2024-10-23 REL PDBe PDBe PDBe 2018-05-18 2018-06-20 2018-11-14 2024-10-23 European Research Council 322408 United Kingdom National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) EB-002026 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) AG-058504 United States Wellcome Trust 092896MA United Kingdom Wellcome Trust 204963 United Kingdom Two protofilament beta-2-microglobulin amyloid fibril Iadanza MG Ranson NA amyloid, b2m, PROTEIN FIBRIL Iadanza MG Silvers R Boardman J Smith HI Karamanos TK Debelouchina GT Su Y Griffin RG Ranson NA Radford SE The structure of a beta2-microglobulin fibril suggests a molecular basis for its amyloid polymorphism. Nat Commun UK 9 4517 4517 2018 30375379 doi:10.1038/s41467-018-06761-6 2041-1723 6gk3 FULLOVERLAP two protofilament beta-2-microglobulin amyloid fibril two protofilament beta-2-microglobulin amyloid fibril 0 1 Homo sapiens 53.3 Beta-2-microglobulin Homo sapiens 0.007635446 8 Escherichia coli LEVO FLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHV P61769 helical filament 0.025 2.5 20.0 CH3COONa Sodium Acetate 20.0 Na3PO4 Sodium phosphate 0.01 NaN3 Sodium Azide Quantifoil R3.5/1 COPPER 400 CARBON HOLEY PLASMA CLEANING ETHANE 80 281.15 FEI VITROBOT MARK II
Quiescent growth at 0.25 mg/ml for 5 weeks, diluted 10x with buffer
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 0.00175 0.0032500000000000003 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 3-40 1 5549 10.0 38.5 1 1 4.83 -0.608 C2 FOURIER SPACE 3.975 FSC 0.143 CUT-OFF RELION 11035 99000 RELION 2.1
300 x 300 pixel Overlapping segments 90% overlap
 EMD-1613
Filtered to 60 Angstrom resolution
 NOT APPLICABLE RELION 2.1 emd_0014.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 317.99997 317.99997 317.99997 90.0 90.0 90.0 X Y Z -0.08127573 0.13223831 0.000266917 0.0030406867 1.06 1.06 1.06 0.0398 AUTHOR Beta-2-microglobulin amyloid fibril - two protofilamets OTHER REAL