2024-11-06 REL PDBe PDBe PDBe 2018-08-10 2018-09-12 2019-03-20 2024-11-06 European Research Council 335855 Czech Republic Echovirus 18 Open particle without one pentamer Buchta D Fuzik T echovirus, echovirus 18, open particle, O-particle, enterovirus, picornavirus, genome release, VIRUS Buchta D Fuzik T Hrebik D Levdansky Y Sukenik L Mukhamedova L Moravcova J Vacha R Plevka P Enterovirus particles expel capsid pentamers to enable genome release. Nat Commun UK 10 1138 1138 2019 30850609 doi:10.1038/s41467-019-09132-x 2041-1723 6hbk FULLOVERLAP Echovirus E18 Echovirus E18 0 1 2 3 Echovirus E18 Metcalf Homo sapiens 7.20 Capsid 340.0 1 VIRION STRAIN false false Echovirus 18 capsid protein 1 Echovirus E18 0.032564445 11 LEVO GDNQDRTVANTQPSGPSNSTEIPALTAVETGHTSQVDPSDTIQTRHVVNFHSRSESTIENFMGRAACVFMDQYKINGEET STDRFAVWTINIREMAQLRRKCEMFTYMRFDIEMTMVITSCQDQGTILDQDMPVLTHQIMYVPPGGPIPAKVDGYEWQTS TNPSVFWTEGNAPPRISIPFISVGNAYSSFYDGWSHFTQDGTYGYTTLNAMGKLYIRHVNRSSPHQITSTIRVYFKPKHI KAWVPRPPRLCPYINKRDVNFVVTEITDSRTSITDTPHPEHSVLATH Q8V635 Echovirus 18 capsid protein 2 Echovirus E18 0.026143782999999997 11 LEVO GVPVLNTPGSNQFLTSDDYQSPSAMPQFDETPEMHIPGEVRNLMEIAEVDSVVPVNNVTGKTKSMDAYQIPVGTGNTDKT KPIFSFQMDPGYSSVLKRTLLGEMLNYYAHWSGSVKLTFLFCGSAMATGKLLISYSPPGASVPTSRKDAMLGTHIVWDIG LQSSCVLCVPWISQSHYRMVQQDPYTSAGYITCWYQTNIVVPPGAPTSCDVLCFASACNDFSVRLLRDTPFMAQPGKLQ Q8V635 Echovirus 18 capsid protein 3 Echovirus E18 0.028674199 11 LEVO SPSAEECGYSDRVRSMTLGNSTITTQESANVVVGYGEWPSYLSDREATAEDQPTQPDVATCRFYTLESVQWEKTSPGWWW KFPEALKNMGLFGQNMHYHYLGRAGYTIHVQCNASKFHQGCLLVVCVPEAEMGCADTDTTFPATELTTEDTPHVFTSDSI TGKKVQAAVCNAGMGVGVGNLTIFPHQWINLRTNNSATIVIPYINSVPMDNMFRHYNFTLMIIPFAPLNFTDGATAYVPI TVTIAPMYAEYNGLRLAST Q8V635 singleParticle particle 0.5 6.0 37.5 MES 2-(N-Morpholino)ethanesulfonic acid 5.0 Tris 2-Amino-2-(hydroxymethyl)-1,3-propanediol 25.0 NaCl sodium chloride Quantifoil R2/1 COPPER 300 FORMVAR HOLEY GLOW DISCHARGE OTHER ETHANE 100 298 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.0 0.651 3.0 3.282 75000.0 79575.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON III (4k x 4k) INTEGRATING 4096 4096 1 6331 1.0 45.2
Images were collected in movie-mode at 39 frames per second
 1 509565 EMD-0182
Echovirus 18 A-particle
 1 C5 BACK PROJECTION 3.8 FSC 0.143 CUT-OFF RELION 2.1 3563 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 5 3482.0 RELION 2.1 emd_0185.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 -175 -175 -175 350 350 350 372.05 372.05 372.05 90.0 90.0 90.0 X Y Z -0.14749883 0.30619463 0.004718978 0.01935793 1.063 1.063 1.063 0.06 AUTHOR Echovirus 18 Open particle without one pentamer 6HBH PDB experimental model RIGID BODY FIT
Group B-factor refinement
R-factor RECIPROCAL 131.319999999999993 emd_0185_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 544.256 544.256 544.256 90.0 90.0 90.0 X Y Z -0.036921564 0.06408049 -0.00034678966 0.005379132 1.063 1.063 1.063 AUTHOR Echovirus 18 Open particle without one pentamer half maps emd_0185_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 544.256 544.256 544.256 90.0 90.0 90.0 X Y Z -0.041576326 0.06437197 -0.00033656665 0.00532962 1.063 1.063 1.063 AUTHOR Echovirus 18 Open particle without one pentamer half maps