2024-05-15 REL PDBe PDBe PDBe 2018-07-05 2018-08-22 2018-10-24 2024-05-15 Novo Nordisk Foundation NNF12OC1015894 Denmark Flygaard RK Jenner LB Ribosome, hibernation, 100S, dimer, cryo-EM Flygaard RK Boegholm N Yusupov M Jenner LB Nat Commun UK 9 4179 4179 2018 30301898 doi:10.1038/s41467-018-06724-x 2041-1723 EMD-0101 other EM volume

T. thermophilus hibernating 70S ribosome from 100S dimer

EMD-0104 other EM volume

T. thermophilus hibernating 100S ribosome (ice)

6gzz FULLOVERLAP Thermus thermophilus hibernating 100S ribosome (amc) Thermus thermophilus hibernating 100S ribosome (amc) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54

T. thermophilus hibernating 100S ribosome cryo-EM structure from grids coated with continuous amorphous carbon

2.35 100S ribosome 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) Ribosome hibernation promoting factor 1 52 54 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 50S ribosomal protein L2 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.029985798999999997 2 LEVO AVKKFKPYTPSRRFMTVADFSEITKTEPEKSLVKPLKKTGGRNNQGRITVRFRGGGHKRLYRIIDFKRWDKVGIPAKVAA IEYDPNRSARIALLHYVDGEKRYIIAPDGLQVGQQVVAGPDAPIQVGNALPLRFIPVGTVVHAVELEPKKGAKLARAAGT SAQIQGREGDYVILRLPSGELRKVHGECYATVGAVGNADHKNIVLGKAGRSRWLGRRPHVRGAAMNPVDHPHGGGEGRAP RGRPPASPWGWQTKGLKTRKRRKPSSRFIIAR P60405 50S ribosomal protein L3 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.022321030999999998 2 LEVO MKGILGVKVGMTRIFRDDRAVPVTVILAGPCPVVQRRTPEKDGYTAVQLGFLPQNPKRVNRPLKGHFAKAGVEPVRILRE IRDFNPEGDTVTVEIFKPGERVDVTGTSKGRGFAGVMKRWNFAGGPDSHGAHKIHRHPGSIGNRKTPGRVYKGKKMAGHY GAERVTVMNLEVVDVIPEENLLLVKGAVPGPNGGLVIVRETKKAA Q5SHN8 50S ribosomal protein L4 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.023071721 2 LEVO MKEVAVYQIPVLSPSGRRELAADLPAEINPHLLWEVVRWQLAKRRRGTASTKTRGEVAYSGRKIWPQKHTGRARHGDIGA PIFVGGGVVFGPKPRDYSYTLPKKVRKKGLAMAVADRAREGKLLLVEAFAGVNGKTKEFLAWAKEAGLDGSESVLLVTGN ELVRRAARNLPWVVTLAPEGLNVYDIVRTERLVMDLDAWEVFQNRIGG Q5SHN9 50S ribosomal protein L5 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.020930400000000002 2 LEVO PLDVALKRKYYEEVRPELIRRFGYQNVWEVPRLEKVVINQGLGEAKEDARILEKAAQELALITGQKPAVTRAKKSISNFK LRKGMPIGLRVTLRRDRMWIFLEKLLNVALPRIRDFRGLNPNSFDGRGNYNLGLREQLIFPEITYDMVDALRGMDIAVVT TAETDEEARALLELLGFPFRK Q5SHQ0 50S ribosomal protein L6 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.018567633 2 LEVO SRIGRLPIPVPKGVSVEVAPGRVKVKGPKGELEVPVSPEMRVVVEEGVVRVERPSDERRHKSLHGLTRTLIANAVKGVSE GYSKELLIKGIGYRARLVGRALELTVGFSHPVVVEPPEGITFEVPEPTRVRVSGIDKQKVGQVAANIRAIRKPSAYHEKG