2019-04-17 REL PDBe PDBe PDBe 2018-05-30 2018-08-22 2019-02-20 2019-04-17 cryoEM structure of the lambdaN Transcription Antitermination Complex at 3.7 A resolution. Krupp F Spahn C Krupp F Said N Huang YH Loll B Burger J Mielke T Spahn CMT Wahl MC Structural Basis for the Action of an All-Purpose Transcription Anti-termination Factor. Mol.Cell US 74 143 157.e5 2019 30795892 doi:10.1016/j.molcel.2019.01.016 1097-2765 2168 MOCEFL EMD-0043 associated EM volume 6gov FULLOVERLAP Transcription Antitermination Complex Transcription Antitermination Complex 0
Consisting of bacterial RNA Polymerase, NusA, NusB, NusE, NusG, DNA, RNA and lambdaN
Escherichia coli Escherichia coli 0.5 singleParticle particle 7.5 10.0 HEPES-NaOH HEPES-NaOH 50.0 NaCl sodium chloride 1.0 DTT DTT Quantifoil R2/4 COPPER/RHODIUM 300 CARBON HOLEY GLOW DISCHARGE OTHER
A thin carbon support film was placed over the holey carbon film.
 ETHANE 75 4 FEI VITROBOT MARK II
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 1-40 1 20.0 69.0 1 805023 SPHIRE CTER 1 C1 FOURIER SPACE 3.7 FSC 0.143 CUT-OFF SPHIRE sxmeridien 708030 MAXIMUM LIKELIHOOD SPHIRE sxmeridien MAXIMUM LIKELIHOOD SPHIRE sxmeridien SPARX own script emd_0043.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 -120 -120 -120 240 240 240 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -0.08612833 0.15245315 0.00035626657 0.0039406037 1.35 1.35 1.35 0.0138 AUTHOR cryoEM of the lambdaN Transcription Antitermination Complex, 3.7A map RIGID BODY FIT Cross-correlation coefficient REAL 97.430000000000007 emd_0043_msk_1.map emd_0043_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 -120 -120 -120 240 240 240 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -0.018787894 0.059323285 -0.0001833529 0.0027398015 1.35 1.35 1.35 AUTHOR cryoEM of the lambdaN Transcription Antitermination Complex, 2nd half map emd_0043_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 -120 -120 -120 240 240 240 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -0.015402886 0.05807875 -0.00012171921 0.002778478 1.35 1.35 1.35 AUTHOR cryoEM of the lambdaN Transcription Antitermination Complex, 1st half map