2019-11-27 REL PDBe PDBe PDBe 2018-05-22 2018-06-27 2018-06-27 2019-11-27 European Research Council 693023 Germany Volkswagen Foundation Germany German Research Foundation SFB 860 Germany Influenza hemagglutinin (HA) trimer reconstruction at 3.2 Angstrom resolution using data from EMPIAR-10097 processed with Warp and cryoSPARC Tegunov D Cramer P Tegunov D Cramer P Real-time cryo-electron microscopy data preprocessing with Warp. Nat.Methods US 16 1146 1152 2019 31591575 doi:10.1038/s41592-019-0580-y 1548-7105 Tegunov D Cramer P Real-time cryo-EM data pre-processing with Warp Biorxiv US 2018 doi:10.1101/338558 EMD-8731 other EM volume
Original publication, based on the same raw data, but with different software and processing strategy
 EMD-0025 associated EM volume Influenza hemagglutinin (HA) Trimer A/Hong Kong/1/1968 H3N2 Influenza hemagglutinin (HA) Trimer A/Hong Kong/1/1968 H3N2 0 1 Influenza A virus (A/Hong Kong/1/1968(H3N2)) Homo sapiens HEK293 0.15 Influenza hemagglutinin (HA) Trimer A/Hong Kong/1/1968 H3N2 LEVO QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIKGVELKSGYKD singleParticle particle 0.75 7.4
Same as EMD-8731
 C-flat-1.2/1.3 4C GOLD 400 HOLEY GLOW DISCHARGE OTHER
Same as EMD-8731
 ETHANE GATAN CRYOPLUNGE 3
Same as EMD-8731.
Same as EMD-8731
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 1.0 3.5 22500.0 38167.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN
Same as EMD-8731
GATAN K2 SUMMIT (4k x 4k) COUNTING 3710 3838 5.0 2 847 20.0 82.0
Same as EMD-8731
 1 489215 Warp 6x6x1 model resolution
On-the-fly correction in cryoSPARC, based on local defocus values from Warp
6 ab initio models created in cryoSPARC
 1 C3 FOURIER SPACE 3.2 FSC 0.143 CUT-OFF cryoSPARC
Mask used for FSC automatically generated in cryoSPARC.
249495 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC 6 81500 cryoSPARC
3D classification initialized with the 6 ab initio models obtained earlier.
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