2024-10-23 REL PDBe PDBe PDBe 2018-06-20 2018-08-22 2019-07-03 2024-10-23 Biotechnology and Biological Sciences Research Council BB/J014575/1 United Kingdom GAPDH-CP12-PRK complex McFarlane CR Shah N complex, calvin cycle, redox regulation, PHOTOSYNTHESIS McFarlane CR Shah NR Kabasakal BV Echeverria B Cotton CAR Bubeck D Murray JW Structural basis of light-induced redox regulation in the Calvin-Benson cycle in cyanobacteria. Proc.Natl.Acad.Sci.USA US 116 20984 20990 2019 31570616 doi:10.1073/pnas.1906722116 1091-6490 0040 PNASA6 6gve FULLOVERLAP GAPDH-CP12-PRK complex GAPDH-CP12-PRK complex 0 1 2 3 0.480 GAPDH-CP12-PRK complex 1 1 Thermosynechococcus elongatus BP-1 GAPDH-CP12-PRK complex 1 2 3 Thermosynechococcus elongatus BP-1 CP12 polypeptide Thermosynechococcus elongatus (strain BP-1) BP-1 0.008611127 4 Escherichia coli LEVO GSMSNLEKQIEQAREEAHKICDTEGATSGQCAAAWDALEELQAEAAHQRAEQQDHKTSFQQYCDDNPDAAECRIYDD Q8DHX3 Glyceraldehyde-3-phosphate dehydrogenase Thermosynechococcus elongatus (strain BP-1) BP-1 0.036792733999999994 8 Escherichia coli LEVO GSMVRVAINGFGRIGRNFMRCWLQRKANSKLEIVGINDTSDPRTNAHLLKYDSMLGIFQDAEITADDDCIYAGGHAVKCV SDRNPENLPWSAWGIDLVIEATGVFTSREGASKHLSAGAKKVLITAPGKGNIPTYVVGVNHHTYDPSEDIVSNASCTTNC LAPIVKVLHEAFGIQQGMMTTTHSYTGDQRLLDASHRDLRRARAAAMNIVPTSTGAAKAVGLVIPELQGKLNGIALRVPT PNVSVVDFVAQVEKPTIAEQVNQVIKEASETTMKGIIHYSELELVSSDYRGHNASSILDASLTMVLGGNLVKVVAWYDNE WGYSQRVLDLAEHMAAHWA Q8DIW5 1.2.1.- Phosphoribulokinase Thermosynechococcus elongatus (strain BP-1) BP-1 0.038038234 4 LEVO MSSKPDRVVLIGVAGDSGCGKSTFLRRLADLFGEDFMTVICLDDYHSLDRKQRKEMGITALDPRANNFDLMYEQIKALKN GESIMKPIYNHETGTIDPPEKVDPNHVIVIEGLHPLYDERVRSLIDFSVYLDISDDVKIAWKIKRDMAERGHSYEDVIAS INARRPDFMAYIDPQKQYADVVLQILPSQLAKEEKVGNILRVRMLQREGIPGFEPVYLFDEGSTITWIPCGRKLTCSYPG IRLSYGPDEYYGHPVSVLEVDGRFEKLDELIYIESHLSNTSTKHYGEVTELLLKHRDYPGSDNGSGLFQVLTGLKMRATY ERLTSRDAATVTNR Q8DHN2 2.7.1.19 NICOTINAMIDE-ADENINE-DINUCLEOTIDE 0.0006634249999999999 8 NAD singleParticle particle 7.9 1.0 C21H27N7O14P2 Nicotinamide adenine dinucleotide 1.0 C10H15N5O10P2 Adenosine diphosphate 1.0 C4H8O2S2 trans-4,5-Dihydroxy-1,2-dithiane 50.0 NaCl soidium chloride 10.0 MgCl2 magnesium chloride 50.0 C4H11NO3 Tris-HCl Quantifoil R2/2 COPPER 300 CARBON CONTINUOUS 3.0 GLOW DISCHARGE AIR ETHANE 85 283 FEI VITROBOT MARK IV
Blot force 3. Wait time 60 seconds, then blotted for 3.5 seconds before plunging. 2.5 ul of sample..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 44.0 1 Generated in Relion by the Initial Model generation tool D2 3.9 FSC 0.143 CUT-OFF 197212 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0071.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 366.45 366.45 366.45 90.0 90.0 90.0 X Y Z -0.04345707 0.07975341 0.0000054632487 0.0021943953 1.047 1.047 1.047 0.00952 AUTHOR None FLEXIBLE FIT REAL emd_0071_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 366.45 366.45 366.45 90.0 90.0 90.0 X Y Z -0.0073129637 0.024640752 -0.000013302465 0.001248491 1.047 1.047 1.047 AUTHOR half map 1 emd_0071_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 366.45 366.45 366.45 90.0 90.0 90.0 X Y Z -0.0073460415 0.024740648 -0.000013774461 0.0012522113 1.047 1.047 1.047 AUTHOR half map 2