Cryo-EM structure of a 70S Bacillus subtilis ribosome translating the ErmD leader peptide in complex with telithromycin

2024-11-20 REL PDBe PDBe PDBe 2018-08-07 2016-12-21 2018-08-29 2024-11-20 German Research Foundation FOR1805 Germany German Research Foundation WI3285/6-1 Germany Ministry of Education (Czech Republic) CIISB project number LM2015043 Czech Republic European Union iNext project number 2992 Czech Republic Cryo-EM structure of a 70S Bacillus subtilis ribosome translating the ErmD leader peptide in complex with telithromycin Crowe-McAuliffe C Graf M single particle cryo-EM, stalling peptide, ribosome, telithromycin Crowe-McAuliffe C Graf M Huter P Takada H Abdelshahid M Novacek J Murina V Atkinson GC Hauryliuk V Wilson DN Structural basis for antibiotic resistance mediated by theBacillus subtilisABCF ATPase VmlR. Proc. Natl. Acad. Sci. U.S.A. US 115 8978 8983 2018 30126986 doi:10.1073/pnas.1808535115 1091-6490 0040 PNASA6 EMD-0177 other EM volume

The same complex with vmlR bound

6ha1 FULLOVERLAP 70S ribosome with P-tRNA translating the ErmD stalling peptide and complexed with telithromycin 70S ribosome with P-tRNA translating the ErmD stalling peptide and complexed with telithromycin 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 70S ribosome 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Bacillus subtilis (strain 168) ErmD stalling peptide 1 30 Bacillus subtilis (strain 168) tRNA 1 51 Escherichia coli 23S ribosomal RNA Bacillus subtilis (strain 168) 0.9496463119999999 1 GGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGGACGAACACCGAUAUGCUUCG GGGAGCUGUAAGCAAGCUUUGAUCCGGAGAUUUCCGAAUGGGGAAACCCACCACUCGUAAUGGAGUGGUAUCCAUAUCUG AAUUCAUAGGAUAUGAGAAGGCAGACCCGGGGAACUGAAACAUCUAAGUACCCGGAGGAAGAGAAAGCAAAUGCGAUUCC CUGAGUAGCGGCGAGCGAAACGGGAUCAGCCCAAACCAAGAGGCUUGCCUCUUGGGGUUGUAGGACACUCUGUACGGAGU UACAAAGGAACGAGGUAGAUGAAGAGGUCUGGAAAGGCCCGCCAUAGGAGGUAACAGCCCUGUAGUCAAAACUUCGUUCU CUCCUGAGUGGAUCCUGAGUACGGCGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUAC UCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAGAGAUCCUGAA ACCGUGUGCCUACAAGUAGUCAGAGCCCGUUAACGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUCU CGUGCAAGGUUAAGCAGAAGAUGCGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGCAUGAGUACGUGGUCGUAGACCC GAAACCAGGUGAUCUACCCAUGUCCAGGGUGAAGUUCAGGUAACACUGAAUGGAGGCCCGAACCCACGCACGUUGAAAAG UGCGGGGAUGAGGUGUGGGUAGGGGUGAAAUGCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGC UAGCCUCAAGGUAAGAGUCUUGGAGGUAGAGCACUGAUUGGACUAGGGGCCCCUACCGGGUUACCGAAUUCAGUCAAACU CCGAAUGCCAAUGACUUAUCCUUGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAAAGGGAAACAGCCCAGACCGC CAGCUAAGGUCCCAAAGUAUACGUUAAGUGGAAAAGGAUGUGGAGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAG CCACCAUUUAAAGAGUGCGUAAUAGCUCACUGGUCGAGUGACUCUGCGCCGAAAAUGUACCGGGGCUAAACGUAUCACCG AAGCUGCGGACUGUUCUUCGAACAGUGGUAGGAGAGCGUUCUAAGGGCUGUGAAGCCAGACCGGAAGGACUGGUGGAGCG CUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAGGGGUGAGAAUCCCCUCCACCGAAUGCCUAAGGUUUCCUGAGGA AGGCUCGUCCGCUCAGGGUUAGUCGGGACCUAAGCCGAGGCCGAAAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCCUG UACCACCUCCUCACCAUUUGAGCAAUGGGGGGACGCAGGAGGAUAGGGUAAGCGCGGUAUUGGAUAUCCGCGUCCAAGCA GUUAGGCUGGGAAAUAGGCAAAUCCGUUUCCCAUAAGGCUGAGCUGUGAUGGCGAGCGAAAUAUAGUAGCGAAGUUCCUG AUUCCACACUGCCAAGAAAAGCCUCUAGCGAGGUGAGAGGUGCCCGUACCGCAAACCGACACAGGUAGGCGAGGAGAGAA UCCUAAGGUGAUCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUCUGUUA GGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGCGACUGUUUAGCAAAAACACAGGUCUCUGCGAAGCCGUAAG GCGAAGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGCGCUUAGCGUAAGCGAAGGUGCGAAUUGAAG CCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAA GGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACA GGACGGAAAGACCCCGUGGAGCUUUACUGCAGCCUGAUAUUGAAUGUUGGUACAGCUUGUACAGGAUAGGUAGGAGCCUU GGAAACCGGAGCGCCAGCUUCGGUGGAGGCAUCGGUGGGAUACUACCCUGGCUGUAUUGACCUUCUAACCCGCCGCCCUU AUCGGGCGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGU UCCCUCAGAAUGGUUGGAAAUCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGG GACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAA CAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUA GUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCC CUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACGCACCGCUGGUGUAC CAGUUGUUCUGCCAAGGGCAUCGCUGGGUAGCUAUGUGCGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCC CUCAAGAUGAGAUUUCCCAUUCCGCAAGGAAGUAAGAUCCCUGAAAGAUGAUCAGGUUGAUAGGUCUGAGGUGGAAGUGU GGCGACACAUGGAGCUGACAGAUACUAAUCGAUCGAGGACUUAACCAU D26185.1 5S ribosomal RNA Bacillus subtilis (strain 168) 0.03615752 1 UGGUGGCGAUAGCGAAGAGGUCACACCCGUUCCCAUACCGAACACGGAAGUUAAGCUCUUCAGCGCCGAUGGUAGUCGGG GGUUUCCCCCUGUGAGAGUAGGACGCCGCCAA CP019662.1 50S ribosomal protein L2 Bacillus subtilis (strain 168) 0.030335124999999998 1 LEVO MAIKKYKPTSNGRRGMTTSDFAEITTDKPEKSLLAPLHKKGGRNNQGKLTVRHQGGGHKRQYRVIDFKRDKDGIPGRVAT