Cryo-EM structure of a partial yeast 48S preinitiation complex in its open conformation.

2024-09-25 REL PDBe PDBe PDBe 2018-06-14 2016-08-24 2019-07-31 2024-09-25 Wellcome Trust WT096570 United Kingdom Cryo-EM structure of a partial yeast 48S preinitiation complex in its open conformation. Llacer JL Hussain T ribosome, translation, initiation factors, 40S, eIF1A, eIF3, eIF2, tRNAi, 48S PIC, small ribosome subunit Llacer JL Hussain T Dong J Villamayor L Gordiyenko Y Hinnebusch AG Large-scale movement of eIF3 domains during translation initiation modulate start codon selection. Nucleic Acids Res. UK 2021 34648019 doi:10.1093/nar/gkab908 1362-4962 0389 NARHAD Llacer JL Hussain T Marler L Aitken CE Thakur A Lorsch JR Hinnebusch AG Ramakrishnan V Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex. Mol. Cell US 59 399 412 2015 26212456 doi:10.1016/j.molcel.2015.06.033. 1097-4164 2168 MOCEFL EMD-3048 other EM volume EMD-3049 other EM volume

Almost the same complex, at lower resolution

6gsm FULLOVERLAP Structure of a partial yeast 48S preinitiation complex in open conformation Structure of a partial yeast 48S preinitiation complex in open conformation 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 1.8 Ribosome 1 2 3 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) tRNA 1 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Initiation factors eIF1 and eIF1A 1 39 40 41 43 44 45 46 47 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Initiation factors eIF2 and eIF3 1 38 42 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) mRNA 1 4 Kluyveromyces lactis Met-tRNAi Saccharomyces cerevisiae S288C 0.024799072 1 AGCGCCGU(1MG)(2MG)CGCAG(H2U)GGAAGCGC(M2G)CAGGGCUCAU(T6A)ACCCUGAU(7MG)(H2U)(5MC) (5MC)UCGGAUCG(1MA)AACCG(RIA)GCGGCGCUACCA(UNK) 18S ribosomal RNA Kluyveromyces lactis NRRL Y-1140 0.5792396249999999 1 UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACA GUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGAUAUCUGUGGUAAUUCU AGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCCU UGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUC GAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAA CGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGA UACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAACUGGAGGGCAAGUCUG GUGCCAGCAGCCGCGGUAAUUCCAGCUCCAGUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUG GGUCUGGUUGUCCGGUCCGACUUUAUGUCGCGCACUGGUUUUCAACCGGAUCUUUCCUUCUGGCUAACCUGUACUCCUUG UGGGUGCAGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGAAAGCUCGAAUAUAUUAGCAUG GAAUAAUGGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCA UCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUU CAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGG GAUCGGGUGGUGUUUUUCUUAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUC GCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGG AAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCG UUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGGGUUGCUGGCAC UUGCCGGUUGACUCUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCU UAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUACAACCUUGGCCGAGAGGUCUGGGUAAUCUUGU GAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGC UUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAU UUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGAAGAAUCUGGUCAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUA ACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA 40S ribosomal protein S0 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.023140398 1 LEVO SLPSTFDLTSEDAQLLLAARVHLGAKNVQVHQEPYVYKARPDGVNVINVGKTWEKIVLAARIIAAIPNPEDVVAISSRTY GQRAVLKYAAHTGATPIAGRFTPGSFTNYITRSFKEPRLVIVTDPRSDAQAIKESSYVNIPVIALTDLDSPSEYVDVAIP CNNRGKHSIGLIWYLLAREVLRLRGALPDRTQPWAIMPDLYFYRNPEE Q6CN12 mRNA (5'-R(P*AP*AP*U)-3') Kluyveromyces lactis 0.00091962 1 AAU 40S ribosomal protein S1 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.026404724999999997 1 LEVO VGKNKRLSKGKKGLKKRVVDPFTRKEWYDIKAPSTFENRNVGKTLVNKSVGLKNASDSLKGRVVEVCLADLQGSEDHSFR KVKLRVDEVQGKNLLTNFHGMDFTTDKLRSMVRKWQTLIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRTSYAQSSHIR QIRKVISEILTREVQNSTLAQLTSKLIPEVINKEIENATKDIFPLQNVHIRKVKLLKQPKFDLGSLLSLHG Q6CWD0 KLLA0F09812p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.023162922 1 LEVO GWVPVTKLGRLVKAGKISSIEEIFLHSLPVKEFQIIDQLLPNLKDEVMNIKPVQKQTRAGQRTRFKAVVVVGDSNGHVGL GIKTAKEVAGAIRAGIIIAKLSVIPIRRGYWGTNLGQPHSLATKTSGKCGSVSVRLIPAPRGSGIVASPAVKKLMQLAGV EDVYTSSTGSTRTLENTLKAAFVAIGNTYGFLTPNLWEVQALTPSPMDVYADYATAS Q6CKL3 KLLA0D08305p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.024830895 1 LEVO AIISKKRKLVADGVFYAELNEFFTRELAEEGYSGVEVRVTPTKTEIIIRATKVQDVVGENGRRINELTLLIEKRFKYKRG TIALYAERVHDRGLSAVAQAESMKFKLLNGLAIRRAAYGVVRYVMESGAKGCEVVISGKLRAARAKSMKFADGFLIHSGQ PVNDFIETATRHVLLRQGVLGIKVKIMKDPSRNTSGPKALPDAVTIIEPKEEEPVLEPSVKDY Q6CRK7 40S ribosomal protein S4 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.029486312 1 LEVO ARGPKKHLKRLAAPHHWMLDKLSGCYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREVKAILMQRHVKVDGKVRTDTT FPAGFMDVITLEATNENFRLVYDVKGRFAVHRITDEEASYKLAKVKKVQLGKKGIPYVVTHDGRTIRYPDPNIKVNDTVK VDLATGTITDFIKFDTGKLVYVTGGRNLGRVGTIVHRERHEGGFDLVHIKDSLENTFVTRLNNVFVIGEPGRPWISLPKG KGIKLTISEERDRRRAQHGL Q6CWJ2 KLLA0D10659p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.022912369 1 LEVO FVPVELATTIPVEIQQAQQEIKLFNKWSFEDVEVKDASLVDYIQISKPIYVAHTAGRYANKRFRKAQCPIVERLTNSLMM NGRNNGKKLKAVRIVKHTLEIINVLTDQNPLQVVVDAIINSGPREDTTRVGGGGAARRQAVDVSPLRRVNQSIALLTIGA REAAFRNIKTIAETLAEELINAAKGSSTSYAIKKKDELERVAKSNR Q6CRA3 40S ribosomal protein S6 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.025810977 1 LEVO MKLNISYPINGTQKCIEIDDEHRVRVFYDKRIGQEVDGESVGDEFKGYVFKIAGGNDKQGFPMKQGVLLPTRVKLLLAKG HSCYRPRRNGERKRKSVRGAIVGPDLAVLALIITKKGEQEIEGITNDTVPKRLGPKRANNIRKFFGLTKEDDVRDYVIRR EVTKGDKSYTKAPKIQRLVTPQRLQRKRQQKSLKIKNAQAQREAAAEYAQLLAKRLSERKAEKAEV Q6CM04 40S ribosomal protein S7 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.021033569999999998 1 LEVO PQAKILSQAPTELELQVAQAFIDLENNSPELKADLRALQFKSIREIEVAGGKKALAVFVPVPSLAAYHKVQIKLTRELEK KFQDRHVIFLAERRILPKPSRKSRQTQKRPRSRTLTAVHDKILEDLVFPTEIVGKRVRYLVGGNKIQKILLNSKDVQHID NKLESFQAVYNKLTGKQIVFEIPS Q6CTD6 40S ribosomal protein S8 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.022511530999999998 