IYYAGEPVRL P0DOY8 50S ribosomal protein L9 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.005454387999999999 2 LEVO MKVILLEPLENLGDVGQVVDVKPGYARNYLLPRGLAVLATESNLKALEAR Q5SLQ1 50S ribosomal protein L13 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.015699658 2 LEVO MKTYVPKQVEPRWVLIDAEGKTLGRLATKIATLLRGKHRPDWTPNVAMGDFVVVVNADKIRVTGKKLEQKIYTRYSGYPG GLKKIPLEKMLATHPERVLEHAVKGMLPKGPLGRRLFKRLKVYAGPDHPHQAQRPEKL P60488 50S ribosomal protein L14 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.013323611999999999 2 LEVO MIQPQTYLEVADNTGARKIMCIRVLKGSNAKYATVGDVIVASVKEAIPRGAVKEGDVVKAVVVRTKKEIKRPDGSAIRFD DNAAVIINNQLEPRGTRVFGPVARELREKGFMKIVSLAPEVL Q5SHP8 50S ribosomal protein L15 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.016319142 2 LEVO MKLSDLRPNPGANKRRKRVGRGPGSGHGKTATRGHKGQKSRSGGLKDPRRFEGGRSTTLMRLPKRGMQGQVPGEIKRPRY QGVNLKDLARFEGEVTPELLVRAGLLKKGYRLKILGEGEAKPLKVVAHAFSKSALEKLKAAGGEPVLLEA Q5SHQ7 50S ribosomal protein L16 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.015993909 2 LEVO MLMPRRMKYRKQQRGRLKGATKGGDYVAFGDYGLVALEPAWITAQQIEAARVAMVRHFRRGGKIFIRIFPDKPYTKKPLE VRMGKGKGNVEGYVAVVKPGRVMFEVAGVTEEQAMEALRIAGHKLPIKTKIVRRDAYDEAQ P60489 50S ribosomal protein L17 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.013618951999999998 2 LEVO RHLKSGRKLNRHSSHRLALYRNQAKSLLTHGRITTTVPKAKELRGFVDHLIHLAKRGDLHARRLVLRDLQDVKLVRKLFD EIAPRYRDRQGGYTRVLKLAERRRGDGAPLALVELVE Q9Z9H5 50S ribosomal protein L18 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.012508648999999998 2 LEVO ARLTAYERRKFRVRNRIKRTGRLRLSVFRSLKHIYAQIIDDEKGVTLVSASSLALKLKGNKTEVARQVGRALAEKALALG IKQVAFDRGPYKYHGRVKALAEGAREGGLEF Q5SHQ4 50S ribosomal protein L19 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.016218812 2 LEVO MNRGALIKLVESRYVRTDLPEFRPGDTVRVSYKVKEGNRTRIQDFEGIVIRIRRNGFNTTFTVRKVSYGVGVERIFPLHS PLIQKIDIVQRGRARRAKLYFIRNLSDREIRRKLRADRKRIDQDRAAERAAKEEAQK P60490 50S ribosomal protein L20 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.013648081 2 LEVO PRAKTGVVRRRKHKKILKLAKGYWGLRSKSFRKARETLFAAGNYAYAHRKRRKRDFRRLWIVRINAACRQHGLNYSTFIH GLKKAGIEVDRKNLADLAVREPQVFAELVERAKAAQG P60491 50S ribosomal protein L21 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.011069153 2 LEVO MFAIVKTGGKQYRVEPGLKLRVEKLDAEPGATVELPVLLLGGEKTVVGTPVVEGASVVAEVLGHGRGKKILVSKFKAKVQ YRRKKGHRQPYTELLIKEIRG P60492 50S ribosomal protein L22 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.012808055 2 LEVO MEAKAIARYVRISPRKVRLVVDLIRGKSLEEARNILRYTNKRGAYFVAKVLESAAANAVNNHDMLEDRLYVKAAYVDEGP ALKRVLPRARGRADIIKKRTSHITVILGEKHGK