VEYDPNRSANIALINYADGEKRYILAPKGIQVGTEIMSGPEADIKVGNALPLINIPVGTVVHNIELKPGKGGQLVRSAGT SAQVLGKEGKYVLVRLNSGEVRMILSACRASIGQVGNEQHELINIGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAP IGRKSPMSPWGKPTLGFKTRKKKNKSDKFIVRRRKNK P42919 50S ribosomal protein L3 Bacillus subtilis (strain 168) 0.022723348 1 LEVO MTKGILGRKIGMTQVFAENGDLIPVTVIEAAPNVVLQKKTAENDGYEAIQLGFDDKREKLSNKPEKGHVAKAETAPKRFV KELRGVEMDAYEVGQEVKVEIFSAGEIVDVTGVSKGKGFQGAIKRHGQSRGPMSHGSRYHRRPGSMGPVDPNRVFKGKLL PGRMGGEQITVQNLEIVKVDAERNLLLIKGNVPGAKKSLITVKSAVKSK P42920 50S ribosomal protein L4 Bacillus subtilis (strain 168) 0.022424951 1 LEVO MPKVALYNQNGSTAGDIELNASVFGIEPNESVVFDAILMQRASLRQGTHKVKNRSEVRGGGRKPWRQKGTGRARQGSIRS PQWRGGGVVFGPTPRSYSYKLPKKVRRLAIKSVLSSKVIDNNIIVLEDLTLDTAKTKEMAAILKGLSVEKKALIVTADAN EAVALSARNIPGVTVVEANGINVLDVVNHEKLLITKAAVEKVEEVLA P42921 50S ribosomal protein L5 Bacillus subtilis (strain 168) 0.020177564 1 LEVO MNRLKEKYNKEIAPALMTKFNYDSVMQVPKIEKIVINMGVGDAVQNAKAIDSAVEELTFIAGQKPVVTRAKKSIAGFRLR EGMPIGAKVTLRGERMYDFLDKLISVSLPRVRDFRGVSKKSFDGRGNYTLGIKEQLIFPEIDYDKVTKVRGMDIVIVTTA NTDEEARELLTQVGMPFQK P12877 50S ribosomal protein L6 Bacillus subtilis (strain 168) 0.019543388999999998 1 LEVO MSRVGKKLLEIPSDVTVTLNDNNTVAVKGPKGELTRTFHPDMEIKVEDNVLTVARPSDQKEHRALHGTTRSLLGNMVEGV SKGFERGLELVGVGYRASKSGNKLVLNVGYSHPVEIVPEEGIEIEVPSQTKVVVKGTDKERVGAIAANIRAVRSPEPYKG KGIRYEGEVVRRKEGKSAK P46898 50S ribosomal protein L13 Bacillus subtilis (strain 168) 0.016407104 1 LEVO MRTTPMANASTIERKWLVVDAAGKTLGRLSSEVAAILRGKHKPTYTPHVDTGDHVIIINAEKIELTGKKLTDKIYYRHTQ HPGGLKSRTALEMRTNYPEKMLELAIKGMLPKGSLGRQMFKKLNVYRGSEHPHEAQKPEVYELRG P70974 50S ribosomal protein L14 Bacillus subtilis (strain 168) 0.013175288 1 LEVO MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVKQATPGGVVKKGEVVKAVIVRTKSGARRSDGSYISFD ENACVIIRDDKSPRGTRIFGPVARELRENNFMKIVSLAPEVI P12875 50S ribosomal protein L15 Bacillus subtilis (strain 168) 0.015410693999999999 1 LEVO MKLHELKPSEGSRKTRNRVGRGIGSGNGKTAGKGHKGQNARSGGGVRPGFEGGQMPLFQRLPKRGFTNINRKEYAVVNLD KLNGFAEGTEVTPELLLETGVISKLNAGVKILGNGKLEKKLTVKANKFSASAKEAVEAAGGTAEVI P19946 50S ribosomal protein L16 Bacillus subtilis (strain 168) 0.016223049 1 LEVO MLLPKRVKYRREHRGKMRGRAKGGTEVHFGEFGIQALEASWITNRQIEAARIAMTRYMKRGGKVWIKIFPSKPYTAKPLE VRMGSGKGAPEGWVAVVKPGKVLFEISGVSEEVAREALRLASHKLPIKTKFVKREEIGGESNES P14577 50S ribosomal protein L17 Bacillus subtilis (strain 168) 0.013774806 1 LEVO MSYRKLGRTSAQRKAMLRDLTTDLIINERIETTETRAKELRSVVEKMITLGKRGDLHARRQAAAYIRNEVANEENNQDAL