2018-11-07 REL PDBe PDBe PDBe 2018-07-13 2018-08-22 2018-11-07 2018-11-07 Ho KH Gabrielsen M Ho KL Gabrielsen M Beh PL Kueh CL Thong QX Streetley J Tan WS Bhella D PLoS Biol. US 16 e3000038 e3000038 2018 30346944 doi:10.1371/journal.pbio.3000038 1545-7885 EMD-0129 other EM volume

EMD 0129 contains a 3.3 angstrom structure of a VLP produced by recombinant expression of MrNV capsid protein

EMD-0130 associated EM volume Macrobrachium rosen Macrobrachium rosen 0

Virions were purified from Macrobrachium rosenbergii infected post-larvae

Macrobrachium rosen Macrobrachium rosenbergii Capsid 400.0 3 VIRION OTHER false false singleParticle particle 0.2 7.4

25mM HEPES, 150 mM NaCl; pH 7.4

Quantifoil R2/2 COPPER 400 CARBON CONTINUOUS GLOW DISCHARGE AIR ETHANE 100 277 FEI VITROBOT MARK IV

Virions were loaded onto Quantifoil R2/2 grids which had a continuous carbon film added. Virions were adsorbed to the carbon for 1 minute prior to blotting (4s)..

Purified virions

JEOL 2200FS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER In-column Omega Filter 20 DIRECT ELECTRON DE-20 (5k x 3k) INTEGRATING 2-40 263 2.0 60.0 1

Movies were processed to correct for movement and electron dose using Motioncor2

7236

Autopicking in Relion

RELION 2.1

Defocus estimation by GCTF

Gaussian Sphere

I 6.6 FSC 0.143 CUT-OFF RELION 2.1 3931 PROJECTION MATCHING RELION 2.1 PROJECTION MATCHING RELION 2.1 RELION 2.1