2019-03-20 REL PDBe PDBe PDBe 2018-08-10 2018-10-24 2019-03-20 2019-03-20 Echovirus 18 Open particle without three pentamers Buchta D Fuzik T Hrebik D Levdansky Y Moravcova J Plevka P Buchta D Fuzik T Hrebik D Levdansky Y Sukenik L Mukhamedova L Moravcova J Vacha R Plevka P Enterovirus particles expel capsid pentamers to enable genome release. Nat Commun UK 10 1138 1138 2019 30850609 doi:10.1038/s41467-019-09132-x 2041-1723 EMD-0187 associated EM volume Echovirus E18 Echovirus E18 0 1 Echovirus E18 Metcalf Homo sapiens 6.32 Capsid 340.0 1 VIRION STRAIN false false singleParticle particle 0.5 6.0 37.5 MES 2-(N-Morpholino)ethanesulfonic acid 25.0 NaCl sodium chloride 5.0 Tris 2-Amino-2-(hydroxymethyl)-1,3-propanediol Quantifoil R2/1 COPPER 300 FORMVAR HOLEY GLOW DISCHARGE OTHER ETHANE 100 298 FEI VITROBOT MARK IV
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.0 0.651 3.0 3.282 75000. 79725. FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON III (4k x 4k) INTEGRATING 4096 4096 14.0 1 6331 1.0 45.2
Images were collected in movie-mode at 39 frames per second
 1 509565 Gctf EMD-0182
Echovirus 18 A-particle
 2 C1 BACK PROJECTION 4.85 FSC 0.143 CUT-OFF RELION 2.1 13635 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 5 2728.0 RELION 2.1 emd_0187.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 543.232 543.232 543.232 90.0 90.0 90.0 X Y Z -0.13227849 0.26092762 0.0013057424 0.013276415 1.061 1.061 1.061 0.06 AUTHOR Echovirus 18 Open particle without three pentamers - C1 symmetry 116.769999999999996