1 LEVO GISRDSRHKRAATGAKRAQFRKKRKFELGRQAANTKIGTKRIHPVRTRGGNQKFRALRIETGNFSWASEGVARKTRITGV VYHPSNNELVRTNTLTKAAIVQIDATPFRQWYESHYGQSLGKKKNTKAEEETATTSKNTERKWAARAAEAKIEHAVDSQF GAGRLYAAISSRPGQSGRCDGYILEGEELAFYLRRLTAKK Q6CMG3 KLLA0E23673p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.020882344 1 LEVO PRAPRTYSKTYSTPKRPYESARLDAELKLAGEYGLKNKREIYRISFQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRLV RIGVLSEDKKKLDYVLALKVEDFLERRLQTQVYKLGLAKSVHHARVLISQRHIAVGKQIVNIPSFMVRLESEKHIDFART SPFGGARPGRVARKRAAAAGGE Q6CM18 KLLA0B08173p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.011423084999999998 1 LEVO MLIPKEDRKKIYQHLFQEGVLVAKKDFNQPKHEEIDTKNLFVIKALQSLTSKGFVKTQFSWQYYYYTLTEEGVVYLREYL NLPEHIFPATYLAGQS Q6CVZ5 KLLA0A10483p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.017712734 1 LEVO STELTVQSERAFQKQPHIFTNPKAKANRKTKRWYKNVGLGFKTPKTAIEGSYIDKKCPFTGLVSIRGKILTGTVVSTRMH RTIVIRRDYLHYVPKYNRYEKRHKNVPAHVSPAFRVQVGDIVTVGQCRPISKTVRFNVLKVASATGKANKQFAKF Q6CX80 40S ribosomal protein S12 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.012710457 1 LEVO IEDALKVVLRTSLVHDGLARGLRESAKALTRGEGQLAVLVESVTEEAISKLVQGLATENNVPLIKVADAKQLGEWAGLGK IDRDGNARKVVGASVVVVKNWGADTQEREILLEHFSQQ Q6CLU4 KLLA0F18040p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.016858678 1 LEVO GRMHSKGKGMSSSAIPYSRNAPAWFKGSSDGVVEQIIKYARKGLTPSQIGVLLRDAHGVTQAKVITGNKILRILKSNGLA PEIPEDLYFLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVSVLPPNWKYESATASALVN Q6CJK0 40S ribosomal protein S14 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.013458438999999999 1 LEVO SQVFGVARIFASFNDTFVHVTDLSGRETIARVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVHIKIRATGGTR SKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDSTRKKGGRRGRRL P27069 KLLA0F07843p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.013448831 1 LEVO KTYSYKGVDLEKLLEMPTEDFVKLAPARVRRKFARGLSEKPAGLMKKLRAAKLSAPENEKPAVVRTHLRNMIIVPEMIGS VVGVYNGKVFNQVEIRPEMVGHYLGEFSITYTPVRHGRA Q6CKV4 40S ribosomal protein S16 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.015656257 1 LEVO TVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVQPEILRFKVYEPLLLVGLDKFANIDIRVKVTGGGHVSQVYAIR QAIAKGLVAYHQKFVDEQSKNELKKAFTSYDRTLLIADSRRPEPKKFGGRGARSRFQKSYR Q875N2 KLLA0B01474p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.014200372999999999 1 LEVO GRVRTKTVKRASKALIEKYYPKLTMDFQTNKRLCDEIATIQSKRLRNKIAGYTTHLMKRIQKGPVRGISFKLQEEERERK DQYVPDVSALDLSHSNDVLNVDTQTAELVNSLGLKLPLSVSSVSA Q6CWU3 KLLA0B01562p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.016953404 1 LEVO SLVVQEQGSFQHILRLLNTNVDGNINVVYALTTIRGVGRRYANLVCKKADVDLHKRAGELTQEELERIVQIMQNPTHYKI PAWFLNRQKDVNDGKDYHSLANNLESKLRDDLERLKKIRSHRGIRHFWGLRVRGQHTKTTGRRRA Q6CWT9 KLLA0A07194p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.015747814 1 LEVO PGVSVRDVPAQDFINNYASFLQRQGKLEVPGYVDIVKTSAGNELPPQDSEGWFYKRAASVARHIYLRKQVGVGKLNKLYG GAKNRGVRPHKHVDASGSINRKVLQSLEKLGVVEISPKGGRRISDNGLRDLDRIAAATLEDEE Q6CXM0 KLLA0F25542p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.012032146 1 LEVO QEVVIHKIRINLTSTKVKQLENVSANIIKNAETFKLVKKGPVRLPTKVLKISTRKTPNGEGSKTWDTYEMRIHKRYIDLE