Complex of foot-and-mouth-disease virus (type O1 M) with the Fab of antibody D9. The virus is at 3.5 Ang resolution, the Fab is flexibly attached and at much lower resolution.

2019-11-06 REL PDBe PDBe PDBe 2018-08-02 2018-08-29 2018-08-29 2019-11-06 Medical Research Council (United Kingdom) G1000099 United Kingdom Wellcome Trust 090532/Z/09/Z United Kingdom Medical Research Council (United Kingdom) G1100525/1 United Kingdom Complex of foot-and-mouth-disease virus (type O1 M) with the Fab of antibody D9. The virus is at 3.5 Ang resolution, the Fab is flexibly attached and at much lower resolution. Shimmon G Kotecha A Ren J Asfor AS Newman J Berryman S Cottam EM Gold S Tuthill TJ King DP Brocchi E King AMQ Owens R Fry EE Stuart DI Burman A Jackson T Shimmon G Kotecha A Ren J Asfor AS Newman J Berryman S Cottam EM Gold S Tuthill TJ King DP Brocchi E King AMQ Owens R Fry EE Stuart DI Burman A Jackson T Generation and characterisation of recombinant FMDV antibodies: Applications for advancing diagnostic and laboratory assays. PLoS ONE US 13 e0201853 e0201853 2018 30114227 doi:10.1371/journal.pone.0201853 1932-6203 EMD-0173 associated EM volume Foot-and-mouth disease virus in complex with D9 FAB Foot-and-mouth disease virus in complex with D9 FAB 0 1 2 3 4 5 6 Bos taurus 9 Foot and Mouth Disease virus 300.0 3 VIRION SEROTYPE false false Foot-and-mouth disease virus 1 1 2 3 4 Foot-and-mouth disease virus Cricetinae gen. sp. D9 FAB 1 5 6 Homo sapiens Homo sapiens singleParticle particle 0.5 8.0 50.0 HEPES ETHANE 90 294 FEI VITROBOT MARK IV

FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.0 1.0 4.0 160000. 37037. GATAN 910 MULTI-SPECIMEN SINGLE TILT CRYO TRANSFER HOLDER NITROGEN 70.0 70.0 GIF Bioquantum 20 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 3838 3710 2-20 1 360 5.0 18.0 1 CTFFIND 4.0.17 1BBT I BACK PROJECTION 3.97 FSC 0.143 CUT-OFF RELION 1.3 1003 COMMON LINE RELION 1.3 COMMON LINE RELION 1.3 RELION 1.3 emd_0173.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 -399 0 0 400 400 400 540.0 540.0 540.0 90.0 90.0 90.0 X Y Z -0.10880451 0.18983106 0.0011324808 0.012099084 1.35 1.35 1.35 0.0425 AUTHOR ::::EMDATABANK.org::::EMD-0173:::: RIGID BODY FIT Cross-correlation coefficient REAL 120.0 emd_0173_additional.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 -399 0 0 400 400 400 540.0 540.0 540.0 90.0 90.0 90.0 X Y Z -0.013113795 0.029461965 0.0005899758 0.0026081277 1.35 1.35 1.35 AUTHOR ::::EMDATABANK.org::::EMD-0173:::: This is unsharpened map where the density of flexible Fabs are visible.