Q5SHP3 50S ribosomal protein L23 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.010273117 2 LEVO TAYDVILAPVLSEKAYAGFAEGKYTFWVHPKATKTEIKNAVETAFKVKVVKVNTLHVRGKKKRLGRYLGKRPDRKKAIVQ VAPGQKIEALEG Q5SHP0 50S ribosomal protein L24 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.011238709999999999 2 LEVO RVKMHVKKGDTVLVASGKYKGRVGKVKEVLPKKYAVIVEGVNIVKKAVRVSPKYPQGGFIEKEAPLHASKVRPICPACGK PTRVRKKFLENGKKIRVCAKCG Q5SHP9 50S ribosomal protein L25 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.02026833 2 LEVO MEYRLKAYYREGEKPSALRRAGKLPGVMYNRHLNRKVYVDLVEFDKVFRQASIHHVIVLELPDGQSLPTLVRQVNLDKRR RRPEHVDFFVLSDEPVEMYVPLRFVGTPAGVRAGGVLQEIHRDILVKVSPRNIPEFIEVDVSGLEIGDSLHASDLKLPPG VELAVSPEETIAAVVPPED Q5SHZ1 50S ribosomal protein L27 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.008703032 2 LEVO STRNGRDSQAKRLGVKRYEGQVVRAGNILVRQRGTRFKPGKNVGMGRDFTLFALVDGVVEFQDRGRLGRYVHVRPLA P60493 50S ribosomal protein L28 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.010872965 2 LEVO SKVCEISGKRPIVANSIQRRGKAKREGGVGKKTTGISKRRQYPNLQKVRVRVAGQEITFRVAASHIPKVYELVERAKGLK LEGLSPKEIKKELLKLL P60494 50S ribosomal protein L29 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.008296725 2 LEVO SEVRKQLEEARKLSPVELEKLVREKKRELMELRFQASIGQLSQNHKIRDLKRQIARLLTVLNEKRRQNA Q5SHP6 50S ribosomal protein L30 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.006667930999999999 2 LEVO PRLKVKLVKSPIGYPKDQKAALKALGLRRLQQERVLEDTPAIRGNVEKVAHLVRVEVVE Q5SHQ6 50S ribosomal protein L31 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.007377532 2 LEVO MKEGIHPKLVPARIICGCGNVIETYSTKPEIYVEVCSKCHPFYTGQQRFVDTEGRVERFQRRY Q5SJE1 50S ribosomal protein L32 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.006590854 2 LEVO AKHPVPKKKTSKARRDARRSHHALTPPTLVPCPECKAMKPPHTVCPECGYYAGRKVLEV P80339 50S ribosomal protein L33 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.005602612 2 LEVO LLLECTECKRRNYATEKNKRNTPNKLELRKYCPWCRKHTVHREVK P35871 50S ribosomal protein L34 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.006132448999999999 2 LEVO MKRTWQPNRRKRAKTHGFRARMRTPGGRKVLKRRRQKGRWRLTPAVRKR P80340 50S ribosomal protein L35 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.006985579 2 LEVO PKMKTHKGAKKRVKITASGKVVAMKTGKRHLNWQKSGKEIRQKGRKFVLAKPEAERIKLLL Q5SKU1 23S ribosomal RNA Thermus thermophilus HB8 0.947013 2 GGUCAAGAUGGUAAGGGCCCACGGUGGAUGCCUCGGCACCCGAGCCGAUGAAGGACGUGGCUACCUGCGAUAAGCCAGGG GGAGCCGGUAGCGGGCGUGGAUCCCUGGAUGUCCGAAUGGGGGAACCCGGCCGGCGGGAACGCCGGUCACCGCGCUUUUU