QKLFSDIATRYEERQGGYTRIMKLGPRRGDGAPMAIIELV P20277 50S ribosomal protein L18 Bacillus subtilis (strain 168) 0.012993828999999998 1 LEVO MITKTSKNAARLKRHARVRAKLSGTAERPRLNVFRSNKHIYAQIIDDVNGVTLASASTLDKDLNVESTGDTSAATKVGEL VAKRAAEKGISDVVFDRGGYLYHGRVKALADAAREAGLKF P46899 50S ribosomal protein L19 Bacillus subtilis (strain 168) 0.013416852999999998 1 LEVO MQKLIEDITKEQLRTDLPAFRPGDTLRVHVKVVEGNRERIQIFEGVVIKRRGGGISETFTVRKISYGVGVERTFPVHTPK IAKIEVVRYGKVRRAKLYYLRELRGKAARIKEIRR O31742 50S ribosomal protein L20 Bacillus subtilis (strain 168) 0.013669189 1 LEVO MPRVKGGTVTRKRRKKVLKLAKGYFGSKHTLYKVANQQVMKSGNYAFRDRRQKKRDFRKLWITRINAAARMNGLSYSRLM HGLKLSGIEVNRKMLADLAVNDLTAFNQLADAAKAQLNK P55873 50S ribosomal protein L21 Bacillus subtilis (strain 168) 0.011296081 1 LEVO MYAIIKTGGKQIKVEEGQTVYIEKLAAEAGETVTFEDVLFVGGDNVKVGNPTVEGATVTAKVEKQGRAKKITVFRYKPKK NVHKKQGHRQPYTKVTIEKINA P26908 50S ribosomal protein L22 Bacillus subtilis (strain 168) 0.012481608 1 LEVO MQAKAVARTVRIAPRKARLVMDLIRGKQVGEAVSILNLTPRAASPIIEKVLKSAIANAEHNYEMDANNLVISQAFVDEGP TLKRFRPRAMGRASQINKRTSHITIVVSEKKEG P42060 50S ribosomal protein L23 Bacillus subtilis (strain 168) 0.010978813 1 LEVO MKDPRDVLKRPVITERSADLMTEKKYTFEVDVRANKTEVKDAVESIFGVKVDKVNIMNYKGKSKRVGRYTGMTSRRRKAI VKLTADSKEIEIFEA P42924 50S ribosomal protein L24 Bacillus subtilis (strain 168) 0.01116612 1 LEVO MHVKKGDKVMVISGKDKGKQGTILAAFPKKDRVLVEGVNMVKKHSKPTQANPQGGISNQEAPIHVSNVMPLDPKTGEVTR VGYKVEDGKKVRVAKKSGQVLDK P0CI78 50S ribosomal protein L27 Bacillus subtilis (strain 168) 0.010391854999999998 1 LEVO MLRLDLQFFASKKGVGSTKNGRDSEAKRLGAKRADGQFVTGGSILYRQRGTKIYPGENVGRGGDDTLFAKIDGTVKFERF GRDRKKVSVYPVAQ P05657 50S ribosomal protein L28 Bacillus subtilis (strain 168) 0.006826144 1 LEVO MARKCVITGKKTTAGNNRSHAMNASKRTWGANLQKVRILVNGKPKKVYVSARALKSGKVERV P37807 50S ribosomal protein L29 Bacillus subtilis (strain 168) 0.007728029 1 LEVO MKANEIRDLTTAEIEQKVKSLKEELFNLRFQLATGQLENTARIREVRKAIARMKTVIREREIAANK P12873 50S ribosomal protein L30 Bacillus subtilis (strain 168) 0.006650795 1 LEVO MAKLEITLKRSVIGRPEDQRVTVRTLGLKKTNQTVVHEDNAAIRGMINKVSHLVSVKEQ P19947 50S ribosomal protein L32 Bacillus subtilis (strain 168) 0.006745073 1 LEVO MAVPFRRTSKMKKRLRRTHFKLNVPGMTECPSCGEMKLSHRVCKACGSYNGKDINVKSN O34687 50S ribosomal protein L33 1 Bacillus subtilis (strain 168) 0.005915875 1 LEVO MRVNITLACTECGERNYISKKNKRNNPDRVEFKKYCPRDKKSTLHRETK P56849 50S ribosomal protein L34 Bacillus subtilis (strain 168) 0.005271332 