2024-11-20 REL PDBe PDBe PDBe 2018-05-23 2018-08-22 2019-08-14 2024-11-20 Knut and Alice Wallenberg Foundation Sweden Swedish Research Council NT_2015-04107 Sweden Neurturin-GFRa2-RET extracellular complex Bigalke JM Aibara S Neurotrophic signalling, Receptor tyrosine kinase, GDNF family of ligands, SIGNALING PROTEIN Bigalke JM Aibara S Roth R Dahl G Gordon E Dorbeus S Amunts A Sandmark J Cryo-EM structure of the activated RET signaling complex reveals the importance of its cysteine-rich domain. Sci Adv US 5 eaau4202 eaau4202 2019 31392261 doi:10.1126/sciadv.aau4202 2375-2548 6gl7 FULLOVERLAP Neurturin-GFRa2-RET extracellular complex Neurturin-GFRa2-RET extracellular complex 0 1 2 3 0.254 Neurturin 1 1 Homo sapiens GDNF family receptor alpha-2 1 2 Homo sapiens Proto-oncogene tyrosine-protein kinase receptor Ret 1 3 Homo sapiens Neurturin Homo sapiens 0.011706406 2 Escherichia coli LEVO ARLGARPCGLRELEVRVSELGLGYASDETVLFRYCAGACEAAARVYDLGLRRLRQRRRLRRERVRAQPCCRPTAYEDEVS FLDAHSRYHTVHELSARECACV Q99748 GDNF family receptor alpha-2 Homo sapiens 0.047635629 2 Cricetulus griseus LEVO SPSSLQGPELHGWRPPVDCVRANELCAAESNCSSRYRTLRQCLAGRDRNTMLANKECQAALEVLQESPLYDCRCKRGMKK ELQCLQIYWSIHLGLTEGEEFYEASPYEPVTSRLSDIFRLASIFSGTGADPVVSAKSNHCLDAAKACNLNDNCKKLRSSY ISICNREISPTERCNRRKCHKALRQFFDRVPSEYTYRMLFCSCQDQACAERRRQTILPSCSYEDKEKPNCLDLRGVCRTD HLCRSRLADFHANCRASYQTVTSCPADNYQACLGSYAGMIGFDMTPNYVDSSPTGIVVSPWCSCRGSGNMEEECEKFLRD FTENPCLRNAIQAFGNGTDVNVSPKGPSFQATQAPRVEKTPSLPDDLSDSTSLGTSVITTCTSVQEQGLKANNSKELSMC FTELTTNIIPGSNKVIKPNSHHHHHH O00451 Proto-oncogene tyrosine-protein kinase receptor Ret Homo sapiens 0.068651352 2 Cricetulus griseus LEVO LYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSSWEK LSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQF RLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDS APTFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVR ATVHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVE NCQAFSGINVQYKLHSSGANCSTLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSY VAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRG SIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRENLYFQ P07949 2.7.10.1 singleParticle particle 8.0 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 QUANTUM (4k x 4k) COUNTING 38.0 1 C2 6.3 FSC 0.143 CUT-OFF 186903 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0026.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -0.033035327 0.098938644 0.0002195305 0.002489064 1.05 1.05 1.05 0.0367 AUTHOR Consensus refinement with C2 symmetry applied. FLEXIBLE FIT REAL 220.0 emd_0026_msk_1.map emd_0026_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -0.042894214 0.103082314 0.00011629565 0.0019309446 1.05 1.05 1.05 AUTHOR Symmetry expanded refinement as C1 emd_0026_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -0.006969529 0.032559678 0.0000070670044 0.0012181341 1.05 1.05 1.05 AUTHOR Half map one for consensus refinement map (C2_all_bfacauto.mrc) emd_0026_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -0.0059396815 0.031567723 0.000007872128 0.0012183752 1.05 1.05 1.05 AUTHOR Half map two for consensus refinement map (C2_all_bfacauto.mrc)