2024-05-15
REL
PDBe
PDBe
PDBe
2018-07-13
2018-08-01
2018-08-15
2024-05-15
European Molecular Biology Organization
ALTF 1008-2015
European Commission
ERC-2016-ADG 740329 EXORICO
German Research Foundation
SFB646, SFB1035, GRK1721
Louis-Jeantet Foundation
Max Planck Society
Human nuclear RNA exosome EXO-10-MPP6 complex
Gerlach P
Schuller JM
Falk S
Basquin J
Conti E
nuclear exosome, RNA decay, cryoEM, hEXO-10, hDIS3, hMPP6, RNA BINDING PROTEIN
Gerlach P
Schuller JM
Bonneau F
Basquin J
Reichelt P
Falk S
Conti E
Distinct and evolutionary conserved structural features of the human nuclear exosome complex.
Elife
US
7
2018
30047866
doi:10.7554/eLife.38686
2050-084X
EMD-0127
unknown
EMD-0127
other EM volume
Human nuclear RNA exosome EXO-14 complex
6h25
FULLOVERLAP
Human nuclear RNA exosome EXO-14 complex
Human nuclear RNA exosome EXO-14 complex
0
1
2
3
4
5
6
7
8
9
10
11
12
exosome complex
1
1
2
3
4
5
6
7
8
9
10
11
Homo sapiens
U44 ssRNA
1
12
synthetic construct
Exosome complex component RRP45
Homo sapiens
0.04937031199999999
1
Escherichia coli
LEVO
GPDSMKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGIL
FFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVA
LCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGTYLLVDPNEREERVMDGLLVIAMNKHREICTIQS
SGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEII
AEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLEDSEKEDDEGGGDQAIILDGIKMDTGVEVSDIGSQDAPIILSDSEE
EEMIILEPDKNPKKIRTQTTSAKQEKAPSKKPVKRRKKKRAAN
Q06265
Exosome complex component RRP41
Homo sapiens
0.026773328
1
Escherichia coli
LEVO
GPDSMAGLELLSDQGYRVDGRRAGELRKIQARMGVFAQADGSAYIEQGNTKALAVVYGPHEIRGSRARALPDRALVNCQY
SSATFSTGERKRRPHGDRKSCEMGLQLRQTFEAAILTQLHPRSQIDIYVQVLQADGGTYAACVNAATLAVLDAGIPMRDF
VCACSAGFVDGTALADLSHVEEAAGGPQLALALLPASGQIALLEMDARLHEDHLERVLEAAAQAARDVHTLLDRVVRQHV
REASILLGD
Q9NPD3
Exosome complex component RRP43
Homo sapiens
0.030317828
1
Escherichia coli
LEVO
RSMAAGFKTVEPLEYYRRFLKENCRPDGRELGEFRTTTVNIGSISTADGSALVKLGNTTVICGVKAEFAAPSTDAPDKGY
VVPNVDLPPLCSSRFRSGPPGEEAQVASQFIADVIENSQIIQKEDLCISPGKLVWVLYCDLICLDYDGNILDACTFALLA
ALKNVQLPEVTINEETALAEVNLKKKSYLNIRTHPVATSFAVFDDTLLIVDPTGEEEHLATGTLTIVMDEEGKLCCLHKP
GGSGLTGAKLQDCMSRAVTRHKEVKKLMDEVIKSMKPK
Q96B26
Exosome complex component RRP46
Homo sapiens
0.02552425
1
Escherichia coli
LEVO
RSMEEETHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEV
ILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACA
LDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS
Q9NQT4
Exosome complex component RRP42
Homo sapiens
0.032216761999999996
1
Escherichia coli
LEVO
GPDSMASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPKLEKPNE
GYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRIFNNKSSVDLKTLCISPREHCWVLYVDVLLLECGGNLFDAISIAV
KAALFNTRIPRVRVLEDEEGSKDIELSDDPYDCIRLSVENVPCIVTLCKIGYRHVVDATLQEEACSLASLLVSVTSKGVV
TCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVVHKEESLGPKRQKVGFLG
Q15024
Exosome complex component MTR3
Homo sapiens
0.028623469
1
Escherichia coli
LEVO