1 LEVO MKRTFQPNNRKRSKVHGFRSRMSSKNGRLVLARRRRKGRKVLSA P05647 50S ribosomal protein L35 Bacillus subtilis (strain 168) 0.0075811279999999995 1 LEVO MPKMKTHRGSAKRFKKTGSGKLKRSHAYTSHLFANKSQKQKRKLRKSAVVSAGDFKRIKQMLANIK P55874 50S ribosomal protein L36 Bacillus subtilis (strain 168) 0.004318422 1 LEVO MKVRPSVKPICEKCKVIRRKGKVMVICENPKHKQKQG P20278 50S ribosomal protein L31 Bacillus subtilis (strain 168) 0.007159191 1 LEVO MKAGIHPNFKKATVKCACGNEFETGSVKEEVRVEICSECHPFYTGRQKFASADGRVDRFNKKY Q03223 mRNA Bacillus subtilis (strain 168) 0.000872556

CUU

1 CUU 16S ribosomal RNA Bacillus subtilis (strain 168) 0.503369125 1 UUUAUCGGAGAGUUUGAUCCUGGCUCAGGACGAACGCUGGCGGCGUGCCUAAUACAUGCAAGUCGAGCGGACAGAUGGGA GCUUGCUCCCUGAUGUUAGCGGCGGACGGGUGAGUAACACGUGGGUAACCUGCCUGUAAGACUGGGAUAACUCCGGGAAA CCGGGGCUAAUACCGGAUGGUUGUUUGAACCGCAUGGUUCAAACAUAAAAGGUGGCUUCGGCUACCACUUACAGAUGGAC CCGCGGCGCAUUAGCUAGUUGGUGAGGUAACGGCUCACCAAGGCGACGAUGCGUAGCCGACCUGAGAGGGUGAUCGGCCA CACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUAGGGAAUCUUCCGCAAUGGACGAAAGUCUGACGGA GCAACGCCGCGUGAGUGAUGAAGGUUUUCGGAUCGUAAAGCUCUGUUGUUAGGGAAGAACAAGUGCCGUUCGAAUAGGGC GGUACCUUGACGGUACCUAACCAGAAAGCCACGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGUGGCAAGCGUU GUCCGGAAUUAUUGGGCGUAAAGGGCUCGCAGGCGGUUUCUUAAGUCUGAUGUGAAAGCCCCCGGCUCAACCGGGGAGGG UCAUUGGAAACUGGGGAACUUGAGUGCAGAAGAGGAGAGUGGAAUUCCACGUGUAGCGGUGAAAUGCGUAGAGAUGUGGA GGAACACCAGUGGCGAAGGCGACUCUCUGGUCUGUAACUGACGCUGAGGAGCGAAAGCGUGGGGAGCGAACAGGAUUAGA UACCCUGGUAGUCCACGCCGUAAACGAUGAGUGCUAAGUGUUAGGGGGUUUCCGCCCCUUAGUGCUGCAGCUAACGCAUU AAGCACUCCGCCUGGGGAGUACGGUCGCAAGACUGAAACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUG UGGUUUAAUUCGAAGCAACGCGAAGAACCUUACCAGGUCUUGACAUCCUCUGACAAUCCUAGAGAUAGGACGUCCCCUUC GGGGGCAGAGUGACAGGUGGUGCAUGGUUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAA CCCUUGAUCUUAGUUGCCAGCAUUCAGUUGGGCACUCUAAGGUGACUGCCGGUGACAAACCGGAGGAAGGUGGGGAUGAC GUCAAAUCAUCAUGCCCCUUAUGACCUGGGCUACACACGUGCUACAAUGGACAGAACAAAGGGCAGCGAAACCGCGAGGU UAAGCCAAUCCCACAAAUCUGUUCUCAGUUCGGAUCGCAGUCUGCAACUCGACUGCGUGAAGCUGGAAUCGCUAGUAAUC GCGGAUCAGCAUGCCGCGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCACGAGAGUUUGUAACACCCG AAGUCGGUGAGGUAACCUUUUAGGAGCCAGCCGCCGAAGGUGGGACAGAUGAUUGGGGUGAAGUCGUAACAAGGUAGCCG UAUCGGAAGGUGCGGCUGGAUCACCUCCUUUCUA AL009126.3 30S ribosomal protein S2 Bacillus subtilis (strain 168) 0.028009297 1 LEVO MSVISMKQLLEAGVHFGHQTRRWNPKMKRYIFTERNGIYIIDLQKTVKKVEEAYNFTKNLAAEGGKILFVGTKKQAQDSV