APAHIVKRITQITIEPGVDVEVIIAA Q6CIM1 40S ribosomal protein S21 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.009797949 1 LEVO MENDKGQLVELYVPRKCSATNRIIKAKDHSSVQINIAQVDEEGRAIPGEYVTYALSGYIRARGEADDSLNRLAQQDGLLK NVWSYSR Q6CXT6 40S ribosomal protein S22 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.014513845999999999 1 LEVO TRTSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNV KIADVEKWTANLLPARQFGYVILTTSAGIMDHEEAHRKHVSGKILGFVY Q6CW21 KLLA0B11231p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.015916701 1 LEVO GKGKPRGLNSARKLRVHRRNNRWAETTYKKRLLGTAFKSSPFGGSSHAKGIVLEKIGIESKQPNSAIRKCVRVQLIKNGK KVTAFVPNDGCLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS F2Z602 40S ribosomal protein S24 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.015063354 1 LEVO SDAITIRTRKVISNPLLARKQFVVDVLHPNRANVSKDELREKLAEAYKAEKDAVSVFGFRTQYGGGKSTGFGLVYNSVAD AKKFEPAYRLVRYGLAEKVEKASRQQRKQRKNRGKKIFGTGKSIAKKAARRNAD Q6CU44 KLLA0B06182p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.007939246 1 LEVO AKHAVVLDQDKFDRIMKEAPTYRYVSVSVLVDRFKLGGSLARVALRHLENEGIIKPVSKHSKQAIYTRAT Q6CW78 40S ribosomal protein S26 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.011166179 1 LEVO PKKRASNGRNKKGRGHVKPVRCVNCSRSVPKDKAIKRMAIRNIVEAAAIRDLSEASVYAEYALPKTYNKLHYCISCAIHA RIVRVRSRTDRRIRAPPQ Q6CS01 40S ribosomal protein S27 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.008753167999999999 1 LEVO VLVQDLLHPTAASEARKHKLKTLVQSPRSHFLDVKCPGCLNITTVFSHAQTAVTCESCSTVLCTPTGGKAKLSEGTSFRR K Q6CNL2 40S ribosomal protein S28 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.006972152 1 LEVO PVTLAKVIKVLGRTGSRGGVTQVRVEFLEDTTRTIVRNVKGPVREGDILVLMESEREARRLR P33285 40S ribosomal protein S29 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.006322149 1 LEVO ENVWYSHPRKFGKGSRQCRISGSHSGLIRKYGLNIDRQSFREKANDIGFYKYR Q6CPG3 40S ribosomal protein S30 Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.006608787999999999 1 LEVO VHGSLARAGKVKSQTPKVEKQEKPKQPKGRAYKRLLYTRRFVNVTLTNGKRKMNPSPS Q6CUH5 Ubiquitin-40S ribosomal protein S27a Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.007958424 1 LEVO KKVYTTPKKIRHKHKKVKLAVLNYYKVDDEGKVAKLRKECPNCGPGIFLANHGDRFYCGKCHSTFATQK P69061 KLLA0E12277p Kluyveromyces lactis NRRL Y-1140 ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 0.035612675999999996 1 LEVO SSNIMLVLRGTLEGHNGWVTSLSTSAAQPNLLVSGSRDKTLISWRLTENEQQFGVPVRSYKGHSHIVQDVVVSADGNYAV SASWDKTLRLWNLATGNSEARFVGHTGDVLSVAIDANSSKIISASRDKTIRVWNTVGDCAYVLLGHTDWVTKVRVAPKNL EDGEVDDGRITFVSAGMDKIVRSWSLNEDSYRIEADFIGHNNYINVVQPSPDGSLAASAGKDGQIYVWNLKHKSAFMNFD AKDEVFALAFSPSRFWLTAATASGIKIYDLENEVLIDELKPEFAGYTKAQDPHAVSLAWSADGQTLFAGYTDNVIRVWQV MTAN Q6CNI7 60S ribosomal protein L41-A Kluyveromyces lactis NRRL Y-1140 ATCC 204508 / S288c 0.003354243 1 LEVO MRAKWRKKRTRRLKRKRRKVRARSK P0CX86 Eukaryotic translation initiation factor 1A Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.010953534999999999 1 Escherichia coli BL21(DE3) LEVO GPKRELIYKEEGQEYAQITKMLGNGRVEASCFDGNKRMAHIRGKLRKKVWMGQGDIILVSLRDFQDDQCDVVHKYNLDEA RTLKNQGELPENAKI P38912 Eukaryotic translation initiation factor 2 subunit alpha Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.030221979 