2020-01-15 REL PDBe PDBe PDBe 2018-05-18 2018-06-20 2019-06-19 2020-01-15 European Research Council 322408 United Kingdom National Institutes of Health/National Institute of General Medical Sciences AG-058504 United States National Institutes of Health/National Institute of General Medical Sciences EB-002026 United States Wellcome Trust 204963 United Kingdom Wellcome Trust 092896MA United Kingdom Four protofilament beta-2-microglobulin amyloid fibril Iadanza MG Ranson NA Iadanza MG Ranson NA n/a Not Published 0353 Iadanza MG Silvers R Boardman J Smith HI Karamanon TK Debelouchina GT Su Y Griffin RG Ranson NA Radford SE The structure of a Beta 2-microglobulin fibril suggests a molecular basis for its amyloid polymorphism Nat Commun. UK 2018 30375379 doi:10.1038/s41467-018-06761-6 2041-1723 EMD-0019 associated EM volume
Two protofilament beta-2-microglobulin amyloid fibril
 four protofilament beta-2-microglobulin amyloid fibril four protofilament beta-2-microglobulin amyloid fibril 0 1 Homo sapiens Escherichia coli 0.0117 Human beta-2-microglobulin Homo sapiens Escherichia coli LEVO IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM helical filament 0.025 2.5 20.0 CH3COONa Sodium Acetate 20.0 Na3PO4 Sodium phosphate 0.01 NaN3 Sodium Azide Quantifoil R3.5/1 COPPER 400 CARBON HOLEY PLASMA CLEANING ETHANE 80 281.15 FEI VITROBOT MARK II
Quiescent growth at 0.25 mg/ml for 5 weeks, diluted 10x with buffer
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 0.00175 0.00325 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 3-40 1 5549 10.0 38.5 1 1 2.3 -0.161 C1 FOURIER SPACE 9.42 FSC 0.5 CUT-OFF RELION
Resolution estimated using rmeasure software
8891 RELION 2.1 Gctf
Double protofilament map from same study Filtered to 60 Angstrom resolution
 NOT APPLICABLE RELION 2.1 emd_0019.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 600 600 600 0 0 0 600 600 600 635.99994 635.99994 635.99994 90.0 90.0 90.0 X Y Z -0.005038559 0.019340014 0.00028363764 0.0018114602 1.06 1.06 1.06 0.0089 AUTHOR Four protofilament beta-2-microglobulin amyloid fibril