GCGCGGGGGGAACCUGGGGAACUGAAACAUCUCAGUACCCAGAGGAGAGGAAAGAGAAAUCGACUCCCUGAGUAGCGGCG AGCGAAAGGGGACCAGCCUAAACCGUCCGGCUUGUCCGGGCGGGGUCGUGGGGCCCUCGGACACCGAAUCCCCAGCCUAG CCGAAGCUGUUGGGAAGCAGCGCCAGAGAGGGUGAAAGCCCCGUAGGCGAAAGGUGGGGGGAUAGGUGAGGGUACCCGAG UACCCCGUGGUUCGUGGAGCCAUGGGGGAAUCUGGGCGGACCACCGCCUAAGGCUAAGUACUCCGGGUGACCGAUAGCGC ACCAGUACCGUGAGGGAAAGGUGAAAAGAACCCCGGGAGGGGAGUGAAAUAGAGCCUGAAACCGUGGGCUUACAAGCAGU CACGGCCCCGCAAGGGGUUGUGGCGUGCCUAUUGAAGCAUGAGCCGGCGACUCACGGUCGUGGGCGAGCUUAAGCCGUUG AGGCGGAGGCGUAGGGAAACCGAGUCCGAACAGGGCGCAAACGGCCCGCACGCGGGCCGAAAAGUCCGCGGCCGUGGACC CGAAACCGGGCGAGCUAGCCCUGGCCAGGGUGAAGCUGGGGUGAGACCCAGUGGAGGCCCGAACCGGUGGGGGAUGCAAA CCCCUCGGAUGAGCUGGGGCUAGGAGUGAAAAGCUAACCGAGCCCGGAGAUAGCUGGUUCUCCCCGAAAUGACUUUAGGG UCAGCCUCAGGCGCUGACUGGGGCCUGUAGAGCACUGAUAGGGCUAGGGGGCCCACCAGCCUACCAAACCCUGUCAAACU CCGAAGGGUCCCAGGUGGAGCCUGGGAGUGAGGGCGCGAGCGAUAACGUCCGCGUCCGAGCGCGGGAACAACCGAGACCG CCAGCUAAGGCCCCCAAGUCUGGGCUAAGUGGUAAAGGAUGUGGCGCCGCGAAGACAGCCAGGAUGUUGGCUUAGAAGCA GCCAUCAUUUAAAGAGUGCGUAAUAGCUCACUGGUCGAGUGGCGCCGCGCCGAAAAUGAUCGGGGCUUAAGCCCAGCGCC GAAGCUGCGGGUCUGGGGGAUGACCCCAGGCGGUAGGGGAGCGUUCCCGAUGCCGAUGAAGGCCGACCCGCGAGGGCGGC UGGAGGUAAGGGAAGUGCGAAUGCCGGCAUGAGUAACGAUAAAGAGGGUGAGAAUCCCUCUCGCCGUAAGCCCAAGGGUU CCUACGCAAUGGUCGUCAGCGUAGGGUUAGGCGGGACCUAAGGUGAAGCCGAAAGGCGUAGCCGAAGGGCAGCCGGUUAA UAUUCCGGCCCUUCCCGCAGGUGCGAUGGGGGGACGCUCUAGGCUAGGGGGACCGGAGCCAUGGACGAGCCCGGCCAGAA GCGCAGGGUGGGAGGUAGGCAAAUCCGCCUCCCAACAAGCUCUGCGUGGUGGGGAAGCCCGUACGGGUGACAACCCCCCG AAGCCAGGGAGCCAAGAAAAGCCUCUAAGCACAACCUGCGGGAACCCGUACCGCAAACCGACACAGGUGGGCGGGUGCAA GAGCACUCAGGCGCGCGGGAGAACCCUCGCCAAGGAACUCUGCAAGUUGGCCCCGUAACUUCGGGAGAAGGGGUGCUCCC UGGGGUGAUGAGCCCCGGGGAGCCGCAGUGAACAGGCUCUGGCGACUGUUUACCAAAAACACAGCUCUCUGCGAACUCGU AAGAGGAGGUAUAGGGAGCGACGCUUGCCCGGUGCCGGAAGGUCAAGGGGAGGGGUGCAAGCCCCGAACCGAAGCCCCGG UGAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAAAGCGUA ACGACCGGAGCGCUGUCUCGGCGAGGGACCCGGUGAAAUUGAACUGGCCGUGAAGAUGCGGCCUACCCGUGGCAGGACGA AAAGACCCCGUGGAGCUUUACUGCAGCCUGGUGUUGGCUCUUGGUCGCGCCUGCGUAGGAUAGGUGGGAGCCUGUGAACC CCCGCCUCCGGGUGGGGGGGAGGCGCCGGUGAAAUACCACCCUGGCGCGGCUGGGGGCCUAACCCUCGGAUGGGGGGACA GCGCUUGGCGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGUCCCCUCAGGCGGGACG GAAAUCCGCCGGAGAGCGCAAGGGUAGAAGGGGGCCUGACUGCGAGGCCUGCAAGCCGAGCAGGGGCGAAAGCCGGGCCU AGUGAACCGGUGGUCCCGUGUGGAAGGGCCAUCGAUCAACGGAUAAAAGUUACCCCGGGGAUAACAGGCUGAUCUCCCCC GAGCGUCCACAGCGGCGGGGAGGUUUGGCACCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGAAGAAGGUCCCAAGGGUU GGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCUCUAUCCGCCACGGGCG CAGGAGGCUUGAGGGGGGCUCUUCCUAGUACGAGAGGACCGGAAGGGACGCACCUCUGGUUUCCCAGCUGUCCCUCCAGG GGCAUAAGCUGGGUAGCCAUGUGCGGAAGGGAUAACCGCUGAAAGCAUCUAAGCGGGAAGCCCGCCCCAAGAUGAGGCCU CCCACGGCGUCAAGCCGGUAAGGACCCGGGAAGACCACCCGGUGGAUGGGCCGGGGGUGUAAGCGCCGCGAGGCGUUGAG CCGACCGGUCCCAAUCGUCCGAGGUCUUGACC AP008226.1 5S ribosomal RNA Thermus thermophilus HB8 0.03954061699999999 2 AAUCCCCCGUGCCCAUAGCGGCGUGGAACCACCCGUUCCCAUUCCGAACACGGAAGUGAAACGCGCCAGCGCCGAUGGUA CUGGGCGGGCGACCGCCUGGGAGAGUAGGUCGGUGCGGGGGA AP008226.1 50S ribosomal protein L36 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.004304215 2 LEVO KVRASVKRICDKCKVIRRHGRVYVICENPKHKQRQG Q5SHR2 30S ribosomal protein S2 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.027330648 2 LEVO EITVKELLEAGVHFGHERKRWNPKFARYIYAERNGIHIIDLQKTMEELERTFRFIEDLAMRGGTILFVGTKKQAQDIVRM EAERAGMPYVNQRWLGGMLTNFKTISQRVHRLEELEALFASPEIEERPKKEQVRLKHELERLQKYLSGFRLLKRLPDAIF VVDPTKEAIAVREARKLFIPVIALADTDSDPDLVDYIIPGNDDAIRSIQLILSRAVDLIIQARGGVVEPSPSYALVQ P80371 30S ribosomal protein S3 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.02286243 2 LEVO GNKIHPIGFRLGITRDWESRWYAGKKQYRHLLLEDQRIRGLLEKELYSAGLARVDIERAADNVAVTVHVAKPGVVIGRGG ERIRVLREELAKLTGKNVALNVQEVQNPNLSAPLVAQRVAEQIERRFAVRRAIKQAVQRVMESGAKGAKVIVSGRIGGAE QARTEWAAQGRVPLHTLRANIDYGFALARTTYGVLGVKAYIFLGEV P80372 30S ribosomal protein S4 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.024242254 2 LEVO GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRPSDYAVRLREKQKLRRIYGISERQFRNLFEE ASKKKGVTGSVFLGLLESRLDNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQN LEAMKGRKVGPWLSLDVEGMKGKFLRLPDREDLALPVNEQLVIEFYSR P80373 30S ribosomal protein S5 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.016460192999999998 2 LEVO DFEEKMILIRRTARMQAGGRRFRFGALVVVGDRQGRVGLGFGKAPEVPLAVQKAGYYARRNMVEVPLQNGTIPHEIEVEF GASKIVLKPAAPGTGVIAGAVPRAILELAGVTDILTKELGSRNPINIAYATMEALRQLRTKADVERLRKGE Q5SHQ5 30S ribosomal protein S6 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.011988753 2 LEVO MRRYEVNIVLNPNLDQSQLALEKEIIQRALENYGARVEKVEELGLRRLAYPIAKDPQGYFLWYQVEMPEDRVNDLARELR IRDNVRRVMVVKSQEPFLANA Q5SLP8 30S ribosomal protein S7 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.017919775000000002 2 LEVO ARRRRAEVRQLQPDLVYGDVLVTAFINKIMRDGKKNLAARIFYDACKIIQEKTGQEPLKVFKQAVENVKPRMEVRSRRVG GANYQVPMEVSPRRQQSLALRWLVQAANQRPERRAAVRIAHELMDAAEGKGGAVKKKEDVERMAEANRAYAHYRW P17291 30S ribosomal protein S8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.01586857 2 LEVO MLTDPIADMLTRIRNATRVYKESTDVPASRFKEEILRILAREGFIKGYERVDVDGKPYLRVYLKYGPRRQGPDPRPEQVI