1 Saccharomyces cerevisiae LEVO TSHCRFYENKYPEIDDIVMVNVQQIAEMGAYVKLLEYDNIEGMILLSELSRRRIRSIQKLIRVGKNDVAVVLRVDKEKGY IDLSKRRVSSEDIIKCEEKYQKSKTVHSILRYCAEKFQIPLEELYKTIAWPLSRKFGHAYEAFKLSIIDETVWEGIEPPS KDVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAAPLYVLTTQALDKQKGIE QLESAIEKITEVITKYGGVCNIT P20459 Eukaryotic translation initiation factor 2 subunit gamma Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.047230148 1 Saccharomyces cerevisiae LEVO LSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDCYRSFKSDKE ISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHVIILQN KVDLMREESALEHQKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAE IEDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVPGGLIGVGTKVDPTLCRA DRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTS PACTEINEKIALSRRIEKHWRLIGWATIKK P32481 Eukaryotic translation initiation factor 2 subunit beta Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.016458089999999998 1 Saccharomyces cerevisiae LEVO VGLPYSELLSRFFNILRTNNPELAGDRSGPKFRIPPPVCLRDGKKTIFSNIQDIAEKLHRSPEHLIQYLFAELGTSGSVD GQKRLVIKGKFQSKQMENVLRRYILEYVTCKTCKSINTELKREQSNRLFFMVCKSCGSTRSVSS P09064 Eukaryotic translation initiation factor eIF-1 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.010919531 1 Escherichia coli BL21(DE3) LEVO ADTGDDETATSNYIHIRIQQRNGRKTLTTVQGVPEEYDLKRILKVLKKDFACNGNIVKDPEMGEIIQLQGDQRAKVCEFM ISQLGLQKKNIKIHGF P32911 Eukaryotic translation initiation factor 3 subunit A,Eukaryotic translation initiation factor 3 subunit A,eIF3a Saccharomyces cerevisiae S288C 0.062822781 1 Saccharomyces cerevisiae LEVO PFRPENAIKRADELISVGEKQAALQSLHDFITARRIRWATPSTVEPVVFKFLEIGVELKKGKLLKDGLHQYKKLIQGSTE GLVSVGAVARKFIDLVESKIASEQTRADELQKQEIDDDLEGGVTPENLLISVYESDQSVAGFNDEAITSWLRFTWESYRA VLDLLRNNALLEITYSGVVKKTMHFCLKYQRKNEFKRLAEMLRQHLDAANYQQSKSGNNLVDLSDADTLQRYLDQRFQQV DVSVKLELWHEAYRSIEDVFHLMKISKRAPKPSTLANYYENLVKVFFVSGDPLLHTTAWKKFYKLYSTNPRATEEEFKTY SSTIFLSAISTQLDEIPSIGYDPHLRMYRLLNLDAKPTRKEMLQSIIEDESIYGKVDEELKELYDIIEVNFDVDTVKQQL ENLLVKLSSKTYFSQYIAPLRDVIMRRVFVAASQKFTTVSQSELYKLATLPAPLDLSAWDIEKSLLQAAVEDYVSITIDH ESAKVTFA(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) P38249 Eukaryotic translation initiation factor 3 subunit B Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.075014 1 Saccharomyces cerevisiae LEVO QYIVVNGAPVIPSAKVPVLKKALTSLFSKAGKVVNMEFPIDEATGKTKGFLFVECGSMNDAKKIIKSFHGKRLDLKHRLF LYTMKDVERYNSDDFDTEFREPDMPTFVPSSSLKSWLMDDKVRDQFVLQDDVKTSVFWNSMFNEEDSLVESRENWSTNYV RFSPKGTYLFSYHQQGVTAWGGPNFDRLRRFYHPDVRNSSVSPNEKYLVTFSTEPIIVEEDNEFSPFTKKNEGHQLCIWD IASGLLMATFPVIKSPYLKWPLVRWSYNDKYCARMVGDSLIVHDATKNFMPLEAKALKPSGIRDFSFAPEGVKLQPFRNG DEPSVLLAYWTPETNNSACTATIAEVPRGRVLKTVNLVQVSNVTLHWQNQAEFLCFNVERHTKSGKTQFSNLQICRLTER DIPVEKVELKDSVFEFGWEPHGNRFVTISVHEVADMNYAIPANTIRFYAPETKEKTDVIKRWSLVKEIPKTFANTVSWSP AGRFVVVGALVGPNMRRSDLQFYDMDYPGEKNINDNNDVSASLKDVAHPTYSAATNITWDPSGRYVTAWSSSLKHKVEHG YKIFNIAGNLVKEDIIAGFKNFAWRPRPLSNAERKKVRKNLREWSEEQDAMEADTAMRHQRELLKQWTEYREKIGQEMEK SMNFKIFDVQP P06103 Eukaryotic translation initiation factor 3 subunit C Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.07696317999999999 1 