KEEAQRSGMYYVNQRWLGGTLTNFETIQKRIKRLKDIEKMQENGTFDVLPKKEVVQLKKELERLEKFLGGIKDMKDLPDA LFIIDPRKERIAVAEARKLNIPIIGIVDTNCDPDEIDVVIPANDDAIRAVKLLTSKMADAILEAKQGEEEAEVAEETAPE TETTTA P21464 30S ribosomal protein S3 Bacillus subtilis (strain 168) 0.024364887 1 LEVO MGQKVNPVGLRIGVIRDWESKWYAGKDYADFLHEDLKIREYISKRLSDASVSKVEIERAANRVNITIHTAKPGMVIGKGG SEVEALRKALNSLTGKRVHINILEIKRADLDAQLVADNIARQLENRVSFRRAQKQQIQRTMRAGAQGVKTMVSGRLGGAD IARSEYYSEGTVPLHTLRADIDYATSEADTTYGKLGVKVWIYRGEVLPTKKKNEEGGK P21465 30S ribosomal protein S4 Bacillus subtilis (strain 168) 0.022874270999999998 1 LEVO MARYTGPSWKLSRRLGISLSGTGKELEKRPYAPGPHGPGQRKKLSEYGLQLQEKQKLRHMYGVNERQFRTLFDKAGKLAG KHGENFMILLDSRLDNVVYKLGLARTRRQARQLVNHGHILVDGSRVDIPSYLVKPGQTIGVREKSRNLSIIKESVEVNNF VPEYLTFDAEKLEGTFTRLPERSELAPEINEALIVEFYSR P21466 30S ribosomal protein S5 Bacillus subtilis (strain 168) 0.017650625 1 LEVO MRRIDPSKLELEERLVTVNRVAKVVKGGRRFRFAALVVVGDKNGHVGFGTGKAQEVPEAIRKAVEDAKKNLIEVPMVGTT IPHEIIGRFGAGNILLKPASEGTGVIAGGPVRAVLELAGVADILSKSLGSNTPINMIRATLQGLSELKRAEDVAKLRGKS VEELLG P21467 30S ribosomal protein S6 Bacillus subtilis (strain 168) 0.011140548 1 LEVO MRKYEVMYIIRPNIDEESKKAVIERFNNVLTSNGAEITGTKDWGKRRLAYEINDFRDGFYQIVNVQSDAAAVQEFDRLAK ISDDIIRHIVVKEEE P21468 30S ribosomal protein S7 Bacillus subtilis (strain 168) 0.017915879 1 LEVO MPRKGPVAKRDVLPDPIYNSKLVSRLINKMMIDGKKGKSQTILYKSFDIIKERTGNDAMEVFEQALKNIMPVLEVKARRV GGANYQVPVEVRPERRTTLGLRWLVNYARLRGEKTMEERLANEILDAANNTGAAVKKREDTHKMAEANKAFAHYRW P21469 30S ribosomal protein S8 Bacillus subtilis (strain 168) 0.014901426999999998 1 LEVO MVMTDPIADMLTRIRNANMVRHEKLEIPASKLKREIAEILKREGFIRDVEFVEDSKQGIIRVFLKYGQNNERVITGLKRI SKPGLRVYAKSNEVPRVLNGLGIAIISTSQGVLTDKEARAKQAGGEVLAYVW P12879 30S ribosomal protein S9 Bacillus subtilis (strain 168) 0.014335504 1 LEVO MAQVQYYGTGRRKSSVARVRLVPGEGRIVVNNREISEHIPSAALIEDIKQPLTLTETAGTYDVLVNVHGGGLSGQAGAIR HGIARALLEADPEYRTTLKRAGLLTRDARMKERKKYGLKGARRAPQFSKR P21470 30S ribosomal protein S10 Bacillus subtilis (strain 168) 0.011687661 1 LEVO MAKQKIRIRLKAYDHRILDQSAEKIVETAKRSGASVSGPIPLPTEKSVYTILRAVHKYKDSREQFEMRTHKRLIDIVNPT PQTVDALMRLDLPSGVDIEIKL P21471 30S ribosomal protein S11 Bacillus subtilis (strain 168) 0.013951999999999999 1 LEVO MAAARKSNTRKRRVKKNIESGIAHIRSTFNNTIVTITDTHGNAISWSSAGALGFRGSRKSTPFAAQMAAETAAKGSIEHG LKTLEVTVKGPGSGREAAIRALQAAGLEVTAIRDVTPVPHNGCRPPKRRRV P04969 30S ribosomal protein S12 Bacillus subtilis (strain 168) 0.015248736 1 LEVO MPTINQLIRKGRVSKVENSKSPALNKGYNSFKKEHTNVSSPQKRGVCTRVGTMTPKKPNSALRKYARVRLTNGIEVTAYI PGIGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGALDTAGVENRAQGRSKYGTKKPKAK P21472 30S ribosomal protein S13 Bacillus subtilis (strain 168) 0.013818084999999999 1 LEVO MARIAGVDIPRDKRVVISLTYIFGIGRTTAQQVLKEAGVSEDTRVRDLTEEELGKIRDIIDKLKVEGDLRREVSLNIKRL IEIGSYRGIRHRRGLPVRGQNSKNNARTRKGPRRTVANKKK P20282 30S ribosomal protein S14 Bacillus subtilis (strain 168) 0.007263802999999999 1 LEVO MAKKSMIAKQQRTPKFKVQEYTRCERCGRPHSVIRKFKLCRICFRELAYKGQIPGVKKASW P12878 30S ribosomal protein S15 Bacillus subtilis (strain 168) 0.010597223999999999 1 LEVO MAITQERKNQLINEFKTHESDTGSPEVQIAILTDSINNLNEHLRTHKKDHHSRRGLLKMVGKRRNLLTYLRNKDVTRYRE LINKLGLRR P21473 30S ribosomal protein S16 Bacillus subtilis (strain 168) 0.010153833 1 LEVO MAVKIRLKRMGAKKSPFYRIVVADSRSPRDGRFIETVGTYNPVAKPAEVKIDEELALKWLQTGAKPSDTVRNLFSSQGIM EKFHNAKQGK P21474 30S ribosomal protein S17 Bacillus subtilis (strain 168) 0.010220978999999998 1 LEVO MSERNQRKVYQGRVVSDKMDKTITVVVETYKKHTLYGKRVKYSKKFKAHDENNQAKIGDIVKIMETRPLSATKRFRLVEV VEEAVII P12874 30S ribosomal protein S18 Bacillus subtilis (strain 168) 0.008990613 1 LEVO MAGGRRGGRAKRRKVCYFTSNGITHIDYKDVDLLKKFVSERGKILPRRVTGTNAKYQRKLTAAIKRARQMALLPYVSGE P21475 30S ribosomal protein S19 Bacillus subtilis (strain 168) 0.010607308999999999 1 LEVO MARSLKKGPFVDGHLMTKIEKLNETDKKQVVKTWSRRSTIFPQFIGHTIAVYDGRKHVPVFISEDMVGHKLGEFAPTRTY KGHASDDKKTRR P21476 30S ribosomal protein S20 Bacillus subtilis (strain 168) 0.009622217 1 LEVO MPNIKSAIKRTKTNNERRVHNATIKSAMRTAIKQVEASVANNEADKAKTALTEAAKRIDKAVKTGLVHKNTAARYKSRLA KKVNGLSA P21477 P-tRNA Escherichia coli 0.028094644999999998 1 GCCGAGGUGGUGGAAUUGGUAGACACGCUACCUUGAGGUGGUAGUGCCCAAUAGGGCUUACGGGUUCAAGUCCCGUCCUC GGUACCA CP030939.1 TELITHROMYCIN 0.000812004 1 TEL singleParticle particle 7.8 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) 1.425 1 C1 3.1 FSC 0.143 CUT-OFF RELION 2.1 68652 PROJECTION MATCHING RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 RELION 2.1 emd_0176.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 381.96 381.96 381.96 90.0 90.0 90.0 X Y Z -0.08337495 0.1842659 0.0008619242 0.011051074 1.061 1.061 1.061 0.03 EMDB ::::EMDATABANK.org::::EMD-0176:::: None 3J9W PDB experimental model RIGID BODY FIT emd_0176_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 381.96 381.96 381.96 90.0 90.0 90.0 X Y Z -0.8643607 1.4220517 0.0014723658 0.04079013 1.061 1.061 1.061 EMDB ::::EMDATABANK.org::::EMD-0176:::: None