<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_9_3/emdb.xsd" version="3.0.9.3" emdb_id="EMD-0212">
    <admin>
        <current_status>
            <date>2024-05-15</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2018-08-20</deposition>
            <header_release>2018-12-26</header_release>
            <map_release>2018-12-26</map_release>
            <update>2024-05-15</update>
        </key_dates>
        <title>PAN-proteasome in state 1</title>
        <authors_list>
            <author>Majumder P</author>
            <author>Rudack T</author>
        </authors_list>
        <keywords>PAN, Proteasome, AAA-ATPase, Archaea, hydrolase</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Majumder P</author>
                    <author order="2">Rudack T</author>
                    <author order="3">Beck F</author>
                    <author order="4">Danev R</author>
                    <author order="5">Pfeifer G</author>
                    <author order="6">Nagy I</author>
                    <author order="7">Baumeister W</author>
                    <title>Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle.</title>
                    <journal_abbreviation>Proc. Natl. Acad. Sci. U.S.A.</journal_abbreviation>
                    <country>US</country>
                    <volume>116</volume>
                    <first_page>534</first_page>
                    <last_page>539</last_page>
                    <year>2019</year>
                    <external_references type="PUBMED">30559193</external_references>
                    <external_references type="DOI">doi:10.1073/pnas.1817752116</external_references>
                    <external_references type="ISSN">1091-6490</external_references>
                    <external_references type="CSD">0040</external_references>
                    <external_references type="ASTM">PNASA6</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6he8</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>PAN-proteasome</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>PAN-proteasome</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="224325">Archaeoglobus fulgidus DSM 4304</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">1.25</theoretical>
                </molecular_weight>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>20S-proteasome</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="224325">Archaeoglobus fulgidus DSM 4304</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>Proteasome-activating nucleotidase (PAN)</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="224325">Archaeoglobus fulgidus DSM 4304</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Proteasome subunit alpha</name>
                <natural_source database="NCBI">
                    <organism ncbi="224325">Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)</organism>
                    <strain>ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.02715616</theoretical>
                </molecular_weight>
                <number_of_copies>14</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>QMGYDRAITVFSPDGRLFQVEYAREAVKRGATAIGIKCKEGVILIADKRVGSKLLEADTIEKIYKIDEHICAATSGLVAD
ARVLIDRARIEAQINRLTYDEPITVKELAKKICDFKQQYTQYGGVRPFGVSLLIAGVDEVPKLYETDPSGALLEYKATAI
GMGRNAVTEFFEKEYRDDLSFDDAMVLGLVAMGLSIESELVPENIEVGYVKVDDRTFKEVSPEELKPYVERANERIRELL
KK</string>
                    <external_references type="UNIPROTKB">O29760</external_references>
                </sequence>
                <ec_number>3.4.25.1</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Proteasome subunit beta</name>
                <natural_source database="NCBI">
                    <organism ncbi="224325">Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)</organism>
                    <strain>ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.022132283</theoretical>
                </molecular_weight>
                <number_of_copies>14</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>TTTVGLVCKDGVVMATEKRATMGNFIASKAAKKIYQIADRMAMTTAGSVGDAQFLARIIKIEANLYEIRRERKPTVRAIA
TLTSNLLNSYRYFPYLVQLLIGGIDSEGKSIYSIDPIGGAIEEKDIVATGSGSLTAYGVLEDRFTPEIGVDEAVELAVRA
IYSAMKRDSASGDGIDVVKITEDEFYQYSPEEVEQILAKFRK</string>
                    <external_references type="UNIPROTKB">Q9P996</external_references>
                </sequence>
                <ec_number>3.4.25.1</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Proteasome-activating nucleotidase</name>
                <natural_source database="NCBI">
                    <organism ncbi="224325">Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)</organism>
                    <strain>ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.044102976999999995</theoretical>
                </molecular_weight>
                <number_of_copies>6</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>LLEKLKKLEEDYYKLRELYRRLEDEKKFIESERIRYEREVRRLRSEVERLRSPPLLVGVVSDILEDGRVVVKSSTGPKFV
VNTSQYINEEELKPGARVALNQQTLAIVNVLPTSKDPMVYGFEVEEKPEVSYEDIGGLDVQIEEIREAVELPLLKPELFA
EVGIEPPKGVLLYGPPGTGKTLLAKAVANQTRATFIRVVGSEFVQKYIGEGARLVREVFQLAKEKAPSIIFIDELDAIAA
RRTNSDTSGDREVQRTMMQLLAELDGFDPRGDVKVIGATNRIDILDPAILRPGRFDRIIEVPLPTFEGRIQIFKIHTRKM
KLAEDVDFKELARITEGASGADIKAICTEAGMFAIREERAKVTMLDFTKAIEKVLKKTTPIPDLKGVMFV</string>
                    <external_references type="UNIPROTKB">O28303</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="4">
                <name>ADENOSINE-5'-TRIPHOSPHATE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000507181</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <formula>ATP</formula>
            </ligand>
            <ligand macromolecule_id="5">
                <name>MAGNESIUM ION</name>
                <molecular_weight>
                    <theoretical units="MDa">2.4305e-05</theoretical>
                </molecular_weight>
                <number_of_copies>5</number_of_copies>
                <formula>MG</formula>
            </ligand>
            <ligand macromolecule_id="6">
                <name>ADENOSINE-5'-DIPHOSPHATE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000427201</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <formula>ADP</formula>
            </ligand>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.1</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE-PROPANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>SUPER-RESOLUTION</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">30.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="EMDB MAP"/>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">6.86</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                    <number_images_used>38230</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="226493">
        <file>emd_0212.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>384</col>
            <row>384</row>
            <sec>384</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>384</x>
            <y>384</y>
            <z>384</z>
        </spacing>
        <cell>
            <a units="Å">514.56</a>
            <b units="Å">514.56</b>
            <c units="Å">514.56</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.03647783</minimum>
            <maximum>0.043262266</maximum>
            <average>0.000027298773</average>
            <std>0.0013231856</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.34</x>
            <y units="Å">1.34</y>
            <z units="Å">1.34</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.006</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0212::::</label>
        <annotation_details>Pseudo-single capped PAN-proteasome in state 1</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>FLEXIBLE FIT</refinement_protocol>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
        <additional_map_list>
            <additional_map format="CCP4" size_kbytes="226493">
                <file>emd_0212_additional_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>384</col>
                    <row>384</row>
                    <sec>384</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>384</x>
                    <y>384</y>
                    <z>384</z>
                </spacing>
                <cell>
                    <a units="Å">514.56</a>
                    <b units="Å">514.56</b>
                    <c units="Å">514.56</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.039748225</minimum>
                    <maximum>0.054464325</maximum>
                    <average>0.0000073352267</average>
                    <std>0.0009118389</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.34</x>
                    <y units="Å">1.34</y>
                    <z units="Å">1.34</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-0212::::</label>
                <annotation_details>AAAob of PAN-proteasome in state 1</annotation_details>
            </additional_map>
        </additional_map_list>
    </interpretation>
</emd>