GPDSMPGDHRRIRGPEESQPPQLYAADEEEAPGTRDPTRLRPVYARAGLLSQAKGSAYLEAGGTKVLCAVSGPRQAEGGE
RGGGPAGAGGEAPAALRGRLLCDFRRAPFAGRRRRAPPGGCEERELALALQEALEPAVRLGRYPRAQLEVSALLLEDGGS
ALAAALTAAALALADAGVEMYDLVVGCGLSLAPGPAPTWLLDPTRLEEERAAAGLTVALMPVLNQVAGLLGSGEGGLTES
WAEAVRLGLEGCQRLYPVLQQSLVRAARRRGAAAQP
Q5RKV6
Exosome complex component RRP40
Homo sapiens
0.030906358999999998
1
Escherichia coli
LEVO
GSGSGSGSGSGSGSGSGSMAEPASVAAESLAGSRARAARTVLGQVVLPGEELLLPEQEDAEGPGGAVERPLSLNARACSR
VRVVCGPGLRRCGDRLLVTKCGRLRHKEPGSGSGGGVYWVDSQQKRYVPVKGDHVIGIVTAKSGDIFKVDVGGSEPASLS
YLSFEGATKRNRPNVQVGDLIYGQFVVANKDMEPEMVCIDSCGRANGMGVIGQDGLLFKVTLGLIRKLLAPDCEIIQEVG
KLYPLEIVFGMNGRIWVKAKTIQQTLILANILEACEHMTSDQRKQIFSRLAES
Q9NQT5
Exosome complex component RRP4
Homo sapiens
0.033190355000000005
1
Escherichia coli
LEVO
GPDSMAMEMRLPVARKPLSERLGRDTKKHLVVPGDTITTDTGFMRGHGTYMGEEKLIASVAGSVERVNKLICVKALKTRY
IGEVGDIVVGRITEVQQKRWKVETNSRLDSVLLLSSMNLPGGELRRRSAEDELAMRGFLQEGDLISAEVQAVFSDGAVSL
HTRSLKYGKLGQGVLVQVSPSLVKRQKTHFHDLPCGASVILGNNGFIWIYPTPEHKEEEAGGFIANLEPVSLADREVISR
LRNCIISLVTQRMMLYDTSILYCYEASLPHQIKDILKPEIMEEIVMETRQRLLEQEG
Q13868
Exosome complex component CSL4
Homo sapiens
0.021835099999999996
1
Escherichia coli
LEVO
GPDSMAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVS
SINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVA
HSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT
Q9Y3B2
Exosome complex exonuclease RRP44
Homo sapiens
0.109522227
1
Escherichia coli
LEVO
GPDSMLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDTNVLLHQ
IDVLEDPAIRNVIVLQTVLQEVRNRSAPVYKRIRDVTNNQEKHFYTFTNEHHRETYVEQEQGENANDRNNRAIRVAAKWY
NEHLKKMSADNQLQVIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDRLACLSEEGNEIESGKIIFSEHLPLSK
LQQGIKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLPKSQWVAPSSVVLHDEGQN
EEDVEKEEETERMLKTAVSEKMLKPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASTLEGR
RIIVAIDGWPRNSRYPNGHFVRNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEKDMKNREDLRHLCIC
SVDPPGCTDINDALHCRELENGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVD
RLAFSCIWEMNHNAEILKTKFTKSVINSKASLTYAEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPE
VRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTD
TAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIG
ADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFE
EKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKL
GK
Q9Y2L1
3.1.13.-
M-phase phosphoprotein 6
Homo sapiens
0.019542234
1
Escherichia coli
LEVO
GPDSASMAAERKTKLSKNLLRMKFMQRGLDSETKKQLEEEEKKIISEEHWYLDLPELKEKESFIIEEQSFLLCEDLLYGR
MSFRGFNPEVEKLMLQMNAKHKAEEVEDETVELDVSDEEMARRYETLVGTIGKKFARKRDHANYEEDENGDITPIKAKKM
FLKPQD
Q99547
U44 ssRNA
synthetic construct
0.013426333
1
UUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUU
singleParticle
particle
0.3
7.5
20.0
Hepes
150.0
NaCl
2.0
DTT
ETHANE-PROPANE
FEI VITROBOT MARK IV
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
GATAN K2 SUMMIT (4k x 4k)
COUNTING
8047
47.0
1
2396236
3.8
FSC 0.143 CUT-OFF
RELION
2.1
110958
PROJECTION MATCHING
PROJECTION MATCHING