2020-11-04 REL PDBe PDBe PDBe 2018-07-16 2019-03-27 2019-03-27 2020-11-04 Spanish Ministry of Economy and Competitiveness BFU2015-64541-R Spain Academy of Finland 1306833, 255342,256518,283072 Finland Spanish Ministry of Economy and Competitiveness SEV-2016-0644 Spain Other government Basque Governament PRE_2016_2_0151 Spain HHIV-2 pentameric vertex complex Santos-Perez I Charro D Gil-Carton D Azkagorta M Elortza F Bamford DH Oksanen HM Abrescia NGA Santos-Perez I Charro D Gil-Carton D Azkargorta M Elortza F Bamford DH Oksanen HM Abrescia NGA Structural basis for assembly of vertical single beta-barrel viruses. Nat Commun UK 10 1184 1184 2019 30862777 doi:10.1038/s41467-019-08927-2 2041-1723 EMD-3110 other EM volume EMD-3111 other EM volume EMD-0131 associated EM volume EMD-0050 other EM volume EMD-0072 other EM volume EMD-0073 other EM volume EMD-0174 other EM volume EMD-0172 other EM volume HHIV-2 spike complex composed of VP9 and putatively of VP16, VP17 and VP2 HHIV-2 spike complex composed of VP9 and putatively of VP16, VP17 and VP2 0 Haloarcula hispanica icosahedral virus 2 singleParticle particle 1.2 7.2 20.0 C4H11NO3 Tris-HCl 20.0 MgCl2 Magnessium clorhide 10.0 CaCl2 Calcium Clorhide 0.5 NaCl Sodium Clorhide Quantifoil R2/1 COPPER 200 ETHANE
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON II (4k x 4k) INTEGRATING 35.0 1 137352
number of subboxed spike complexes extracted in silico from 2D cryo-images projections of the whole virion.
 CTFFIND 3
Reconstructed map
 1 C5 7.7 FSC 0.143 CUT-OFF RELION 2.1
the two halves were extracted randomly
58176 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 3 emd_0131.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 514.56 514.56 514.56 90.0 90.0 90.0 X Y Z -0.10049294 0.20684582 -0.0042278017 0.007181816 1.34 1.34 1.34 0.025 AUTHOR emd_0131_msk_1.map emd_0131_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 514.56 514.56 514.56 90.0 90.0 90.0 X Y Z 0 24.502857 9.129655 7.5812926 1.34 1.34 1.34 AUTHOR emd_0131_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 514.56 514.56 514.56 90.0 90.0 90.0 X Y Z -0.014110923 0.053904265 -0.0020467567 0.003149258 1.34 1.34 1.34 AUTHOR emd_0131_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 514.56 514.56 514.56 90.0 90.0 90.0 X Y Z -0.014896861 0.05369275 -0.0021810452 0.0031747066 1.34 1.34 1.34 AUTHOR