2025-07-09 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name DATABASE_REFERENCES citation _citation.journal_volume _citation.page_first _citation.page_last DERIVED_CALCULATIONS pdbx_struct_assembly pdbx_struct_assembly_gen pdbx_struct_oper_list _pdbx_struct_assembly.oligomeric_count _pdbx_struct_assembly.oligomeric_details _pdbx_struct_assembly_gen.oper_expression OTHER atom_sites cell _atom_sites.fract_transf_matrix[1][1] _atom_sites.fract_transf_matrix[2][2] _atom_sites.fract_transf_matrix[3][3] _cell.Z_PDB DERIVED_CALCULATIONS chem_comp_atom chem_comp_bond database_2 pdbx_struct_oper_list struct_sheet _database_2.pdbx_DOI _database_2.pdbx_database_accession _pdbx_struct_oper_list.name _pdbx_struct_oper_list.symmetry_operation _pdbx_struct_oper_list.type _struct_sheet.number_strands STRUCTURE_SUMMARY em_admin em_software pdbx_entry_details _em_admin.last_update _em_software.name PDBe PDBe 2018-06-14 2018-08-22 2019-01-16 2025-07-09 Medical Research Council (United Kingdom) MC_UU_12014/7 United Kingdom Biotechnology and Biological Sciences Research Council BB/J013854/1 United Kingdom Feline Calicivirus Strain F9 Conley MJ Bhella D Capsid, Calicivirus, Vesivirus, Vp1, VIRUS Conley MJ McElwee M Azmi L Gabrielsen M Byron O Goodfellow IG Bhella D Calicivirus VP2 forms a portal-like assembly following receptor engagement. Nature UK 565 377 381 2019 30626974 doi:10.1038/s41586-018-0852-1 1476-4687 0006 NATUAS 6gsh FULLOVERLAP T=3 Icosahedral Capsid. T=3 Icosahedral Capsid. 0 1
T=3 Icosahedral Capsid
Feline calicivirus VP1 Feline calicivirus 0.073346664 3 Felis catus LEVO MCSTCANVLKYYNWDPHFRLVIDPNKFLSVGFCDNPLMCCYPELLPEFGTVWDCDQSPLQIYLESILGDDEWASTYEAID PAVPPMHWDAAGKIFQPHPGVLMHHLIGEVAKAWDPNLPMFRLEADGDGSITAPEQGTPVGGVIAEPSAQMSAAADMATG KSVDSEWEAFFSFHTSVNWSTSETQGKILFKQSLGPLLNPYLEHLAKLYVAWSGSIDVRFSISGSGVFGGKLAAIVVPPG VDPVQSTSMLQYPHVLFDARQVEPVIFSIPDLRSTLYHLMSDTDTTSLVIMVYNDLINPYANDSNSSGCIVTVETKPGAD FKFHLLKPPGSMLTHGSVPSDLIPKSSSLWIGNRHWTDITDFVIRPFVFQANRHFDFNQETAGWSTPRYRPITITISEKN GAKLGIGVATDYIVPGIPDGWPDTTIPEKLTPAGDYAITNKSGNDITTAAGYDGADVIVNNTNFKGMYICGSLQRAWGDK KISNTAFITTATKVDNAIEPSNVIDMTKIAVYQDTHVGKEVQTSDDTLSLLGYTGIGEQAIGSDRDRVVRISVLPETGAR GGNHPIFYKNSIKLGYVIRSIDVFNSQILHTSRQLSLNHYLLPPDSFAVYRIIDSNGSWFDIGIDSDGFSFVGVSSIGKL EFPLTASYMGIQLAKIRLASNIRSSMTKL A2T4P8 POTASSIUM ION 3.9098e-05 3 K singleParticle particle 1 7.2
Phosphate buffered saline
 ETHANE 100 277 FEI VITROBOT MARK IV
Purified enveloped virions
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 75000.0 FEI FALCON III (4k x 4k) INTEGRATING 1 5198 63.0
Each micrograph was recorded as a movie of 50 individual fractions with a total dose of 63 e/angstrom squared
 1
Images were motion-corrected using motioncor2 Defocus estimation was performed using GCTF
59531
Autopicking in Relion
 Gctf
CTF correction was implemented through Relion
PHASE FLIPPING AND AMPLITUDE CORRECTION
Previously published models calculated at lower resolution.
 I 3.0 FSC 0.143 CUT-OFF RELION 2.1 41436 PROJECTION MATCHING RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 10 RELION 2.1
Origin and orientation values were determined by 3D auto refine in RELION, assuming full icosahedral symmetry (i.e. with full gold-standard methods).
 emd_0054.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 545.28 545.28 545.28 90.0 90.0 90.0 X Y Z -0.25493634 0.45862737 0.0027425927 0.027666343 1.065 1.065 1.065 0.05 AUTHOR Icosahedral reconstruction of feline calicivirus strain F9