPFSVDGRGLNFLTVKVSKFGRPYYVPNSLLPA YVNLVDATTIALVREQPWRLSASPALFIQVLQFMALIKVFEPNKWFTFSDHAPSNRADYRHAIGVNHSTAFWGTGEELYD FMVELLRVEDDGRIPTMLDLCTREQMVDLLSGFCGVMPCGKAVGDVFKTITDAFLRRVRNDISGPWSAHDWAIVERMYLV TVLCDAGNNEILQLLLSDTASPRGPDFFAAVSRTKDTPTKKRALCLLQEAIDNASAKADKVTLLGLLESGSEFLLSLVDK GVAHTFATQNLFDYRILNSFLHCSLVADRLRVEQSVITSLIPSSLRDVQVQMLMSNERNALNPLTSSLPGNSGAIATAPK LKRPLMTMLSQLEYLNSIDSVFILHSSLMATSTDQLVSAVRRLPSGKDSLIVTMSCLRALSVKSLTSPSMKERIACARAL EIVSYELEKGRAVLLPFSEEILLHDAGAYCDEDLMLWTVAAFLARELPLVKVHTLMHSNCTARTPYRFLKGGHNLLVSSR SLYDKGAPLLSSLHSKELRLVTHNVRLRTPVRDRKCTLQYYNPIRARFVYRRDKPLFDKYHVTARNLAPGFSRGALKHDW RALGVYTPDHPQVPYHPLQTWMLGETTVEAE D0A5V6 mL68 Trypanosoma brucei brucei 927/4 GUTat10.1 0.060896355

residues 264-343 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLYTRRLMTTGGSATADGAVSYSKGSYHIVPKKYTVGKRIAVRSYLDRNRTELSDRTYMPQKAWFEPYTPKKFDMEHQRI SHNFYNLETKLIWTAFDTPELIGILLHDETIKGAPHLYDAEFLESAVHWTRESRYWRCIGITKPFYNKTTLRAQCWHDRG LQVGTLVFSQAMRDALMDLERAVRRKELGLEPNYVWDRWGPVGFIDGARTDHLPRFAHNPYVDPDGVEVTEVDIAPFNTH EQIKERYGAFIDPDLRPFEGVFRSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS SSSSSSSSSSSSSSSSSSSSSSSCDEKVEALRLMENTRHDAARVRTFYEEKCGFSDFMRTPDKVITAAVLCYLQELQRIC TETDWGKPLARCLTDLERVNVMGKDAFLVYRHIEDAILDKKRRVWATRFAGEANEESTLDYLLENFGRRTEQTRNVGTTG TEFDREQEPIGRQVQRRVLDSDKASKLAEVRQKRGKMWSNKKSVFDSLHQKQLQNVTYGVH Q38CI0 mL69 Trypanosoma brucei brucei 927/4 GUTat10.1 0.059010855 1 Trypanosoma brucei brucei LEVO MLRRLAGCVAFRKNVVRPFMNSTALLSSPPAAVSAPVIPKSGNEGKDYSVASGSVDTPVLDQLVGYYVNIQSRKMYYKDF LRIEHQAVRWAFADIRKNSEALAHGLLQTGLRPGQRVLAIQPCNCETFVLQLACAKIGALLAVVPHHNISADKLRFYLNE FQPHHLIAREWIVVPEVKQGVLVERNMHFWDMIYNVIPELGLSYPGQRFQWVHSQEFFFLKKVVITDHNMNLLGVTPMRK MLVWGPFSYYEQRLRRLSALLHPDDPILALEDPHPALEDKLHITYSHRNCINAGFLFGNLMGLKAESRFGVMPNHHVDPV GAIVAPYAALTTGAVLVHIHSDMFTDDHAINGIEKLCVEEVVGMLGKKSDFDLLLRHAGNFDADQYEHLKWVVLFEDASE PTVGDEYLQKLAKELGLEDVFVFRGPLESAYMVMWRSLKRARWEMVPHVEAKVVGDRGTADARILAANTRGNLKLKGPHI SAGYYNNAGLLTELVDERGFCSTSREAVMDERGQLTLHGTQLY Q584V5 mL70 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.062442410000000004