HHIRRISKPGRRVYVGVKEIPRVRRGLGIAILSTSKGVLTDREARKLGVGGELICEVW P0DOY9 30S ribosomal protein S9 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.014279419 2 LEVO EQYYGTGRRKEAVARVFLRPGNGKVTVNGQDFNEYFQGLVRAVAALEPLRAVDALGHFDAYITVRGGGKSGQIDAIKLGI ARALVQYNPDYRAKLKPLGFLTRDARVVERKKYGKHKARRAPQYSKR P80374 30S ribosomal protein S10 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.011398308000000001 2 LEVO KIRIKLRGFDHKTLDASAQKIVEAARRSGAQVSGPIPLPTRVRRFTVIRGPFKHKDSREHFELRTHNRLVDIINPNRKTI EQLMTLDLPTGVEIEIKTV Q5SHN7 30S ribosomal protein S11 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.012519292 2 LEVO KRQVASGRAYIHASYNNTIVTITDPDGNPITWSSGGVIGYKGSRKGTPYAAQLAALDAAKKAMAYGMQSVDVIVRGTGAG REQAIRALQASGLQVKSIVDDTPVPHNGCRPKKKFRKA P80376 30S ribosomal protein S12 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.013875388 2 LEVO PTINQLVRKGREKVRKKSKVPALKGAPFRRGVCTVVRTVTPKKPNSALRKVAKVRLTSGYEVTAYIPGEGHNLQEHSVVL IRGGRVKDLPGVRYHIVRGVYDAAGVKDRKKSRSKYGTKKPKEAA Q5SHN3 30S ribosomal protein S13 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.013308507999999998 2 LEVO ARIAGVEIPRNKRVDVALTYIYGIGKARAKEALEKTGINPATRVKDLTEAEVVRLREYVENTWKLEGELRAEVAANIKRL MDIGCYRGLRHRRGLPVRGQRTRTNARTRKGPRKTVA P80377 30S ribosomal protein S14 type Z Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.007027529 2 LEVO ARKALIEKAKRTPKFKVRAYTRCVRCGRARSVYRFFGLCRICLRELAHKGQLPGVRKASW P0DOY6 30S ribosomal protein S15 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.010447212999999999 2 LEVO PITKEEKQKVIQEFARFPGDTGSTEVQVALLTLRINRLSEHLKVHKKDHHSHRGLLMMVGQRRRLLRYLQREDPERYRAL IEKLGIRG Q5SJ76 30S ribosomal protein S16 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.009995546 2 LEVO MVKIRLARFGSKHNPHYRIVVTDARRKRDGKYIEKIGYYDPRKTTPDWLKVDVERARYWLSVGAQPTDTARRLLRQAGVF RQEA Q5SJH3 30S ribosomal protein S17 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.011880097999999999 2 LEVO PKKVLTGVVVSDKMQKTVTVLVERQFPHPLYGKVIKRSKKYLAHDPEEKYKLGDVVEIIESRPISKRKRFRVLRLVESGR MDLVEKYLIRRQNYESLSKR P0DOY7 30S ribosomal protein S18 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.007312722999999999 2 LEVO GEFDLRDYRNVEVLKRFLSETGKILPRRRTGLSAKEQRILAKTIKRARILGLLPFTEKLVRK Q5SLQ0 30S ribosomal protein S19 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.008872271000000001 2 LEVO KGVFVDDHLLEKVLELNAKGEKRLIKTWSRRSTIVPEMVGHTIAVYNGKQHVPVYITENMVGHKLGEFAPTRTYRGHG Q5SHP2 30S ribosomal protein S20 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.010921085999999998 2 LEVO RNLSALKRHRQSLKRRLRNKAKKSAIKTLSKKAIQLAQEGKAEEALKIMRKAESLIDKAAKGSTLHKNAAARRKSRLMRK