Saccharomyces cerevisiae LEVO YDSSDEESDEEDGKKVVKSAKEKLLDEMQDVYNKISQAENSDWLTISNEFDLISRLLVRAQQQNWGTPNIFIKVVAQVED AVNNTQQADLKAVARAYNTTKQRVKKVSRENEDSMAKFRNDQEDFFTRLQTIIDSRGKKTVNQQSLISTLEELLTVAEKP YEFIMAYLTLIPSRFDASANLSYQPIDQWKSSFNDISKLLSILDQTIDTYQVNEFADPIDFIEDEPKEDSDGVKRILGSI FSFVERLDDEFMKSLLNIDPHSSDYLIRLRDEQSIYNLILRTQLYFEATLKDEHDLERALTRPFVKRLDHIYYKSENLIK IMETAAWNIIPAQFKSKFTSKDQLDSADYVDNLIDGLSTILSKQNNIAVQKRAILYNIYYTALNKDFQTAKDMLLTSQVQ TNINQFDSSLQILFNRVVVQLGLSAFKLCLIEECHQILNDLLSSSHLREILGQQSLHRISLNSSNNASADERARQCLPYH QHINLDLIDVVFLTCSLLIEIPRMTAFYSGIKVKRIPYSPKSIRRSLEHYDKLSFQGPPETLRDYVLFAAKSMQKGNWRD SVKYLREIKSWALLPNMETVLNSLTERVQVESLKTYFFSFKRFYSSFSVAKLAELFDLPENKVVEVLQSVIAELEIPAKL NDEKTIFVVEKGDEITKLEEAMVKL P32497 Eukaryotic translation initiation factor 3 subunit I Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.038229754 1 Saccharomyces cerevisiae LEVO MKAIKLTGHERPLTQVKYNKEGDLLFSCSKDSSASVWYSLNGERLGTLDGHTGTIWSIDVDCFTKYCVTGSADYSIKLWD VSNGQCVATWKSPVPVKRVEFSPCGNYFLAILDNVMKNPGSINIYEIERDSATHELTKVSEEPIHKIITHEGLDAATVAG WSTKGKYIIAGHKDGKISKYDVSNNYEYVDSIDLHEKSISDMQFSPDLTYFITSSRDTNSFLVDVSTLQVLKKYETDCPL NTAVITPLKEFIILGGGQEAKDVTTTSANEGKFEARFYHKIFEEEIGRVQGHFGPLNTVAISPQGTSYASGGEDGFIRLH HFEKSYFDFKYDVEKAAEAKEH P40217 Eukaryotic translation initiation factor 3 subunit G Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.005576158 1 Saccharomyces cerevisiae LEVO SVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQ Q04067 [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate 0.00047841699999999997 1 7NO MAGNESIUM ION 2.4305e-05 82 MG ZINC ION 6.5409e-05 4 ZN PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER 0.0005212079999999999 1 GCP singleParticle particle 0.15 6.5 20.0 MES 5.0 Magnessium acetate 80.0 Potassium acetate 10.0 Ammonium acetate 2.0 Dithiothreitol (DTT) 0.001 Zinc acetate 0.6 ATP 0.25 GDPCP Quantifoil R2/2 CARBON CONTINUOUS ETHANE 100 277 FEI VITROBOT MARK I FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 1.5 3.5 59000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 90.0 100.0 FEI FALCON III (4k x 4k) INTEGRATING 2 2610 1.1 40.0

Images were collected in movie-mode at 40 frames per second

FEI Falcon III

360729 4V88 C1 5.15 FSC 0.143 CUT-OFF RELION 1.4 5750 NOT APPLICABLE RELION 1.4 ANGULAR RECONSTITUTION RELION 1.4 RELION 1.4 emd_0057.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 402.0 402.0 402.0 90.0 90.0 90.0 X Y Z -0.11824102 0.24851649 0.00020422891 0.017586468 1.34 1.34 1.34 0.07 AUTHOR ::::EMDATABANK.org::::EMD-0057:::: For optimal visualization of eIF2, and of eIF3 at the 40S subunit interface, gauss-filter the map by 1.34 and display it at 0.04 or 0.03 contour level, respectively. OTHER FSC RECIPROCAL emd_0057_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 402.0 402.0 402.0 90.0 90.0 90.0 X Y Z -0.058553785 0.10654058 0.000101078746 0.00846327 1.34 1.34 1.34 AUTHOR ::::EMDATABANK.org::::EMD-0057:::: emd_0057_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 402.0 402.0 402.0 90.0 90.0 90.0 X Y Z -0.058553785 0.10654058 0.000101078746 0.00846327 1.34 1.34 1.34 AUTHOR ::::EMDATABANK.org::::EMD-0057::::