L1-stalk protein residues built as polyalanines

1 Trypanosoma brucei brucei LEVO MCLKRKAPHLFCFCLWSIFLSFRCFCFRSYAIMLPLLSFPTIQISIFLSFKLPITTFLLSPCFVFVFVFAIRYCGELTLN AQLVLFLLYHCAQTQRGPLKEGEMPICPGLCGELAAVPFRVFLGTLPTLAVEERFLRQLQPVFAWYSSRKRVKEQANEFI EIDLASCDAELLLRYSHIYYVRRQLFDELIERQMTLLDSGKAPKMAEPSLLQCLAGCNMTIADRLQLEIRQLGAAKRAAS VPGRRELDPVARLEVYDYACMMRLVEEDAGAVGDAEMKARAYLPREVIESKLGHLTQLLLGSDARAALDKKDVKLLNRMI PPDYTRVGCVEKLRPFDVTAYFRFYGERINNVKVENYFKRALWGHVYRRFATTPSFLSGVSTYWARHSGLDASFTTTTMP QEVAVAVCDQQIQFPAIKFRAQYVYTSPETARQLWRTDAAVPLMRLFPLMGSRTAEDLAAGVLTDAFWMHLGLSEEENLL QDSLLLKVRRFVDEVGDMYETNIDSVLKRVDDNFKQVVPQLKAEDLQVDAPLQDGEGEDTVRETVAA C9ZR91 mL71 Trypanosoma brucei brucei 927/4 GUTat10.1 0.05031379699999999 1 Trypanosoma brucei brucei LEVO MLFRSVSCKNYQRGGWSPGSKHQKHMTLNPTLYLYRFPGPHGPGPYTMKYWWTLGCFPTGMEVPFRLHEFLSTYQQEHVP VEVEEWLRCYIKDPLSELVNASNDFFKAVEVYPEVESARGYKTLQPSIAPLLVPMKKFEEQLGVKISPVGLRSVLSNPVL KDRFLDDLFDYKSYVEKGGSTPHRRLARSRFEGSLSVLGECEKCLPEQHQVEISESLGTFIGATVSPAETTADDERSLIL LLTTISEGCINAGNYSDAASVLADALMFCHDPDSQATTHANISFASLLNADFKGAEYNGREAALLQPQVKPTSTACARGY VGWAAAAAYQDDFEKAEAIVKDGLTLYVGNEHLEKLANKLQALREEQPSVYKQVPRSLRESRSHLPSQQSRGLLSGSGKG FSNEFDWVEFKNKLYPSKMDPRNNEMGSVFRRVGDLGSFISTSRSMERL Q57WG1 mL72 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.049407 1 Trypanosoma brucei brucei LEVO MSRSAFSSRGALLFSSAPLQCQRRWVTRLYTSYYTGVLYPNQLVQPKQRLPADVSVSAILQKRSEPEAAAERGSNKGDKV DVSKGEKTPRVSWDDADSTDVMRVDNTQQLKEAPQVERPYVPLGEVAKLELQGDYYMEGGMFQEALEHYGVVAKAYNYAY PENHAQRIGIRIKLSAAFRQTGRLESSLANIEEVLRMLDASTRPSLELICEALLELGITREALGMKREATEAYEEALEVV NSFHNWGESHRMLRLLPRLGRRFNYNFEEKFVYFSPFDYDRTFALVDQCLERAETIFNEIGDVEGAIRVLQQRKEMIDKK FFNMRDFAGRIHTMRGHWKRRAQHLTNAPTPDELLRYSPTIHQVHRDFKYELTAPIGREKEVMPGVNRLVLDMGNPYRRR GRLSNKMLKDADHKFANYVRQKEYNE C9ZR63 mL73 Trypanosoma brucei brucei 927/4 GUTat10.1 0.041857874999999996 1 Trypanosoma brucei brucei LEVO MRLARTLHHVASATTGGGQMLEGLVNDGPEVAHKQHASFTPFSIQPWQARCVGASRRKLLPQMLMYHGARLGPRPLIILD HSTKGEAGVAEAARKYESILSQLSWDYGAVYIPLHAQCTDSSKDLLEQSCQRICAVMDALDVRWTHFLTYSYGALVAVRM ASSQEFPHRVGTLMSLDTPLVTREFLRNMEQREDIAKAERDINVPEDGLAFAKQALLSSLEGPLPCPAAEDESLYRDYLF DPNRIFGAGGLVRDESRYVPLKSLLGVRHPVQLIVPSANPLSDAAAHSEVFGHRRPAVVKCCQRHEDLFKESAAKEVAGV LGAWMRRFEPDCFISKRYEQAANEMGQLMLSTAQVSSESAGKGGGEPRKKKEKKKSKA Q57Y49 mL74 Trypanosoma brucei brucei 927/4 GUTat10.1 0.039765176 1 Trypanosoma brucei brucei LEVO MLVPGLSLTRRAVTSSCCRPLHVVRGFSTTCTLFGLEQLQDVPTSTSRRPTGLHRGPGKRQTSEREAAQYKFIRRWELQM RDEWDQLEPFKGLPKPKRQFGNEAAEVIWPYALLLERVVKVHPFTKSIYVYYAQRQSTARGKLAAEIARSFAREFLIPIT FHNSQVYTEAEMLLEYSETPWVVLHSLDNGQKPRILPVAPVEGTPAHTAVEQLLAEVVQGCEALGASVADPVTATRVLNE RPLQNQYVRVDYQWFGDTPDERASHLVRWEFEPEQIEPKIRHRTRHVLDWLNYDGNLPTHRAVHVNAMREKARQKAPRTV AGPRTFYNSAGSRANARSSRFGGQAAVGK Q38AM5 mL75 Trypanosoma brucei brucei 927/4 GUTat10.1 0.039841616999999996 1 Trypanosoma brucei brucei LEVO MLRRSDLLLKKGWTHNPGRTRRGGKNLAWRPKMSERTLEQFVPLHLAFPRRHPNSWQERQFHLLGYVKWPKEIGFYNAGD NFELTPQAAYRIYKQNCDETFWTRLHNEKTIIHLLPLVEQDPGTNMVLVDDIFRHHLKRFGADHYIYNAVMQAAAFAKDF PRCEQLLAEMRGLGLEPNAQSYVNMMLGARLTGKPRDQAEAFFREGIKTGAISAVMRLDTEFQMWMNQLERLGSFKAKVG YLSVNEEGASPMPRDMWALWGWHRTEAKFISRKQMISEQVQNRVRSGKELVGTVYQKARRQPWAKYNGMFPYDYNGPARR PAASFVDAPTPTHNAEVCGTAYA Q38CK0 mL76 Trypanosoma brucei brucei 927/4 GUTat10.1 0.039638914

residues 168-176 built as polyalanines

1 Trypanosoma brucei brucei LEVO MLRLSSWNLKSQHHNVLRRSRPHIHKYRELNRWQRQAQGISKWDQSHSHRPLPYVERFNPESVGLTRGTSAFAWKWWHTQ YPWLPNVPPEAAQIDEAQKQERRSHRPPAWDDEFAKVVLNMNDAEIREYLMSKLTDVIFLETQRDGYELRRLDFEGKPLT SLPEPRIIENFVLEEETIRERVIYQVVEGVFRLSPTSADRRELRSVANIIDYVLTHVRAARPTDRERRQERPITSAALAV MQKCPIQPQLGFVHALPHDTRDALLQEWERMHHLDWQFGKAVYTPRSKENVRGNLTWLREDRHYDQRMKFMQEVESGEAR AKHMKLIAEAAGN Q383M2 mL77 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.042731605

residues 248-281 built as polyalanines

1 Trypanosoma brucei brucei LEVO MYVFRFHISSFHSFLVNFLQPSRGVGEPSRLLHHLETHPSYMRRVTSTAVHRTVSSVLSPALLPSFNGTLLLSVCFASCS AAPVRNLYKRLGLDHKATSEEVKAAYRQRALECHPDVVDDNQKAQAEVDFRAVSEAYDVLIDPQKRKEHDKALGLERTVP PAKKQQEGEGVGCDSKGSFNRGVTRPRNRKPFVRGDADRNFREAFHGMSLDQVLFRERLRQRRMQKQMEEKAKSSSSSSS SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSRDPQPPPSDYMPFRPFHGWTVPNGVRTPPEPTLGPTAKV EDVKDVQLAEPAVGDASHQRKLPKHFPVVQASDGSSLLREETIACMERERRLPHNMGKLYSYHRPY C9ZQR6 mL78 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.035104535 1 Trypanosoma brucei brucei LEVO MGASAGLIRRGGGVFPDAVSLTLTPSRRVYGGSGRGDLLYENPDARRHSGRALGVLNGVRHSSQATMPESGQLYYRKLIL HSRPPNGSCAGLQRHCHDTCNWSYLIPSLHRCAESAISAKLWEKMCQLGLEDRSKAWVNLTQYERQRVRDGQNLYRYEVH QRLPLLEESIGWAQLDDLLGWFRSARRAWVRLPTSVTLSRESSEAGVASVVSPSSAMSCRLEGHADSRDTTPGRNQVFDT PERVEQLTEATVHRIREELQRLNRSERSDCEGSAAMRASARRLARDEELSRCVEEELGWHGVALQHRIPVPK D0A4P5 mL79 Trypanosoma brucei brucei 927/4 GUTat10.1 0.033079617 1 Trypanosoma brucei brucei LEVO MRPTFPALGSRAKGYENRVMVYAHRRHRAWYLPPKLAHARSPLANKSPDEYGNTWDPRTGVEWYHRLRRRGAYRHWPWAR WNDDPVRQHQELSCRRTFSAAVTGANEGVPLWNYYAEVGQEYGLPSHFPLSFMAPFIHQYTSRAWSRKEIERHLKVVEER TGLRTIQQACDATSELLEWGEEEMGVVPHGLLQHVVMLAEDIVLQNKKKAYRKAAHERGILRTTTMERYYALPHLRTGPP MPTTLEQPSGEFPWGKFSTMVGGTRIHPLYRPDGFFKDNMYPA Q381N5 mL80 Trypanosoma brucei brucei 927/4 GUTat10.1 0.034252094

residues 145-174 built as polyalanines

1 Trypanosoma brucei brucei LEVO MCCLYTSDVFFWSSLVVSPLPRIVRCVSAPQIRSIPCGSGDFSVMKRSLIARWQSGVHTPHGVVYRGAKMKNWPEQRIPE NFKFTEEQRFRTKAIPRDVGTIPRNFVLGVLYRHQPCEVGGLWEHCTNDPEIVLDSKRHLREVLKQAREEGFVTFERDAI SNEWLCFLTRERYEEVQRIVTAKSEAVDSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSHARRLEEEVANTTRYLRRFQQR EIDYLPYTDLNGKVNFMWWYETRDVQQRADEVLTDSSSSKALAGEEEHKAGSQLEATTASTS Q585A3 mL81 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.029284466999999998 1 Trypanosoma brucei brucei LEVO MKRLFPSAGVSVVLTSSSIVMSCPCNHIFTSRRAYYWPYNEDFVPEGAETSRFQSSGSPGTRRRVLQEYALSPLFGARVP CCVVGTLRTAKEIIVLKRDIQSLLCELMELPQGSVTLGPLQQLREMMLYRHGTPLSPRLGDERQLNMDAYARRPIAARTM MDVFLAEDMSLDEIVNFGRLSLGKLQIALNNLRKENAEKSSADCEDGSAVEPAQLLVDRMGISYCEIPSLDESDYVFAEV GGVAVTDEEAERVAQRWAERCE D0A755 mL82 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.029283675999999998

residues 153-162 built as polyalanines

1 Trypanosoma brucei brucei LEVO MRRCAACLTSLPVSSPTGAAVGAPAAPLKTQAPSNRSLLGYPLRRAAAMEMLYGGICIQHLAQPPFPLRKIQSESLPPPS LQGERDDLELEVKDSTGNVMGYRLFPVNIGIRARTESVRVRSEDCYKRFLAQKHCAAAGVPLQFPAPSSITNSNCLATPR AASHFHPPSSSLSLFTRPADSQGGDVGRTTPADVAAYHPRAWRPYQMLKPMPHNWGPAVRSSGVRGPHMQLLQERIDKKG FGWKRKSRSLWQQDISTAGFRPKRYF D0A0S6 mL83 Trypanosoma brucei brucei 927/4 GUTat10.1 0.025579004 1 Trypanosoma brucei brucei LEVO MLHRTRLAQLINGRILQYFVPYERDEVNPVVFMDVTVEGDALGRVSVELFHDIVPKTTENFRSLCTGERGYSQCPLFYKG IPFHRVIPGFIVQGGDILLKDGRGNVSVFGFPFPDESFEGKAGKHLRGTVAMAHSAPNQNGSQFFFNLARNDHLDGKFVV CGQVIDGWEVVDRVAVLSGSSCGTPVSRAWITECGQSSGDKLEDTERALSGERALHTMPGKEVLDILSPRY Q57WF8 5.2.1.8 mL84 Trypanosoma brucei brucei 927/4 GUTat10.1 0.024773642999999998 1 Trypanosoma brucei brucei LEVO MLRFTRLFREMAPELQLEYMPVLFTRTILGPQGGFAGEERLVKLEVARKYMEAGHAVTPTEELRRGLWCYNPDTDKYDCF IERNEEFLDFAARKRQWLDVYWRVNTGYLLFGRQSWGQGFLINCPLRKRDVAQKLWEQYKVRIDPRLIEFREKDRRTGIQ ELGHNWCWLYLPGAEELGINREVYDNKRVKVRIHVRKMNSMFALY Q586A6 mL85 Trypanosoma brucei brucei 927/4 GUTat10.1 0.01881768 1 Trypanosoma brucei brucei LEVO MRRLGVFCRGQRNSPLLTRGIATGGRVTNEDRRWWLVHLECAPDITPGTFIAWLDCCGTHTCKKLIERNIWTIEQVAALD SDQVDELKYREGCLKMDVVWEHARTIITPLRQREVTGGVESELQGRIMELRKKRELERRREEILKERANVSEQREETLRK Q38FG8 mL86 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.022376190999999997 1 Trypanosoma brucei brucei LEVO MRRCIPARGGFTMKYKKGTGLWDEDHVNDYKTNRYLSARATMRWYQEMERHQTRNSLNARRATQSHNNNRGLHHTGRGAF ERELERRGVQVEKYPLTTTTGTMRVAELVILRRMELEKRAEEALAEQRGELQKKNPTPSEWYDESKGPLNPNFLRSMRSH YEVDIANLPDTPLIRGQREFFIGEERGNGAA C9ZPU8 mL87 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.022337521 1 Trypanosoma brucei brucei LEVO MLNPTFSLYRKTLQSYPVPPKIRHYDRRWSGSRTNPYNRQYWRVIMNENYSRPSFWVSDFRHRYLMRTGTDYQGQVPSSP QPGLYQGFSDVHKLLANHPKPQRESRHLPVLPMTPRIVFEHANEKRIDTAKRMRRDRRRIEELKTLEFWGWYMKLQRVRG RWCREQGVSSRGVYGPAVDAAELWG D0A7Z9 mL88 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.02225559 1 Trypanosoma brucei brucei LEVO MLQINAFKLVRATPFLLKRTGKPADTPDYKQVYLPYDAAPTERELERERRRFKQAYHGRMEHRKLVEVKEVPLNVYTYGK EGMSLPIAIFKDQKDPVIGPEWTYPGIYENKIAAQHWYTEELFDKESKEAFESPWQQQILDNQVKRRMAKVMFRMRQVNM KAVDLFQKERGSSRRSGGAGEKGKDGGGKK C9ZUW3 mL89 Trypanosoma brucei brucei 927/4 GUTat10.1 0.022213405999999998 1 Trypanosoma brucei brucei LEVO MSGAFGCGSYRSVVAGTQNVPRRMTFYPSAYELIQLHKAHREVIRHFYVRDKIFDNKFPGNALANGLFKFVPNRRENYHM RELMESIRRRSIWMHRIKQQREINAKVVENMEVKYGKKAAASMLCFTTPDSNAYFAPHRYQDVANSWPNYWQHPSVNHVV PKPRWRRHRELGGITRVEDPFAVQASDY Q57WW5 mL90 Trypanosoma brucei brucei 927/4 GUTat10.1 0.022123414 1 Trypanosoma brucei brucei LEVO MLRSRIPCVVVTLVQCRAMHGKPTQSHKVRTQHSRRWWTQSKARHLTAMPHDECKSRPHFPAYNEDVDRPMVVPPDAICF NCDKPIDAGDVNSYVWIPAGNATVPTPQGYFFHFGCFKCNRCKYRLGHNKFYSKDGKAWCIPCALGRDVRVPTRRWHTSY VNTHRTGSRLTGHFFPRHSHQMEFLFDPNS Q585P1 mS91 Trypanosoma brucei brucei 927/4 GUTat10.1 0.020618519999999998 1 Trypanosoma brucei brucei LEVO MLFTRCLLAVTTINSSTASAAGRLIRIRKKSKWIDRRSKRIPHNGKDVWQFGEQPSCALCHVRFRFKQDYEAHKESELHQ NRLRWVETMKWWEEIGEPHHQQHAASEWEWFRQRVLPAKAAAMGLSEEDAARELRRAVMHETPRWYSRIQPPNARSEIKE PRDQRWPSSPKW Q580U5 mL92 Trypanosoma brucei brucei 927/4 GUTat10.1 0.020542291

residues 46-64 built as polyalanines

1 Trypanosoma brucei brucei LEVO MRISERAFMDIAIGTKAPRRIVFKLFPRKCPSAVKNFIELCSGNVSTDTYESGNRDKLISESALPQLTYKNSTFHRVEKG YLIQGGDIVTGRGTEQLSIYGGTFSAPEEVRASVFDKPGLVGTASSSPNAHGSQFFILTAKEANHLNGTCICFGQVADGL DVVQEIEQVPIDPSGFPSLKVSIVDCGVLE Q383Y4 5.2.1.8 mL93 Trypanosoma brucei brucei MHOM/CI/86/DAL972 0.017343738 1 Trypanosoma brucei brucei LEVO FKQGQGMFSHQLKRLLQKKSIHRYNWDPLPMYDPRKLVHASRHMDVETWREVPDPHWDERSYLVPDQMFYNIPVPPEYKD AYWWRELQARRVQCPVEWVSHRMYNKGDRQRYDFQDLAFRKKFEFSYEEVVKNAKDMRS D0A4T0 mL94 Trypanosoma brucei brucei 927/4 GUTat10.1 0.017830958999999997 1 Trypanosoma brucei brucei LEVO MAQWVPKTAWKVSNLNKRYGPGYITSGFSSENTLGYTHMVHDSHKLREKLSSRKGIFVIDVRDPGERKLNPVPRSVALHH HDLLSGASCPILPQQKSAAELFVLASNESRGLNSAAALRRWGYDSVVQVDYNTLVEAGYVSRCDYATAGTKDSAEQAVSG ED Q38EP7 mL95 Trypanosoma brucei brucei 927/4 GUTat10.1 0.017803377 1 Trypanosoma brucei brucei LEVO MFRPTTAIADSFKEHYHRVHLPRRLALQRYARQQSLRNAAKGNVKAEEVPYKYNRWWVNEEHEFVHQYAFVEDPEVTKAK RETLPPVTRENIWKEPQQTFFLPFAPYVRVVDYPKDPDAKFLKPVNIPRWKDYMQRTKPVIPRTWY Q389K3 mL96 Trypanosoma brucei brucei 927/4 GUTat10.1 0.016387653999999998 1 Trypanosoma brucei brucei LEVO MNDIYARRLAQATMFHQLMRCHGTLWAATQVTKEQMDYNFIREEFMRVNGRRAMPLLLGAAANENLHQLHLSHLSEHCAW GESARALAVQRQTPLSQRVAALGRMAETIHQVKTASTVQNLFNEQISCMEGISSFEEEPLIEGE Q381W9 mL97 Trypanosoma brucei brucei 927/4 GUTat10.1 0.012974784999999999 1 Trypanosoma brucei brucei LEVO MSSRFFQKYFIRCGNCQTIQRYAKGYKPIPNPILFDSDAHCRSYHRERRDCTGLTGTLVTCRCDKCARVHSHWTVMDFQE FLDAKLVMTPEERTALLWPGAGSRAEPSSGTSN Q388L8 mL98 Trypanosoma brucei brucei 927/4 GUTat10.1 0.012968644 1 Trypanosoma brucei brucei LEVO MVLRGVRLRSVAVSCYGSSLTAATRCLSVRTEDFFSKEAISHARRVSWAPHTTEKKQGAFAKLARSNFGDPLPSSFAQEP YFEEEIEAHRKHHRPDVYIYKYNVSPTHFSLRE Q388M2 mL99 Trypanosoma brucei brucei 927/4 GUTat10.1 0.012334969 1 Trypanosoma brucei brucei LEVO MYQRTRFLWSSWRDYPLGSRDRRGRFNMDEAAAALQLNPAYAAALYRPLNYTFHIRGQLYPAQKGRPSRPGSLAASQGRM FPLYQRNDRLDKELFRLNSRGLTTE Q587H8 mL100 Trypanosoma brucei brucei 0.010600361999999999 1 Trypanosoma brucei brucei LEVO MALFSCFRCGYMYEFAVSNSYCRKLTLRNDHCPRCDQLTLFRFMSVSGMVGNMPFKPIGVPGPSYATLWWRKTREGKEAS APLDAVCKSDRW A0A1G4HYZ0 UNK Trypanosoma brucei brucei Lister 427 0.003932839

residues built as UNK

2 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.015081565

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.002060531

residues built as UNK

3 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.0014647969999999999

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) UNK Trypanosoma brucei brucei Lister 427 0.000869063

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.001294587

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.000528644

residues built as UNK

1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) UNK Trypanosoma brucei brucei Lister 427 0.0009541679999999999 1 Trypanosoma brucei brucei LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) ZINC ION 6.5409e-05 10 ZN SPERMIDINE 0.000145246 4 SPD URIDINE 5'-TRIPHOSPHATE 0.000484141 1 UTP MAGNESIUM ION 2.4305e-05 46 MG GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP SPERMINE 0.00020234 1 SPM NICOTINAMIDE-ADENINE-DINUCLEOTIDE 0.0006634249999999999 1 NAD SERINE 0.000105093 1 SER water 1.8015e-05 3 HOH singleParticle particle 7.4 ETHANE-PROPANE 98 278 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) INTEGRATING 40.0 1

initial model from 2D class averages in EMAN

FOURIER SPACE 7.8 FSC 0.143 CUT-OFF RELION 7141 OTHER MAXIMUM LIKELIHOOD RELION emd_0229.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 556.0 556.0 556.0 90.0 90.0 90.0 X Y Z -0.089613646 0.18272288 0.0019386684 0.011937334 1.39 1.39 1.39 0.054 AUTHOR ::::EMDATABANK.org::::EMD-0229:::: map of the complete T. brucei mitoribosome RIGID BODY FIT emd_0229_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 556.0 556.0 556.0 90.0 90.0 90.0 X Y Z -0.0585492 0.09553239 0.0005140252 0.008288807 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-0229:::: half map (even) of the complete T. brucei mitoribosome emd_0229_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 556.0 556.0 556.0 90.0 90.0 90.0 X Y Z -0.06064109 0.092460014 0.0005596022 0.008265333 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-0229:::: half map (odd) of the complete T. brucei mitoribosome