VRQLLEAAGAPLIGGGLSA P80380 30S ribosomal protein Thx Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.003089655 2 LEVO GKGDRRTRRGKIWRGTYGKYRPRKK Q5SIH3 Ribosome hibernation promoting factor Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.0067698079999999996 2 Escherichia coli LEVO PRIVRVKRFEMKPMDPEEAAFQMEALGHSFFVFRNAKTDEINVIYRRKDGNYGLIEP Q5SIS0 16S ribosomal RNA Thermus thermophilus HB8 0.489002531 2 UGGAGAGUUUGAUCCUGGCUCAGGGUGAACGCUGGCGGCGUGCCUAAGACAUGCAAGUCGUGCGGGCCGCGGGGUUUUAC UCCGUGGUCAGCGGCGGACGGGUGAGUAACGCGUGGGUGACCUACCCGGAAGAGGGGGACAACCCGGGGAAACUCGGGCU AAUCCCCCAUGUGGACCCGCCCCUUGGGGUGUGUCCAAAGGGCUUUGCCCGCUUCCGGAUGGGCCCGCGUCCCAUCAGCU AGUUGGUGGGGUAAUGGCCCACCAAGGCGACGACGGGUAGCCGGUCUGAGAGGAUGGCCGGCCACAGGGGCACUGAGACA CGGGCCCCACUCCUACGGGAGGCAGCAGUUAGGAAUCUUCCGCAAUGGGCGCAAGCCUGACGGAGCGACGCCGCUUGGAG GAAGAAGCCCUUCGGGGUGUAAACUCCUGAACCCGGGACGAAACCCCCGACGAGGGGACUGACGGUACCGGGGUAAUAGC GCCGGCCAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGCGCGAGCGUUACCCGGAUUCACUGGGCGUAAAGGGCGUG UAGGCGGCCUGGGGCGUCCCAUGUGAAAGACCACGGCUCAACCGUGGGGGAGCGUGGGAUACGCUCAGGCUAGACGGUGG GAGAGGGUGGUGGAAUUCCCGGAGUAGCGGUGAAAUGCGCAGAUACCGGGAGGAACGCCGAUGGCGAAGGCAGCCACCUG GUCCACCCGUGACGCUGAGGCGCGAAAGCGUGGGGAGCAAACCGGAUUAGAUACCCGGGUAGUCCACGCCCUAAACGAUG CGCGCUAGGUCUCUGGGUCUCCUGGGGGCCGAAGCUAACGCGUUAAGCGCGCCGCCUGGGGAGUACGGCCGCAAGGCUGA AACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAAGCAACGCGAAGAACCUUACCAG GCCUUGACAUGCUAGGGAACCCGGGUGAAAGCCUGGGGUGCCCCGCGAGGGGAGCCCUAGCACAGGUGCUGCAUGGCCGU CGUCAGCUCGUGCCGUGAGGUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCCCGCCGUUAGUUGCCAGCGGUUCGGCCG GGCACUCUAACGGGACUGCCCGCGAAAGCGGGAGGAAGGAGGGGACGACGUCUGGUCAGCAUGGCCCUUACGGCCUGGGC GACACACGUGCUACAAUGCCCACUACAAAGCGAUGCCACCCGGCAACGGGGAGCUAAUCGCAAAAAGGUGGGCCCAGUUC GGAUUGGGGUCUGCAACCCGACCCCAUGAAGCCGGAAUCGCUAGUAAUCGCGGAUCAGCCAUGCCGCGGUGAAUACGUUC CCGGGCCUUGUACACACCGCCCGUCACGCCAUGGGAGCGGGCUCUACCCGAAGUCGCCGGGAGCCUACGGGCAGGCGCCG AGGGUAGGGCCCGUGACUGGGGCGAAGUCGUAACAAGGUAGCUGUACCGGAAGGUGCGGCUGGAUC AP008226.1 Ribosome hibernation promoting factor Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 0.013694619 2 Escherichia coli LEVO NIYKLIGRNLEITDAIRDYVEKKLARLDRYQDGELMAKVVLSLAGSPHVEKKARAEIQVDLPGGLVRVEEEDADLYAAID RAVDRLETQVKRFRERRYVGKRHSYQGPPPPEVRDLEAL Q5SIS0 singleParticle particle 7.5 ETHANE FEI VITROBOT MARK IV FEI TITAN KRIOS OTHER OTHER FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 1.06 1 4.13 FSC 0.143 CUT-OFF 12126 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD